FastQCFastQC Report
Thu 26 May 2016
SRR938506_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938506_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1779742
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58050.32617087195784555No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50830.2856031941708405No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35480.19935473793392527No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30600.057.0371061
GTATCAA52950.040.5961951
ATCAACG70150.030.6303943
TCAACGC71250.030.0907844
TATCAAC71300.030.0815262
CAACGCA72900.029.409725
AACGCAG75000.028.497226
TAACGCA1351.3349467E-524.6493844
TGGTATC15050.024.647392
ACGCAGA87200.024.4557557
CGCAGAG88100.024.0441918
GTGGTAT16900.022.5121061
GCAGAGT98700.021.3175759
GAGTACT59150.021.15812112-13
GTAAGGT7900.021.0611864
TACATGG58500.020.973782
TAAGGTG8000.020.797925
GTACATG60200.020.7764871
AGAGTAC89450.020.22999810-11
CAGAGTA93450.020.12644810-11
Produced by FastQC (version 0.11.5)