##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938508_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 963283 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.78344785488792 31.0 31.0 34.0 30.0 34.0 2 32.03955431581373 31.0 31.0 34.0 30.0 34.0 3 32.08957492242674 33.0 31.0 34.0 30.0 34.0 4 35.75445948905981 37.0 35.0 37.0 35.0 37.0 5 35.64897750712927 37.0 35.0 37.0 33.0 37.0 6 35.67653119592062 37.0 35.0 37.0 33.0 37.0 7 35.59905967405217 37.0 35.0 37.0 33.0 37.0 8 35.581303729018366 37.0 35.0 37.0 33.0 37.0 9 37.11997305049503 39.0 37.0 39.0 33.0 39.0 10-11 37.06773762227715 39.0 37.0 39.0 33.0 39.0 12-13 36.97127583482735 39.0 37.0 39.0 33.0 39.0 14-15 37.992686988143674 40.0 37.0 41.0 33.0 41.0 16-17 38.052307058258066 40.0 37.0 41.0 33.0 41.0 18-19 38.21889621222424 40.0 37.5 41.0 33.0 41.0 20-21 38.20514272545036 40.0 37.5 41.0 33.0 41.0 22-23 38.080333090068024 40.0 37.0 41.0 33.0 41.0 24-25 37.98995414639312 40.0 37.0 41.0 33.0 41.0 26-27 37.79534467025786 40.0 37.0 41.0 32.0 41.0 28-29 37.66160982805676 40.0 37.0 41.0 32.0 41.0 30-31 37.417650368583274 39.0 36.0 41.0 32.0 41.0 32-33 37.27888585182132 39.0 36.0 41.0 31.5 41.0 34-35 37.13391910788418 39.0 36.0 41.0 31.0 41.0 36-37 37.01484714253236 39.0 36.0 41.0 31.0 41.0 38-39 36.81481298849871 39.0 35.0 41.0 30.5 41.0 40-41 36.60306680383646 39.0 35.0 40.5 30.0 41.0 42-43 36.350012405492464 39.0 35.0 40.0 30.0 41.0 44-45 36.02116667687481 39.0 35.0 40.0 29.5 41.0 46-47 35.55913578875574 38.0 35.0 40.0 27.5 41.0 48-49 35.14369764648603 38.0 34.5 40.0 26.5 41.0 50-51 34.76266060960279 38.0 34.0 40.0 24.5 41.0 52-53 34.242003128883205 38.0 33.0 40.0 23.5 41.0 54-55 33.87714254274185 38.0 33.0 40.0 23.0 41.0 56-57 33.54968840932519 38.0 33.0 40.0 21.5 41.0 58-59 33.100489160506314 37.0 32.0 40.0 19.0 41.0 60-61 32.71073090670136 37.0 31.0 39.0 18.0 40.0 62-63 32.30098890980117 36.0 31.0 39.0 13.5 40.0 64-65 31.743170490914924 36.0 30.5 39.0 8.5 40.0 66-67 31.17790566219896 35.0 29.5 38.5 7.5 40.0 68-69 30.53496272642619 35.0 28.5 38.0 6.0 40.0 70-71 29.86588520715096 34.0 28.0 37.5 2.0 39.5 72-73 29.349460127501473 34.0 27.5 37.0 2.0 39.0 74-75 28.703663928461317 33.5 26.0 36.0 2.0 39.0 76-77 27.484129793632817 31.5 26.0 34.5 2.0 37.0 78-79 28.042161026406568 33.0 26.0 35.0 2.0 37.5 80-81 27.692549333892533 33.0 26.0 35.0 2.0 37.0 82-83 27.076613518561004 32.5 25.5 35.0 2.0 36.5 84-85 26.361417153629827 32.0 24.5 35.0 2.0 36.0 86-87 25.533357798279425 31.0 21.0 34.0 2.0 35.5 88-89 24.653019932875388 31.0 18.5 34.0 2.0 35.0 90-91 23.87768547768413 30.5 11.0 34.0 2.0 35.0 92-93 22.893105660537973 30.0 4.0 34.0 2.0 35.0 94-95 21.808060040507307 29.0 2.0 34.0 2.0 35.0 96-97 20.492449259459576 28.5 2.0 33.5 2.0 34.5 98-99 18.71716878632759 26.0 2.0 33.0 2.0 34.0 100-101 14.194650481737973 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3686742044534803 1101 2 0.2778756353137979 1101 3 0.3974412779985457 1101 4 0.22500226321597694 1101 5 0.32256056261272903 1101 6 0.3237780067614153 1101 7 0.33457038622710655 1101 8 0.3422353597411174 1101 9 0.49696408314405005 1101 10-11 0.46648305792890454 1101 12-13 0.4157539910901136 1101 14-15 0.5314596888714576 1101 16-17 0.548546981118669 1101 18-19 0.49343903493026886 1101 20-21 0.5119232756140164 1101 22-23 0.43175381863644446 1101 24-25 0.5535177334956387 1101 26-27 0.6089286545213781 1101 28-29 0.6805538187085673 1101 30-31 0.8118466004119327 1101 32-33 0.673803470991011 1101 34-35 0.2964061135933349 1101 36-37 0.4123280595121983 1101 38-39 0.7330174993222585 1101 40-41 0.7748762344298328 1101 42-43 0.8186231315939594 1101 44-45 0.9060034881259114 1101 46-47 0.7986557967050487 1101 48-49 1.1007633158947954 1101 50-51 1.1103420716174455 1101 52-53 1.1802123637036317 1101 54-55 1.2603237420399083 1101 56-57 1.250053570101457 1101 58-59 1.2721734232767972 1101 60-61 1.5179932331461004 1101 62-63 1.4409296724277212 1101 64-65 1.5192781908324768 1101 66-67 1.5940282380568611 1101 68-69 1.526205973856932 1101 70-71 1.5655975634641308 1101 72-73 1.8484247477909008 1101 74-75 1.9297513768380945 1101 76-77 2.042696748993098 1101 78-79 1.8653915185055006 1101 80-81 1.858120241476378 1101 82-83 1.911367556089278 1101 84-85 1.9234325544204331 1101 86-87 2.0828692042785626 1101 88-89 1.98843900088616 1101 90-91 2.3282021168574474 1101 92-93 2.645896252127873 1101 94-95 2.6682431476532145 1101 96-97 2.6869751453095354 1101 98-99 2.2897850099467263 1101 100-101 2.2477104705795004 1102 1 0.18361932341349885 1102 2 0.2140725938617507 1102 3 0.09264455246506742 1102 4 0.1703511717271411 1102 5 0.33477849246310853 1102 6 0.33029321402165124 1102 7 0.2738256597125144 1102 8 0.25631893835284103 1102 9 0.41132359994561796 1102 10-11 0.29713365886096454 1102 12-13 0.3412468473569774 1102 14-15 0.3600484183293915 1102 16-17 0.35464291562934136 1102 18-19 0.3028500601939399 1102 20-21 0.24061088292331334 1102 22-23 0.2752815350946207 1102 24-25 0.3958420528480957 1102 26-27 0.5672581553798537 1102 28-29 0.34158674733255623 1102 30-31 0.23583329405921916 1102 32-33 0.1999524469537235 1102 34-35 0.35219161764042894 1102 36-37 0.26036276640451916 1102 38-39 0.395468443644063 1102 40-41 0.4644802631274061 1102 42-43 0.2607872534973126 1102 44-45 0.33361953546464207 1102 46-47 0.3205622229988947 1102 48-49 0.6468143338001227 1102 50-51 0.6305852038224984 1102 52-53 0.4697520068235761 1102 54-55 0.5174828050764475 1102 56-57 0.6247239772635851 1102 58-59 0.5844396423104001 1102 60-61 0.7558634502174826 1102 62-63 0.8388172122854627 1102 64-65 1.1315007196526885 1102 66-67 1.2025562611130347 1102 68-69 1.3252961443254136 1102 70-71 1.2685462696206145 1102 72-73 1.4497860428608078 1102 74-75 1.3591341950868063 1102 76-77 1.2152271021939782 1102 78-79 1.4012289909116795 1102 80-81 1.5084758951432882 1102 82-83 1.569116053759135 1102 84-85 1.7916644038194995 1102 86-87 1.7564113308422087 1102 88-89 1.5477043921051923 1102 90-91 1.5020546738846718 1102 92-93 1.8320817132512488 1102 94-95 1.4174843176311427 1102 96-97 1.814887826202348 1102 98-99 1.8548642655268104 1102 100-101 1.8098157256690275 1103 1 0.31445721572562135 1103 2 0.2198241055231378 1103 3 0.18744731664588699 1103 4 0.2333154677248359 1103 5 0.1654148299235203 1103 6 0.18559362672920798 1103 7 0.24104180329635483 1103 8 0.3533364064399862 1103 9 0.10299266607480462 1103 10-11 0.3385039919063644 1103 12-13 0.4222706980144224 1103 14-15 0.36472257131912045 1103 16-17 0.3877830922297818 1103 18-19 0.2723540912909712 1103 20-21 0.40875801796506295 1103 22-23 0.4195960086525474 1103 24-25 0.2562653180366965 1103 26-27 0.5900830426051158 1103 28-29 0.2285912583220906 1103 30-31 0.3203711575777888 1103 32-33 0.23639908290727618 1103 34-35 0.5216476594870585 1103 36-37 0.5191316021853112 1103 38-39 0.2126270001274122 1103 40-41 0.39593601703852954 1103 42-43 0.4886966018032908 1103 44-45 0.535416732892422 1103 46-47 0.6955358289111686 1103 48-49 0.6480056669414935 1103 50-51 0.4702555176722072 1103 52-53 0.6366586197422777 1103 54-55 0.48023114944731304 1103 56-57 0.7258889163172384 1103 58-59 0.821866458703532 1103 60-61 0.7926460109238818 1103 62-63 0.9590254856322318 1103 64-65 1.2376457431674233 1103 66-67 1.024392569779888 1103 68-69 0.7053374150807628 1103 70-71 0.9145055747362711 1103 72-73 0.9289803033464565 1103 74-75 0.7977163526835014 1103 76-77 1.1493090678336735 1103 78-79 1.1584621706794138 1103 80-81 1.494311867885397 1103 82-83 1.505273554478972 1103 84-85 1.5623717653705143 1103 86-87 1.4303027791577883 1103 88-89 1.3910758768926001 1103 90-91 1.9093766337656604 1103 92-93 2.309387596733348 1103 94-95 2.217427930261909 1103 96-97 2.2360894770325643 1103 98-99 2.239910815099705 1103 100-101 1.8330617185666203 1104 1 0.2680651325259156 1104 2 0.2726703509989292 1104 3 0.33864766483849706 1104 4 0.22709267027214253 1104 5 0.10987810934808095 1104 6 0.08858647961783106 1104 7 0.13646833923807833 1104 8 0.1814289065545296 1104 9 0.37281902792022237 1104 10-11 0.3859572607935249 1104 12-13 0.3399901509855212 1104 14-15 0.4601846137436425 1104 16-17 0.44319668548584445 1104 18-19 0.3115870367255269 1104 20-21 0.46151263380782126 1104 22-23 0.3415980015970561 1104 24-25 0.3150686350294123 1104 26-27 0.47621890644347786 1104 28-29 0.2789944287073922 1104 30-31 0.27815738564394366 1104 32-33 0.5793991516295023 1104 34-35 0.5821089001522921 1104 36-37 0.5216073203325564 1104 38-39 0.36241052511938676 1104 40-41 0.639618726526777 1104 42-43 0.5652124995446215 1104 44-45 0.7430804063244025 1104 46-47 0.7352435303818297 1104 48-49 0.9550055982192731 1104 50-51 0.9009342641788223 1104 52-53 0.9696636135572803 1104 54-55 1.2337875245790286 1104 56-57 1.2922751581278433 1104 58-59 1.1029632654108141 1104 60-61 1.0109152416931053 1104 62-63 1.2304278771656598 1104 64-65 1.555713961429202 1104 66-67 1.3694119265398648 1104 68-69 1.4881316607398105 1104 70-71 1.5416645979643846 1104 72-73 1.7983427901747007 1104 74-75 1.692553595547846 1104 76-77 1.8973573566502786 1104 78-79 1.7910863288321615 1104 80-81 1.968070286357932 1104 82-83 2.1877665677194535 1104 84-85 2.2036532057883456 1104 86-87 1.8382001081539876 1104 88-89 1.8037465370841055 1104 90-91 2.305844884097816 1104 92-93 2.697294775915097 1104 94-95 1.8986501550294008 1104 96-97 2.4189249560264656 1104 98-99 1.9925612022349064 1104 100-101 1.5070618101962534 1105 1 0.2030315332180379 1105 2 0.30104250794983045 1105 3 0.12450495061960254 1105 4 0.07277451726982775 1105 5 0.15287691283555205 1105 6 0.035315568084108406 1105 7 0.05431278408502749 1105 8 0.09795217994858518 1105 9 0.23898862158542045 1105 10-11 0.2680666814918098 1105 12-13 0.2462722146979459 1105 14-15 0.442123986890401 1105 16-17 0.25086448757861746 1105 18-19 0.30254873234254376 1105 20-21 0.3463970472876099 1105 22-23 0.44058943635729975 1105 24-25 0.33513286100939865 1105 26-27 0.40536362905607604 1105 28-29 0.40912715316537174 1105 30-31 0.6451436550499849 1105 32-33 0.68244306672932 1105 34-35 0.6119535239966609 1105 36-37 0.6240481845917785 1105 38-39 0.8094672427967708 1105 40-41 0.7721114467655283 1105 42-43 0.7731126786485945 1105 44-45 0.778313598413142 1105 46-47 0.8486197520658649 1105 48-49 0.7139434829172302 1105 50-51 0.7619390363779601 1105 52-53 1.0097627653439005 1105 54-55 1.04102488048877 1105 56-57 1.2097994319896301 1105 58-59 1.2308654475811878 1105 60-61 1.2078507631988984 1105 62-63 1.2833935341661018 1105 64-65 1.4492989282028113 1105 66-67 1.4396226001135624 1105 68-69 1.5722231582556887 1105 70-71 1.5464570408636718 1105 72-73 1.7217255005153156 1105 74-75 1.5811213071829968 1105 76-77 1.3419784308265967 1105 78-79 1.425411109000951 1105 80-81 1.746662727339391 1105 82-83 2.1047932713647164 1105 84-85 2.2471543740661666 1105 86-87 2.0184979257705287 1105 88-89 1.9805981215842081 1105 90-91 1.749606664099332 1105 92-93 1.7896201548728818 1105 94-95 1.8319501749535192 1105 96-97 2.0058063627757363 1105 98-99 2.0833890759692686 1105 100-101 1.999694681155093 1106 1 -0.1542630799969622 1106 2 -0.027548716163181552 1106 3 0.003280796667013419 1106 4 -0.056945929916423665 1106 5 0.049440529624277474 1106 6 0.04615954822190105 1106 7 -0.04320832872265612 1106 8 -0.05115664055878 1106 9 0.009611219146442807 1106 10-11 0.022479084319677156 1106 12-13 0.030911342267202713 1106 14-15 -0.11308503297589567 1106 16-17 -0.15821893206171467 1106 18-19 -0.058241314447450065 1106 20-21 -0.09635991901786412 1106 22-23 0.23921183333129648 1106 24-25 0.04450682481367352 1106 26-27 -0.24166972979411128 1106 28-29 0.12177907736803206 1106 30-31 0.045010305615100776 1106 32-33 -0.004823853195524919 1106 34-35 -0.014294516186240003 1106 36-37 -0.18602814081768315 1106 38-39 -0.10334289953814846 1106 40-41 -0.06299066840417566 1106 42-43 0.05362135096716969 1106 44-45 0.09493970420029285 1106 46-47 0.00694227143889492 1106 48-49 0.12171848158679666 1106 50-51 0.14408494911032932 1106 52-53 -0.31788283422709185 1106 54-55 -0.22978909346766585 1106 56-57 -0.10822259574049298 1106 58-59 -0.019776737916778586 1106 60-61 0.21435653723967363 1106 62-63 0.22224058860705753 1106 64-65 0.4788532770523055 1106 66-67 0.43978378611504354 1106 68-69 0.4903708598968173 1106 70-71 0.3029715138315012 1106 72-73 0.4196685624557688 1106 74-75 0.5631999823261715 1106 76-77 0.6252288002751492 1106 78-79 0.7877725913799942 1106 80-81 0.9556611021751351 1106 82-83 1.0635291432837626 1106 84-85 1.2887843687854037 1106 86-87 1.0236446203829246 1106 88-89 0.9928999395576241 1106 90-91 0.9795249351210558 1106 92-93 0.9431976653832344 1106 94-95 0.7657886942119205 1106 96-97 0.8453572115863395 1106 98-99 1.2324605422604158 1106 100-101 1.4107901114551975 1107 1 0.13491407713866366 1107 2 0.0790547441100955 1107 3 0.09025811827632424 1107 4 0.11841227479005312 1107 5 0.21130477557569094 1107 6 0.02556475444659867 1107 7 0.1014613005789613 1107 8 0.11473246622259836 1107 9 0.1851512937921953 1107 10-11 0.14152284380853786 1107 12-13 0.052111051275296916 1107 14-15 0.2552371772973814 1107 16-17 0.11620106097978322 1107 18-19 -0.01764675789380732 1107 20-21 0.17113913209549736 1107 22-23 0.18291122604384924 1107 24-25 0.06008240363452444 1107 26-27 0.24658779803989717 1107 28-29 0.10101822843078878 1107 30-31 0.2243538342082232 1107 32-33 0.19044120247249197 1107 34-35 0.07353284970444918 1107 36-37 0.17574675164183162 1107 38-39 0.27233318219262515 1107 40-41 0.4360090469298328 1107 42-43 0.4171655216402499 1107 44-45 0.2671507682185066 1107 46-47 0.5045838927698654 1107 48-49 0.5627135473762763 1107 50-51 0.3140819443026359 1107 52-53 0.5634588914814103 1107 54-55 0.47925458694731304 1107 56-57 0.6567737668607165 1107 58-59 0.8843876883230948 1107 60-61 0.5477241359238789 1107 62-63 0.9266248402517974 1107 64-65 1.128508514906553 1107 66-67 1.1631833306494563 1107 68-69 1.063387686819894 1107 70-71 1.0671889986493142 1107 72-73 1.462085772096458 1107 74-75 1.6682496407269802 1107 76-77 1.4217657594097624 1107 78-79 1.6588485497555006 1107 80-81 1.4613465146245268 1107 82-83 1.4587127528485375 1107 84-85 1.6216024039574712 1107 86-87 1.9384464612230055 1107 88-89 2.089097276349122 1107 90-91 2.263694738385226 1107 92-93 2.318711645646392 1107 94-95 2.3005673664032145 1107 96-97 2.235134144152127 1107 98-99 2.4384756928170965 1107 100-101 2.3857493884035765 1108 1 0.07170783018447224 1108 2 0.017398052467797243 1108 3 -0.04657675319053567 1108 4 -0.06459325335322319 1108 5 0.0029942858096063674 1108 6 -0.017718094603111467 1108 7 -0.013931075761192346 1108 8 -0.06791327573646555 1108 9 0.09778035994716561 1108 10-11 0.17323257059947395 1108 12-13 -5.776365902008251E-4 1108 14-15 0.09974672590849565 1108 16-17 0.0061797783896295755 1108 18-19 -0.10433143290598679 1108 20-21 -0.014788469025361906 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-0.23919212665561496 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 6.0 4 12.0 5 27.0 6 79.0 7 193.0 8 450.0 9 829.0 10 1443.0 11 2245.0 12 3305.0 13 4603.0 14 6012.0 15 7970.0 16 10436.0 17 13381.0 18 12624.0 19 10142.0 20 9712.0 21 10152.0 22 11219.0 23 12581.0 24 14210.0 25 16544.0 26 19161.0 27 22575.0 28 27190.0 29 32168.0 30 38120.0 31 44847.0 32 54423.0 33 65441.0 34 81242.0 35 102860.0 36 130700.0 37 139528.0 38 55573.0 39 1275.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.2890875673655 21.38053850866536 15.595568154678464 22.734805769290674 2 15.30860044581907 22.305385025089002 40.17157908616434 22.21443544292758 3 18.1303080856789 25.68477614295458 31.773716846038134 24.411198925328385 4 12.31112767483699 19.18024090532066 38.210577784514 30.298053635328355 5 12.44412332006619 38.38294497353838 35.80218461468189 13.37074709171354 6 29.603034622224207 38.44041678302223 18.307911589844313 13.648637004909252 7 25.974713557698 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20.221098516424597 30-31 20.282045878521682 28.578154083483255 31.335806818972202 19.80399321902286 32-33 19.74172605147312 28.711760190059067 31.298548344483613 20.247965413984208 34-35 20.155327286771485 29.01176504788246 31.14285625295891 19.690051412387145 36-37 20.146023895763065 28.744885893526924 30.827537951575213 20.2815522591348 38-39 19.88030206364475 28.86226742921454 31.063945318319785 20.193485188820926 40-41 20.574935149005444 28.455998658595398 30.98649822279464 19.98256796960452 42-43 20.25681328116159 28.46228674593477 30.880299492644014 20.400600480259627 44-45 20.42143969055389 28.738063439151762 30.611370961086177 20.22912590920817 46-47 20.41474895967586 28.58977360200523 30.164027856862198 20.831449581456717 48-49 20.432988021173422 28.54005520703677 30.270128300821252 20.756828470968554 50-51 20.520553150008876 28.836593192239455 29.775881023541366 20.8669726342103 52-53 20.353779730359616 28.974299349204752 29.813668465030524 20.85825245540511 54-55 20.58439731626116 28.67350508625191 29.605837536840163 21.136260060646766 56-57 20.63780841144295 28.939885786419982 29.162198440126108 21.260107362010956 58-59 20.41949250635587 28.932255629965443 29.42048183140365 21.227770032275043 60-61 20.51344205181655 28.795276154567244 29.35004562522125 21.341236168394957 62-63 20.303327267272444 28.787957433070034 29.379735757819876 21.528979541837653 64-65 20.258501664102514 28.693310993042537 29.27237299150197 21.775814351352977 66-67 20.17652225006696 28.85475428692444 29.121136369770724 21.84758709323788 68-69 20.336955184586724 28.527360090358556 29.099990812578152 22.03569391247657 70-71 20.078429744130098 28.290195243866673 29.24827348155915 22.38310153044408 72-73 19.994300734364394 28.135322781906027 29.298170213914513 22.57220626981507 74-75 20.093939164295435 28.046949857933757 28.965786793704446 22.893324184066365 76-77 19.70319210450096 28.062833040757496 29.466522299262003 22.767452555479544 78-79 19.670750963112603 27.906492692178723 29.257445631242323 23.165310713466344 80-81 19.46688508661015 28.00021177599083 29.201573371037764 23.331329766361254 82-83 19.554602556176874 27.904091720480505 29.385393260176617 23.155912463166004 84-85 19.590762118477475 27.713800293699475 29.21515588964001 23.48028169818304 86-87 19.217500807165763 27.743039947178655 29.230716524422085 23.808742721233493 88-89 19.603325294851047 27.458337788583414 29.038558762066806 23.89977815449873 90-91 19.43614802541767 27.379895709081698 29.141452992783933 24.042503272716704 92-93 19.128580156206123 27.210303147013036 29.3171185695518 24.343998127229042 94-95 18.97152757808453 27.260939931463547 29.15337444966848 24.614158040783447 96-97 18.988396971606473 27.06779835209383 29.092592727163254 24.85121194913644 98-99 19.083280821939137 26.79093267502904 29.020910781151542 25.104875721880276 100-101 18.84157494897652 26.81665389782016 29.23551981662691 25.106251336576413 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 344.0 1 211.5 2 92.5 3 104.5 4 80.0 5 73.5 6 128.5 7 234.5 8 341.5 9 411.5 10 417.0 11 407.0 12 489.0 13 565.0 14 656.0 15 786.0 16 921.0 17 1124.5 18 1329.0 19 1630.0 20 1976.5 21 2301.0 22 2797.5 23 3544.0 24 4504.5 25 5674.5 26 7242.0 27 9133.5 28 11324.0 29 13617.0 30 15908.5 31 18547.0 32 21449.5 33 24226.0 34 27075.5 35 30212.0 36 33342.0 37 35522.0 38 35947.5 39 35959.0 40 36547.0 41 37146.0 42 37493.5 43 37757.0 44 38467.5 45 38975.5 46 38900.5 47 38884.0 48 38546.0 49 37300.0 50 35614.5 51 32953.0 52 29291.5 53 26024.0 54 22878.5 55 19360.5 56 15985.0 57 12962.0 58 10107.5 59 7777.5 60 5849.0 61 4186.5 62 2975.5 63 2124.5 64 1484.0 65 988.0 66 641.0 67 444.5 68 324.0 69 222.5 70 160.5 71 106.5 72 83.5 73 82.5 74 56.0 75 34.0 76 21.5 77 13.0 78 10.0 79 7.5 80 7.0 81 7.5 82 3.5 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026264348068013244 2 0.01162690507358689 3 0.001245739829312881 4 0.0 5 1.0381165244274008E-4 6 0.0 7 0.0 8 1.0381165244274008E-4 9 1.0381165244274008E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0016609864390838413 26-27 0.008460649674083318 28-29 2.0762330488548016E-4 30-31 0.0 32-33 3.633407835495903E-4 34-35 0.009446860372289348 36-37 0.0016609864390838413 38-39 0.0112635642900373 40-41 0.011782622552251 42-43 0.005605829231907965 44-45 0.0014014573079769912 46-47 1.5571747866411013E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 1.0381165244274008E-4 66-67 5.190582622137005E-4 68-69 9.86210698206031E-4 70-71 5.190582622137004E-5 72-73 1.0381165244274008E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.0381165244274008E-4 82-83 8.823990457632907E-4 84-85 0.005553923405686595 86-87 0.0037372194879386434 88-89 0.0 90-91 0.0016609864390838413 92-93 4.6715243599233043E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 1.0381165244274008E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 963283.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.62276366299757 #Duplication Level Percentage of deduplicated Percentage of total 1 77.24482029656855 53.00753047402142 2 14.319442423510202 19.652794264288833 3 4.555554773443047 9.378442757154682 4 1.8496717234710354 5.077183421355292 5 0.8416250905287135 2.887731984010043 6 0.440546037793865 1.8138891980519039 7 0.24256229001863538 1.1651706291053006 8 0.140139446181032 0.7693404876115463 9 0.09683885773253438 0.5980815042816917 >10 0.24879276764338812 2.6502814561839894 >50 0.010612456687174752 0.4927719220577549 >100 0.008181729145127619 0.9271248382228083 >500 6.060536383488925E-4 0.2997532623258505 >1k 6.060536383488925E-4 1.2799038013289004 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4390 0.45573315422362903 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4027 0.4180495243869143 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2617 0.2716750944426508 No Hit CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 1276 0.13246366851693633 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 990 0.10277353591831269 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 3.1143495732822025E-4 0.0 22-23 0.0 0.0 0.0 0.0010900223506487708 0.0 24-25 0.0 0.0 0.0 0.002387668006183022 0.0 26-27 0.0 0.0 0.0 0.006384416625228515 0.0 28-29 0.0 0.0 0.0 0.015000783777975943 0.0 30-31 0.0 0.0 0.0 0.044639010550378236 0.0 32-33 0.0 0.0 0.0 0.10640694375380857 0.0 34-35 0.0 0.0 0.0 0.2141634389893728 0.0 36-37 0.0 0.0 0.0 0.3827535625563827 0.0 38-39 0.0 0.0 0.0 0.5934393111889237 0.0 40-41 0.0 0.0 0.0 0.7974811140651293 0.0 42-43 0.0 5.1905826221370046E-5 0.0 1.0026129392919838 0.0 44-45 0.0 1.0381165244274009E-4 0.0 1.2807243561860844 0.0 46-47 0.0 1.0381165244274009E-4 0.0 1.596259873785793 0.0 48-49 0.0 1.0381165244274009E-4 0.0 1.916051669135654 0.0 50-51 0.0 1.0381165244274009E-4 0.0 2.220738869055096 0.0 52-53 0.0 1.0381165244274009E-4 0.0 2.5186264057395387 0.0 54-55 0.0 1.0381165244274009E-4 0.0 2.863021562718329 0.0 56-57 0.0 1.0381165244274009E-4 0.0 3.2699113344676487 0.0 58-59 0.0 1.0381165244274009E-4 0.0 3.7107994223919656 0.0 60-61 0.0 1.0381165244274009E-4 0.0 4.146964080130138 0.0 62-63 0.0 1.0381165244274009E-4 0.0 4.591900822499722 0.0 64-65 0.0 1.0381165244274009E-4 0.0 5.070264916955869 0.0 66-67 0.0 1.0381165244274009E-4 0.0 5.608787864002583 0.0 68-69 0.0 1.0381165244274009E-4 0.0 6.204199596587919 0.0 70-71 0.0 1.0381165244274009E-4 0.0 6.811705386682833 0.0 72-73 0.0 1.0381165244274009E-4 0.0 7.419055459299084 0.0 74-75 0.0 1.0381165244274009E-4 0.0 8.052669879983348 0.0 76-77 0.0 1.0381165244274009E-4 0.0 8.75360615727673 0.0 78-79 0.0 1.0381165244274009E-4 0.0 9.51963234065171 0.0 80-81 0.0 1.0381165244274009E-4 0.0 10.284724219154704 0.0 82-83 0.0 1.0381165244274009E-4 0.0 11.069125065012047 0.0 84-85 0.0 1.0381165244274009E-4 0.0 11.848439139899696 0.0 86-87 0.0 1.0381165244274009E-4 0.0 12.673170812731046 0.0 88-89 0.0 1.0381165244274009E-4 0.0 13.537350913490634 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2510 0.0 43.15557 1 GTATCAA 3865 0.0 32.942837 1 TCGTATC 90 2.1100277E-10 29.027239 94-95 AACTGCG 70 0.008391666 27.142353 7 TCAACGC 4915 0.0 25.899826 4 TATCAAC 4885 0.0 25.867098 2 ATCAACG 4915 0.0 25.803185 3 CAACGCA 4970 0.0 25.613207 5 AACGCAG 4985 0.0 25.345566 6 CTCCGAC 285 0.0 24.999537 94-95 GTGGTAT 1375 0.0 24.53191 1 CCTATAC 235 4.647518E-9 22.23363 3 ACGCAGA 5765 0.0 21.83394 7 CGCAGAG 5810 0.0 21.501322 8 TGGTATC 1425 0.0 21.335154 2 GTACATG 3535 0.0 21.234566 1 TACATGG 3640 0.0 20.228373 2 GAGTACT 4210 0.0 20.02635 12-13 CATGGGT 1425 0.0 19.999628 4 ACATGGG 3560 0.0 19.746824 3 >>END_MODULE