FastQCFastQC Report
Thu 26 May 2016
SRR938508_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938508_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences963283
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43900.45573315422362903No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40270.4180495243869143No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26170.2716750944426508No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT12760.13246366851693633No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9900.10277353591831269No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25100.043.155571
GTATCAA38650.032.9428371
TCGTATC902.1100277E-1029.02723994-95
AACTGCG700.00839166627.1423537
TCAACGC49150.025.8998264
TATCAAC48850.025.8670982
ATCAACG49150.025.8031853
CAACGCA49700.025.6132075
AACGCAG49850.025.3455666
CTCCGAC2850.024.99953794-95
GTGGTAT13750.024.531911
CCTATAC2354.647518E-922.233633
ACGCAGA57650.021.833947
CGCAGAG58100.021.5013228
TGGTATC14250.021.3351542
GTACATG35350.021.2345661
TACATGG36400.020.2283732
GAGTACT42100.020.0263512-13
CATGGGT14250.019.9996284
ACATGGG35600.019.7468243
Produced by FastQC (version 0.11.5)