FastQCFastQC Report
Thu 26 May 2016
SRR938509_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938509_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences969399
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51080.5269244139925872No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43060.44419274210103377No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28010.28894191143172215No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10400.10728296604390969No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24650.049.467121
GTATCAA43700.037.4947661
TATCAAC51250.031.6219712
ATCAACG51550.031.5301343
TCAACGC52350.031.0482984
CAACGCA53400.030.52685
AACGCAG53850.030.0874236
GCGCCGT404.4737896E-429.71122494-95
ACGCAGA61100.026.3577027
CGCAGAG61250.026.2155938
GCAGAGT65150.024.0629339
CGCCGTG500.001646381123.76897894-95
GAGTACT44100.023.10490212-13
GTACATG34400.022.431081
GTACTTT45800.022.30088214-15
AGGACGT3500.021.7260285
TAGGTCG1100.00286803221.6025855
CGACTAG1105.664515E-821.58364160-61
TACATGG34650.021.5340042
GGACGTG3550.021.41456
Produced by FastQC (version 0.11.5)