FastQCFastQC Report
Thu 26 May 2016
SRR938507_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938507_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1785245
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57930.32449327683315177No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50960.28545101652714333No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35320.1978439934014659No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29900.057.0647431
GTATCAA56400.042.5556141
ATCAACG71700.032.926883
TATCAAC72450.032.7345772
TCAACGC72550.032.5411074
CAACGCA74300.031.774665
TGGTATC12500.031.5582332
AACGCAG76300.031.2408036
ACGCAGA88300.026.8876157
CGCAGAG90150.026.2305018
GTGGTAT15950.026.2220481
GTACATG63700.023.7264231
GCAGAGT100150.023.184679
TACATGG64500.023.0637132
ACATGGG64550.022.3712733
TAAGGTG7150.021.9240725
GAGTACT59100.021.05022812-13
GTAAGGT7050.020.8874744
GTACTTT60750.020.67389914-15
GTCCTAC4650.020.441921
Produced by FastQC (version 0.11.5)