FastQCFastQC Report
Thu 26 May 2016
SRR938508_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938508_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences963283
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47220.49019862283461874No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39690.41202844854523535No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24920.2586986378873083No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9800.10173541939388529No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24450.048.569081
GTATCAA41450.037.1635631
TATCAAC49750.030.860422
TCAACGC50600.030.2480754
ATCAACG50750.030.0650123
CAACGCA52100.029.377215
AACGCAG52350.029.3185716
CGACCCG350.00836271127.16294994-95
TGACGCG806.2016625E-826.7385394-95
CGCGACT458.9626695E-426.38237292-93
ACGCAGA58700.026.0592527
TAGCCCT10950.025.6112214
CGCAGAG59750.025.6013098
GTGGTAT11800.025.0581511
GTAGCCC6450.025.055993
GCAGAGT62500.023.8655419
CGACGAG500.001646941323.7675894-95
GCGCCGT601.7028362E-423.7675894-95
GTACATG33150.022.874571
GTATAGC7750.022.768761
Produced by FastQC (version 0.11.5)