FastQCFastQC Report
Thu 26 May 2016
SRR938509_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938509_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences969399
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47310.4880343388016699No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41400.4270687302132558No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26800.2764599509593057No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT12640.1303900664225979No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10680.1101713535912457No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27450.040.2191281
GTATCAA42850.034.3158381
ATACCGG702.0547274E-433.9239856
ACCGGTC700.0083964727.1391878
TATCAAC53400.027.0906412
ATCAACG52950.026.9488533
TCAACGC53700.026.4825294
CAACGCA54750.025.9746485
AACGCAG55350.025.7417776
GACGTGG2251.0186341E-1025.3299087
ATAGCCC6100.023.3924883
GTGTAGC7050.022.9496881
ACGCAGA62350.022.5470717
CGCAGAG62950.022.256728
GGACGTG3650.022.1202966
GTACATG34950.021.785111
TAGCCCT11750.021.4536194
TAGGACG3800.021.2778594
GTATAGC7750.020.8768121
GCAGAGT66550.020.8386559
Produced by FastQC (version 0.11.5)