FastQCFastQC Report
Thu 26 May 2016
SRR938505_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938505_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1781202
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60080.3373003174260977No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47870.26875110178407613No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33140.18605413647637944No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29550.057.0958181
GTATCAA52950.041.404771
ATCAACG66600.032.3970833
TATCAAC67150.032.2732772
TCAACGC67200.032.0370984
CAACGCA68450.031.5909145
AACGCAG70700.030.6501856
GTACATG58150.027.1291871
CGCAGAG80600.026.7585188
TACATGG57750.026.6635072
ACGCAGA81450.026.6542457
TGGTATC13050.026.2208162
GTGGTAT14400.024.8231741
ACATGGG60700.024.6630123
GCAGAGT90500.023.0958089
GAGTACT54950.022.56199312-13
GTACTTT58500.021.19612114-15
CAGAGTA87250.021.17878210-11
GTACTAG1856.366874E-620.6099411
AGTACTT57150.020.48827412-13
Produced by FastQC (version 0.11.5)