##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938506_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1779742 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.922945573010022 31.0 31.0 34.0 27.0 34.0 2 31.010545348707847 31.0 31.0 34.0 27.0 34.0 3 31.07511088685888 31.0 31.0 34.0 27.0 34.0 4 34.707304766645954 37.0 35.0 37.0 32.0 37.0 5 34.715634063813745 37.0 35.0 37.0 32.0 37.0 6 34.74412358645242 37.0 35.0 37.0 31.0 37.0 7 34.65624399491612 37.0 35.0 37.0 30.0 37.0 8 34.61947911551225 37.0 35.0 37.0 30.0 37.0 9 36.09917954400132 39.0 35.0 39.0 30.0 39.0 10-11 35.94167132089932 38.0 35.0 39.0 30.0 39.0 12-13 35.77456395365171 38.0 35.0 39.0 30.0 39.0 14-15 36.73243649922293 39.0 36.0 41.0 30.0 41.0 16-17 36.576193347125596 39.0 36.0 41.0 30.0 41.0 18-19 36.5563837342716 39.0 36.0 40.5 30.0 41.0 20-21 36.645901765536806 39.0 36.0 41.0 30.0 41.0 22-23 36.561036655874844 39.0 36.0 41.0 30.0 41.0 24-25 36.43147658480836 39.0 36.0 41.0 29.5 41.0 26-27 36.230716025131734 39.0 35.0 41.0 29.0 41.0 28-29 36.02726406411716 39.0 35.0 40.0 27.0 41.0 30-31 35.79649803173719 38.5 35.0 40.0 27.0 41.0 32-33 35.50812814441644 38.0 34.0 40.0 27.0 41.0 34-35 35.245520418128024 38.0 34.0 40.0 25.5 41.0 36-37 34.9455960470675 38.0 34.0 40.0 25.0 41.0 38-39 34.666895819731174 38.0 33.0 40.0 24.5 41.0 40-41 34.58464850523278 38.0 33.0 40.0 24.0 41.0 42-43 34.392126218294564 38.0 33.0 40.0 24.0 41.0 44-45 34.09294774186371 38.0 33.0 40.0 23.0 41.0 46-47 33.708052065973604 38.0 33.0 40.0 21.0 41.0 48-49 33.32425430202805 38.0 32.0 40.0 18.5 41.0 50-51 32.58291651261813 36.5 30.5 39.5 16.0 40.5 52-53 32.638899346085 37.0 31.0 39.0 15.5 40.0 54-55 32.74979856630905 37.0 31.0 40.0 14.5 41.0 56-57 32.42724844387557 37.0 31.0 40.0 11.0 41.0 58-59 32.06453407291619 36.0 31.0 39.0 8.5 41.0 60-61 31.601767278628024 36.0 30.0 39.0 7.5 41.0 62-63 31.05567408084992 35.0 29.0 39.0 4.0 40.0 64-65 30.537499536449666 35.0 29.0 38.5 2.0 40.0 66-67 29.98326133787931 34.5 28.0 38.0 2.0 40.0 68-69 29.403397233981106 34.0 27.5 37.5 2.0 40.0 70-71 28.766670955677846 34.0 26.0 37.0 2.0 39.0 72-73 28.19790452773492 34.0 26.0 36.0 2.0 39.0 74-75 27.56765755935411 33.0 25.5 36.0 2.0 38.5 76-77 26.822128432098587 32.5 23.5 35.0 2.0 37.5 78-79 26.020895163456274 32.0 21.0 35.0 2.0 37.0 80-81 25.351820376211833 31.0 19.0 35.0 2.0 36.0 82-83 24.684586586145635 31.0 16.5 34.5 2.0 36.0 84-85 24.014457432594163 31.0 9.0 34.0 2.0 35.0 86-87 23.328635274101526 30.0 4.5 34.0 2.0 35.0 88-89 22.523246066002827 29.5 2.0 34.0 2.0 35.0 90-91 21.68172914950594 29.0 2.0 34.0 2.0 35.0 92-93 20.828908628329273 29.0 2.0 34.0 2.0 35.0 94-95 19.864260100621326 26.5 2.0 33.5 2.0 35.0 96-97 18.628694215228947 25.0 2.0 33.0 2.0 34.5 98-99 17.16541302053893 20.5 2.0 32.0 2.0 34.0 100-101 13.890639205008366 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.67707452227544 1101 2 0.6790909680773183 1101 3 0.6614729191403406 1101 4 0.6567603484410824 1101 5 0.7034680526454338 1101 6 0.802463680248465 1101 7 0.5374525634628498 1101 8 0.6004564261066818 1101 9 0.6608665004500907 1101 10-11 0.5109530315487874 1101 12-13 0.6435203953181485 1101 14-15 0.8924936253916655 1101 16-17 0.9989584014797259 1101 18-19 0.7990948237740341 1101 20-21 0.9086540354496364 1101 22-23 0.9955829612937706 1101 24-25 0.9484285215865498 1101 26-27 1.0791109561445822 1101 28-29 0.9247022044118793 1101 30-31 0.9736795957914737 1101 32-33 1.0138086000535225 1101 34-35 0.9493926371504315 1101 36-37 0.9529425093677091 1101 38-39 1.0285818957999027 1101 40-41 0.9331083179372186 1101 42-43 0.9297939013979075 1101 44-45 1.0810641370204692 1101 46-47 1.061515411625635 1101 48-49 1.103218721544664 1101 50-51 1.0644888416466003 1101 52-53 1.1861148584692316 1101 54-55 1.2260953817321862 1101 56-57 1.1761505563609518 1101 58-59 1.0002499345939455 1101 60-61 0.9341691822708427 1101 62-63 0.9151451252966822 1101 64-65 1.0353251434972748 1101 66-67 1.1104328355562174 1101 68-69 0.8961179107577024 1101 70-71 1.26732904817041 1101 72-73 1.3460393851878827 1101 74-75 0.9792953962225752 1101 76-77 1.2410539926692543 1101 78-79 1.1373486462608042 1101 80-81 1.2229763147941632 1101 82-83 1.0257581563967761 1101 84-85 1.175454215562283 1101 86-87 1.3856662196561977 1101 88-89 1.5969437928086307 1101 90-91 1.583842941146905 1101 92-93 1.2856535224576469 1101 94-95 1.2312253279798036 1101 96-97 1.114904081729474 1101 98-99 0.9845194398549921 1101 100-101 0.8603760243188479 1102 1 0.5432961277058155 1102 2 0.5136447209704507 1102 3 0.47727992026974775 1102 4 0.7009235088526253 1102 5 0.6251165566524008 1102 6 0.5383877151041645 1102 7 0.4464416381838987 1102 8 0.5249763956947859 1102 9 0.7165646277852318 1102 10-11 0.6416589282860983 1102 12-13 0.6553321935384488 1102 14-15 0.7920656573894078 1102 16-17 0.768559787481756 1102 18-19 0.8476057185685804 1102 20-21 0.5791283957633624 1102 22-23 0.6912433670691343 1102 24-25 0.6243333891705589 1102 26-27 0.6345251666200795 1102 28-29 0.6210611148698106 1102 30-31 0.6728654810864256 1102 32-33 0.6339639287758558 1102 34-35 0.9253757095757678 1102 36-37 0.8273528834228188 1102 38-39 0.8792103193214729 1102 40-41 0.9050715692417057 1102 42-43 0.9032353988706703 1102 44-45 0.9940118992843452 1102 46-47 0.9321798298069979 1102 48-49 0.7731905012304523 1102 50-51 0.7713337261502815 1102 52-53 1.0021314604375675 1102 54-55 0.9650022738098372 1102 56-57 0.8162281811747931 1102 58-59 1.025947129271266 1102 60-61 0.8946038930925369 1102 62-63 0.7446911977691393 1102 64-65 0.706079795498912 1102 66-67 0.8960672027116345 1102 68-69 0.7920107229978655 1102 70-71 0.9405440383214767 1102 72-73 0.9927647510748194 1102 74-75 0.989800649590773 1102 76-77 0.8773573193575395 1102 78-79 1.02576569524577 1102 80-81 1.155444143866653 1102 82-83 1.145949901617069 1102 84-85 1.118783059175339 1102 86-87 1.0502051851442324 1102 88-89 1.096673882294347 1102 90-91 1.2074530585398549 1102 92-93 1.354075178706335 1102 94-95 1.2408923427728134 1102 96-97 1.2162721226767808 1102 98-99 1.4353794129825808 1102 100-101 0.9048641319429471 1103 1 0.6237310227951376 1103 2 0.6676037015360485 1103 3 0.6915577609116959 1103 4 0.6871041392390396 1103 5 0.5546406961193782 1103 6 0.5436045665015072 1103 7 0.5952377580522494 1103 8 0.43743722431705834 1103 9 0.5784631657620665 1103 10-11 0.5800780355948731 1103 12-13 0.6170032956911058 1103 14-15 0.8618351395960957 1103 16-17 0.7231884776898667 1103 18-19 0.7792996092959044 1103 20-21 0.7990178947971529 1103 22-23 0.6552124483948703 1103 24-25 0.8351940684185593 1103 26-27 0.9303807055035023 1103 28-29 0.9107009744040084 1103 30-31 1.0419774015581211 1103 32-33 1.1014232839019371 1103 34-35 1.202720311968001 1103 36-37 1.2285667743301119 1103 38-39 0.9783349950960698 1103 40-41 1.1694820245407769 1103 42-43 1.0673210101241253 1103 44-45 1.1636383061357591 1103 46-47 1.1650788379528407 1103 48-49 1.2767775083684754 1103 50-51 1.2802005738357636 1103 52-53 1.2021625050901292 1103 54-55 1.3152241980833779 1103 56-57 1.4704874850097056 1103 58-59 1.393974736515922 1103 60-61 1.5967945657491782 1103 62-63 1.437590970783237 1103 64-65 1.3057128836994707 1103 66-67 1.4724471712620506 1103 68-69 1.508007267593058 1103 70-71 1.7244136417822773 1103 72-73 1.6269361400530684 1103 74-75 1.50948813342168 1103 76-77 1.4556472089240593 1103 78-79 1.4500673592841409 1103 80-81 1.5961218621296815 1103 82-83 1.6859648840363484 1103 84-85 1.5967391062462326 1103 86-87 1.6309359042778624 1103 88-89 1.7050208445301038 1103 90-91 1.6428689331554516 1103 92-93 1.5435217965264165 1103 94-95 1.6620805431053043 1103 96-97 1.8073859283237574 1103 98-99 1.6995784047062585 1103 100-101 1.2245824354651642 1104 1 0.5355522780396171 1104 2 0.5601767311481538 1104 3 0.5382581161425968 1104 4 0.47557378736746614 1104 5 0.47067183064253015 1104 6 0.5454772122927025 1104 7 0.6036503691065818 1104 8 0.4816588255006877 1104 9 0.6600876502484141 1104 10-11 0.5868753109667182 1104 12-13 0.500295292631975 1104 14-15 0.6017089146927646 1104 16-17 0.684466880079988 1104 18-19 0.768385372184639 1104 20-21 0.8200851599480714 1104 22-23 0.7574574790933966 1104 24-25 0.7493060983808988 1104 26-27 0.7726102975955129 1104 28-29 0.8116079925372262 1104 30-31 0.6557978133972497 1104 32-33 0.641953199775827 1104 34-35 0.639214747636899 1104 36-37 0.7985765664092455 1104 38-39 0.848429127710844 1104 40-41 0.9189191586779728 1104 42-43 0.7580878657231267 1104 44-45 0.7679918502251368 1104 46-47 0.7342023396575215 1104 48-49 0.5701539173200061 1104 50-51 0.46777964308792264 1104 52-53 0.6911910879547065 1104 54-55 0.8396221996415321 1104 56-57 0.8293421691258089 1104 58-59 0.6383621597696063 1104 60-61 0.7649074105268525 1104 62-63 0.7995905562280576 1104 64-65 0.6404697613842494 1104 66-67 0.6448735341121896 1104 68-69 0.6626292004208238 1104 70-71 0.5543495286801914 1104 72-73 0.4882459914564805 1104 74-75 0.630023624347924 1104 76-77 0.7968904455993275 1104 78-79 0.6612045984896255 1104 80-81 0.5229421524612548 1104 82-83 0.6933589759103498 1104 84-85 0.7100091603528291 1104 86-87 0.850049756853462 1104 88-89 1.0127816033090227 1104 90-91 0.7825490109202846 1104 92-93 0.7461106221201952 1104 94-95 0.36493740006232755 1104 96-97 0.5671940893427063 1104 98-99 0.49414129915908234 1104 100-101 0.24892683075067268 1105 1 0.5157557528157781 1105 2 0.6096610569539855 1105 3 0.5926495753993279 1105 4 0.5281765096610016 1105 5 0.4342391002051329 1105 6 0.5539187068276306 1105 7 0.4210764015922521 1105 8 0.40577457367054137 1105 9 0.46225024988739705 1105 10-11 0.5373285458719508 1105 12-13 0.5418890762952984 1105 14-15 0.6845872588741884 1105 16-17 0.6512817359924057 1105 18-19 0.6013993731545284 1105 20-21 0.6527906810976063 1105 22-23 0.6584344161013718 1105 24-25 0.7181753555644548 1105 26-27 0.8261302827765817 1105 28-29 0.7951564292209596 1105 30-31 0.6400082552087341 1105 32-33 0.7328613851800725 1105 34-35 0.8953404135444814 1105 36-37 0.7654049508342737 1105 38-39 0.6914616612563549 1105 40-41 0.7339851347066357 1105 42-43 0.7731894024134931 1105 44-45 0.7736825967522449 1105 46-47 0.6425151497912367 1105 48-49 0.629914412100824 1105 50-51 0.5805140766015242 1105 52-53 0.7021765375190725 1105 54-55 0.7345802865255067 1105 56-57 0.9087713372531141 1105 58-59 0.8094629079409614 1105 60-61 0.8611273954403345 1105 62-63 0.8487583743049463 1105 64-65 0.6826684628254398 1105 66-67 0.7884913319260392 1105 68-69 0.5896416856026434 1105 70-71 0.655173519163256 1105 72-73 0.5203192702059845 1105 74-75 0.776805904791317 1105 76-77 0.9383371401377012 1105 78-79 0.8619235720352876 1105 80-81 0.8797006256957616 1105 82-83 1.0065061331238567 1105 84-85 1.0041444460735285 1105 86-87 1.2189774988376705 1105 88-89 1.1807578597287502 1105 90-91 1.4005310206744923 1105 92-93 1.2961503602676565 1105 94-95 1.1899887563774811 1105 96-97 0.9720056906815238 1105 98-99 1.150447057815768 1105 100-101 1.0954477879033444 1106 1 0.43527901658733725 1106 2 0.4314808033459805 1106 3 0.1697476262403299 1106 4 0.37850620241928823 1106 5 0.42288388224852724 1106 6 0.20986774877614778 1106 7 0.39882651503856437 1106 8 0.43046866566925246 1106 9 0.5618786047880278 1106 10-11 0.24669116732125218 1106 12-13 0.25761704278753683 1106 14-15 0.5089931104978831 1106 16-17 0.3225994806416139 1106 18-19 -0.1122515627811751 1106 20-21 0.24511891692004184 1106 22-23 0.43244172783712287 1106 24-25 0.4603525600180802 1106 26-27 0.21002920072086084 1106 28-29 0.2339071876128216 1106 30-31 0.35440280484527875 1106 32-33 0.6038543337164839 1106 34-35 0.3133601277627278 1106 36-37 0.2907631044254231 1106 38-39 0.3062257973425062 1106 40-41 0.554526165507859 1106 42-43 0.011939396164017069 1106 44-45 0.4214817384129361 1106 46-47 0.1944914976701142 1106 48-49 0.7154568971910535 1106 50-51 0.19299384669152175 1106 52-53 0.472751197834512 1106 54-55 0.21091728396131515 1106 56-57 -0.16547886574446125 1106 58-59 -0.6735570547857179 1106 60-61 0.20786209754047746 1106 62-63 0.3222648753738291 1106 64-65 0.47701905734878736 1106 66-67 -0.31399913431989646 1106 68-69 -0.3604742899017772 1106 70-71 -0.27554409379118283 1106 72-73 0.3400080200085718 1106 74-75 0.34520630746321146 1106 76-77 0.3802001358201288 1106 78-79 0.31688707952745077 1106 80-81 0.32673347909901906 1106 82-83 0.38248939571316143 1106 84-85 0.35685805524828496 1106 86-87 0.3539196375453777 1106 88-89 0.0772165998292138 1106 90-91 -0.06077459852765443 1106 92-93 0.1155184018476767 1106 94-95 0.1262234036293144 1106 96-97 0.11828643110137449 1106 98-99 -0.17420480245367287 1106 100-101 -0.6274988339553378 1107 1 0.44234831117016427 1107 2 0.41960863547584637 1107 3 0.3417293445021805 1107 4 0.35841373771052076 1107 5 0.3899412851501083 1107 6 0.28819817279894977 1107 7 0.21669956273240842 1107 8 0.3666639417677473 1107 9 0.3355541415278367 1107 10-11 0.46580357269213835 1107 12-13 0.37841876134209684 1107 14-15 0.4138988307581357 1107 16-17 0.3800379654393993 1107 18-19 0.3904622313681969 1107 20-21 0.38632029238352317 1107 22-23 0.47452878102742346 1107 24-25 0.2924106846276402 1107 26-27 0.4047421374295155 1107 28-29 0.4950842631832515 1107 30-31 0.4134939004611127 1107 32-33 0.2547890101057817 1107 34-35 0.36911472294768544 1107 36-37 0.32049890765483013 1107 38-39 0.5157406345392559 1107 40-41 0.32294672124307766 1107 42-43 0.4303604253855937 1107 44-45 0.4740243744233297 1107 46-47 0.47969771819020224 1107 48-49 0.6277112689765474 1107 50-51 0.5420410917327558 1107 52-53 0.40516245716757027 1107 54-55 0.5761937755939428 1107 56-57 0.4798719718528801 1107 58-59 0.5342596882079036 1107 60-61 0.5547498144151604 1107 62-63 0.7324376290183103 1107 64-65 0.5118267601967901 1107 66-67 0.6961142470067792 1107 68-69 0.531229913709975 1107 70-71 0.48631666736250523 1107 72-73 0.6196362844458143 1107 74-75 0.5824023041218318 1107 76-77 0.5391991455162675 1107 78-79 0.6793674190831602 1107 80-81 0.558661007566311 1107 82-83 0.346353348451764 1107 84-85 0.44329628723306413 1107 86-87 0.19477083398797035 1107 88-89 0.46980234892335204 1107 90-91 0.6337657308868394 1107 92-93 0.7898156118078781 1107 94-95 0.5834031640107931 1107 96-97 0.42429821545905 1107 98-99 0.35497042164167425 1107 100-101 0.07840717642290862 1108 1 0.32664558248718834 1108 2 0.2920524768799666 1108 3 0.3385558401348412 1108 4 0.43710211941605337 1108 5 0.3375225915332436 1108 6 0.2607389792880994 1108 7 0.3442889649039671 1108 8 0.3141298733882252 1108 9 0.3034712974198186 1108 10-11 0.15446198210158002 1108 12-13 0.3412863857030999 1108 14-15 0.44306224639865377 1108 16-17 0.3514639947121836 1108 18-19 0.3879904737412474 1108 20-21 0.4063355788411869 1108 22-23 0.5445858454408992 1108 24-25 0.37883615937522563 1108 26-27 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-1.2600626835872433 2316 60-61 -1.235205942285269 2316 62-63 -1.255307105571891 2316 64-65 -1.34151534465283 2316 66-67 -1.3049523350876129 2316 68-69 -1.22941283557466 2316 70-71 -1.376935250870961 2316 72-73 -1.3102030139471665 2316 74-75 -1.2947423244673146 2316 76-77 -1.2125643721189086 2316 78-79 -1.2785871604626386 2316 80-81 -1.3344613522287183 2316 82-83 -1.0175322904046276 2316 84-85 -1.017434748648725 2316 86-87 -1.4640138967164766 2316 88-89 -1.292034698018547 2316 90-91 -1.442036169989315 2316 92-93 -1.4216167882304056 2316 94-95 -1.463314446022455 2316 96-97 -1.436112531505561 2316 98-99 -1.5586242857339734 2316 100-101 -1.2769222340140534 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6517.0 3 2546.0 4 2698.0 5 3021.0 6 3690.0 7 4560.0 8 5539.0 9 7037.0 10 8733.0 11 10785.0 12 12689.0 13 14946.0 14 16637.0 15 18505.0 16 21596.0 17 24084.0 18 23386.0 19 22642.0 20 23767.0 21 25953.0 22 28277.0 23 31338.0 24 34920.0 25 38790.0 26 43173.0 27 48752.0 28 55231.0 29 62580.0 30 72190.0 31 83032.0 32 97394.0 33 116737.0 34 140955.0 35 172736.0 36 201368.0 37 196339.0 38 91434.0 39 5165.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.963164249508175 18.173748335946097 14.136957616934087 23.72612979761164 2 15.737097594774108 19.542453347275043 39.914944331542834 24.805504726408014 3 19.477902164417475 23.34132720806898 29.221322396003735 27.95944823150981 4 13.113378113692942 15.905273123463814 35.810701411548294 35.170647351294946 5 14.240190345969092 36.80233839483295 33.834254628213664 15.123216630984293 6 33.44597025935355 35.10252021842193 16.896044925828303 14.555464596396217 7 29.637873614097217 31.918496971740932 19.97408765791212 18.469541756249736 8 26.99656510367923 34.85607553961485 19.156053112456743 18.991306244249177 9 26.657549169560152 16.45657637421536 19.521280811603393 37.364593644621095 10-11 25.591914215722095 26.12770057347662 26.831814292957628 21.448570917843657 12-13 26.39949747684473 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28.21227111019793 22.880393369893678 38-39 22.588622506789445 26.19096329202712 28.266409327170372 22.954004874013055 40-41 22.92394374113052 25.999943797333184 28.39263183038035 22.68348063115595 42-43 22.735484465611005 26.05291946138047 28.193251772510553 23.01834430049797 44-45 22.66073184018673 26.350387952335957 28.148507766491942 22.840372440985366 46-47 22.941465208731316 26.62950742842918 27.595932339017455 22.833095023822054 48-49 22.977070946689185 26.574544359276437 27.509356976704 22.939027717330376 50-51 22.928050438867597 26.71478663504872 27.50913136807561 22.84803155800807 52-53 23.179917681690736 26.553119585199784 27.45193954332978 22.815023189779694 54-55 22.766354814573177 26.659490612546744 27.584847567854315 22.989307005025765 56-57 22.754898167729987 26.958613843749202 27.432043217218105 22.854444771302706 58-59 22.84395874624829 26.447649173402088 27.483769527002462 23.22462255334716 60-61 22.580994115240582 26.56624400397412 27.805066782353816 23.047695098431483 62-63 22.67482434965544 26.65021981578628 27.822964006850697 22.851991827707575 64-65 22.514170183809213 26.60493802880058 27.668312901034327 23.212578886355885 66-67 22.395787971331682 26.761075820449353 27.573897334610724 23.269238873608238 68-69 22.703492602950952 26.425393678553306 27.75743568217105 23.11367803632469 70-71 22.617112999435452 26.459193282288812 27.493366057877367 23.430327660398365 72-73 22.59278887601286 26.553056657386186 27.406564824920395 23.44758964168056 74-75 22.502436986100818 26.81867727503787 27.378853901383625 23.300031837477686 76-77 22.73106975805851 26.577823383584327 27.33151802991907 23.359588828438092 78-79 22.758381783517443 26.765207794958933 27.269235804756292 23.207174616767333 80-81 22.765104774234732 26.846192142662883 27.16690379380952 23.221799289292868 82-83 22.667115443291472 26.82225130404635 27.15678281103114 23.35385044163104 84-85 22.63877623437583 26.762104666945184 27.10225496016221 23.496864138516774 86-87 22.84522747872664 26.648358904074183 27.04027876350113 23.46613485369805 88-89 22.69921581676012 26.684459547565844 26.97681398580017 23.639510649873873 90-91 22.649984851576363 26.752622026313848 26.947246949658332 23.650146172451453 92-93 22.72010831155759 26.38188984896686 27.08646012797647 23.811541711499082 94-95 22.581771111147333 26.263262705695944 27.22650736899732 23.928458814159402 96-97 22.850731826315506 26.016222461484933 27.107378462927258 24.025667249272306 98-99 22.662678645342893 26.12552994008695 26.877274207657553 24.334517206912608 100-101 23.07526562318919 25.6289669077894 27.06695515882258 24.228812310198826 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 515.0 1 356.0 2 304.5 3 430.0 4 375.5 5 383.0 6 668.5 7 1308.0 8 2052.0 9 2356.5 10 2035.0 11 1640.0 12 1575.0 13 1617.5 14 1726.5 15 1851.0 16 1966.0 17 2081.5 18 2240.0 19 2438.0 20 2719.0 21 3171.0 22 3878.5 23 4693.0 24 5606.5 25 6910.0 26 8804.0 27 11138.0 28 13145.0 29 15204.0 30 17943.5 31 21241.5 32 25105.5 33 27604.5 34 28850.0 35 30994.5 36 34056.5 37 37493.0 38 40865.5 39 43664.0 40 46457.5 41 50723.0 42 54229.0 43 56627.0 44 60004.5 45 63454.0 46 65166.5 47 66742.5 48 68796.0 49 68929.0 50 67661.5 51 66347.5 52 65631.5 53 65721.0 54 65652.5 55 62446.5 56 56896.5 57 52063.0 58 48482.0 59 43654.5 60 36429.0 61 30223.0 62 25221.5 63 19641.0 64 14898.5 65 11421.5 66 8611.0 67 6733.5 68 5084.5 69 3863.0 70 3056.0 71 2207.5 72 1653.0 73 1264.5 74 920.0 75 708.0 76 524.5 77 327.5 78 197.5 79 118.0 80 71.0 81 39.0 82 18.5 83 17.0 84 12.0 85 7.5 86 10.0 87 7.5 88 5.0 89 4.5 90 4.5 91 3.5 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18019465742787438 2 0.050962442870932975 3 0.04944536904787323 4 0.05540128850136705 5 0.061020080438625376 6 0.06551511398843203 7 0.037477342221513006 8 0.036465959672806504 9 0.0418599999325745 10-11 0.04315232207814391 12-13 0.040427207988573625 14-15 0.03579170464033551 16-17 0.034021785180099136 18-19 0.04242187912630033 20-21 0.05160860394371768 22-23 0.04823732878136269 24-25 0.06866163747329669 26-27 0.05379993279924843 28-29 0.04573696636928274 30-31 0.05447418783171943 32-33 0.050962442870932975 34-35 0.050119624080344226 36-37 0.048124952942617524 38-39 0.02865583888001744 40-41 0.026239758346996364 42-43 0.023346080499308326 44-45 0.004354563751375199 46-47 0.010872362398594852 48-49 0.010535234882359353 50-51 0.009102442938358482 52-53 0.01047904696298677 54-55 0.011939932866673933 56-57 0.009748604011143188 58-59 0.012305154342595725 60-61 0.01292322145569414 62-63 0.003933154356080826 64-65 0.007023489921572902 66-67 0.004326469791688908 68-69 0.010170013406437563 70-71 0.025031718080485824 72-73 0.021351409361581624 74-75 0.022053758353738913 76-77 0.013569382528478847 78-79 0.004438845630434074 80-81 0.005955919453493821 82-83 0.005000724824159906 84-85 0.004916442945101031 86-87 0.009495758373966563 88-89 0.031802362364882104 90-91 0.038151597253984 92-93 0.024188899289897075 94-95 0.03365656370417735 96-97 0.03739306034245413 98-99 0.02323370466056316 100-101 0.12496193268462508 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1779742.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.54831780103179 #Duplication Level Percentage of deduplicated Percentage of total 1 80.73054979845821 48.07368435650613 2 11.034582115818544 13.141816052686888 3 3.39445319411167 6.0640193269106755 4 1.5046268393418818 3.5839198880436953 5 0.8325632388349982 2.47888701678014 6 0.5223234803285846 1.866209076092852 7 0.3542113567247656 1.476488330728669 8 0.25305161963766853 1.2055038612999762 9 0.1908631936729717 1.0229023902022198 >10 1.0666452204990473 11.578150006862192 >50 0.0761151140562332 3.0947489098929037 >100 0.03555886990065286 3.7139846372075245 >500 0.003318267053618238 1.2646222147683346 >1k 0.0010428839311371605 1.1010681720815547 >5k 9.480763010337824E-5 0.3339957599361369 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5916 0.3324077310082023 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4818 0.27071339553710594 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3245 0.18232979836403254 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.618791937258322E-5 2 0.0 0.0 0.0 0.0 5.618791937258322E-5 3 0.0 0.0 0.0 0.0 5.618791937258322E-5 4 0.0 0.0 0.0 0.0 5.618791937258322E-5 5 0.0 0.0 0.0 0.0 5.618791937258322E-5 6 0.0 0.0 0.0 0.0 5.618791937258322E-5 7 0.0 0.0 0.0 0.0 5.618791937258322E-5 8 0.0 0.0 0.0 0.0 5.618791937258322E-5 9 0.0 0.0 0.0 0.0 5.618791937258322E-5 10-11 0.0 0.0 0.0 0.0 5.618791937258322E-5 12-13 0.0 0.0 0.0 0.0 5.618791937258322E-5 14-15 5.618791937258322E-5 0.0 0.0 0.0 8.428187905887483E-5 16-17 5.618791937258322E-5 0.0 0.0 0.0 1.1237583874516644E-4 18-19 8.428187905887483E-5 0.0 0.0 5.618791937258322E-5 1.1237583874516644E-4 20-21 1.1237583874516644E-4 0.0 0.0 5.618791937258322E-5 1.1237583874516644E-4 22-23 1.1237583874516644E-4 0.0 0.0 1.6856375811774964E-4 1.1237583874516644E-4 24-25 1.1237583874516644E-4 0.0 0.0 4.775973146669574E-4 1.1237583874516644E-4 26-27 1.1237583874516644E-4 0.0 0.0 0.001011382548706498 2.2475167749033288E-4 28-29 2.809395968629161E-4 0.0 0.0 0.0032588993236098264 2.2475167749033288E-4 30-31 2.809395968629161E-4 0.0 0.0 0.009186724817417356 2.2475167749033288E-4 32-33 3.0903355654920766E-4 0.0 0.0 0.02253135566840587 2.2475167749033288E-4 34-35 3.371275162354993E-4 0.0 0.0 0.04275900664253583 3.371275162354993E-4 36-37 3.371275162354993E-4 0.0 0.0 0.07916877839596975 3.371275162354993E-4 38-39 3.371275162354993E-4 0.0 0.0 0.13642426823663206 3.371275162354993E-4 40-41 3.371275162354993E-4 0.0 0.0 0.19764100639306148 3.371275162354993E-4 42-43 3.371275162354993E-4 0.0 0.0 0.2613300130018845 3.652214759217909E-4 44-45 3.371275162354993E-4 0.0 0.0 0.3416506437449922 4.2140939529437414E-4 46-47 4.2140939529437414E-4 0.0 0.0 0.4354001872181473 4.4950335498066576E-4 48-49 4.4950335498066576E-4 0.0 0.0 0.5392635561783674 4.4950335498066576E-4 50-51 4.4950335498066576E-4 0.0 0.0 0.6507403882135725 4.4950335498066576E-4 52-53 4.4950335498066576E-4 0.0 0.0 0.7597168578366977 4.775973146669574E-4 54-55 4.4950335498066576E-4 0.0 0.0 0.8800151932133984 5.05691274353249E-4 56-57 4.4950335498066576E-4 0.0 0.0 1.0275365755261157 5.05691274353249E-4 58-59 5.05691274353249E-4 0.0 0.0 1.1868574209070752 5.05691274353249E-4 60-61 5.05691274353249E-4 0.0 0.0 1.3563482796944726 5.05691274353249E-4 62-63 5.05691274353249E-4 0.0 0.0 1.5247715680137908 5.618791937258322E-4 64-65 5.05691274353249E-4 0.0 0.0 1.703224399941115 5.618791937258322E-4 66-67 5.618791937258322E-4 0.0 0.0 1.9109792318212415 5.618791937258322E-4 68-69 5.618791937258322E-4 0.0 0.0 2.147474184460444 5.618791937258322E-4 70-71 6.180671130984153E-4 0.0 0.0 2.4002355397580097 6.180671130984154E-4 72-73 7.023489921572901E-4 0.0 0.0 2.651198881635653 7.585369115298735E-4 74-75 7.304429518435818E-4 0.0 0.0 2.9094104651123587 8.990067099613315E-4 76-77 7.304429518435818E-4 5.618791937258322E-5 0.0 3.1883553908375486 8.990067099613315E-4 78-79 7.304429518435818E-4 5.618791937258322E-5 0.0 3.5069689876397816 9.271006696476231E-4 80-81 8.990067099613315E-4 5.618791937258322E-5 0.0 3.8489286649413232 9.551946293339147E-4 82-83 8.990067099613315E-4 5.618791937258322E-5 0.0 4.194034865727729 0.0011237583874516644 84-85 8.990067099613315E-4 5.618791937258322E-5 0.0 4.547850194016886 0.001151852347137956 86-87 8.990067099613315E-4 5.618791937258322E-5 0.0 4.906020086057417 0.001292322145569414 88-89 8.990067099613315E-4 5.618791937258322E-5 0.0 5.2980993874393025 0.0014046979843145805 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2985 0.0 56.2886 1 GTATCAA 5170 0.0 41.79799 1 ATCAACG 6650 0.0 31.940573 3 TATCAAC 6685 0.0 31.702265 2 TCAACGC 6770 0.0 31.374418 4 CAACGCA 6925 0.0 30.808544 5 AACGCAG 7055 0.0 30.168406 6 ACGCAGA 8160 0.0 26.021952 7 CGCAGAG 8305 0.0 25.567627 8 TACATGG 6105 0.0 24.206472 2 GTACATG 6230 0.0 24.06648 1 ACATGGG 6155 0.0 23.083408 3 GTGTAAG 700 0.0 22.439127 1 GAGTACT 5370 0.0 22.334333 12-13 GCAGAGT 9350 0.0 22.149963 9 GTATAGG 435 0.0 21.884207 1 TGGTATC 1450 0.0 21.628815 2 GTGGTAT 1570 0.0 20.918932 1 CAGAGTA 8850 0.0 20.743984 10-11 TAAGGTG 875 0.0 20.635746 5 >>END_MODULE