##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938508_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 963283 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.233995617072033 31.0 31.0 34.0 28.0 34.0 2 31.421827230419307 31.0 31.0 34.0 28.0 34.0 3 31.464145012421064 31.0 31.0 34.0 28.0 34.0 4 35.135959006854684 37.0 35.0 37.0 32.0 37.0 5 35.146621501677075 37.0 35.0 37.0 32.0 37.0 6 35.133095881480315 37.0 35.0 37.0 32.0 37.0 7 35.07033031829691 37.0 35.0 37.0 32.0 37.0 8 35.05034865143473 37.0 35.0 37.0 32.0 37.0 9 36.573701601709985 39.0 37.0 39.0 32.0 39.0 10-11 36.44006174717087 39.0 37.0 39.0 32.0 39.0 12-13 36.31341101213246 39.0 35.0 39.0 32.0 39.0 14-15 37.476027813217925 40.0 37.0 41.0 32.0 41.0 16-17 37.419380389771234 40.0 37.0 41.0 32.0 41.0 18-19 37.472443196859075 40.0 37.0 41.0 32.0 41.0 20-21 37.45392735052938 40.0 37.0 41.0 32.0 41.0 22-23 37.324360546173864 40.0 37.0 41.0 32.0 41.0 24-25 37.23041826752886 39.0 36.5 41.0 31.0 41.0 26-27 37.049660380179034 39.0 36.0 41.0 31.0 41.0 28-29 36.84704443034913 39.0 36.0 41.0 30.5 41.0 30-31 36.609339622935316 39.0 36.0 40.0 30.0 41.0 32-33 36.32408388812011 39.0 35.0 40.0 30.0 41.0 34-35 36.06531310113435 38.0 35.0 40.0 29.5 41.0 36-37 35.88240786975375 38.0 35.0 40.0 29.0 41.0 38-39 35.69566368346581 38.0 35.0 40.0 28.0 41.0 40-41 35.46484574107505 38.0 35.0 40.0 27.0 41.0 42-43 35.10360039573002 38.0 34.0 40.0 25.5 41.0 44-45 34.64574585038872 38.0 33.5 40.0 24.5 41.0 46-47 34.15880224191645 38.0 33.0 40.0 23.5 41.0 48-49 33.72372397312109 38.0 33.0 40.0 20.0 41.0 50-51 33.02187519140273 37.5 32.0 39.5 18.0 40.5 52-53 33.08358654725558 37.5 32.5 39.5 18.0 40.0 54-55 33.20554810995315 38.0 33.0 40.0 16.0 41.0 56-57 32.9121992187135 38.0 32.0 40.0 12.0 41.0 58-59 32.58139767856383 37.0 31.5 40.0 8.5 41.0 60-61 32.16389627970181 37.0 31.0 39.5 7.5 41.0 62-63 31.56643686227204 36.0 30.5 39.0 2.0 40.0 64-65 31.03790682488947 36.0 29.0 39.0 2.0 40.0 66-67 30.48092772321322 35.0 28.5 38.5 2.0 40.0 68-69 29.75454876708091 34.5 28.0 38.0 2.0 40.0 70-71 29.05339604249219 34.0 26.5 38.0 2.0 39.5 72-73 28.26611286610477 34.0 26.0 37.0 2.0 39.0 74-75 27.43654824179395 33.0 24.5 36.0 2.0 39.0 76-77 26.55120302133433 32.0 22.0 36.0 2.0 38.5 78-79 25.623796952712755 31.5 19.0 35.0 2.0 37.5 80-81 24.721532509138022 31.0 15.5 35.0 2.0 36.5 82-83 23.757784057229287 30.0 7.5 34.0 2.0 36.0 84-85 22.79362451117688 29.5 2.0 34.0 2.0 35.5 86-87 21.808707825218548 29.0 2.0 34.0 2.0 35.0 88-89 20.737651344412804 28.0 2.0 33.5 2.0 35.0 90-91 19.57855116305385 26.0 2.0 33.0 2.0 34.5 92-93 18.3739508534875 24.5 2.0 32.0 2.0 34.0 94-95 17.03681368818924 19.5 2.0 32.0 2.0 34.0 96-97 15.496417978932463 4.5 2.0 31.0 2.0 34.0 98-99 13.626238083719944 2.0 2.0 31.0 2.0 34.0 100-101 9.691180058196812 2.0 2.0 23.0 2.0 30.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7021962026488104 1101 2 0.7122981945027753 1101 3 0.6858129519531424 1101 4 0.5798528625489325 1101 5 0.4622105562069905 1101 6 0.5926315700166782 1101 7 0.5502015383643126 1101 8 0.7094232012800319 1101 9 0.681237483374801 1101 10-11 0.8080659815489284 1101 12-13 0.8605458297894089 1101 14-15 0.9333939205311879 1101 16-17 0.8590516669638362 1101 18-19 0.8071707920733076 1101 20-21 0.8653280862944754 1101 22-23 0.9325603549942869 1101 24-25 0.9461970407696825 1101 26-27 1.0673626483055116 1101 28-29 1.1409228394235669 1101 30-31 1.1149435987435155 1101 32-33 1.2482732859937258 1101 34-35 1.3028521282846413 1101 36-37 1.3446271805628527 1101 38-39 1.396765338470182 1101 40-41 1.3225328876224083 1101 42-43 1.3188427954982558 1101 44-45 1.3687735051292336 1101 46-47 1.2946994842868662 1101 48-49 1.2585193774774694 1101 50-51 1.3479157268485622 1101 52-53 1.3449180785548407 1101 54-55 1.4816593027626723 1101 56-57 1.6658242666733756 1101 58-59 1.392643285708715 1101 60-61 1.5556145567901964 1101 62-63 1.322469167567494 1101 64-65 1.27379910100613 1101 66-67 1.5239797477703405 1101 68-69 1.5321172084990238 1101 70-71 1.929991614415254 1101 72-73 1.917548527667094 1101 74-75 1.9339163409179712 1101 76-77 2.092741608556441 1101 78-79 2.545013965768156 1101 80-81 2.966648244026235 1101 82-83 2.9241543487543282 1101 84-85 2.9633858823800097 1101 86-87 3.2114679081893556 1101 88-89 3.2854567092142872 1101 90-91 3.2190572299256566 1101 92-93 3.842533507271135 1101 94-95 3.8102941048612706 1101 96-97 3.815424102566947 1101 98-99 3.732815864058418 1101 100-101 2.945916390858981 1102 1 0.6175521629137108 1102 2 0.5911536642108963 1102 3 0.527386106233255 1102 4 0.3587650528849622 1102 5 0.30921815983711554 1102 6 0.4754881434775555 1102 7 0.5068562477094147 1102 8 0.5513106184985759 1102 9 0.672829033534228 1102 10-11 0.70978216379077 1102 12-13 0.8024033537040509 1102 14-15 0.8255557429496321 1102 16-17 0.7908451118988395 1102 18-19 0.6978149544476011 1102 20-21 0.9110686737362172 1102 22-23 0.5867599809447412 1102 24-25 0.7610195205342123 1102 26-27 0.6808460613543232 1102 28-29 0.7300623721682342 1102 30-31 0.5773546853267888 1102 32-33 0.8222170559226001 1102 34-35 0.8423189543822573 1102 36-37 0.9004395119339534 1102 38-39 0.9509527005010838 1102 40-41 0.8123423369724136 1102 42-43 1.0463193407521132 1102 44-45 0.7666342176629612 1102 46-47 0.8375043959866488 1102 48-49 1.0678039310707916 1102 50-51 1.0392098769589282 1102 52-53 0.93336010087517 1102 54-55 1.1344332480655908 1102 56-57 1.365503871775168 1102 58-59 1.4938321194099657 1102 60-61 1.2113663350585284 1102 62-63 1.4152802112270457 1102 64-65 1.6666660987986184 1102 66-67 1.750325712940814 1102 68-69 1.7475789450700319 1102 70-71 1.7433424238339477 1102 72-73 1.6737762993129124 1102 74-75 1.6875648569837018 1102 76-77 2.0671866661968643 1102 78-79 2.410041866185125 1102 80-81 2.453380216064499 1102 82-83 2.70932497053505 1102 84-85 2.6344637276093437 1102 86-87 2.8818635728937956 1102 88-89 2.891796389125986 1102 90-91 2.668778992250896 1102 92-93 2.7154524734227152 1102 94-95 2.6360123106498428 1102 96-97 2.6031356551791625 1102 98-99 2.6532421885617286 1102 100-101 1.831685645212671 1103 1 0.45083750699663483 1103 2 0.479679434438367 1103 3 0.520545238587605 1103 4 0.4257868400046476 1103 5 0.21567271401697496 1103 6 0.39823744763342717 1103 7 0.5617655480261448 1103 8 0.5677565346133662 1103 9 0.5459214608305132 1103 10-11 0.47448607011575916 1103 12-13 0.5588399119150154 1103 14-15 0.5827397337356999 1103 16-17 0.5011963932118277 1103 18-19 0.677893416872017 1103 20-21 0.4632497851672639 1103 22-23 0.505139863850971 1103 24-25 0.44942771709818885 1103 26-27 0.5027843471782987 1103 28-29 0.4608654722738095 1103 30-31 0.41431960518473687 1103 32-33 0.6491690186829331 1103 34-35 0.5755071202331123 1103 36-37 0.581679314218249 1103 38-39 0.6061403384701762 1103 40-41 0.729804425464593 1103 42-43 0.6932540273823093 1103 44-45 0.6246964117314846 1103 46-47 0.7710128900839663 1103 48-49 0.5805654724855174 1103 50-51 0.7402164514862406 1103 52-53 1.01140861800733 1103 54-55 0.9311711787691124 1103 56-57 0.7582658930824024 1103 58-59 0.9856535514091931 1103 60-61 1.3121161670961499 1103 62-63 1.3296601901117704 1103 64-65 1.4851215647742428 1103 66-67 1.4911093773999724 1103 68-69 1.8027089959386373 1103 70-71 1.7792971699708104 1103 72-73 1.682247601741171 1103 74-75 1.458815696795586 1103 76-77 1.8459622204727033 1103 78-79 2.192427411822905 1103 80-81 2.28745138412285 1103 82-83 2.207834904309884 1103 84-85 2.3059533139420054 1103 86-87 2.4078497519896764 1103 88-89 2.6886722889244297 1103 90-91 2.7798513120512602 1103 92-93 2.7108155121020516 1103 94-95 2.822758879418437 1103 96-97 2.4966439093302313 1103 98-99 2.587571298841029 1103 100-101 2.2754554407784653 1104 1 0.6048898275170913 1104 2 0.5008390074442772 1104 3 0.43370669000099227 1104 4 0.3803202160984753 1104 5 0.41386531950558947 1104 6 0.4503460745633703 1104 7 0.4319813358119191 1104 8 0.5131200751823854 1104 9 0.6185264912973665 1104 10-11 0.7033608582222257 1104 12-13 0.7061087761657063 1104 14-15 0.7691579313808177 1104 16-17 0.6589390972022926 1104 18-19 0.7186943744016787 1104 20-21 0.6451334613481379 1104 22-23 0.653417226750129 1104 24-25 0.7862662656848016 1104 26-27 0.9082191297869926 1104 28-29 0.9032951465304677 1104 30-31 0.93380751007318 1104 32-33 0.9752141691846106 1104 34-35 0.9383541674681837 1104 36-37 0.887670174162352 1104 38-39 0.8973416029591164 1104 40-41 0.7753334720582643 1104 42-43 1.0256010058951617 1104 44-45 0.809607301913374 1104 46-47 1.1013984217649195 1104 48-49 1.0276373330252184 1104 50-51 0.7849437387909504 1104 52-53 0.9974952309179486 1104 54-55 1.1756384669901223 1104 56-57 1.135989011238813 1104 58-59 1.2285871886474808 1104 60-61 1.2631624629682392 1104 62-63 1.2415915137018239 1104 64-65 1.148985700935878 1104 66-67 1.3101557419942829 1104 68-69 1.5069079238719318 1104 70-71 1.1979088664934672 1104 72-73 1.8686148304029189 1104 74-75 1.8803755767607662 1104 76-77 1.9567968518925056 1104 78-79 1.930903840099809 1104 80-81 2.1011941110207317 1104 82-83 2.738683165637596 1104 84-85 2.4846540057457354 1104 86-87 2.587833258655735 1104 88-89 2.7776469533308976 1104 90-91 2.8518303975875234 1104 92-93 2.747596219482819 1104 94-95 2.703616876394662 1104 96-97 3.218948280063728 1104 98-99 3.398730642772856 1104 100-101 2.644423347499499 1105 1 0.7496241601816713 1105 2 0.6697198793321952 1105 3 0.6085381607311824 1105 4 0.49070089460525423 1105 5 0.5924674561807777 1105 6 0.6749057995040033 1105 7 0.5834035662263517 1105 8 0.45567362258100985 1105 9 0.5360630179589236 1105 10-11 0.7212504786091856 1105 12-13 0.5695198916925648 1105 14-15 0.702203838892423 1105 16-17 0.7755644417457006 1105 18-19 0.7177695543846596 1105 20-21 0.695630978291625 1105 22-23 0.7297606929712899 1105 24-25 0.8525874744288231 1105 26-27 0.6675720114856816 1105 28-29 0.568436250838019 1105 30-31 0.4831138417873646 1105 32-33 0.6578773098861674 1105 34-35 0.7236744609813428 1105 36-37 0.9002207702344265 1105 38-39 0.6995174871051617 1105 40-41 0.827718985626376 1105 42-43 0.7619724298090063 1105 44-45 0.5901665836222847 1105 46-47 1.0756387748160279 1105 48-49 1.0706185564086752 1105 50-51 1.1773625586652017 1105 52-53 1.2709510851458603 1105 54-55 1.14796844873878 1105 56-57 1.189446378421124 1105 58-59 1.0550039053020228 1105 60-61 1.2478214249323543 1105 62-63 1.416692546026841 1105 64-65 1.5332889055224719 1105 66-67 1.179456192364583 1105 68-69 1.5578657199022388 1105 70-71 1.8766303347837514 1105 72-73 2.057234962711849 1105 74-75 1.9580194379482663 1105 76-77 2.0105855773988957 1105 78-79 2.029206987151518 1105 80-81 2.444112152575837 1105 82-83 2.9841493633594283 1105 84-85 2.9121211602514094 1105 86-87 3.13484671862264 1105 88-89 2.867489275779839 1105 90-91 2.5625737589673356 1105 92-93 3.0224307800494756 1105 94-95 3.299920153432595 1105 96-97 3.253633292545601 1105 98-99 3.284967658800584 1105 100-101 2.576504480212236 1106 1 0.17372960345852562 1106 2 0.21234973896221732 1106 3 0.04690063376451192 1106 4 0.2231464597898949 1106 5 0.07605820952833398 1106 6 -0.12990460482212995 1106 7 0.09016723786948688 1106 8 -0.048842655670036095 1106 9 0.1697992992392443 1106 10-11 0.10352585184432428 1106 12-13 0.22378666424734917 1106 14-15 0.386643095819835 1106 16-17 0.009606004195802598 1106 18-19 0.36222496061431286 1106 20-21 0.32232829247231365 1106 22-23 -0.015063445425560928 1106 24-25 0.28238406281197115 1106 26-27 0.1678345144023794 1106 28-29 0.25513364689095397 1106 30-31 -0.0460812925445353 1106 32-33 0.3593234809255037 1106 34-35 0.2528680602084634 1106 36-37 0.17941078755040962 1106 38-39 0.12203102672928878 1106 40-41 0.21368270393669064 1106 42-43 -0.12373901961645828 1106 44-45 0.5013950563363139 1106 46-47 0.5541672643138611 1106 48-49 0.5497104293593083 1106 50-51 0.24235735977703143 1106 52-53 0.199986773261692 1106 54-55 0.17671993779041628 1106 56-57 0.3733094593559443 1106 58-59 0.6442809937608942 1106 60-61 0.9977723015795164 1106 62-63 0.9607418658730857 1106 64-65 0.8825625962827779 1106 66-67 1.0703603895457228 1106 68-69 1.0724062323406756 1106 70-71 0.8275259149100798 1106 72-73 1.1183305976925872 1106 74-75 1.465555267293741 1106 76-77 1.1544356406452074 1106 78-79 1.716661701560625 1106 80-81 1.6599540245030653 1106 82-83 1.7729158759698045 1106 84-85 1.742848226809457 1106 86-87 1.3625892344751556 1106 88-89 1.4693931127230542 1106 90-91 1.88518267976821 1106 92-93 1.7228402436633985 1106 94-95 1.6839562918458277 1106 96-97 1.831899586147692 1106 98-99 2.246922645434937 1106 100-101 1.5823358315547367 1107 1 0.18852432764881044 1107 2 0.08169200237314911 1107 3 0.17536011630499715 1107 4 0.055185617178558743 1107 5 0.1182797112995857 1107 6 0.1576633987203806 1107 7 0.1714037953087555 1107 8 0.2449049721133676 1107 9 0.23469523800442715 1107 10-11 0.16843562853966887 1107 12-13 0.12221032631718742 1107 14-15 0.32485080710526404 1107 16-17 0.40675855353791235 1107 18-19 0.5202422619807123 1107 20-21 0.5850908177759564 1107 22-23 0.3708922415683631 1107 24-25 0.40447612063079674 1107 26-27 0.3867347547869926 1107 28-29 0.5562331692846811 1107 30-31 0.7370862492064774 1107 32-33 0.43521266678076387 1107 34-35 0.5034452995809389 1107 36-37 0.43792506829433364 1107 38-39 0.7107301822201819 1107 40-41 0.6337488887798131 1107 42-43 0.5187125871649201 1107 44-45 0.6055175734162717 1107 46-47 0.6527724009535376 1107 48-49 0.32794573743117184 1107 50-51 0.4937490601818908 1107 52-53 0.42763653920298594 1107 54-55 0.6980402344756342 1107 56-57 0.7392653835715279 1107 58-59 0.8126194141809364 1107 60-61 0.8549490475309369 1107 62-63 0.7338551629378607 1107 64-65 0.6310582155894586 1107 66-67 0.7786093773999738 1107 68-69 0.8676206807212452 1107 70-71 1.1920901387208112 1107 72-73 0.9364468204911702 1107 74-75 0.9203348883716735 1107 76-77 1.260746527537922 1107 78-79 1.6706288384533394 1107 80-81 1.9677333134706778 1107 82-83 1.8542216230598854 1107 84-85 1.6327833071485287 1107 86-87 1.6247527750875044 1107 88-89 2.1010491571309515 1107 90-91 1.9543545389534351 1107 92-93 1.8494236982433563 1107 94-95 1.7657700886575682 1107 96-97 2.065207088678058 1107 98-99 2.049710395308418 1107 100-101 1.155949666321943 1108 1 0.1642454229217165 1108 2 0.1196446857308473 1108 3 0.10388069074456752 1108 4 0.0547553966619887 1108 5 0.07380899675280261 1108 6 0.1960063263519629 1108 7 0.09192912121031327 1108 8 0.13510546248861033 1108 9 0.15997773678610372 1108 10-11 0.19643708291344808 1108 12-13 0.02236966019876263 1108 14-15 0.05711468076511039 1108 16-17 0.2695962576071125 1108 18-19 0.12302118193684919 1108 20-21 0.0026026964309266987 1108 22-23 0.15889441932177562 1108 24-25 0.30322676786520475 1108 26-27 0.3502269270579461 1108 28-29 0.14844598757171923 1108 30-31 0.22410539211583824 1108 32-33 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-0.279560638865604 2316 84-85 -0.430250311264885 2316 86-87 -0.3543318313133774 2316 88-89 -0.6152657838219255 2316 90-91 -0.770211765741319 2316 92-93 -0.6362511182318045 2316 94-95 -0.1774596220550606 2316 96-97 -0.17235058019380745 2316 98-99 -0.14466498552375562 2316 100-101 0.09460939096214638 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3681.0 3 846.0 4 781.0 5 796.0 6 931.0 7 1177.0 8 1569.0 9 2069.0 10 2695.0 11 4002.0 12 5391.0 13 6977.0 14 8486.0 15 10396.0 16 12698.0 17 15408.0 18 14073.0 19 12211.0 20 13027.0 21 14183.0 22 15642.0 23 17706.0 24 20248.0 25 22677.0 26 25704.0 27 29550.0 28 33724.0 29 38600.0 30 44649.0 31 51125.0 32 59042.0 33 68939.0 34 80960.0 35 95004.0 36 107119.0 37 92727.0 38 28018.0 39 452.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.56294455126702 20.62175949809022 15.042494541626933 22.772801409015823 2 15.195862093314737 21.60573375465603 40.954455819787974 22.24394833224126 3 18.180987411756643 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28.536422782123672 30.30476396159924 20.875064634630043 48-49 20.34688106586317 28.449615340887558 30.41561042667856 20.78789316657071 50-51 20.561065922468643 28.81099104065732 29.712116422139268 20.91582661473477 52-53 20.370357873491944 28.88346024490152 29.905258857551907 20.840923024054625 54-55 20.604860080490898 28.644398521824577 29.866089017555282 20.884652380129246 56-57 20.677155601790066 28.78409643632641 29.480313391067636 21.058434570815884 58-59 20.564646108630193 28.677365621565954 29.61306132788229 21.144926941921565 60-61 20.630079032404584 28.40702337230881 29.730511180035556 21.23238641525105 62-63 20.467717863863474 28.27206540782558 29.863344364415028 21.39687236389592 64-65 20.460846477078366 28.2724486167598 29.69511356351433 21.571591342647505 66-67 20.33512518011901 28.41213929480551 29.56090410401432 21.691831421061156 68-69 20.504803911441297 28.10131168661959 29.654997713411863 21.738886688527245 70-71 20.224116566363097 27.865374322736784 29.77388480308817 22.136624307811946 72-73 20.24584049489948 27.70224427174205 29.877941476486097 22.17397375687238 74-75 20.33347159199747 27.676771661922782 29.552763503450326 22.43699324262942 76-77 20.128733197852025 27.669747438962037 29.683695265680925 22.517824097505017 78-79 20.10582367634753 27.524745485389417 29.473290241132215 22.89614059713083 80-81 20.037676768830337 27.54899589178908 29.401584106196786 23.011743233183797 82-83 20.280376985311484 27.492673911858528 29.528820272694002 22.698128830135982 84-85 20.39811609392122 27.142169710390707 29.436266074481043 23.023448121207025 86-87 20.064012158780468 27.153363934453463 29.450576145764177 23.332047761001895 88-89 20.606812887765468 26.83732889779734 29.35334308279096 23.20251513164623 90-91 20.5112283217562 26.879075338500684 29.222503682374494 23.387192657368615 92-93 20.35028649799675 26.557206678274014 29.471865678692417 23.62064114503682 94-95 20.346937726583416 26.61041332345534 29.125875378406963 23.916773571554284 96-97 20.472483458406558 26.409286654328067 28.990891416022695 24.127338471242677 98-99 20.6903085411004 25.969083596178482 28.869741626158408 24.470866236562713 100-101 21.225277400853653 25.269449501377455 29.04000153830083 24.46527155946806 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 276.0 1 184.0 2 88.5 3 80.5 4 65.0 5 82.0 6 136.5 7 238.0 8 356.0 9 420.5 10 424.0 11 411.0 12 475.0 13 589.5 14 711.0 15 812.0 16 970.0 17 1159.5 18 1375.5 19 1709.0 20 2008.5 21 2355.0 22 2932.0 23 3638.5 24 4517.0 25 5743.0 26 7319.0 27 9231.0 28 11480.0 29 13655.0 30 16024.5 31 18727.0 32 21120.0 33 23844.0 34 26920.5 35 30012.5 36 32955.5 37 35209.0 38 36193.0 39 36273.5 40 36441.5 41 36710.5 42 36863.5 43 37101.0 44 37573.5 45 37830.5 46 37923.0 47 37966.5 48 37556.5 49 36272.0 50 34624.5 51 32383.0 52 29349.0 53 26552.5 54 23641.0 55 20320.0 56 16988.0 57 13756.0 58 10876.5 59 8483.0 60 6466.5 61 4808.0 62 3507.0 63 2480.0 64 1718.5 65 1225.0 66 863.5 67 612.0 68 468.0 69 344.0 70 252.5 71 175.5 72 136.0 73 115.5 74 85.5 75 64.0 76 50.0 77 34.0 78 27.0 79 19.0 80 9.5 81 7.0 82 6.5 83 4.0 84 1.5 85 2.5 86 1.5 87 0.5 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38908607335538986 2 0.029482509293738184 3 0.03332354043411957 4 0.04640380864190482 5 0.06301367303274323 6 0.050867709696942646 7 0.03539977348297437 8 0.03768362983671465 9 0.03415403365366149 10-11 0.03602264339763081 12-13 0.02725055876621927 14-15 0.025018608238700363 16-17 0.02211188197030364 18-19 0.025797195632020913 20-21 0.033946410348776004 22-23 0.03384259869633327 24-25 0.04131703767221055 26-27 0.03648979583362314 28-29 0.031766365647478464 30-31 0.034309751132325594 32-33 0.029586320946180928 34-35 0.03109158990660066 36-37 0.032700670519463126 38-39 0.015000783777975943 40-41 0.011678810899808259 42-43 0.013080268207785252 44-45 0.009083519588739758 46-47 0.018374662482364995 48-49 0.016350335259731563 50-51 0.010225447765609899 52-53 0.008253026369197837 54-55 0.007111098192327696 56-57 0.008045403064312357 58-59 0.004411995228816454 60-61 0.007318721497213176 62-63 0.00991401280828168 64-65 0.005761546710572075 66-67 0.0032181612257249424 68-69 0.005969170015457555 70-71 0.012820739076678401 72-73 0.01012163611316716 74-75 0.011678810899808259 76-77 0.005242488448358375 78-79 0.007993497238090987 80-81 0.009343048719846607 82-83 0.01199024585713648 84-85 0.007422533149655916 86-87 0.0029586320946180925 88-89 0.015831276997517865 90-91 0.02590100728446365 92-93 0.010381165244274008 94-95 0.02071042466232665 96-97 0.018738003265914585 98-99 0.01090022350648771 100-101 0.12275727901354015 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 963283.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.31846440119519 #Duplication Level Percentage of deduplicated Percentage of total 1 78.507289930602 55.20512072219347 2 13.695113395349706 19.26038687522459 3 4.266159786320091 8.999694151924793 4 1.7073931999529324 4.802450717989321 5 0.7843287056375894 2.7576395083106164 6 0.38215203278738363 1.6123406468042403 7 0.2136020386273833 1.0514117144469661 8 0.11617463134528949 0.653537734286057 9 0.08801954251212622 0.5570459160073591 >10 0.22335620568398112 2.488880600284594 >50 0.008576965964693786 0.42671920827402127 >100 0.007094550058414709 0.8608759148358458 >500 2.956060633674583E-4 0.16161553854962807 >1k 4.434090950511875E-4 1.1622807508684827 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4722 0.49019862283461874 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3969 0.41202844854523535 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2492 0.2586986378873083 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 980 0.10173541939388529 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 3.1143495732822025E-4 0.0 22-23 0.0 0.0 0.0 0.0010900223506487708 0.0 24-25 0.0 0.0 0.0 0.002387668006183022 0.0 26-27 0.0 0.0 0.0 0.006384416625228515 0.0 28-29 0.0 0.0 0.0 0.015260312909082793 0.0 30-31 0.0 0.0 0.0 0.04484663385526372 0.0 32-33 0.0 0.0 0.0 0.10692600201602229 0.0 34-35 0.0 0.0 0.0 0.2146305914253651 0.0 36-37 0.0 0.0 0.0 0.38259784507771855 0.0 38-39 0.0 0.0 0.0 0.5916226072711758 0.0 40-41 0.0 0.0 0.0 0.7936919887509694 0.0 42-43 0.0 0.0 0.0 0.9976818858009536 0.0 44-45 0.0 0.0 0.0 1.2749628094755123 0.0 46-47 0.0 0.0 0.0 1.5899273629867858 0.0 48-49 0.0 0.0 0.0 1.9089924767695474 0.0 50-51 0.0 0.0 0.0 2.2151849456494093 0.0 52-53 0.0 0.0 0.0 2.513228199812516 0.0 54-55 0.0 0.0 0.0 2.859076719925505 0.0 56-57 0.0 0.0 0.0 3.265603150891275 0.0 58-59 0.0 0.0 0.0 3.7074255436875765 0.0 60-61 0.0 0.0 0.0 4.146548833520367 0.0 62-63 0.0 0.0 0.0 4.592835127371707 0.0 64-65 0.0 0.0 0.0 5.070991598522967 0.0 66-67 0.0 0.0 0.0 5.609203110612354 0.0 68-69 0.0 0.0 0.0 6.204718654850133 0.0 70-71 0.0 0.0 0.0 6.808798660414437 0.0 72-73 0.0 0.0 0.0 7.4095047872743525 0.0 74-75 0.0 0.0 0.0 8.028170330006862 0.0 76-77 0.0 0.0 0.0 8.710939568122765 0.0 78-79 0.0 0.0 0.0 9.463210707549079 0.0 80-81 0.0 0.0 0.0 10.211744627487457 0.0 82-83 0.0 0.0 0.0 10.969673501971902 0.0 84-85 0.0 0.0 0.0 11.721166054004897 0.0 86-87 0.0 0.0 0.0 12.51454660779854 0.0 88-89 0.0 0.0 0.0 13.335073908705956 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2445 0.0 48.56908 1 GTATCAA 4145 0.0 37.163563 1 TATCAAC 4975 0.0 30.86042 2 TCAACGC 5060 0.0 30.248075 4 ATCAACG 5075 0.0 30.065012 3 CAACGCA 5210 0.0 29.37721 5 AACGCAG 5235 0.0 29.318571 6 CGACCCG 35 0.008362711 27.162949 94-95 TGACGCG 80 6.2016625E-8 26.73853 94-95 CGCGACT 45 8.9626695E-4 26.382372 92-93 ACGCAGA 5870 0.0 26.059252 7 TAGCCCT 1095 0.0 25.611221 4 CGCAGAG 5975 0.0 25.601309 8 GTGGTAT 1180 0.0 25.058151 1 GTAGCCC 645 0.0 25.05599 3 GCAGAGT 6250 0.0 23.865541 9 CGACGAG 50 0.0016469413 23.76758 94-95 GCGCCGT 60 1.7028362E-4 23.76758 94-95 GTACATG 3315 0.0 22.87457 1 GTATAGC 775 0.0 22.76876 1 >>END_MODULE