##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938509_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 969399 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.771646143641576 31.0 31.0 34.0 30.0 34.0 2 32.00023210257077 31.0 31.0 34.0 30.0 34.0 3 32.06486080550939 33.0 31.0 34.0 30.0 34.0 4 35.75322957832636 37.0 35.0 37.0 33.0 37.0 5 35.62069178944893 37.0 35.0 37.0 33.0 37.0 6 35.65373081672253 37.0 35.0 37.0 33.0 37.0 7 35.59475613240781 37.0 35.0 37.0 33.0 37.0 8 35.58339857994489 37.0 35.0 37.0 33.0 37.0 9 37.14513115858382 39.0 37.0 39.0 33.0 39.0 10-11 37.09298699503506 39.0 37.0 39.0 33.0 39.0 12-13 37.001792863413314 39.0 37.0 39.0 33.0 39.0 14-15 38.04479631194173 40.0 37.0 41.0 33.0 41.0 16-17 37.97625074917552 40.0 37.0 41.0 33.0 41.0 18-19 38.028733266694104 40.0 37.0 41.0 33.0 41.0 20-21 38.051166753834075 40.0 37.0 41.0 33.0 41.0 22-23 38.04780126655794 40.0 37.0 41.0 33.0 41.0 24-25 37.99743707183523 40.0 37.0 41.0 33.0 41.0 26-27 37.84318170330277 40.0 37.0 41.0 32.5 41.0 28-29 37.710865185542794 40.0 37.0 41.0 32.0 41.0 30-31 37.484012259142006 39.0 36.5 41.0 32.0 41.0 32-33 37.25545621565527 39.0 36.0 41.0 31.5 41.0 34-35 37.07437855826136 39.0 36.0 41.0 31.0 41.0 36-37 37.06835008082327 39.0 36.0 41.0 31.0 41.0 38-39 36.92749683051045 39.0 36.0 41.0 31.0 41.0 40-41 36.75259929090086 39.0 35.0 41.0 30.0 41.0 42-43 36.5272524522926 39.0 35.0 41.0 30.0 41.0 44-45 36.20143202128329 39.0 35.0 40.0 30.0 41.0 46-47 35.78996471009357 39.0 35.0 40.0 28.5 41.0 48-49 35.3940271240222 38.5 35.0 40.0 27.0 41.0 50-51 35.04877712892215 38.0 34.5 40.0 26.0 41.0 52-53 34.58819278748998 38.0 34.0 40.0 24.0 41.0 54-55 34.325522823935245 38.0 33.5 40.0 23.5 41.0 56-57 34.00644780941594 38.0 33.0 40.0 23.0 41.0 58-59 33.60804838874395 38.0 33.0 40.0 22.0 41.0 60-61 33.269429306198994 37.0 32.0 40.0 19.5 41.0 62-63 32.834186439226784 37.0 31.5 39.5 18.0 41.0 64-65 32.4097023000849 36.0 31.0 39.0 15.0 40.0 66-67 31.961813453490258 36.0 31.0 39.0 12.0 40.0 68-69 31.446531304447394 35.0 30.0 38.5 8.0 40.0 70-71 30.948135391103147 35.0 29.5 38.0 7.5 40.0 72-73 30.398832163020593 34.5 29.0 37.5 6.5 39.5 74-75 29.833655698014958 34.0 28.5 37.0 2.0 39.0 76-77 28.479988116348377 32.5 27.0 35.5 2.0 37.5 78-79 29.132126193651942 34.0 28.0 36.0 2.0 38.0 80-81 28.854255059062368 34.0 28.0 35.5 2.0 37.5 82-83 28.397879510913462 33.5 27.5 35.0 2.0 37.0 84-85 27.840371714846 33.0 26.0 35.0 2.0 36.5 86-87 27.227120102248918 33.0 26.0 35.0 2.0 36.0 88-89 26.543277329561924 32.0 25.0 35.0 2.0 35.5 90-91 26.05529921116073 32.0 24.5 34.0 2.0 35.0 92-93 25.34285521235322 31.5 20.5 34.0 2.0 35.0 94-95 24.623707575518438 31.0 18.5 34.0 2.0 35.0 96-97 23.722128865410426 31.0 4.5 34.0 2.0 35.0 98-99 22.60318970826254 31.0 2.0 34.0 2.0 35.0 100-101 19.420446070194007 26.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28764395164514056 1101 2 0.3141123305111293 1101 3 0.313273613959268 1101 4 0.2759898895992663 1101 5 0.08983470266340277 1101 6 0.1836055163241852 1101 7 0.3400799764934348 1101 8 0.3990216598142453 1101 9 0.48343073628846156 1101 10-11 0.3268528305701963 1101 12-13 0.3503817987458717 1101 14-15 0.48753482466381826 1101 16-17 0.5770037814356073 1101 18-19 0.5320976324526541 1101 20-21 0.6291270665999207 1101 22-23 0.48691709778842807 1101 24-25 0.4531379631468795 1101 26-27 0.3993461154001139 1101 28-29 0.5660407879269798 1101 30-31 0.43385003625181184 1101 32-33 0.34524344366841575 1101 34-35 0.17520444346015296 1101 36-37 0.38781524120426525 1101 38-39 0.4174632042797768 1101 40-41 0.523372565205463 1101 42-43 0.533749824930041 1101 44-45 0.7911386191814671 1101 46-47 0.9119458695832847 1101 48-49 0.7125343271026878 1101 50-51 0.5835167828916141 1101 52-53 0.6486766940161885 1101 54-55 0.501504474403788 1101 56-57 0.5414809987792921 1101 58-59 1.07256648571002 1101 60-61 0.955726428167786 1101 62-63 1.009719590457827 1101 64-65 1.120546004936351 1101 66-67 1.1205083952822577 1101 68-69 1.1683912563741607 1101 70-71 1.3549484473084128 1101 72-73 1.4068308724406258 1101 74-75 1.4078837312777885 1101 76-77 1.1530541932414202 1101 78-79 1.5118643535897718 1101 80-81 1.820570946813703 1101 82-83 1.6008210920113193 1101 84-85 1.521868161887582 1101 86-87 1.8345506135058756 1101 88-89 1.918431748967837 1101 90-91 2.31003210419809 1101 92-93 2.5506896703244593 1101 94-95 2.4913842293366883 1101 96-97 3.1758357773317982 1101 98-99 3.444096660346723 1101 100-101 3.8278124850677315 1102 1 0.3837127107686449 1102 2 0.39470922229853755 1102 3 0.38233081076616315 1102 4 0.09651649463750545 1102 5 0.1759991779146688 1102 6 0.20899231300758458 1102 7 0.3178828302791672 1102 8 0.25172288258012543 1102 9 0.392936214050728 1102 10-11 0.3882426239548309 1102 12-13 0.3112543551103357 1102 14-15 0.7122122335019583 1102 16-17 0.6098996300995339 1102 18-19 0.5804846433762378 1102 20-21 0.6327205797796864 1102 22-23 0.5698593208031468 1102 24-25 0.4756085650021191 1102 26-27 0.674815747186436 1102 28-29 0.499116678188102 1102 30-31 0.5723603084699178 1102 32-33 0.4490241841410523 1102 34-35 0.6564391322729577 1102 36-37 0.758074179078605 1102 38-39 0.9389145018966545 1102 40-41 1.1130240988857452 1102 42-43 1.022638610321252 1102 44-45 0.944029597540549 1102 46-47 0.7858901378599086 1102 48-49 0.9121729132239196 1102 50-51 1.0739192217699483 1102 52-53 1.1958474594125406 1102 54-55 1.060533633690703 1102 56-57 1.02191261956618 1102 58-59 1.1686585318102516 1102 60-61 1.1513869333221791 1102 62-63 1.204185141035147 1102 64-65 1.1761346797476975 1102 66-67 1.237295244926898 1102 68-69 1.3021569734201002 1102 70-71 1.3337227006946684 1102 72-73 1.1160610316037847 1102 74-75 1.0538132342804793 1102 76-77 1.1507674121270206 1102 78-79 1.651451814816955 1102 80-81 1.8402963467841644 1102 82-83 1.8065189494763914 1102 84-85 2.003337051152016 1102 86-87 1.7764143434408162 1102 88-89 1.891029630784434 1102 90-91 2.3047393070595916 1102 92-93 2.578109087404858 1102 94-95 2.7882911865538524 1102 96-97 3.2244949132119736 1102 98-99 3.63865469348875 1102 100-101 4.350837316969024 1103 1 0.23513917010016527 1103 2 0.238217636704114 1103 3 0.207309676416628 1103 4 0.11181813830924625 1103 5 0.039033608485986804 1103 6 0.1481066557699009 1103 7 0.12103556325961762 1103 8 0.0617934469577861 1103 9 0.16940285049871306 1103 10-11 0.17027287011224956 1103 12-13 0.3080907583874364 1103 14-15 0.23657948293973163 1103 16-17 0.38114292923081194 1103 18-19 0.17484561182268266 1103 20-21 0.45017411855013734 1103 22-23 0.3578128040061799 1103 24-25 0.397134883354731 1103 26-27 0.42011343246007726 1103 28-29 0.22124399875217193 1103 30-31 0.42400966182749755 1103 32-33 0.4149482453326385 1103 34-35 0.37040303717650147 1103 36-37 0.43995632870105794 1103 38-39 0.4458429472950982 1103 40-41 0.557988629391005 1103 42-43 0.5787685706498635 1103 44-45 0.6216049600711244 1103 46-47 0.6120446602360943 1103 48-49 0.6352661079009323 1103 50-51 0.6078172199693128 1103 52-53 0.8644246463026235 1103 54-55 0.7352881693031748 1103 56-57 0.9303924573342925 1103 58-59 1.0333081636292718 1103 60-61 0.9384038870039362 1103 62-63 0.7849102396517083 1103 64-65 0.9615220720624151 1103 66-67 1.0119038068046464 1103 68-69 0.8913782678537423 1103 70-71 0.8715603664135685 1103 72-73 0.745844668310248 1103 74-75 0.7523587414986217 1103 76-77 0.8004366923354063 1103 78-79 1.2268842729322316 1103 80-81 1.3775084107828448 1103 82-83 1.1799625935002709 1103 84-85 1.3462335996036856 1103 86-87 1.4528827280772383 1103 88-89 1.6546445847883469 1103 90-91 1.9620388054543803 1103 92-93 1.9861275282954836 1103 94-95 1.8820883642284905 1103 96-97 2.2982091518322036 1103 98-99 2.805275158032366 1103 100-101 3.425799012911604 1104 1 0.10608708854564952 1104 2 0.17029542737653003 1104 3 0.1660003712516911 1104 4 0.13792571210780835 1104 5 -0.014116690704433665 1104 6 0.0544149212321372 1104 7 0.15407022892259192 1104 8 0.15379610023261847 1104 9 0.23685457983214775 1104 10-11 0.15472786260199456 1104 12-13 0.06510875163343854 1104 14-15 0.2945734027118547 1104 16-17 0.27945651982957287 1104 18-19 0.350702321041652 1104 20-21 0.4048320447440261 1104 22-23 0.34510752080626617 1104 24-25 0.3120111122658926 1104 26-27 0.41479875104473507 1104 28-29 0.340884157065787 1104 30-31 0.46181766649279865 1104 32-33 0.5165173251550925 1104 34-35 0.6061129334037147 1104 36-37 0.4480288360573894 1104 38-39 0.45734356937420984 1104 40-41 0.1519516159747809 1104 42-43 0.3236063596029979 1104 44-45 0.4365041902264508 1104 46-47 0.36393035566941734 1104 48-49 0.5362207757013948 1104 50-51 0.5040246865449092 1104 52-53 0.6504642851133866 1104 54-55 0.6894076572815422 1104 56-57 0.8616071205112732 1104 58-59 0.7746620772677346 1104 60-61 0.5033365154304548 1104 62-63 0.6684716686562737 1104 64-65 0.6483978874158609 1104 66-67 0.6853215709675524 1104 68-69 0.9308619944734993 1104 70-71 0.6811745379680012 1104 72-73 0.9445132680107697 1104 74-75 1.0923386212183779 1104 76-77 0.9613820899374694 1104 78-79 1.3236683969623755 1104 80-81 1.5739477484864182 1104 82-83 1.31592376089473 1104 84-85 1.3651840690962516 1104 86-87 1.5497981218341437 1104 88-89 1.6718531166235806 1104 90-91 1.7707608075384336 1104 92-93 1.8692788846137987 1104 94-95 1.9049101289815233 1104 96-97 2.5951168785077208 1104 98-99 2.8204780510777425 1104 100-101 3.09258765587305 1105 1 0.23488154940138273 1105 2 0.2544964797782967 1105 3 0.18577745990383931 1105 4 0.13908415605025937 1105 5 0.09177622451974798 1105 6 0.157036346207299 1105 7 0.2239411069785433 1105 8 0.12136301150917461 1105 9 0.23117263507210595 1105 10-11 0.2530892213676239 1105 12-13 0.3999307968216641 1105 14-15 0.4271021744493808 1105 16-17 0.4526901155950185 1105 18-19 0.37983161675229127 1105 20-21 0.46235714064981437 1105 22-23 0.29615268229780867 1105 24-25 0.2911117810444921 1105 26-27 0.292690405347237 1105 28-29 0.4573829607496336 1105 30-31 0.5072420356977929 1105 32-33 0.3870016215563368 1105 34-35 0.5637456309295459 1105 36-37 0.5070821868564934 1105 38-39 0.5197034979071233 1105 40-41 0.23908553655007836 1105 42-43 0.34063827027805615 1105 44-45 0.15893211050768485 1105 46-47 0.20345300993182747 1105 48-49 0.47194328827720256 1105 50-51 0.7660903257609064 1105 52-53 0.5389139306740987 1105 54-55 0.5295501904420021 1105 56-57 0.5136300532457767 1105 58-59 0.7209587324836235 1105 60-61 0.6164145678188504 1105 62-63 0.7639124120208365 1105 64-65 0.9067498226855761 1105 66-67 0.9409188602087255 1105 68-69 1.1756818564404448 1105 70-71 1.0934396745975512 1105 72-73 1.1291981935361868 1105 74-75 1.2474169498511039 1105 76-77 1.0009334825207539 1105 78-79 1.2751167860569623 1105 80-81 1.4690194606220075 1105 82-83 1.5593195165343445 1105 84-85 1.5758554541942047 1105 86-87 1.6380197544381012 1105 88-89 1.798106241080557 1105 90-91 1.9185130921235505 1105 92-93 2.398503417783168 1105 94-95 2.268174757677958 1105 96-97 2.676420707164951 1105 98-99 2.9918865406896202 1105 100-101 3.617823911604006 1106 1 0.23139030720553322 1106 2 0.3714123951789148 1106 3 0.31196925603465786 1106 4 0.26532764984434465 1106 5 0.17841490845123076 1106 6 0.12576145983216236 1106 7 0.31762717236838967 1106 8 0.2362165201305899 1106 9 0.19030838213633672 1106 10-11 0.30640546147358094 1106 12-13 0.22823240758885532 1106 14-15 0.15134848851598548 1106 16-17 0.16131823560399283 1106 18-19 0.32606714690633254 1106 20-21 0.5455051323605886 1106 22-23 0.3425942331397067 1106 24-25 0.23771414461766227 1106 26-27 0.3463894281514044 1106 28-29 0.38393983037911994 1106 30-31 0.5110852619839648 1106 32-33 0.5432424094727253 1106 34-35 0.6535890200083614 1106 36-37 0.706413527079917 1106 38-39 0.6579760479878658 1106 40-41 0.9097571221944065 1106 42-43 0.805102726484634 1106 44-45 0.583886902551086 1106 46-47 0.40606770174775164 1106 48-49 1.0010584222731467 1106 50-51 0.6544508718642206 1106 52-53 0.7093831254664948 1106 54-55 0.7868648598189409 1106 56-57 0.8020793653368656 1106 58-59 1.1980935159914168 1106 60-61 1.0198911806759483 1106 62-63 1.1734079528223944 1106 64-65 0.7193171946403609 1106 66-67 0.9044819071590027 1106 68-69 0.725513454052237 1106 70-71 1.1717148865579752 1106 72-73 1.3657601009379725 1106 74-75 1.4340185654580786 1106 76-77 1.4644442818326233 1106 78-79 1.7211416757319498 1106 80-81 1.3094386736735757 1106 82-83 1.8058821719023257 1106 84-85 1.7438052128651123 1106 86-87 2.1244689169359567 1106 88-89 2.517792161276965 1106 90-91 2.350354122908964 1106 92-93 2.2623532217720275 1106 94-95 2.6254507088049692 1106 96-97 2.9975280931111072 1106 98-99 3.420001489893913 1106 100-101 3.8674444287566274 1107 1 0.24425160478113028 1107 2 0.36984622925086796 1107 3 0.2994460384903377 1107 4 0.21171650705237255 1107 5 0.1497106918193225 1107 6 0.20671329768408953 1107 7 0.24713137596588552 1107 8 0.25683736541213165 1107 9 0.3581834473083916 1107 10-11 0.3470967536724814 1107 12-13 0.4143958713206075 1107 14-15 0.44405604114242436 1107 16-17 0.4926982742184336 1107 18-19 0.3974035609769757 1107 20-21 0.525048744684625 1107 22-23 0.27429952103725697 1107 24-25 0.3109685518778278 1107 26-27 0.35774718606980827 1107 28-29 0.38575395560722825 1107 30-31 0.40497904782859706 1107 32-33 0.47977093853945973 1107 34-35 0.5542148753915725 1107 36-37 0.77923820610755 1107 38-39 0.6639112873322333 1107 40-41 0.5903575401102046 1107 42-43 0.6617522108107536 1107 44-45 0.6795202730868013 1107 46-47 0.7895120651797143 1107 48-49 0.9414947288099 1107 50-51 0.7913717570636507 1107 52-53 0.8694782766656601 1107 54-55 0.8769509683955832 1107 56-57 0.7322770403238366 1107 58-59 0.9508052844351056 1107 60-61 1.0118770546331959 1107 62-63 0.9163159095155677 1107 64-65 0.9634934606075163 1107 66-67 1.1404775528511308 1107 68-69 1.1240950453908702 1107 70-71 0.9396764065332057 1107 72-73 1.2558031348600274 1107 74-75 1.2066918212909776 1107 76-77 1.105028040142063 1107 78-79 1.423694383630803 1107 80-81 1.3890402280270706 1107 82-83 1.281662607251647 1107 84-85 1.786735266957919 1107 86-87 2.02521442413747 1107 88-89 2.010694008090052 1107 90-91 2.0371922192332583 1107 92-93 2.2386871104255732 1107 94-95 2.2636648519553866 1107 96-97 2.5217323830615754 1107 98-99 2.846976203136876 1107 100-101 3.2226127763191954 1108 1 0.2343946944841484 1108 2 0.2012670088273012 1108 3 0.1824506179694012 1108 4 0.13875958610543648 1108 5 0.14257142088455765 1108 6 0.19054819301027948 1108 7 0.2202896950992823 1108 8 0.18035359898077985 1108 9 0.20382761722076026 1108 10-11 0.23089675139034682 1108 12-13 0.31136377312805763 1108 14-15 0.3364254461144327 1108 16-17 0.35251971637398327 1108 18-19 0.3265210033150936 1108 20-21 0.2979218923538127 1108 22-23 0.18616404224587058 1108 24-25 0.2857158057118099 1108 26-27 0.3371970590311051 1108 28-29 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13 4420.0 14 5478.0 15 7616.0 16 9833.0 17 12422.0 18 11551.0 19 8796.0 20 8051.0 21 8425.0 22 9002.0 23 10053.0 24 11415.0 25 13339.0 26 15648.0 27 18668.0 28 22587.0 29 27520.0 30 32866.0 31 39426.0 32 47943.0 33 58657.0 34 73552.0 35 95300.0 36 125885.0 37 163063.0 38 112325.0 39 7657.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.4610485465737 21.25120822282672 15.483820387683503 22.803922842916073 2 15.21318689484211 22.32492089473065 40.17618853990147 22.28570367052578 3 18.060833756277102 25.784509117017194 31.733986936195326 24.42067019051038 4 12.347237824672813 19.154960960347598 38.25638359437136 30.241417620608235 5 12.387458786088311 38.47089546613412 35.798509535605824 13.343136212171746 6 29.48620743367798 38.411943895135025 18.380666784265305 13.721181886921691 7 26.006938319515495 34.0670869270548 22.14423575844415 17.781738994985552 8 24.176525868089406 36.49931555530798 21.10049628687465 18.223662289727965 9 24.950923200869816 16.822175389081277 22.021788757776726 36.205112652272184 10-11 23.00554260938994 27.906929963822947 29.223673636964758 19.863853789822354 12-13 24.265807990311522 25.831778246109188 28.870929307746344 21.031484455832945 14-15 21.12932858399895 27.76421267197511 27.75405173721037 23.35240700681556 16-17 19.94632756996861 30.38160757335215 28.91146989010717 20.76059496657207 18-19 19.609417793911486 28.92348764543805 31.507253463228245 19.959841097422217 20-21 21.10926460621478 28.022826514159803 31.36969400628637 19.498214873339048 22-23 20.238673652438262 28.73718664863488 30.806922639697383 20.21721705922948 24-25 19.93268945597091 28.507691712550447 31.511908292607444 20.047710538871193 26-27 19.98413245377468 28.78391455873159 31.259581913886063 19.97237107360767 28-29 19.695295745095674 28.869743005717975 31.24709227057177 20.187868978614585 30-31 20.26781541965692 28.644448777025765 31.317084090245608 19.770651713071707 32-33 19.820186446570172 28.588714793690485 31.276427620170562 20.314671139568784 34-35 20.180946175786456 28.937408078610165 31.181283038178943 19.70036270742444 36-37 20.148757559736424 28.748584765761002 30.8187597518988 20.28389792260377 38-39 19.836372546490935 28.913827345180675 31.068221093085036 20.181579015243354 40-41 20.621077836157102 28.342640439643425 31.072616062005654 19.963665662193822 42-43 20.208903950605823 28.503886645105712 30.901278698502598 20.385930705785864 44-45 20.32181742875137 28.689730799246316 30.69711807520249 20.291333696799825 46-47 20.387869785098474 28.564494956919116 30.246983306641496 20.80065195134091 48-49 20.346936607114305 28.569092809049728 30.30666423216859 20.777306351667374 50-51 20.46551523160226 28.85344424741515 29.76364737326942 20.91739314771317 52-53 20.318001153291885 28.991725801243863 29.841014896858773 20.849258148605475 54-55 20.498329634304156 28.801416548509206 29.643639844707824 21.05661397247881 56-57 20.655271977792424 28.917195086852782 29.28113191781712 21.14640101753767 58-59 20.405271719900682 29.027314862094965 29.416267192353203 21.151146225651154 60-61 20.568207724579867 28.872270344821892 29.331523964848323 21.22799796574991 62-63 20.29840138064925 28.788352370901972 29.422198702495052 21.49104754595373 64-65 20.216907589135126 28.725529941747414 29.30924211805459 21.748320351062876 66-67 20.136693222694106 28.846695419936314 29.1601892723002 21.856422085069376 68-69 20.330493599897256 28.58133161955584 29.124451915285615 21.963722865261293 70-71 20.066144074833993 28.32920190757366 29.276438288052702 22.328215729539643 72-73 20.001103776669872 28.089001536003234 29.39934949386166 22.51054519346523 74-75 20.067743003654844 28.15558918463914 28.96046932171376 22.816198489992253 76-77 19.749298276561046 28.084204749540692 29.463306646695532 22.70319032720273 78-79 19.61256407320412 27.95469151505211 29.357106825981873 23.0756375857619 80-81 19.462471077440764 28.030150639726266 29.2955738555538 23.21180442727917 82-83 19.56560423151613 27.876943549697366 29.477378873882355 23.080073344904154 84-85 19.60548156049513 27.661939487847125 29.31132841393127 23.421250537726475 86-87 19.19748294670604 27.717347294038763 29.31490245129012 23.77026730796508 88-89 19.57743921749455 27.416729334360774 29.175447880594056 23.830383567550616 90-91 19.378776203252965 27.391780217104934 29.29131941328233 23.938124166359774 92-93 19.139743128843563 27.241072151591222 29.344545807623607 24.274638911941604 94-95 19.00414586769741 27.23290409831246 29.245388121918836 24.517561912071294 96-97 19.02802664331199 27.101224573163375 29.238837671588275 24.631911111936365 98-99 19.071919818361685 26.832295061166768 29.12603582219499 24.96974929827656 100-101 18.87920180691043 26.722488741625526 29.325054583322803 25.073254868141237 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 431.0 1 245.0 2 74.5 3 90.0 4 72.0 5 76.0 6 136.0 7 257.0 8 372.5 9 450.5 10 473.5 11 461.5 12 533.5 13 638.0 14 716.5 15 838.5 16 1010.5 17 1196.0 18 1426.0 19 1680.5 20 1965.5 21 2398.0 22 2930.0 23 3600.0 24 4540.0 25 5654.5 26 7318.0 27 9440.0 28 11415.5 29 13497.5 30 16007.5 31 18764.5 32 21461.0 33 23998.0 34 27117.5 35 30533.0 36 33328.0 37 35630.5 38 36491.5 39 36776.0 40 37226.5 41 37367.5 42 37690.5 43 38242.0 44 38724.5 45 38974.0 46 39269.5 47 39195.5 48 38730.0 49 37492.0 50 35625.0 51 32974.0 52 29437.5 53 26107.0 54 22906.0 55 19450.5 56 16007.5 57 12907.5 58 10104.0 59 7738.5 60 5842.0 61 4138.5 62 2848.0 63 2083.5 64 1430.0 65 907.5 66 600.0 67 431.5 68 329.5 69 228.5 70 143.5 71 93.0 72 76.0 73 76.0 74 60.0 75 34.0 76 21.5 77 16.0 78 11.0 79 10.0 80 8.5 81 7.0 82 3.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.045079477078065894 3 3.094700943574318E-4 4 0.0 5 0.10068093736428448 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0011863020283701552 26-27 0.012894587264892992 28-29 0.0 30-31 0.0 32-33 6.189401887148636E-4 34-35 0.016556650048122602 36-37 0.003765219481348753 38-39 0.012791430566773846 40-41 0.007014655472101786 42-43 0.005570461698433772 44-45 0.0030431225945147457 46-47 1.547350471787159E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 5.157834905957196E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.0631339623828783E-4 68-69 5.157834905957196E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.705185377744355E-4 84-85 0.004126267924765757 86-87 0.0011863020283701552 88-89 0.0 90-91 6.189401887148636E-4 92-93 2.5789174529785983E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 9.799886321318672E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 969399.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.41894547892956 #Duplication Level Percentage of deduplicated Percentage of total 1 77.08240910885631 52.738971462033824 2 14.337966602276378 19.61977110479721 3 4.581087840861643 9.402995976542995 4 1.960756724529989 5.366116297322473 5 0.8587243055961291 2.9376505723006603 6 0.43951727847889266 1.8042785227976914 7 0.24405179546534259 1.16884365415847 8 0.14214520089890376 0.7780339800314867 9 0.08910233952786818 0.5486659299182055 >10 0.24506025688346833 2.608100576798848 >50 0.00996974568865311 0.47174560589142 >100 0.008001089944114153 0.9207085989749718 >500 4.5289162214913134E-4 0.20017193213580756 >1k 7.548193702485521E-4 1.4339457862958804 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4731 0.4880343388016699 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4140 0.4270687302132558 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2680 0.2764599509593057 No Hit CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 1264 0.1303900664225979 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1068 0.1101713535912457 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 5.1578349059571964E-5 0.0 16-17 0.0 0.0 0.0 1.0315669811914393E-4 0.0 18-19 0.0 0.0 0.0 1.0315669811914393E-4 0.0 20-21 0.0 0.0 0.0 6.189401887148635E-4 0.0 22-23 0.0 0.0 0.0 0.0017020855189658747 0.0 24-25 0.0 0.0 0.0 0.003455749386991321 0.0 26-27 0.0 0.0 0.0 0.0057251967456124885 0.0 28-29 0.0 0.0 0.0 0.01578297481222902 0.0 30-31 0.0 0.0 0.0 0.04466685028558932 0.0 32-33 0.0 0.0 0.0 0.10372405995879921 0.0 34-35 0.0 0.0 0.0 0.20920178378562387 0.0 36-37 0.0 0.0 0.0 0.3742009224271946 0.0 38-39 0.0 0.0 0.0 0.5943373162134478 0.0 40-41 0.0 0.0 0.0 0.8068916926879438 0.0 42-43 0.0 0.0 0.0 1.0167124166622825 0.0 44-45 0.0 0.0 0.0 1.2965249603104605 0.0 46-47 0.0 0.0 0.0 1.6198180522158574 0.0 48-49 0.0 0.0 0.0 1.9434721925646714 0.0 50-51 0.0 0.0 0.0 2.2599053640451454 0.0 52-53 0.0 0.0 0.0 2.5624639596285945 0.0 54-55 0.0 0.0 0.0 2.91850930318682 0.0 56-57 0.0 0.0 0.0 3.3386149562770333 0.0 58-59 0.0 0.0 0.0 3.774245692434178 0.0 60-61 0.0 0.0 0.0 4.21988262830888 0.0 62-63 0.0 0.0 0.0 4.647002936871195 0.0 64-65 0.0 0.0 0.0 5.126475269728976 0.0 66-67 0.0 0.0 0.0 5.675011011977524 0.0 68-69 0.0 0.0 0.0 6.270844100313699 0.0 70-71 0.0 0.0 0.0 6.905515685491732 0.0 72-73 0.0 0.0 0.0 7.516873856894839 0.0 74-75 0.0 0.0 0.0 8.146645498912212 0.0 76-77 0.0 0.0 0.0 8.83609329079151 0.0 78-79 0.0 0.0 0.0 9.575004719418938 0.0 80-81 0.0 0.0 0.0 10.348834690359697 0.0 82-83 0.0 0.0 0.0 11.137673960876791 0.0 84-85 0.0 0.0 0.0 11.933424730167868 0.0 86-87 0.0 0.0 0.0 12.758420423375721 0.0 88-89 0.0 0.0 0.0 13.664652016352399 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2745 0.0 40.219128 1 GTATCAA 4285 0.0 34.315838 1 ATACCGG 70 2.0547274E-4 33.923985 6 ACCGGTC 70 0.00839647 27.139187 8 TATCAAC 5340 0.0 27.090641 2 ATCAACG 5295 0.0 26.948853 3 TCAACGC 5370 0.0 26.482529 4 CAACGCA 5475 0.0 25.974648 5 AACGCAG 5535 0.0 25.741777 6 GACGTGG 225 1.0186341E-10 25.329908 7 ATAGCCC 610 0.0 23.392488 3 GTGTAGC 705 0.0 22.949688 1 ACGCAGA 6235 0.0 22.547071 7 CGCAGAG 6295 0.0 22.25672 8 GGACGTG 365 0.0 22.120296 6 GTACATG 3495 0.0 21.78511 1 TAGCCCT 1175 0.0 21.453619 4 TAGGACG 380 0.0 21.277859 4 GTATAGC 775 0.0 20.876812 1 GCAGAGT 6655 0.0 20.838655 9 >>END_MODULE