FastQCFastQC Report
Thu 26 May 2016
SRR938506_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938506_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1779742
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59160.3324077310082023No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48180.27071339553710594No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32450.18232979836403254No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29850.056.28861
GTATCAA51700.041.797991
ATCAACG66500.031.9405733
TATCAAC66850.031.7022652
TCAACGC67700.031.3744184
CAACGCA69250.030.8085445
AACGCAG70550.030.1684066
ACGCAGA81600.026.0219527
CGCAGAG83050.025.5676278
TACATGG61050.024.2064722
GTACATG62300.024.066481
ACATGGG61550.023.0834083
GTGTAAG7000.022.4391271
GAGTACT53700.022.33433312-13
GCAGAGT93500.022.1499639
GTATAGG4350.021.8842071
TGGTATC14500.021.6288152
GTGGTAT15700.020.9189321
CAGAGTA88500.020.74398410-11
TAAGGTG8750.020.6357465
Produced by FastQC (version 0.11.5)