##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938507_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1785245 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.786263510050443 31.0 31.0 34.0 30.0 34.0 2 32.01104778335747 31.0 31.0 34.0 30.0 34.0 3 32.07071746454968 33.0 31.0 34.0 30.0 34.0 4 35.750870048648785 37.0 35.0 37.0 35.0 37.0 5 35.64280084806287 37.0 35.0 37.0 33.0 37.0 6 35.644912603032076 37.0 35.0 37.0 33.0 37.0 7 35.582243893695264 37.0 35.0 37.0 33.0 37.0 8 35.556576828390504 37.0 35.0 37.0 33.0 37.0 9 37.139057104206984 39.0 37.0 39.0 33.0 39.0 10-11 37.03586930645373 39.0 37.0 39.0 33.0 39.0 12-13 36.92081002887559 39.0 37.0 39.0 33.0 39.0 14-15 37.96039339138325 40.0 37.0 41.0 33.0 41.0 16-17 37.867092471901614 40.0 37.0 41.0 33.0 41.0 18-19 38.10489792717526 40.0 37.0 41.0 33.0 41.0 20-21 38.06713700360454 40.0 37.0 41.0 33.0 41.0 22-23 37.95755820629662 40.0 37.0 41.0 33.0 41.0 24-25 37.836324426059164 40.0 37.0 41.0 32.0 41.0 26-27 37.636321625323134 40.0 37.0 41.0 32.0 41.0 28-29 37.514075098936004 39.5 36.5 41.0 32.0 41.0 30-31 37.26004974107195 39.0 36.0 41.0 31.0 41.0 32-33 37.058718551235266 39.0 36.0 41.0 31.0 41.0 34-35 36.963800206694316 39.0 36.0 41.0 30.5 41.0 36-37 36.91992387599461 39.0 36.0 41.0 30.5 41.0 38-39 36.76270483883164 39.0 35.0 41.0 30.0 41.0 40-41 36.585034547078976 39.0 35.0 40.5 30.0 41.0 42-43 36.37201140459713 39.0 35.0 40.0 30.0 41.0 44-45 36.073810037277795 38.5 35.0 40.0 29.5 41.0 46-47 35.69003050001541 38.0 35.0 40.0 28.5 41.0 48-49 35.32648684074175 38.0 34.0 40.0 27.0 41.0 50-51 34.93693526658806 38.0 34.0 40.0 26.0 41.0 52-53 34.43698792042549 38.0 33.0 40.0 24.0 41.0 54-55 34.08659259653437 37.0 33.0 40.0 24.0 41.0 56-57 33.744251069180976 37.0 33.0 40.0 23.0 41.0 58-59 33.24523720833835 36.5 32.0 39.0 22.0 41.0 60-61 32.83471288254553 36.0 31.0 39.0 21.5 40.0 62-63 32.344336211556396 35.5 31.0 39.0 19.5 40.0 64-65 31.786898716982822 35.0 30.0 38.0 18.0 40.0 66-67 31.271305339043103 35.0 29.5 38.0 15.5 40.0 68-69 30.761178437693424 34.0 29.0 37.5 12.0 39.5 70-71 30.16706362432047 34.0 28.5 37.0 8.0 39.0 72-73 29.64593375138986 34.0 28.0 36.0 7.0 39.0 74-75 29.04650538161429 33.5 27.5 36.0 4.5 38.5 76-77 27.850486627885807 31.5 26.0 34.5 2.0 36.5 78-79 28.536619903710694 33.0 27.0 35.0 2.0 37.0 80-81 28.143696803519965 33.0 26.5 35.0 2.0 36.5 82-83 27.679808933787797 33.0 26.0 35.0 2.0 36.0 84-85 27.102982223728397 32.0 25.5 35.0 2.0 36.0 86-87 26.463821772361776 32.0 24.5 34.5 2.0 35.0 88-89 25.7057389882061 31.5 22.5 34.0 2.0 35.0 90-91 25.069840274024013 31.0 19.5 34.0 2.0 35.0 92-93 24.204605810406974 31.0 16.5 34.0 2.0 35.0 94-95 23.35940193082742 30.5 4.5 34.0 2.0 35.0 96-97 22.264928343168584 30.0 2.0 34.0 2.0 35.0 98-99 20.87883567801618 29.0 2.0 34.0 2.0 35.0 100-101 17.01532506742772 22.0 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1312370961783884 1101 2 0.2163476099196373 1101 3 0.2326989228448042 1101 4 0.1749493385273695 1101 5 -0.02929055204080555 1101 6 0.06655001617740197 1101 7 0.11614725442361618 1101 8 0.09078280828232721 1101 9 0.10347164503464512 1101 10-11 0.1039680867178916 1101 12-13 0.21763752323123953 1101 14-15 0.2880956780390491 1101 16-17 0.2657234811264928 1101 18-19 0.27492707548785233 1101 20-21 0.24978585905218154 1101 22-23 0.2241718229799261 1101 24-25 0.2741016591803245 1101 26-27 0.17508161128466782 1101 28-29 0.20636844090491024 1101 30-31 0.4124064953555475 1101 32-33 0.2336344569191411 1101 34-35 0.1501147475680611 1101 36-37 0.23409954618902162 1101 38-39 0.3689390847369154 1101 40-41 0.17566303017985518 1101 42-43 0.29596098488163847 1101 44-45 0.41382245027561027 1101 46-47 0.18067775786212792 1101 48-49 0.2031529336039526 1101 50-51 0.2868040236945788 1101 52-53 0.16701682731095246 1101 54-55 0.2878802276510015 1101 56-57 0.26377227552622173 1101 58-59 0.3544881788832015 1101 60-61 0.28552346638291226 1101 62-63 0.3225243499865158 1101 64-65 0.1338599368767106 1101 66-67 -0.06313396919598446 1101 68-69 0.2866360030119708 1101 70-71 0.3001384079805334 1101 72-73 0.23124810928570838 1101 74-75 0.34227977898371975 1101 76-77 0.29114804618518164 1101 78-79 0.41412523467114326 1101 80-81 0.39948364508187595 1101 82-83 0.22450038042349618 1101 84-85 0.691519223951218 1101 86-87 0.7621459400870307 1101 88-89 0.4798075183152939 1101 90-91 0.45554199981243215 1101 92-93 0.6726540403384433 1101 94-95 0.38434052058380175 1101 96-97 0.6484590810563624 1101 98-99 0.8135088012517855 1101 100-101 0.7923914305669513 1102 1 0.3159610887266773 1102 2 0.3827165968102406 1102 3 0.31384029070545694 1102 4 0.21994381334339153 1102 5 0.10995567771674786 1102 6 0.15100318196206786 1102 7 0.12415049003494971 1102 8 0.2604586244063256 1102 9 0.23877439066683337 1102 10-11 0.3852211507437815 1102 12-13 0.3510960565267638 1102 14-15 0.5202847440047051 1102 16-17 0.4351455627826155 1102 18-19 0.3504793288884258 1102 20-21 0.33118493633320867 1102 22-23 0.43088227154005665 1102 24-25 0.3860636493595493 1102 26-27 0.39997432069178274 1102 28-29 0.31380792887802045 1102 30-31 0.40664397359268634 1102 32-33 0.40277779503124833 1102 34-35 0.3989414740886801 1102 36-37 0.6260340031972547 1102 38-39 0.6295114646469742 1102 40-41 0.5573548627124509 1102 42-43 0.30260412424612326 1102 44-45 0.4633347898531497 1102 46-47 0.43224239853967106 1102 48-49 0.3259002948979841 1102 50-51 0.4267717073307651 1102 52-53 0.34227629233534174 1102 54-55 0.32005399004953716 1102 56-57 0.4420426014458201 1102 58-59 0.5107692331996105 1102 60-61 0.4489562883606908 1102 62-63 0.7124635464631197 1102 64-65 0.6021393190630953 1102 66-67 0.5072882767581746 1102 68-69 0.7032323903101414 1102 70-71 0.680663591954584 1102 72-73 0.8856463611015997 1102 74-75 0.8689866567115274 1102 76-77 0.7196538880403693 1102 78-79 0.9806808455815919 1102 80-81 0.932801365685112 1102 82-83 1.1258021442424173 1102 84-85 1.3195489658708013 1102 86-87 1.143318378826386 1102 88-89 1.104026605772198 1102 90-91 1.0797577748089218 1102 92-93 1.775554132557339 1102 94-95 2.0904158048591555 1102 96-97 1.6538461354310634 1102 98-99 1.9300060466097015 1102 100-101 1.9905724591786615 1103 1 0.31962864341147323 1103 2 0.270101132121475 1103 3 0.31050497598639026 1103 4 0.22979542251166407 1103 5 0.1280580741387567 1103 6 0.16254149820608887 1103 7 0.16149503708597734 1103 8 0.18623124376490807 1103 9 0.23057558653698607 1103 10-11 0.36336515303749906 1103 12-13 0.38482777646752453 1103 14-15 0.6028917396416773 1103 16-17 0.38159939353525374 1103 18-19 0.4101210844456986 1103 20-21 0.4018324232720474 1103 22-23 0.3451948722170073 1103 24-25 0.36002446312467384 1103 26-27 0.38020588129296584 1103 28-29 0.46692938631692016 1103 30-31 0.3479396900864131 1103 32-33 0.36564118047578376 1103 34-35 0.3666476848450557 1103 36-37 0.4259076938925048 1103 38-39 0.3333020998710623 1103 40-41 0.36456333990074086 1103 42-43 0.3264417767528158 1103 44-45 0.2254616293211953 1103 46-47 0.22366689434372233 1103 48-49 0.2917912937133522 1103 50-51 0.48784671187524964 1103 52-53 0.41892201830550135 1103 54-55 0.1844285668794612 1103 56-57 0.33462092887428696 1103 58-59 0.24589261562847042 1103 60-61 0.3734769539125722 1103 62-63 0.5061649676688091 1103 64-65 0.4876317978153928 1103 66-67 0.7406079093634084 1103 68-69 0.4129227872190526 1103 70-71 0.30884106040227977 1103 72-73 0.4934482208059272 1103 74-75 0.6581971880404005 1103 76-77 0.40489026111647064 1103 78-79 0.6247389969390156 1103 80-81 0.6255323491444678 1103 82-83 0.8701806214210173 1103 84-85 0.8538751015325481 1103 86-87 1.0574199796805885 1103 88-89 0.9131342428034266 1103 90-91 0.9480565931776255 1103 92-93 1.0093462949874876 1103 94-95 0.9780933506357847 1103 96-97 1.1175895209014257 1103 98-99 1.251089174728083 1103 100-101 1.5784959400202965 1104 1 0.1874159478411137 1104 2 0.29796674057102734 1104 3 0.29214966390844666 1104 4 0.16647630328014174 1104 5 0.1299901803044392 1104 6 0.10321709800577139 1104 7 0.2722931007307565 1104 8 0.18295431158286135 1104 9 0.24783707036781522 1104 10-11 0.25708429047926273 1104 12-13 0.3388303866015221 1104 14-15 0.33378100622437756 1104 16-17 0.4812640216670374 1104 18-19 0.41878870209141184 1104 20-21 0.3946581968277485 1104 22-23 0.343004180683252 1104 24-25 0.44635692135494054 1104 26-27 0.40230324536113926 1104 28-29 0.3505934377911899 1104 30-31 0.30765029906709174 1104 32-33 0.3578669267161345 1104 34-35 0.5209081215872473 1104 36-37 0.5731875397124924 1104 38-39 0.4349628735026414 1104 40-41 0.718884477433555 1104 42-43 0.8440647337918321 1104 44-45 0.7845653821475693 1104 46-47 0.6527829390802111 1104 48-49 0.7971481384862571 1104 50-51 0.7680713048005714 1104 52-53 0.7545822152795623 1104 54-55 0.9073972907970926 1104 56-57 0.8172639930501902 1104 58-59 0.8244150066326199 1104 60-61 0.8441546782399314 1104 62-63 0.8268281238064148 1104 64-65 1.032691360466199 1104 66-67 0.9107270346650189 1104 68-69 0.9925940924377983 1104 70-71 0.962497979533655 1104 72-73 0.7939212317168973 1104 74-75 0.8589521541238341 1104 76-77 0.7144037442795899 1104 78-79 0.910410575311321 1104 80-81 0.9974304628674027 1104 82-83 1.2795399245759391 1104 84-85 1.4114983620755162 1104 86-87 1.3816795059109168 1104 88-89 1.428264673627286 1104 90-91 1.4173943475518769 1104 92-93 1.5344615506136918 1104 94-95 1.6642685938183277 1104 96-97 1.5514252038611964 1104 98-99 1.6842498648960706 1104 100-101 1.6254718266312196 1105 1 0.09042917383306204 1105 2 0.10419445764980395 1105 3 0.13165225160252447 1105 4 0.12642742461423495 1105 5 0.025050559832905606 1105 6 0.024249300420954967 1105 7 0.11298027726899562 1105 8 0.07438904418781078 1105 9 0.18272206174216166 1105 10-11 0.18495519329697174 1105 12-13 0.304632571765417 1105 14-15 0.3683008452122962 1105 16-17 0.23513837474351362 1105 18-19 0.26747904136686174 1105 20-21 0.236777230755294 1105 22-23 0.24732850500757309 1105 24-25 0.39175657632906535 1105 26-27 0.15340301534388345 1105 28-29 0.18944766435818394 1105 30-31 0.3881994652545586 1105 32-33 0.4377476052670204 1105 34-35 0.4557341710409517 1105 36-37 0.39529794799311446 1105 38-39 0.2995229655095386 1105 40-41 0.36958890526564403 1105 42-43 0.5914482875583644 1105 44-45 0.4581809658163465 1105 46-47 0.40945631458337317 1105 48-49 0.6353166261228225 1105 50-51 0.6234436452261036 1105 52-53 0.6666221807769759 1105 54-55 0.6177250653801849 1105 56-57 0.5648790016758412 1105 58-59 0.6931010330846021 1105 60-61 0.7020471451749586 1105 62-63 0.7480587161008359 1105 64-65 0.5839751442499796 1105 66-67 0.31015630436024466 1105 68-69 0.6869730458593075 1105 70-71 0.7760416828113996 1105 72-73 0.6913177239538122 1105 74-75 0.7597140862687475 1105 76-77 0.6261159351939085 1105 78-79 0.8673326569674451 1105 80-81 0.771077673908227 1105 82-83 0.8707561407921567 1105 84-85 1.0390716800743682 1105 86-87 1.1034894541570388 1105 88-89 1.1954009588486798 1105 90-91 1.3792330479544077 1105 92-93 1.4587053689000626 1105 94-95 1.6162639934733 1105 96-97 1.7458889761441156 1105 98-99 1.77747585684547 1105 100-101 1.9022673987991325 1106 1 0.0028895135120450277 1106 2 0.14832473013954228 1106 3 0.10755744010570112 1106 4 -0.014635005396961276 1106 5 -0.0036821762626502164 1106 6 0.011914940585192824 1106 7 0.032220198881539375 1106 8 0.07887299342259979 1106 9 -0.23358066315518755 1106 10-11 0.07175297231899691 1106 12-13 -0.028081360672068456 1106 14-15 0.1506426705202486 1106 16-17 0.13990111270543792 1106 18-19 0.08101487563337884 1106 20-21 -0.008864799853142813 1106 22-23 0.2019337681654605 1106 24-25 0.1884414400841976 1106 26-27 0.08394146252567225 1106 28-29 0.14938374125254938 1106 30-31 0.04081471348150956 1106 32-33 -0.02525748058591404 1106 34-35 0.12543485226529327 1106 36-37 0.15296172178931045 1106 38-39 0.17009267435612685 1106 40-41 0.18161414789349095 1106 42-43 0.3290591031612067 1106 44-45 0.31677792363642965 1106 46-47 -0.4101592135987886 1106 48-49 -0.06452356611308119 1106 50-51 0.32134981357250325 1106 52-53 0.3923104039945926 1106 54-55 0.6235189716885117 1106 56-57 0.44516198973108345 1106 58-59 0.15648055902711633 1106 60-61 0.39297076406259634 1106 62-63 0.227344363730559 1106 64-65 0.26018128460085777 1106 66-67 0.25034504082907816 1106 68-69 0.14319271737853256 1106 70-71 0.2833310165180052 1106 72-73 0.6707351838269027 1106 74-75 0.6164038492400046 1106 76-77 0.33959577841899247 1106 78-79 0.6268697976916009 1106 80-81 -0.009932031205618586 1106 82-83 0.5724557021956649 1106 84-85 0.7449087461437962 1106 86-87 0.8250261749483769 1106 88-89 1.00959468785204 1106 90-91 1.1621371166367496 1106 92-93 1.033949163226307 1106 94-95 1.1646974107931989 1106 96-97 1.4550913370996987 1106 98-99 1.5579909744266551 1106 100-101 1.3758232965221637 1107 1 0.20173702270583505 1107 2 0.24228823927830234 1107 3 0.17320361906590875 1107 4 0.11403878473702633 1107 5 0.03438034150589431 1107 6 0.10051722092011772 1107 7 0.11412946924772882 1107 8 0.22455615388517458 1107 9 0.13650719370599518 1107 10-11 0.21993547935763758 1107 12-13 0.21135366262192434 1107 14-15 0.38157730749166063 1107 16-17 0.3597511863016152 1107 18-19 0.2251292454575733 1107 20-21 0.3611955444210295 1107 22-23 0.3199369733818571 1107 24-25 0.34122772001560264 1107 26-27 0.31372085743217326 1107 28-29 0.3950244291589371 1107 30-31 0.29492583911314796 1107 32-33 0.3817137217600006 1107 34-35 0.5789547725246464 1107 36-37 0.5040249113235191 1107 38-39 0.44133153175837947 1107 40-41 0.5379022929490205 1107 42-43 0.4747862551040427 1107 44-45 0.4945937795988655 1107 46-47 0.4118767636941598 1107 48-49 0.4846490214915775 1107 50-51 0.5810143262328751 1107 52-53 0.5971247054958653 1107 54-55 0.3966414382377934 1107 56-57 0.5824935037946091 1107 58-59 0.40968548882416655 1107 60-61 0.48880154674984055 1107 62-63 0.8534451538003438 1107 64-65 0.5308452435704218 1107 66-67 0.5512898975802081 1107 68-69 0.9791116607117729 1107 70-71 1.065828110501002 1107 72-73 1.049813208377298 1107 74-75 0.8460185985086248 1107 76-77 0.7697825888847802 1107 78-79 0.9458409874275695 1107 80-81 1.0894634943306478 1107 82-83 1.0399537361920537 1107 84-85 1.1334844882958315 1107 86-87 1.143313418966681 1107 88-89 1.3517377808170714 1107 90-91 1.5426631343731998 1107 92-93 1.7963614955141622 1107 94-95 1.9204253307957657 1107 96-97 2.1080603178042026 1107 98-99 1.8348019904664952 1107 100-101 1.4053405767195244 1108 1 0.12597333258742083 1108 2 0.19073429640047124 1108 3 0.18838348730839982 1108 4 0.1649984736589829 1108 5 0.09373053303502132 1108 6 0.12275520288589803 1108 7 0.10389036574821375 1108 8 0.06400428663935287 1108 9 0.19737253417037692 1108 10-11 0.11147845663238343 1108 12-13 0.14669023341902943 1108 14-15 0.336655085515396 1108 16-17 0.4004954680971764 1108 18-19 0.2551748276306327 1108 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-1.0292261202077242 2316 80-81 -1.0986188217112947 2316 82-83 -1.1972721816479286 2316 84-85 -1.3569789387547253 2316 86-87 -1.2565746305785126 2316 88-89 -1.2185041540238934 2316 90-91 -1.2002998172011132 2316 92-93 -1.713361566621188 2316 94-95 -1.3115578208316165 2316 96-97 -1.6550433234896857 2316 98-99 -1.6144376461443812 2316 100-101 -1.7251864787329652 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 11.0 4 19.0 5 65.0 6 125.0 7 363.0 8 836.0 9 1489.0 10 2499.0 11 3717.0 12 5083.0 13 7192.0 14 9672.0 15 12364.0 16 15647.0 17 19291.0 18 19007.0 19 16999.0 20 17139.0 21 18241.0 22 19927.0 23 22147.0 24 25385.0 25 30187.0 26 35418.0 27 42214.0 28 50565.0 29 59200.0 30 69514.0 31 82076.0 32 98431.0 33 120108.0 34 150966.0 35 193661.0 36 246750.0 37 264307.0 38 120044.0 39 4579.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.64131729214816 19.11549048362179 14.831075744522579 23.412116479707468 2 16.33433537103416 20.312615587402572 38.81892932159582 24.53411971996745 3 19.509759164708484 23.703637315886613 29.67284602393509 27.11375749546981 4 13.62882965643371 16.756971732171213 35.44247428224137 34.171724329153705 5 14.132404110890251 36.950153858568925 33.95945262103158 14.957989409509251 6 33.4132289965803 35.58984901232044 16.613630061980288 14.383291929118972 7 29.625345540808123 32.02865713109405 19.9019182241093 18.44407910398853 8 26.898044806175065 35.55680032712597 18.90698475559377 18.638170111105197 9 26.391839775492997 16.563440872261232 19.38036515996404 37.66435419228173 10-11 25.57237241947184 26.359743340549898 26.80466826682052 21.263215973157745 12-13 26.282891143792586 23.330075143747777 27.447913311618294 22.939120400841343 14-15 22.719094578055113 25.084484202448404 25.41469098078975 26.78173023870673 16-17 22.65100868508244 28.321882990849996 26.287120255203067 22.7399880688645 18-19 22.728953168892787 26.544703948197583 28.564538760786334 22.161804122123293 20-21 23.08523479970536 25.82861175917031 28.810947517007467 22.275205924116857 22-23 22.968080011427002 25.656814610879742 28.803497559158547 22.571607818534712 24-25 22.866084135161426 25.899124410165143 28.807076421758637 22.427715032914794 26-27 22.44921051393336 26.351072029540468 28.57044429457118 22.629273161954995 28-29 22.52967172265296 26.538088501652574 28.57542962626667 22.356810149427794 30-31 22.597622367202717 26.1456704800134 28.477678673956895 22.77902847882699 32-33 22.323158032710484 26.16527880316238 28.532106083334547 22.97945708079259 34-35 22.987309024743315 26.306687551259127 28.269631965078812 22.436371458918753 36-37 22.997349352633588 25.884823741194623 28.357948888616225 22.759878017555565 38-39 22.58042561105371 26.24721000664081 28.37532129212018 22.7970430901853 40-41 22.89529566828196 26.000587040191526 28.482008170389534 22.622109121136976 42-43 22.76921551581202 26.055525805165065 28.261075360851073 22.914183318171837 44-45 22.694288936984396 26.316819930250624 28.22351396037937 22.76537717238561 46-47 22.948026741427647 26.60013051429916 27.70185604776935 22.74998669650384 48-49 22.927413324221604 26.60391150794429 27.643600738274017 22.82507442956009 50-51 22.99979274495306 26.67302435526876 27.586879075082344 22.74030382469584 52-53 23.28882030197536 26.54590826469196 27.41464056754115 22.75063086579153 54-55 22.89233495890147 26.670044397839597 27.500081222391387 22.937539420867544 56-57 22.791437589798598 26.938851530182134 27.406126329999523 22.863584550019745 58-59 22.908616310868254 26.515595909816962 27.423762885471625 23.152024893843155 60-61 22.625409957736895 26.60626412621237 27.775683449610554 22.99264246644018 62-63 22.694522740010882 26.693670117398067 27.77675852713645 22.835048615454596 64-65 22.494701642084788 26.630305991024024 27.63661584089968 23.238376525991505 66-67 22.43259541507937 26.805991369277777 27.536692613357705 23.22472060228515 68-69 22.68062924606497 26.501439947024068 27.727965000925792 23.089965805985173 70-71 22.665345838073584 26.520955410551956 27.399015148077883 23.41468360329658 72-73 22.630877731313554 26.720037507467463 27.26606915747395 23.383015603745033 74-75 22.566871704597467 26.99948399690062 27.103637065494297 23.330007233007617 76-77 22.623916949983204 26.713454408711645 27.33952954277153 23.32309909853363 78-79 22.629640431111742 26.90646704033569 27.350342585970072 23.113549942582495 80-81 22.69982510689546 26.903646870007332 27.196956305298425 23.199571717798783 82-83 22.660626978165617 26.969760559485543 27.16145193469045 23.208160527658386 84-85 22.664060845567843 26.864784888105063 27.127505212949405 23.34364905337769 86-87 22.768307555618296 26.845152764402947 27.063099521643142 23.323440158335615 88-89 22.59874732485603 26.950847426746254 26.87177388498878 23.578631363408935 90-91 22.54498371142968 26.884811275853497 27.089423396656453 23.48078161606037 92-93 22.570864776341672 26.634191354645758 27.119528160179918 23.675415708832652 94-95 22.406548915723135 26.540522550627976 27.222817999264898 23.83011053438399 96-97 22.578607101080785 26.359116602576226 27.20665139703153 23.855624899311465 98-99 22.34518023099741 26.5577810170544 26.884202585929096 24.21283616601909 100-101 22.428994998592437 26.41947677797868 27.25670484132331 23.894823382105574 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 487.0 1 343.5 2 291.0 3 416.0 4 369.5 5 339.0 6 619.0 7 1349.5 8 2029.0 9 2246.5 10 1956.5 11 1514.5 12 1418.5 13 1544.5 14 1733.5 15 1837.0 16 1931.5 17 2081.5 18 2268.5 19 2476.5 20 2734.0 21 3196.0 22 3821.0 23 4602.5 24 5668.0 25 7008.0 26 8763.5 27 11097.5 28 13348.0 29 15668.5 30 18309.0 31 21396.5 32 25736.5 33 28266.5 34 29066.5 35 31232.5 36 34533.5 37 38081.5 38 41503.0 39 43821.5 40 46393.0 41 51253.0 42 55258.0 43 58045.0 44 61267.5 45 65018.5 46 66531.5 47 67656.5 48 69880.0 49 70107.0 50 69098.0 51 67120.0 52 65795.0 53 65903.0 54 66246.0 55 62820.5 56 56977.5 57 52030.0 58 47947.0 59 42694.5 60 35257.5 61 29038.0 62 24056.5 63 18441.5 64 13647.5 65 10537.5 66 7750.0 67 6012.5 68 4626.0 69 3381.5 70 2623.5 71 1896.5 72 1428.0 73 1119.5 74 835.5 75 630.0 76 425.0 77 263.5 78 160.0 79 97.5 80 57.5 81 27.0 82 12.0 83 8.0 84 5.0 85 1.5 86 1.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.9210304468014195E-4 2 0.04934896890902929 3 0.0 4 0.0 5 0.045595982624233644 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005909553030536425 26-27 0.0018484857820635263 28-29 8.402208100288756E-5 30-31 0.001344353296046201 32-33 3.9210304468014195E-4 34-35 0.011034899971712565 36-37 0.022293858826099498 38-39 0.003949037807135716 40-41 0.0010082649720346507 42-43 0.0011763091340404258 44-45 0.007814053533268544 46-47 0.0 48-49 0.0 50-51 2.8007360334295853E-4 52-53 0.0 54-55 0.0012883385753776093 56-57 0.0 58-59 0.0216216821780764 60-61 0.0 62-63 0.009214421549983335 64-65 0.014451797932496659 66-67 0.006189626633879384 68-69 0.01588017330954575 70-71 1.1202944133718341E-4 72-73 2.8007360334295853E-5 74-75 0.020837476088716115 76-77 0.048984873224683444 78-79 0.028595514901316066 80-81 0.024674484454514645 82-83 0.033916913364832275 84-85 0.014955930418513987 86-87 0.00985859083767214 88-89 0.08192152897781538 90-91 0.022657954510445343 92-93 0.022517917708773864 94-95 0.025822786228220776 96-97 0.03682967883959905 98-99 0.05018918971905817 100-101 0.013863643365476448 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1785245.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.676921713343795 #Duplication Level Percentage of deduplicated Percentage of total 1 78.7674395702859 44.642960060856325 2 12.01570621764801 13.620264812563493 3 3.8163701604447278 6.489003384380014 4 1.6837372184206736 3.817161700570871 5 0.9271130059721315 2.6272955629452666 6 0.563030281649039 1.9146493917158698 7 0.40244005043098274 1.5966344262813656 8 0.2724078057806454 1.2351388705866722 9 0.213357387085509 1.0883195932328087 >10 1.2035664318738513 12.571696146429609 >50 0.08818458632500435 3.3892731654784707 >100 0.04229016979547314 4.316039961591132 >500 0.0031688103913402365 1.2282286843419459 >1k 9.902532472938238E-4 0.8521944956611271 >5k 1.9805064945876478E-4 0.6111397433649903 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5793 0.32449327683315177 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5096 0.28545101652714333 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3532 0.1978439934014659 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 5.601472066859171E-5 0.0 0.0 0.0 0.0 9 5.601472066859171E-5 0.0 0.0 0.0 0.0 10-11 5.601472066859171E-5 0.0 0.0 0.0 0.0 12-13 5.601472066859171E-5 0.0 0.0 0.0 8.402208100288756E-5 14-15 5.601472066859171E-5 0.0 0.0 0.0 1.1202944133718341E-4 16-17 5.601472066859171E-5 0.0 0.0 0.0 1.1202944133718341E-4 18-19 8.402208100288756E-5 0.0 0.0 0.0 1.1202944133718341E-4 20-21 1.4003680167147927E-4 0.0 0.0 0.0 2.2405888267436683E-4 22-23 2.2405888267436683E-4 0.0 0.0 5.601472066859171E-5 2.520662430086627E-4 24-25 3.3608832401155024E-4 0.0 0.0 2.8007360334295853E-4 3.080809636772544E-4 26-27 3.9210304468014195E-4 0.0 0.0 9.242428910317632E-4 3.3608832401155024E-4 28-29 4.761251256830295E-4 0.0 0.0 0.002828743393763881 3.3608832401155024E-4 30-31 5.041324860173254E-4 0.0 0.0 0.009438480432657702 3.3608832401155024E-4 32-33 5.601472066859171E-4 0.0 0.0 0.023078064915459783 3.3608832401155024E-4 34-35 7.001840083573963E-4 0.0 0.0 0.043579452680164346 3.9210304468014195E-4 36-37 7.842060893602839E-4 0.0 0.0 0.079708947511406 3.9210304468014195E-4 38-39 8.402208100288756E-4 0.0 0.0 0.13457536640629156 5.041324860173254E-4 40-41 8.402208100288756E-4 0.0 0.0 0.19423104391834173 5.041324860173254E-4 42-43 8.962355306974673E-4 0.0 0.0 0.2564353912208128 5.321398463516212E-4 44-45 0.0010082649720346507 0.0 0.0 0.3359202798495444 5.601472066859171E-4 46-47 0.0012323238547090175 0.0 0.0 0.43467423238827163 5.601472066859171E-4 48-49 0.0012323238547090175 0.0 0.0 0.5414943047032761 6.161619273545088E-4 50-51 0.0012323238547090175 0.0 0.0 0.6549241140571742 6.721766480231005E-4 52-53 0.0012603312150433134 0.0 0.0 0.7622203114978616 7.842060893602839E-4 54-55 0.0012883385753776093 0.0 0.0 0.879543143938227 7.842060893602839E-4 56-57 0.001344353296046201 0.0 0.0 1.0219325638777872 8.122134496945797E-4 58-59 0.001344353296046201 0.0 0.0 1.1817705693056135 8.402208100288756E-4 60-61 0.001344353296046201 0.0 0.0 1.3571526597189743 8.402208100288756E-4 62-63 0.001344353296046201 0.0 0.0 1.532842831096012 8.962355306974673E-4 64-65 0.001344353296046201 0.0 0.0 1.7131542169282086 9.242428910317632E-4 66-67 0.001512397458051976 0.0 0.0 1.9217530366980442 9.52250251366059E-4 68-69 0.001512397458051976 0.0 0.0 2.156034605894429 9.52250251366059E-4 70-71 0.001512397458051976 0.0 0.0 2.402639413637904 0.0010082649720346507 72-73 0.001540404818386272 0.0 0.0 2.6554058406549244 0.00114830177370613 74-75 0.0015684121787205678 0.0 0.0 2.9146699752695007 0.0011763091340404258 76-77 0.0015684121787205678 0.0 0.0 3.1980764544922407 0.0011763091340404258 78-79 0.0015964195390548636 2.8007360334295853E-5 0.0 3.510470551660976 0.0012323238547090175 80-81 0.001736456340726343 5.601472066859171E-5 0.0 3.8539808261611155 0.0012323238547090175 82-83 0.001736456340726343 5.601472066859171E-5 0.0 4.204941058510176 0.0012323238547090175 84-85 0.001736456340726343 5.601472066859171E-5 0.0 4.5620629101327825 0.0012323238547090175 86-87 0.001736456340726343 5.601472066859171E-5 0.0 4.932404235833177 0.0012603312150433134 88-89 0.001736456340726343 5.601472066859171E-5 0.0 5.338314909158127 0.001344353296046201 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2990 0.0 57.064743 1 GTATCAA 5640 0.0 42.555614 1 ATCAACG 7170 0.0 32.92688 3 TATCAAC 7245 0.0 32.734577 2 TCAACGC 7255 0.0 32.541107 4 CAACGCA 7430 0.0 31.77466 5 TGGTATC 1250 0.0 31.558233 2 AACGCAG 7630 0.0 31.240803 6 ACGCAGA 8830 0.0 26.887615 7 CGCAGAG 9015 0.0 26.230501 8 GTGGTAT 1595 0.0 26.222048 1 GTACATG 6370 0.0 23.726423 1 GCAGAGT 10015 0.0 23.18467 9 TACATGG 6450 0.0 23.063713 2 ACATGGG 6455 0.0 22.371273 3 TAAGGTG 715 0.0 21.924072 5 GAGTACT 5910 0.0 21.050228 12-13 GTAAGGT 705 0.0 20.887474 4 GTACTTT 6075 0.0 20.673899 14-15 GTCCTAC 465 0.0 20.44192 1 >>END_MODULE