FastQCFastQC Report
Thu 26 May 2016
SRR938507_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938507_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1785245
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59370.33255939660942896No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47840.26797442367854274No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32540.1822719010555974No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28000.053.1518551
GTATCAA55050.044.0499081
ATCAACG67350.035.4413643
TCAACGC68550.034.8209464
TATCAAC68900.034.7820852
CAACGCA70150.034.026745
AACGCAG71800.033.4862026
ACGCAGA84200.028.3878337
CGCAGAG84550.027.9885188
TGGTATC13750.027.3192442
GTACATG60700.027.2598021
TACATGG59500.027.0112112
GTGGTAT15150.026.3632411
ACATGGG62850.024.5122993
GCAGAGT93300.024.4468989
GAGTACT56950.022.02796412-13
ACCGTAT1100.002871430721.5993928
TACCGTG1353.7863065E-421.1199957
GTACTTT59900.020.89905514-15
CAGAGTA88850.020.72481310-11
Produced by FastQC (version 0.11.5)