##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938506_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1779742 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.775141003583666 31.0 31.0 34.0 30.0 34.0 2 32.00033712751623 31.0 31.0 34.0 30.0 34.0 3 32.066441090899694 33.0 31.0 34.0 30.0 34.0 4 35.742981847930764 37.0 35.0 37.0 33.0 37.0 5 35.60679075955953 37.0 35.0 37.0 33.0 37.0 6 35.63904374903778 37.0 35.0 37.0 33.0 37.0 7 35.57351177867354 37.0 35.0 37.0 33.0 37.0 8 35.562399493859225 37.0 35.0 37.0 33.0 37.0 9 37.13681926930982 39.0 37.0 39.0 33.0 39.0 10-11 37.04753919388316 39.0 37.0 39.0 33.0 39.0 12-13 36.935684498090176 39.0 37.0 39.0 33.0 39.0 14-15 37.98083851479596 40.0 37.0 41.0 33.0 41.0 16-17 37.902280499083574 40.0 37.0 41.0 33.0 41.0 18-19 38.00555979462192 40.0 37.0 41.0 33.0 41.0 20-21 38.05412385615443 40.0 37.0 41.0 33.0 41.0 22-23 37.95722526074004 40.0 37.0 41.0 33.0 41.0 24-25 37.851725699567695 40.0 37.0 41.0 32.0 41.0 26-27 37.66226565423528 40.0 37.0 41.0 32.0 41.0 28-29 37.53243166706186 39.5 36.5 41.0 32.0 41.0 30-31 37.29180465483199 39.0 36.0 41.0 31.0 41.0 32-33 37.0770889825604 39.0 36.0 41.0 31.0 41.0 34-35 36.92087476724154 39.0 35.5 40.5 30.5 41.0 36-37 36.92414968012218 39.0 36.0 41.0 30.5 41.0 38-39 36.80706697937117 39.0 35.0 41.0 30.0 41.0 40-41 36.620453975913364 39.0 35.0 40.5 30.0 41.0 42-43 36.40350820512187 39.0 35.0 40.0 30.0 41.0 44-45 36.13904936782972 38.5 35.0 40.0 29.5 41.0 46-47 35.770985064127274 38.0 35.0 40.0 28.5 41.0 48-49 35.40796868310126 38.0 34.0 40.0 27.0 41.0 50-51 35.028424063712606 38.0 34.0 40.0 26.0 41.0 52-53 34.53056594719909 38.0 33.0 40.0 24.5 41.0 54-55 34.21627994394693 37.5 33.0 40.0 24.0 41.0 56-57 33.86662252169134 37.0 33.0 40.0 23.0 41.0 58-59 33.40377509773889 36.5 32.0 39.0 22.5 41.0 60-61 33.02735031257339 36.0 31.5 39.0 22.0 40.0 62-63 32.56090461426432 35.5 31.0 39.0 20.5 40.0 64-65 32.046885447441255 35.0 31.0 38.5 19.0 40.0 66-67 31.545054002209312 35.0 30.0 38.0 17.5 40.0 68-69 31.002991725766993 34.5 29.5 37.5 14.0 39.5 70-71 30.411887509537898 34.0 29.0 37.0 10.0 39.0 72-73 29.959854855366675 34.0 29.0 36.0 8.0 39.0 74-75 29.43912797472892 34.0 28.0 36.0 7.0 38.5 76-77 28.192392211904874 31.5 26.5 34.5 6.5 36.5 78-79 28.885047383272408 33.0 28.0 35.0 2.0 37.0 80-81 28.606157240768606 33.0 27.5 35.0 2.0 37.0 82-83 28.127541801002614 33.0 26.5 35.0 2.0 36.0 84-85 27.592794910723015 33.0 26.0 35.0 2.0 36.0 86-87 27.00874424495236 32.0 25.5 35.0 2.0 35.0 88-89 26.3573936559344 32.0 24.5 34.0 2.0 35.0 90-91 25.808664682858527 32.0 23.5 34.0 2.0 35.0 92-93 25.043091920064818 31.0 20.0 34.0 2.0 35.0 94-95 24.258786666831483 31.0 15.5 34.0 2.0 35.0 96-97 23.242032553032967 30.5 2.0 34.0 2.0 35.0 98-99 21.984902587004186 30.0 2.0 34.0 2.0 35.0 100-101 18.38541429038591 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3579504091163592 1101 2 0.4111684256587047 1101 3 0.3497629877369448 1101 4 0.3623850910864306 1101 5 0.1838402100030052 1101 6 0.2224913129639745 1101 7 0.33145824912109134 1101 8 0.33013431846817554 1101 9 0.391781457807312 1101 10-11 0.41093681599092946 1101 12-13 0.4684400088548273 1101 14-15 0.6544358503131562 1101 16-17 0.5790411494321503 1101 18-19 0.5311873499583797 1101 20-21 0.5169770969309795 1101 22-23 0.5060080199991646 1101 24-25 0.5468531766511404 1101 26-27 0.6062185645016172 1101 28-29 0.5284514182039075 1101 30-31 0.5562417536581918 1101 32-33 0.645715781456154 1101 34-35 0.36831539269324054 1101 36-37 0.6899897237530652 1101 38-39 0.6557606720467106 1101 40-41 0.836058452010775 1101 42-43 0.7808543859523596 1101 44-45 0.6922515048051068 1101 46-47 0.8274013123374928 1101 48-49 0.8751960841475039 1101 50-51 0.9365780371515697 1101 52-53 0.9093631474253172 1101 54-55 0.959300069458564 1101 56-57 0.9215541912007197 1101 58-59 0.9522866470953133 1101 60-61 0.8323686535317805 1101 62-63 1.0296859507432643 1101 64-65 1.1134383287317462 1101 66-67 0.9082684192529022 1101 68-69 0.9941940536153808 1101 70-71 0.7154089383229767 1101 72-73 0.8028237583076994 1101 74-75 0.9798170126505532 1101 76-77 0.9482222261062425 1101 78-79 0.9596040879730232 1101 80-81 0.842404417024202 1101 82-83 0.9390406689259478 1101 84-85 0.7617695134953983 1101 86-87 0.8723130971825803 1101 88-89 0.7398760589242919 1101 90-91 0.8664982907227561 1101 92-93 0.6652198897013086 1101 94-95 0.8524533455224734 1101 96-97 0.7073383092139203 1101 98-99 0.36505900285820303 1101 100-101 0.15613949278155204 1102 1 0.2925697776180307 1102 2 0.2642362602019155 1102 3 0.35313637482388316 1102 4 0.2417120219270501 1102 5 0.12033840615050195 1102 6 0.25454903624407166 1102 7 0.21245902062354816 1102 8 0.2807199320070737 1102 9 0.2810051075049884 1102 10-11 0.3748811874009732 1102 12-13 0.3616104818968964 1102 14-15 0.453460777982734 1102 16-17 0.4234614663446621 1102 18-19 0.4271468983147102 1102 20-21 0.4705529828158461 1102 22-23 0.5008751063728383 1102 24-25 0.5267995504742373 1102 26-27 0.5289921899930334 1102 28-29 0.4274897260528405 1102 30-31 0.3780119975234939 1102 32-33 0.384528419068495 1102 34-35 0.19531329899177763 1102 36-37 0.2175214201126323 1102 38-39 0.4037563640244386 1102 40-41 0.5453144052843015 1102 42-43 0.3656789556527045 1102 44-45 0.44985587926689874 1102 46-47 0.4856593382205645 1102 48-49 0.6597597073945991 1102 50-51 0.5882310001197837 1102 52-53 0.4237657899777787 1102 54-55 0.4601916969266924 1102 56-57 0.2898897660985753 1102 58-59 0.4512538027097648 1102 60-61 0.46700934463451915 1102 62-63 0.5940459316155398 1102 64-65 0.6265858379586362 1102 66-67 0.6973214881420411 1102 68-69 0.7452685050856971 1102 70-71 0.7435443905582098 1102 72-73 0.6193262980700673 1102 74-75 0.5502536052659437 1102 76-77 0.5418821632457664 1102 78-79 0.59996810135452 1102 80-81 0.8144129829754689 1102 82-83 0.585803439994045 1102 84-85 0.4583983344676099 1102 86-87 0.2928071487700912 1102 88-89 0.5578949091582288 1102 90-91 0.33647989132835576 1102 92-93 0.3737608602388782 1102 94-95 0.43331164777545084 1102 96-97 0.5569665148956169 1102 98-99 0.477410984624246 1102 100-101 0.48242735976015894 1103 1 0.28698398976998973 1103 2 0.27461597229650536 1103 3 0.3053208610461269 1103 4 0.17919304082154497 1103 5 0.051387782895361056 1103 6 0.11766011536069243 1103 7 0.2480478904407235 1103 8 0.1498194796099952 1103 9 0.3026058867707633 1103 10-11 0.31546554674508087 1103 12-13 0.3088356973535369 1103 14-15 0.40497263006621864 1103 16-17 0.45689633218902514 1103 18-19 0.5093511136386155 1103 20-21 0.5333340437780905 1103 22-23 0.43708621900868394 1103 24-25 0.5574628937133639 1103 26-27 0.6379667941174745 1103 28-29 0.6224445200895588 1103 30-31 0.6349244943459169 1103 32-33 0.6035574413194169 1103 34-35 0.5686646142277283 1103 36-37 0.4962652814825077 1103 38-39 0.4750915765340835 1103 40-41 0.6266693918878516 1103 42-43 0.7082893160181385 1103 44-45 0.6280105671219118 1103 46-47 0.7161197664118006 1103 48-49 0.7783401569121793 1103 50-51 0.7318158926220448 1103 52-53 0.74454928502076 1103 54-55 0.6977327804750431 1103 56-57 0.606201031483117 1103 58-59 0.701640173657367 1103 60-61 0.7323837588916646 1103 62-63 0.768073705331517 1103 64-65 0.6652623009378829 1103 66-67 0.7979938973195608 1103 68-69 0.858208051248873 1103 70-71 0.4926552312159025 1103 72-73 0.3676868929575967 1103 74-75 0.6301710894792869 1103 76-77 0.5800434649615447 1103 78-79 0.5848113371071619 1103 80-81 0.5859963277443327 1103 82-83 0.3903852257814435 1103 84-85 0.28537163831602186 1103 86-87 0.4566261809418002 1103 88-89 0.5082065929706943 1103 90-91 0.3919328395580237 1103 92-93 0.35454579503386086 1103 94-95 0.40464596044009227 1103 96-97 0.5001682983884095 1103 98-99 0.7966928633206436 1103 100-101 0.5462893595307001 1104 1 0.28220945775274586 1104 2 0.24084402052785592 1104 3 0.1916898580370301 1104 4 0.09487394769672619 1104 5 0.07832672177706002 1104 6 0.18505392133457121 1104 7 0.14860468092597046 1104 8 0.13890524249872982 1104 9 0.22507692151477698 1104 10-11 0.27992458797902486 1104 12-13 0.13541940533514918 1104 14-15 0.42034690906185546 1104 16-17 0.42710911335392154 1104 18-19 0.3160540419706024 1104 20-21 0.31488490898333765 1104 22-23 0.41902090512608936 1104 24-25 0.39236629521005284 1104 26-27 0.4947379811032988 1104 28-29 0.33683387316906277 1104 30-31 0.3413551028128481 1104 32-33 0.39188056563593676 1104 34-35 0.3881865892257679 1104 36-37 0.3931375341610348 1104 38-39 0.4646133371070249 1104 40-41 0.3454080005549116 1104 42-43 0.5577450336631742 1104 44-45 0.5094406395261188 1104 46-47 0.2961289152156681 1104 48-49 0.35919985857539416 1104 50-51 0.4254953557016208 1104 52-53 0.43215694428499773 1104 54-55 0.30696092162393285 1104 56-57 0.1884759387442685 1104 58-59 0.14576814255143233 1104 60-61 0.176447400258553 1104 62-63 0.11980232893686349 1104 64-65 0.10699943007182355 1104 66-67 -0.026735033628416005 1104 68-69 -0.016860743467695727 1104 70-71 -0.24094660998087392 1104 72-73 -0.4331229191345507 1104 74-75 -0.3439192120738639 1104 76-77 -0.388771979362982 1104 78-79 -0.3805947482769092 1104 80-81 -0.24457568692234588 1104 82-83 -0.49239215094199906 1104 84-85 -0.3538438517525222 1104 86-87 -0.23174626114555608 1104 88-89 -0.33564548230680913 1104 90-91 -0.5535495811583004 1104 92-93 -0.5058205859709375 1104 94-95 -0.5662565547662197 1104 96-97 -0.5648739790919528 1104 98-99 -0.5872209663829508 1104 100-101 -0.7814701126976793 1105 1 0.2509749467846909 1105 2 0.22410166852309388 1105 3 0.2363639799547954 1105 4 0.20166475556300867 1105 5 0.12904080424508635 1105 6 0.2013559276873238 1105 7 0.1521295274516845 1105 8 0.2642993905101747 1105 9 0.19263465630062626 1105 10-11 0.14125614446859913 1105 12-13 0.32427045403274946 1105 14-15 0.3970427824492404 1105 16-17 0.33389736418265414 1105 18-19 0.24793352106042477 1105 20-21 0.28248531061662874 1105 22-23 0.23969615927629206 1105 24-25 0.4279977214393682 1105 26-27 0.22438611787032414 1105 28-29 0.26440816328638306 1105 30-31 0.38207354711121155 1105 32-33 0.3502472370896115 1105 34-35 0.26030854683424565 1105 36-37 0.4617757065095276 1105 38-39 0.41029561567724215 1105 40-41 0.2458041035107641 1105 42-43 0.26541335140304767 1105 44-45 0.3906262545843333 1105 46-47 0.022113352371569306 1105 48-49 -0.0565109666430601 1105 50-51 -0.10308831012092412 1105 52-53 0.11038736726360554 1105 54-55 0.15222744372878338 1105 56-57 0.16716080081439344 1105 58-59 0.17348196961303586 1105 60-61 0.06166629605242946 1105 62-63 0.12480535129576964 1105 64-65 -0.08354669665555292 1105 66-67 -0.016179374502929278 1105 68-69 -0.00839754384661262 1105 70-71 0.11104759684243248 1105 72-73 0.05806912717042678 1105 74-75 0.08865691108257678 1105 76-77 0.16825649919670482 1105 78-79 0.21148283581022653 1105 80-81 0.36811236311947226 1105 82-83 0.26794236903515767 1105 84-85 0.10594613815907294 1105 86-87 0.22364027224593386 1105 88-89 0.2108310537517326 1105 90-91 0.09091664766028629 1105 92-93 0.11950413549780237 1105 94-95 0.19602324639140534 1105 96-97 -0.025476423084654698 1105 98-99 -0.07992746277470175 1105 100-101 -0.30169077909139475 1106 1 0.1308389112088726 1106 2 0.17350593939787373 1106 3 0.16616608195383265 1106 4 0.13370637440282707 1106 5 0.10031646421143847 1106 6 -0.03506999753604845 1106 7 0.16907427358634664 1106 8 0.15683875556965177 1106 9 0.07587245358067918 1106 10-11 0.11755138072365412 1106 12-13 0.03354720037798131 1106 14-15 0.1507622478943702 1106 16-17 -0.04469111967179629 1106 18-19 0.18528101555133247 1106 20-21 0.1881368041523146 1106 22-23 0.28668234014867267 1106 24-25 0.23518987904253663 1106 26-27 0.20546477387788542 1106 28-29 0.2524059411210189 1106 30-31 0.27187476094992036 1106 32-33 0.41128134979833675 1106 34-35 0.2489883259234773 1106 36-37 0.006285331726452625 1106 38-39 0.1121229908370367 1106 40-41 0.4688455703045946 1106 42-43 0.45013481756164 1106 44-45 0.3931623640362716 1106 46-47 0.16520803820250052 1106 48-49 0.2758059818968732 1106 50-51 0.23117009218452722 1106 52-53 0.08153877301258916 1106 54-55 -0.3559362789216891 1106 56-57 -0.01923302417743855 1106 58-59 -0.041718394356244914 1106 60-61 -0.5257876453354555 1106 62-63 -0.33560431412364267 1106 64-65 -0.7204208781584711 1106 66-67 -0.5895489165445262 1106 68-69 -1.2623808105532177 1106 70-71 -0.7487946752951835 1106 72-73 -0.34078999854455105 1106 74-75 -0.1062239916176928 1106 76-77 -0.3185777003870349 1106 78-79 -0.17234741146007693 1106 80-81 -0.8824169304372163 1106 82-83 -0.3457562687563005 1106 84-85 -0.22584703106458193 1106 86-87 0.07470622638451019 1106 88-89 -0.07461308669876843 1106 90-91 -0.05722981762337653 1106 92-93 -0.49265139725575935 1106 94-95 -0.5802724015936036 1106 96-97 -0.5851196237909484 1106 98-99 -0.6086432741165098 1106 100-101 -0.6406005534722041 1107 1 0.0859676148405697 1107 2 0.1328839677376088 1107 3 0.057504983677873156 1107 4 0.12457382720987198 1107 5 -0.047882752441275045 1107 6 0.14685120343193603 1107 7 0.043391484757528076 1107 8 0.12297242966010913 1107 9 0.1827182339134339 1107 10-11 0.15809099445635866 1107 12-13 0.1678089878204858 1107 14-15 0.30205060423387664 1107 16-17 0.3897932924403875 1107 18-19 0.2196280018394603 1107 20-21 0.2714768571655739 1107 22-23 0.34157031184509634 1107 24-25 0.4235037195648772 1107 26-27 0.41615098759125146 1107 28-29 0.3864509292106817 1107 30-31 0.24117505431058106 1107 32-33 0.2925431708153283 1107 34-35 0.3287090780264066 1107 36-37 0.33484023132009355 1107 38-39 0.19097123674232108 1107 40-41 0.22944556645931158 1107 42-43 0.2771863262756469 1107 44-45 0.2387960595053542 1107 46-47 0.06391668311615462 1107 48-49 0.06464097305416061 1107 50-51 -0.05257635559179619 1107 52-53 0.2084656830780247 1107 54-55 0.18889627765491213 1107 56-57 0.17384997166535499 1107 58-59 0.0038856579981469963 1107 60-61 0.13795340182694105 1107 62-63 0.26485872682373923 1107 64-65 0.27153057141279646 1107 66-67 0.09316142832858176 1107 68-69 0.06770359340925935 1107 70-71 -0.015151978420281154 1107 72-73 0.054469226619442423 1107 74-75 0.07995241676743348 1107 76-77 0.005422834509051455 1107 78-79 0.2536706343957853 1107 80-81 0.16509355011124782 1107 82-83 0.0218305492691222 1107 84-85 0.1664622307013346 1107 86-87 0.05044292091608682 1107 88-89 0.19206460885181187 1107 90-91 0.25111969794270195 1107 92-93 0.25525859098169335 1107 94-95 -0.04859099993628746 1107 96-97 0.14716344987578012 1107 98-99 -0.00467542986539371 1107 100-101 -0.5091403703736006 1108 1 0.1276058917729621 1108 2 0.14998541288333556 1108 3 0.08299610163697224 1108 4 0.08622163582968057 1108 5 0.052562571793934865 1108 6 -0.045622674520565454 1108 7 0.019621769431481084 1108 8 0.061959573335769846 1108 9 0.13667727838691235 1108 10-11 0.13870649786326794 1108 12-13 0.04427613995510882 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-0.3788596856190125 2316 56-57 -0.3843077527690397 2316 58-59 -0.16972122092778363 2316 60-61 -0.1392209413450658 2316 62-63 -0.21830867627885908 2316 64-65 0.1175904605831164 2316 66-67 -0.13196253719679163 2316 68-69 -0.10744530093682414 2316 70-71 -0.14288897007591217 2316 72-73 -0.13899727947419294 2316 74-75 0.3013949169067196 2316 76-77 0.13086251747731126 2316 78-79 0.05695023862039861 2316 80-81 -3.4810430529574887E-4 2316 82-83 -0.022192605248136488 2316 84-85 0.06479149970831344 2316 86-87 0.3999255585996089 2316 88-89 0.8258846613380513 2316 90-91 0.3279328946460929 2316 92-93 0.18635747339908804 2316 94-95 0.4155613558729847 2316 96-97 0.7170947206170588 2316 98-99 0.9402620195090989 2316 100-101 0.8302308804817642 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 12.0 4 40.0 5 61.0 6 138.0 7 350.0 8 824.0 9 1449.0 10 2254.0 11 3565.0 12 4936.0 13 7109.0 14 9104.0 15 12122.0 16 15142.0 17 18198.0 18 17954.0 19 15624.0 20 15743.0 21 16746.0 22 18120.0 23 20391.0 24 23631.0 25 27471.0 26 33088.0 27 39610.0 28 47275.0 29 56652.0 30 66881.0 31 79369.0 32 95431.0 33 116850.0 34 148358.0 35 192253.0 36 249685.0 37 279479.0 38 137719.0 39 6104.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.68645092994391 19.091143549221766 14.795662940073528 23.426742580760802 2 16.239738575759567 20.400017764076203 38.84216449646995 24.518079163694274 3 19.60547881781869 23.639299735296483 29.679720541408876 27.075500905475952 4 13.61815364249425 16.77040829513491 35.504471996502865 34.10696606586797 5 14.161444505243907 36.97543358046159 33.936897656508776 14.926224257785723 6 33.41428139584277 35.608419647342146 16.620779865845726 14.356519090969366 7 29.554058959107554 32.04470086113605 19.92968643769715 18.471553742059243 8 26.905191876125865 35.48295202338316 18.92914815742956 18.68270794306141 9 26.32758006497571 16.554253369308586 19.494567190075866 37.62359937563984 10-11 25.578314160142313 26.38978009172116 26.758288560926246 21.27361718721028 12-13 26.25712041408249 23.360886016063002 27.467998170521348 22.91399539933316 14-15 22.728266231847087 25.07065630861102 25.469534348236994 26.731543111304894 16-17 22.618222191755883 28.34857524292847 26.357781071638474 22.675421493677174 18-19 22.691996929892085 26.58076282966857 28.547227631870236 22.180012608569108 20-21 23.12627335872278 25.774213341034823 28.87384800718306 22.225665293059333 22-23 22.96686823146276 25.637957636556308 28.775968651636024 22.619205480344903 24-25 22.88279991695409 25.936029366111928 28.76186585838313 22.41930485855085 26-27 22.429828029115946 26.322415835920328 28.60259573285637 22.645160402107358 28-29 22.506043010728522 26.534407796186187 28.65811449075203 22.30143470233326 30-31 22.54492505093435 26.156094535050585 28.499271242685737 22.799709171329326 32-33 22.353380433793212 26.15173997129921 28.551638383540983 22.94324121136659 34-35 23.00099828916894 26.241883807907023 28.30350688055286 22.453611022371174 36-37 23.021558480014363 25.881835020330215 28.337502012231557 22.75910448742387 38-39 22.564564765364747 26.19532929343784 28.43535572535641 22.804750215841 40-41 22.903313627987306 25.99816085934451 28.48234933501832 22.616176177649862 42-43 22.738459256362454 26.08434499909537 28.233453463374946 22.94374228116723 44-45 22.653873426597787 26.359129581703417 28.210774370667206 22.77622262103159 46-47 22.92885710400721 26.653694749014186 27.678028613136064 22.739419533842543 48-49 22.925092513409247 26.617594010817296 27.60976029109837 22.847553184675082 50-51 22.974228848900573 26.732301648216428 27.56205674755105 22.731412755331952 52-53 23.29978165374532 26.546038695496314 27.44448352626392 22.70969612449445 54-55 22.884468647702867 26.646109379898885 27.533148063033856 22.93627390936439 56-57 22.791786674697793 26.87903639965793 27.48137089533202 22.84780603031226 58-59 22.906606687935668 26.487575165389142 27.48547261344622 23.120345533228974 60-61 22.605242782380817 26.619532494035653 27.761945270719014 23.013279452864516 62-63 22.609794003849995 26.73651574216937 27.791640586107423 22.862049667873208 64-65 22.46513820542528 26.664595205372464 27.662015056114875 23.208251533087378 66-67 22.333486913818874 26.923033701551912 27.55669367435693 23.186785710272286 68-69 22.668107343942314 26.461375773752984 27.751017927835214 23.119498954469485 70-71 22.579996280353466 26.57577880801623 27.431578450548088 23.412646461082215 72-73 22.592915881323215 26.719779248840354 27.354337694547215 23.33296717528922 74-75 22.564366071037263 26.9776742921165 27.13789414420742 23.32006549263882 76-77 22.620188768933925 26.703730091215466 27.392004009569927 23.284077130280682 78-79 22.66718996348909 26.894628547283823 27.319886815055217 23.118294674171874 80-81 22.66713041054819 26.9278616050408 27.225773970932472 23.17923401347854 82-83 22.654881726375194 26.958036869658248 27.18456012123194 23.20252128273462 84-85 22.584529593856743 26.89511323338445 27.162863563085406 23.3574936096734 86-87 22.72250046847823 26.853986784840238 27.06346046816357 23.360052278517962 88-89 22.555017119059144 26.937788437253385 26.94475574121298 23.562438702474488 90-91 22.473482488075057 26.960402276148482 27.086801901248155 23.47931333452831 92-93 22.543577030569942 26.683268510475234 27.10524452695421 23.667909932000615 94-95 22.414322529423515 26.54070829949181 27.26851062359337 23.77645854749131 96-97 22.606394634727955 26.330136615307165 27.178911325349404 23.884557424615476 98-99 22.35954557432795 26.5090257514998 26.959287896384925 24.17214077778733 100-101 22.438814165199226 26.363371769616045 27.246477298394932 23.951336766789794 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 504.0 1 364.5 2 312.0 3 437.5 4 366.5 5 320.5 6 647.0 7 1399.0 8 2121.0 9 2338.0 10 2006.5 11 1556.5 12 1491.0 13 1568.0 14 1688.5 15 1870.5 16 1962.0 17 2069.5 18 2194.0 19 2433.5 20 2767.0 21 3116.5 22 3742.0 23 4593.0 24 5555.5 25 6863.5 26 8731.0 27 11088.0 28 13186.0 29 15412.0 30 18201.0 31 21499.5 32 25839.0 33 28165.5 34 29027.5 35 31310.5 36 34505.5 37 37912.0 38 41307.5 39 43705.5 40 46300.5 41 51184.0 42 54913.0 43 57775.0 44 61134.0 45 64807.0 46 66431.0 47 67791.5 48 69970.5 49 69982.0 50 68862.0 51 67113.0 52 65696.0 53 65669.0 54 66317.0 55 62642.0 56 56513.5 57 51668.5 58 47658.0 59 42480.5 60 34982.5 61 28688.0 62 24082.0 63 18559.5 64 13627.0 65 10356.0 66 7556.0 67 5944.5 68 4555.5 69 3317.0 70 2567.5 71 1814.5 72 1352.5 73 1082.5 74 820.5 75 622.0 76 429.0 77 237.0 78 136.0 79 74.5 80 45.0 81 26.5 82 11.5 83 9.0 84 5.5 85 2.0 86 1.5 87 2.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.180671130984154E-4 2 0.048939677773519985 3 8.428187905887483E-4 4 0.0 5 0.08894547636679924 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.966577178040413E-4 26-27 0.009411476494907688 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011097114076085187 36-37 8.428187905887482E-5 38-39 0.004242187912630033 40-41 0.005984013413180113 42-43 2.2475167749033285E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.1237583874516643E-4 68-69 0.0013204161052557057 70-71 1.6856375811774964E-4 72-73 2.8093959686291607E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0013485100649419971 82-83 7.304429518435818E-4 84-85 0.0055626040178857385 86-87 0.00244417449270737 88-89 2.8093959686291607E-5 90-91 0.003876966436708242 92-93 0.0011237583874516644 94-95 2.8093959686291607E-5 96-97 0.0 98-99 5.6187919372583213E-5 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1779742.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.88613508746264 #Duplication Level Percentage of deduplicated Percentage of total 1 78.9055715472149 44.88633002188316 2 12.026554989559257 13.682884635457315 3 3.7155544984692637 6.340906053742562 4 1.6447783178120223 3.7426032630393706 5 0.9130952632012995 2.597123024509569 6 0.5747490455008253 1.9617151106250095 7 0.3963794471303921 1.5783946342747268 8 0.2788379481850434 1.268961055037223 9 0.22040789372627373 1.1284337895170327 >10 1.1890808869780132 12.363212395648599 >50 0.08769841995792466 3.372254168653057 >100 0.04284022427693937 4.343989786384819 >500 0.003264446524454862 1.2773466460326663 >1k 9.892262195317764E-4 0.8431421999871113 >5k 1.9784524390635525E-4 0.6127032152078801 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5805 0.32617087195784555 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5083 0.2856031941708405 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3548 0.19935473793392527 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 5.618791937258322E-5 0.0 0.0 0.0 0.0 3 5.618791937258322E-5 0.0 0.0 0.0 0.0 4 5.618791937258322E-5 0.0 0.0 0.0 0.0 5 5.618791937258322E-5 0.0 0.0 0.0 0.0 6 5.618791937258322E-5 0.0 0.0 0.0 0.0 7 5.618791937258322E-5 0.0 0.0 0.0 0.0 8 1.1237583874516644E-4 0.0 0.0 0.0 0.0 9 1.1237583874516644E-4 0.0 0.0 0.0 0.0 10-11 1.1237583874516644E-4 0.0 0.0 0.0 0.0 12-13 1.1237583874516644E-4 0.0 0.0 0.0 5.618791937258322E-5 14-15 1.1237583874516644E-4 0.0 0.0 0.0 1.1237583874516643E-4 16-17 1.1237583874516644E-4 0.0 0.0 0.0 1.6856375811774964E-4 18-19 1.1237583874516644E-4 0.0 0.0 5.618791937258322E-5 1.6856375811774964E-4 20-21 1.1237583874516644E-4 0.0 0.0 5.618791937258322E-5 1.6856375811774964E-4 22-23 1.1237583874516644E-4 0.0 0.0 1.6856375811774964E-4 1.9665771780404126E-4 24-25 2.2475167749033285E-4 0.0 0.0 4.2140939529437414E-4 2.809395968629161E-4 26-27 2.809395968629161E-4 0.0 0.0 9.551946293339147E-4 2.809395968629161E-4 28-29 4.4950335498066576E-4 0.0 0.0 0.0032588993236098264 2.809395968629161E-4 30-31 4.4950335498066576E-4 0.0 0.0 0.009355288575535105 3.0903355654920766E-4 32-33 4.4950335498066576E-4 0.0 0.0 0.02286848318464137 3.371275162354993E-4 34-35 4.4950335498066576E-4 0.0 0.0 0.0431804160378302 3.371275162354993E-4 36-37 4.4950335498066576E-4 0.0 0.0 0.08015206698498996 3.371275162354993E-4 38-39 4.4950335498066576E-4 0.0 0.0 0.1378851541403192 3.371275162354993E-4 40-41 4.4950335498066576E-4 0.0 0.0 0.19932664397423897 3.371275162354993E-4 42-43 4.775973146669574E-4 0.0 0.0 0.2632404022605524 3.371275162354993E-4 44-45 5.618791937258322E-4 0.0 0.0 0.3441510061570722 3.371275162354993E-4 46-47 6.46161072784707E-4 0.0 0.0 0.43879955634018863 3.371275162354993E-4 48-49 6.742550324709986E-4 0.0 0.0 0.5435057440909974 3.933154356080825E-4 50-51 6.742550324709986E-4 0.0 0.0 0.6551792338440066 4.4950335498066576E-4 52-53 6.742550324709986E-4 0.0 0.0 0.7645209249430536 4.775973146669574E-4 54-55 6.742550324709986E-4 0.0 0.0 0.8854654213925389 5.05691274353249E-4 56-57 6.742550324709986E-4 0.0 0.0 1.0326215822293343 5.05691274353249E-4 58-59 6.742550324709986E-4 0.0 0.0 1.1920828974087256 5.05691274353249E-4 60-61 6.742550324709986E-4 0.0 0.0 1.362472762906084 5.05691274353249E-4 62-63 6.742550324709986E-4 0.0 0.0 1.5310365210238337 5.05691274353249E-4 64-65 6.742550324709986E-4 0.0 0.0 1.7117930576454339 5.618791937258322E-4 66-67 7.304429518435818E-4 0.0 0.0 1.9205030841548945 5.618791937258322E-4 68-69 7.304429518435818E-4 0.0 0.0 2.1578970435040583 5.618791937258322E-4 70-71 7.86630871216165E-4 0.0 0.0 2.412315942423115 5.618791937258322E-4 72-73 7.86630871216165E-4 0.0 0.0 2.6665100896646816 6.46161072784707E-4 74-75 7.86630871216165E-4 0.0 0.0 2.927924384545625 7.304429518435818E-4 76-77 7.86630871216165E-4 0.0 0.0 3.211532907578739 7.304429518435818E-4 78-79 7.86630871216165E-4 0.0 0.0 3.534950571487328 7.304429518435818E-4 80-81 8.428187905887483E-4 0.0 0.0 3.8830066380407944 7.86630871216165E-4 82-83 8.428187905887483E-4 0.0 0.0 4.235642020023127 8.990067099613315E-4 84-85 8.428187905887483E-4 0.0 0.0 4.598222663734406 8.990067099613315E-4 86-87 8.428187905887483E-4 0.0 0.0 4.9688381799159655 8.990067099613315E-4 88-89 8.428187905887483E-4 0.0 0.0 5.375694904092841 8.990067099613315E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3060 0.0 57.037106 1 GTATCAA 5295 0.0 40.596195 1 ATCAACG 7015 0.0 30.630394 3 TCAACGC 7125 0.0 30.090784 4 TATCAAC 7130 0.0 30.081526 2 CAACGCA 7290 0.0 29.40972 5 AACGCAG 7500 0.0 28.49722 6 TAACGCA 135 1.3349467E-5 24.649384 4 TGGTATC 1505 0.0 24.64739 2 ACGCAGA 8720 0.0 24.455755 7 CGCAGAG 8810 0.0 24.044191 8 GTGGTAT 1690 0.0 22.512106 1 GCAGAGT 9870 0.0 21.317575 9 GAGTACT 5915 0.0 21.158121 12-13 GTAAGGT 790 0.0 21.061186 4 TACATGG 5850 0.0 20.97378 2 TAAGGTG 800 0.0 20.79792 5 GTACATG 6020 0.0 20.776487 1 AGAGTAC 8945 0.0 20.229998 10-11 CAGAGTA 9345 0.0 20.126448 10-11 >>END_MODULE