##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938505_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1781202 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.963000827531072 31.0 31.0 34.0 28.0 34.0 2 31.098646307381195 31.0 31.0 34.0 27.0 34.0 3 31.151822757890457 31.0 31.0 34.0 27.0 34.0 4 34.81604781490252 37.0 35.0 37.0 32.0 37.0 5 34.81710328194107 37.0 35.0 37.0 32.0 37.0 6 34.81660867212141 37.0 35.0 37.0 32.0 37.0 7 34.72127473470162 37.0 35.0 37.0 31.0 37.0 8 34.681670579754574 37.0 35.0 37.0 30.0 37.0 9 36.14294223788206 39.0 35.0 39.0 31.0 39.0 10-11 35.99116691986647 39.0 35.0 39.0 30.0 39.0 12-13 35.808607333699385 38.0 35.0 39.0 30.0 39.0 14-15 36.76296006853799 39.0 36.0 41.0 30.0 41.0 16-17 36.568670762777046 39.0 36.0 40.5 30.0 41.0 18-19 36.422823183445786 39.0 36.0 40.0 29.5 41.0 20-21 36.40263541136828 39.0 35.5 40.5 29.5 41.0 22-23 36.487966552923254 39.0 36.0 41.0 30.0 41.0 24-25 36.38483479133754 39.0 35.5 41.0 29.0 41.0 26-27 36.17239847024649 39.0 35.0 40.5 28.0 41.0 28-29 35.9764970508679 39.0 35.0 40.0 27.0 41.0 30-31 35.69587503270263 38.0 34.5 40.0 27.0 41.0 32-33 35.378705784071656 38.0 34.0 40.0 26.5 41.0 34-35 35.095679209881865 38.0 34.0 40.0 25.0 41.0 36-37 34.78369578520572 38.0 33.5 40.0 24.5 41.0 38-39 34.44489086583105 38.0 33.0 40.0 24.0 41.0 40-41 34.30823230604951 38.0 33.0 40.0 24.0 41.0 42-43 34.0609863451759 38.0 33.0 40.0 23.0 41.0 44-45 33.72527736887787 38.0 33.0 40.0 21.5 41.0 46-47 33.313417568585706 37.5 32.0 40.0 18.5 41.0 48-49 32.89926437315925 37.0 31.0 40.0 16.0 41.0 50-51 32.101120479316776 36.0 30.5 39.0 14.0 40.5 52-53 32.113059046643784 36.5 31.0 39.0 13.0 40.0 54-55 32.14903756002968 36.5 31.0 39.0 9.0 41.0 56-57 31.78266137136608 36.0 30.0 39.0 7.5 41.0 58-59 31.304115984599164 36.0 29.5 39.0 4.5 41.0 60-61 30.789769773445123 35.0 28.5 39.0 2.0 40.0 62-63 30.167888032912607 35.0 28.0 38.5 2.0 40.0 64-65 29.625731949548673 34.5 27.5 38.0 2.0 40.0 66-67 29.039313901511452 34.0 26.0 38.0 2.0 40.0 68-69 28.38601208622043 34.0 26.0 37.0 2.0 39.5 70-71 27.71376856751789 33.5 25.0 36.5 2.0 39.0 72-73 27.050159106041875 33.0 23.0 36.0 2.0 39.0 74-75 26.32601804848636 32.0 21.0 35.0 2.0 38.0 76-77 25.56606886810143 31.5 19.0 35.0 2.0 37.0 78-79 24.743096796432972 31.0 13.5 35.0 2.0 37.0 80-81 23.957957042491532 31.0 7.0 34.5 2.0 36.0 82-83 23.204120026813357 30.0 2.0 34.0 2.0 36.0 84-85 22.45222804600489 29.5 2.0 34.0 2.0 35.0 86-87 21.700210868840255 29.0 2.0 34.0 2.0 35.0 88-89 20.908022223195346 28.5 2.0 34.0 2.0 35.0 90-91 20.07816519406558 26.5 2.0 34.0 2.0 35.0 92-93 19.200661968715508 25.5 2.0 33.0 2.0 35.0 94-95 18.30518632923161 24.5 2.0 33.0 2.0 35.0 96-97 17.23431677036069 20.0 2.0 32.5 2.0 35.0 98-99 16.012624620902066 4.5 2.0 32.0 2.0 34.0 100-101 13.38425933723407 2.0 2.0 28.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5714329118501844 1101 2 0.6759523968304499 1101 3 0.6484983210299653 1101 4 0.544301204436394 1101 5 0.6726792671156261 1101 6 0.4761249282237827 1101 7 0.4882910247614447 1101 8 0.5109695126704352 1101 9 0.5422025350623514 1101 10-11 0.5709072508767647 1101 12-13 0.8226601614053592 1101 14-15 0.9329252226866274 1101 16-17 1.0320101873472893 1101 18-19 1.0391726133258175 1101 20-21 1.055827461723723 1101 22-23 0.900280849085803 1101 24-25 1.0789754616908027 1101 26-27 1.0547476765367847 1101 28-29 1.1436378640927316 1101 30-31 1.2777225555422334 1101 32-33 1.4271365347174054 1101 34-35 1.3698591041779267 1101 36-37 1.4762172537691143 1101 38-39 1.679913558202685 1101 40-41 1.8163811576335576 1101 42-43 1.8900054755047933 1101 44-45 2.0030802995684027 1101 46-47 2.112114273426407 1101 48-49 2.280992244215966 1101 50-51 2.269613525923482 1101 52-53 2.5013854429804496 1101 54-55 2.648496875683385 1101 56-57 2.617710890622348 1101 58-59 2.831353709417211 1101 60-61 2.8416230186467253 1101 62-63 2.696499628063357 1101 64-65 2.9881313509928873 1101 66-67 3.0608849824480444 1101 68-69 3.249238635197628 1101 70-71 3.4777833393147617 1101 72-73 3.5473719363688225 1101 74-75 3.6790173846228775 1101 76-77 3.7837191975772377 1101 78-79 4.1296017493829815 1101 80-81 4.436751769455828 1101 82-83 4.612115486139025 1101 84-85 4.79833218328244 1101 86-87 4.918402164603169 1101 88-89 4.914367294606819 1101 90-91 5.354489112644618 1101 92-93 5.632847253108043 1101 94-95 5.816559221509859 1101 96-97 6.064100704088979 1101 98-99 6.28981617042011 1101 100-101 6.016170176853771 1102 1 0.503286670503261 1102 2 0.4165794474876563 1102 3 0.45489061321005053 1102 4 0.45124902946904655 1102 5 0.4685958355308699 1102 6 0.5644049716289032 1102 7 0.5212771599124011 1102 8 0.5773190279089988 1102 9 0.6798651902790525 1102 10-11 0.7112050480973195 1102 12-13 0.673762057728176 1102 14-15 0.8503994310548464 1102 16-17 1.0009156805671822 1102 18-19 0.8376655586428612 1102 20-21 1.0001458388882796 1102 22-23 0.7042110861286872 1102 24-25 0.6220217432614277 1102 26-27 0.681993373090684 1102 28-29 0.6084296313045741 1102 30-31 0.7960106605737067 1102 32-33 0.64011308507758 1102 34-35 0.9313262188553182 1102 36-37 1.188216412144783 1102 38-39 1.256977572649447 1102 40-41 1.4182273274829384 1102 42-43 1.3802754606210073 1102 44-45 1.4004554921169898 1102 46-47 1.4437946412554226 1102 48-49 1.6559515899104582 1102 50-51 1.7650877687402087 1102 52-53 1.7249104980220764 1102 54-55 2.0033960445624714 1102 56-57 2.1086642733476353 1102 58-59 1.968782317417169 1102 60-61 1.961472755605353 1102 62-63 2.3887065987527407 1102 64-65 2.302743496253459 1102 66-67 2.428301962387863 1102 68-69 2.8082881062948175 1102 70-71 2.6247734346932248 1102 72-73 2.788249245305405 1102 74-75 2.8140038635834514 1102 76-77 3.1049187756843324 1102 78-79 3.545332593057342 1102 80-81 4.032851455332715 1102 82-83 4.355613752399648 1102 84-85 4.303294794830595 1102 86-87 4.467960227394695 1102 88-89 4.736693372701588 1102 90-91 4.8575545232691475 1102 92-93 4.543444593615671 1102 94-95 4.886992911340219 1102 96-97 4.681067573742919 1102 98-99 4.963978033152262 1102 100-101 4.934753829820851 1103 1 0.6338220307147644 1103 2 0.6380458326672347 1103 3 0.46972836242558813 1103 4 0.4656199507403542 1103 5 0.40130138420610706 1103 6 0.6127304870647023 1103 7 0.6168540052463669 1103 8 0.5159961241429301 1103 9 0.5589086261327196 1103 10-11 0.6143283035083513 1103 12-13 0.7183284050481689 1103 14-15 0.8716449151762831 1103 16-17 1.1406737000616616 1103 18-19 1.1599295618769645 1103 20-21 1.1904962967325972 1103 22-23 0.9816823866375515 1103 24-25 1.212831168373235 1103 26-27 1.3367405801441166 1103 28-29 1.450320300520886 1103 30-31 1.4381158139691976 1103 32-33 1.483700697934431 1103 34-35 1.4727567978503018 1103 36-37 1.57289081970643 1103 38-39 1.6880744097698042 1103 40-41 1.532318472831669 1103 42-43 1.7843283613711378 1103 44-45 1.7517792942461128 1103 46-47 2.0354732326221523 1103 48-49 1.8263648048309875 1103 50-51 1.9121623136230639 1103 52-53 2.010640321750408 1103 54-55 2.4182780702428204 1103 56-57 2.206977596713415 1103 58-59 2.547971095579239 1103 60-61 2.7477584414734615 1103 62-63 2.8445037676257527 1103 64-65 2.938944479319318 1103 66-67 2.9089926110701576 1103 68-69 3.189540231885978 1103 70-71 3.3186615179073087 1103 72-73 3.4451395295089746 1103 74-75 3.664159312476219 1103 76-77 3.7359516044370835 1103 78-79 3.835515409938864 1103 80-81 4.030305879449916 1103 82-83 4.387588578983493 1103 84-85 4.764786943779189 1103 86-87 4.637237173473661 1103 88-89 4.948444763560104 1103 90-91 5.3439480127037555 1103 92-93 5.416289003551565 1103 94-95 5.368022852497436 1103 96-97 5.488258007459766 1103 98-99 5.4697245086814945 1103 100-101 5.185019969875652 1104 1 0.5578625489947022 1104 2 0.4426901913326873 1104 3 0.4479424110573582 1104 4 0.45469501282084934 1104 5 0.4572893860746632 1104 6 0.42755850565197306 1104 7 0.40133756928147335 1104 8 0.37381949591290464 1104 9 0.4819042251787735 1104 10-11 0.63328067977762 1104 12-13 0.5953696855044939 1104 14-15 0.725878682048652 1104 16-17 0.8523405306372283 1104 18-19 0.8770152206678148 1104 20-21 0.93723430474774 1104 22-23 0.8510687117180709 1104 24-25 0.9961321296989354 1104 26-27 0.7807286065990198 1104 28-29 0.9374972975459315 1104 30-31 1.0887827183037686 1104 32-33 1.11352699547826 1104 34-35 0.957583955915851 1104 36-37 1.3115276303670669 1104 38-39 1.2794089560289876 1104 40-41 1.5172206192187048 1104 42-43 1.3753801647788109 1104 44-45 1.4145832428114957 1104 46-47 1.5464123076842284 1104 48-49 1.5588949845570923 1104 50-51 1.739415036559656 1104 52-53 1.8287856515760907 1104 54-55 2.0593028286820783 1104 56-57 2.1064539077797235 1104 58-59 2.3426715110883016 1104 60-61 2.5201824082327384 1104 62-63 2.8370307511283173 1104 64-65 2.768632478729245 1104 66-67 2.8613824028238994 1104 68-69 3.0379986686868037 1104 70-71 3.0450518751467577 1104 72-73 3.2788529266028696 1104 74-75 3.379806056016161 1104 76-77 3.6488778700186693 1104 78-79 4.05590485748392 1104 80-81 4.379718266045444 1104 82-83 4.303162792835437 1104 84-85 4.251855760930614 1104 86-87 4.332870025734369 1104 88-89 4.888312734941607 1104 90-91 5.061727006139204 1104 92-93 5.271846744559113 1104 94-95 5.312056363671925 1104 96-97 5.633710764817231 1104 98-99 5.626415065070251 1104 100-101 5.123776638918999 1105 1 0.5125696107324949 1105 2 0.617803623351584 1105 3 0.5880472512541672 1105 4 0.5018901323370315 1105 5 0.4041498767305427 1105 6 0.3768538352948738 1105 7 0.4818005997809749 1105 8 0.39716217865208847 1105 9 0.5789545228680808 1105 10-11 0.5965985031983507 1105 12-13 0.6921185646916044 1105 14-15 0.8072635862818416 1105 16-17 0.8821909460162871 1105 18-19 0.898608266636117 1105 20-21 0.8699288598933776 1105 22-23 0.9260571717793127 1105 24-25 0.7624101970029145 1105 26-27 0.9260917534962658 1105 28-29 1.0454115813473805 1105 30-31 1.068005836706476 1105 32-33 1.1389863613865217 1105 34-35 1.243236934394595 1105 36-37 1.378115719740805 1105 38-39 1.5149960717020505 1105 40-41 1.6032590845066892 1105 42-43 1.5156056898313395 1105 44-45 1.4943513810699542 1105 46-47 1.6081854824065402 1105 48-49 1.6339825471458482 1105 50-51 1.4997349130911246 1105 52-53 1.7060328564443594 1105 54-55 1.9951572128604411 1105 56-57 1.990079938592828 1105 58-59 1.836396192227756 1105 60-61 2.0636608159208514 1105 62-63 2.3975674199444086 1105 64-65 2.6953810290427853 1105 66-67 2.9000843623231205 1105 68-69 2.7435135261377255 1105 70-71 2.865163022668874 1105 72-73 3.2307992121520464 1105 74-75 3.3103481042642606 1105 76-77 3.5107751945147037 1105 78-79 3.7024456360451197 1105 80-81 4.121011338206177 1105 82-83 4.4048946939243585 1105 84-85 4.567312319668023 1105 86-87 4.804191222458321 1105 88-89 5.132416942056789 1105 90-91 5.059581399713952 1105 92-93 5.303328754587408 1105 94-95 5.493358785238517 1105 96-97 5.682307414300251 1105 98-99 5.827807551013844 1105 100-101 5.234077458401467 1106 1 0.354957571421771 1106 2 0.29473576759077247 1106 3 0.3173722958808618 1106 4 0.2917407465893973 1106 5 0.328638475300707 1106 6 0.028510595815809836 1106 7 0.28371602572740784 1106 8 0.2536371957270447 1106 9 0.32316821029039744 1106 10-11 0.16174857551053634 1106 12-13 0.13061174273652654 1106 14-15 0.5488553995790895 1106 16-17 0.33990659415998437 1106 18-19 0.4799813244163147 1106 20-21 0.5233216479339617 1106 22-23 0.3923758304639975 1106 24-25 0.5438196337881465 1106 26-27 0.6709144325959926 1106 28-29 0.48294936468900573 1106 30-31 0.6707881172777732 1106 32-33 0.9253925615102858 1106 34-35 0.790723127313889 1106 36-37 0.7994380965738017 1106 38-39 0.8822760099290718 1106 40-41 1.2572795887125636 1106 42-43 0.732297054989175 1106 44-45 1.061911562465653 1106 46-47 1.4183971939495663 1106 48-49 1.4158315635752459 1106 50-51 0.8942090492234982 1106 52-53 1.434087756642839 1106 54-55 1.378256668423667 1106 56-57 1.3338499448164924 1106 58-59 0.8730970801251452 1106 60-61 2.1070672995868556 1106 62-63 2.2991782608879063 1106 64-65 2.2718326672571507 1106 66-67 2.3769141460285397 1106 68-69 1.890716087647668 1106 70-71 2.2345539239943832 1106 72-73 2.7700256768082383 1106 74-75 3.094452529841554 1106 76-77 3.282642721437842 1106 78-79 3.6150312559950812 1106 80-81 3.9848655867924805 1106 82-83 4.27640146238091 1106 84-85 4.198750199841999 1106 86-87 4.495153357977248 1106 88-89 4.490158091169693 1106 90-91 5.002835055893126 1106 92-93 5.2511329071438375 1106 94-95 5.104053499404753 1106 96-97 5.3778053340988095 1106 98-99 5.246027904353845 1106 100-101 4.76995937286444 1107 1 0.315627834576901 1107 2 0.38498347252468434 1107 3 0.43742114370925833 1107 4 0.3750580345897063 1107 5 0.367195702293742 1107 6 0.3022025510942541 1107 7 0.3991274805781728 1107 8 0.3822361639246097 1107 9 0.3520883185351309 1107 10-11 0.4872439095276988 1107 12-13 0.5118535231500161 1107 14-15 0.7030863496845186 1107 16-17 0.8651027264762163 1107 18-19 0.9112384500028483 1107 20-21 0.8293417401917011 1107 22-23 0.821778407286132 1107 24-25 0.7167075136687018 1107 26-27 0.7863255957134356 1107 28-29 0.964150944249937 1107 30-31 1.0385115587508125 1107 32-33 1.1469103449904736 1107 34-35 1.1669322160906788 1107 36-37 1.1919845434369023 1107 38-39 1.3886279196737803 1107 40-41 1.4448177328451663 1107 42-43 1.473631116488889 1107 44-45 1.6494178493188656 1107 46-47 1.561252586406738 1107 48-49 1.7863851100606212 1107 50-51 1.752034562353252 1107 52-53 1.8043510284671243 1107 54-55 2.0192572203831176 1107 56-57 2.0228873065203032 1107 58-59 2.1807211222187597 1107 60-61 2.1479487660019423 1107 62-63 2.399496308561801 1107 64-65 2.466506608586073 1107 66-67 2.698686671050506 1107 68-69 2.8065699852632733 1107 70-71 2.9026567938334793 1107 72-73 3.1642332532394555 1107 74-75 3.247930224494784 1107 76-77 3.469177886389698 1107 78-79 3.5274596208764564 1107 80-81 3.8091481853662543 1107 82-83 3.899344596553732 1107 84-85 4.213863736618489 1107 86-87 4.472302469882653 1107 88-89 4.659931426043379 1107 90-91 4.908147169711132 1107 92-93 5.0446612700414235 1107 94-95 5.073664805588212 1107 96-97 5.057088591989903 1107 98-99 4.964080187633911 1107 100-101 4.825031040073734 1108 1 0.3246137906079518 1108 2 0.40631386171123296 1108 3 0.35190690620191845 1108 4 0.2675601800928433 1108 5 0.16111104968801016 1108 6 0.3078477543438751 1108 7 0.24249002858398683 1108 8 0.2975046562748389 1108 9 0.4270568578262868 1108 10-11 0.4715089810797224 1108 12-13 0.3713350746818307 1108 14-15 0.5454554768521049 1108 16-17 0.7681481402452874 1108 18-19 0.8238940311411795 1108 20-21 0.7318106839981056 1108 22-23 0.7502323814554757 1108 24-25 0.754385607581419 1108 26-27 0.6410444447517776 1108 28-29 0.7179122213631786 1108 30-31 0.8513110501193424 1108 32-33 0.9815056324878952 1108 34-35 0.9647550664280402 1108 36-37 1.2350343997267927 1108 38-39 1.223653308365769 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0.7928055937779988 2311 44-45 0.9916551073744344 2311 46-47 0.8982319552714699 2311 48-49 0.9377663423827372 2311 50-51 1.0606070208968674 2311 52-53 1.031521457173234 2311 54-55 1.0669534102782947 2311 56-57 0.9767706185939602 2311 58-59 1.1528557791984007 2311 60-61 1.3122202983628704 2311 62-63 1.4967923542608723 2311 64-65 1.576141404215825 2311 66-67 1.6931617417620544 2311 68-69 1.407937629875331 2311 70-71 1.6383214824253471 2311 72-73 1.8174133319927144 2311 74-75 2.021004374569653 2311 76-77 2.1754637274412225 2311 78-79 2.301925817981445 2311 80-81 1.9778398829981079 2311 82-83 2.4147855038800046 2311 84-85 2.8799998355592002 2311 86-87 3.0708119812796895 2311 88-89 2.914074568409305 2311 90-91 3.0584216969142837 2311 92-93 3.1207094648170894 2311 94-95 3.0798560872697642 2311 96-97 3.323373323840606 2311 98-99 3.151738110100773 2311 100-101 3.095082063311489 2312 1 -0.2535391367427131 2312 2 -0.30168276404370786 2312 3 -0.19871571356262763 2312 4 -0.14840382644553785 2312 5 -0.1881005995779006 2312 6 -0.01936401019833056 2312 7 -0.16680300120358282 2312 8 -0.18012450215425702 2312 9 -0.07187030601057387 2312 10-11 -0.23045750191148073 2312 12-13 -0.19982830448093125 2312 14-15 -0.2201411077895159 2312 16-17 -0.15452588366120068 2312 18-19 -0.058023859768269404 2312 20-21 -0.01812748986069579 2312 22-23 -0.1475125757034803 2312 24-25 -0.039790554473206896 2312 26-27 0.021497957598121786 2312 28-29 -0.024449659321305717 2312 30-31 0.0542352349441515 2312 32-33 0.20066546136789754 2312 34-35 0.07043787546717795 2312 36-37 0.26172497912646264 2312 38-39 0.3212584007387065 2312 40-41 0.25710313172547217 2312 42-43 0.4781077227267474 2312 44-45 0.41688521561206215 2312 46-47 0.645865462115502 2312 48-49 0.7843224304049841 2312 50-51 0.8029868716225863 2312 52-53 0.7966612296814333 2312 54-55 0.869099159266149 2312 56-57 1.0819971464520677 2312 58-59 1.099278429230079 2312 60-61 1.1643032530022204 2312 62-63 1.2030578807143684 2312 64-65 1.2612422056700083 2312 66-67 1.3335726591654549 2312 68-69 1.5542886723165985 2312 70-71 1.6629701921116506 2312 72-73 1.780938628751091 2312 74-75 1.7429410844762891 2312 76-77 1.9820664981041425 2312 78-79 2.136123950675813 2312 80-81 2.238160464672074 2312 82-83 2.417630941650252 2312 84-85 2.634471178752346 2312 86-87 2.8353806832544066 2312 88-89 2.813603748502395 2312 90-91 2.9717999226985192 2312 92-93 3.4683636276968137 2312 94-95 3.3199820890874925 2312 96-97 3.3607863619303373 2312 98-99 3.603444574062877 2312 100-101 3.173476951631189 2313 1 -0.08753461563831166 2313 2 -0.23695297708329122 2313 3 -0.28456486743539244 2313 4 -0.27600307344551567 2313 5 -0.14727983841815728 2313 6 -0.1156129237141954 2313 7 -0.13575145085723506 2313 8 -0.24354967665691163 2313 9 -0.2804553045471465 2313 10-11 -0.21474949119742348 2313 12-13 -0.2704052885340005 2313 14-15 -0.29164243485228525 2313 16-17 -0.17661407845671562 2313 18-19 -0.010489763013524112 2313 20-21 0.0575293618630468 2313 22-23 -0.18937907784540897 2313 24-25 -0.020634481026874596 2313 26-27 -0.1317457795968835 2313 28-29 0.08856658694251962 2313 30-31 0.2115893190158502 2313 32-33 0.12345205481884136 2313 34-35 0.1720253895471231 2313 36-37 0.1868411386932891 2313 38-39 0.4449117978863484 2313 40-41 0.40667097711656197 2313 42-43 0.5143315750447286 2313 44-45 0.4085524088909409 2313 46-47 0.5070661434962673 2313 48-49 0.6864795448804983 2313 50-51 0.832310856757708 2313 52-53 0.9152519433462984 2313 54-55 0.6481694004656333 2313 56-57 1.034067674793775 2313 58-59 0.9287194054249817 2313 60-61 1.0305867122790175 2313 62-63 1.0863011633598916 2313 64-65 1.2946154229491 2313 66-67 1.3130864741438586 2313 68-69 1.5499531294293014 2313 70-71 1.8429033289909142 2313 72-73 1.810837729851766 2313 74-75 1.8818261854527947 2313 76-77 1.8477713768613846 2313 78-79 2.175007887562174 2313 80-81 2.310434664443246 2313 82-83 2.524774353121753 2313 84-85 2.546865357414685 2313 86-87 2.7478107546962853 2313 88-89 2.7585226058758536 2313 90-91 2.969795470330876 2313 92-93 3.0017810526850646 2313 94-95 3.174132403059236 2313 96-97 3.5275838501563967 2313 98-99 3.485928565051708 2313 100-101 3.022867522722729 2314 1 -0.31913402552384085 2314 2 -0.28660855876755775 2314 3 -0.43643670610785534 2314 4 -0.22502361652830416 2314 5 -0.1940630489958508 2314 6 -0.2911991558554661 2314 7 -0.3084004214846203 2314 8 -0.2903395426609947 2314 9 -0.1776023451008797 2314 10-11 -0.23137585555982554 2314 12-13 -0.31574565813201616 2314 14-15 -0.3544227946890075 2314 16-17 -0.38180115140018955 2314 18-19 -0.3227576550181439 2314 20-21 -0.14933987010074645 2314 22-23 -0.2572904941201415 2314 24-25 -0.19249561555522376 2314 26-27 0.0022326679139865746 2314 28-29 0.020812682425699336 2314 30-31 0.020069164166265807 2314 32-33 0.15986694300767823 2314 34-35 0.14251384238233555 2314 36-37 0.2682949611265144 2314 38-39 0.3687762157583876 2314 40-41 0.4642027013156991 2314 42-43 0.5399081013558487 2314 44-45 0.48711666703274403 2314 46-47 0.48065824350513964 2314 48-49 0.5362521511454759 2314 50-51 0.6728335295769412 2314 52-53 0.7186143202209081 2314 54-55 0.6544596103334115 2314 56-57 0.7772170225619917 2314 58-59 0.8657133157098365 2314 60-61 0.8915066031300256 2314 62-63 1.0281219216348063 2314 64-65 1.0965258076327444 2314 66-67 1.3149253129938003 2314 68-69 1.5042616860891656 2314 70-71 1.5506031027064182 2314 72-73 1.4575593777353397 2314 74-75 1.7005717485018685 2314 76-77 2.0242347387391 2314 78-79 2.3965248810842255 2314 80-81 2.447412768090423 2314 82-83 2.562447394207922 2314 84-85 2.650487573227977 2314 86-87 3.0453435326712572 2314 88-89 3.0529847364996883 2314 90-91 3.0624035100859537 2314 92-93 3.204320652472088 2314 94-95 3.25485884284981 2314 96-97 3.4304634313082296 2314 98-99 3.2778499866507858 2314 100-101 2.979000498142023 2315 1 -0.4583068557961454 2315 2 -0.4521450002202272 2315 3 -0.6405088410700408 2315 4 -0.5527529165089149 2315 5 -0.5067005181778228 2315 6 -0.4126485034828278 2315 7 -0.6681112167975058 2315 8 -0.6143706792575756 2315 9 -0.7056862255407808 2315 10-11 -0.6626454198136926 2315 12-13 -0.5992701324941905 2315 14-15 -0.8019074895558944 2315 16-17 -0.6905105339315654 2315 18-19 -0.616210382240503 2315 20-21 -0.6048621837382413 2315 22-23 -0.6703656485565546 2315 24-25 -0.5715365862192314 2315 26-27 -0.6124865763864094 2315 28-29 -0.6450991156850492 2315 30-31 -0.6570880978077511 2315 32-33 -0.43342372986415256 2315 34-35 -0.5938652816931196 2315 36-37 -0.3631311171582041 2315 38-39 -0.33591643500756163 2315 40-41 -0.10968854220905655 2315 42-43 -0.07781122604934865 2315 44-45 -0.05005472275644962 2315 46-47 -0.21787080162075512 2315 48-49 -0.26837540086427936 2315 50-51 0.01650509942652434 2315 52-53 0.3726075056277054 2315 54-55 0.3991561393231322 2315 56-57 0.6906052050601232 2315 58-59 0.7041478983852585 2315 60-61 0.7074918104764869 2315 62-63 0.8180413099835135 2315 64-65 1.0798799171648668 2315 66-67 1.2338662808876464 2315 68-69 1.4709117317968037 2315 70-71 1.423528945527547 2315 72-73 1.4781652116919624 2315 74-75 1.673928351177839 2315 76-77 1.7806739752830474 2315 78-79 2.031193431459581 2315 80-81 2.1165254858942397 2315 82-83 2.4930742813793074 2315 84-85 2.555856142994454 2315 86-87 2.6653330060638716 2315 88-89 2.931366409122198 2315 90-91 2.9914347851905276 2315 92-93 3.2981182645943115 2315 94-95 3.3614670591438944 2315 96-97 3.5371076596802062 2315 98-99 3.5036567957036695 2315 100-101 3.2298523222922757 2316 1 -1.077485204189852 2316 2 -1.1067704341444937 2316 3 -1.025854788018659 2316 4 -0.8635919692511393 2316 5 -0.9984866520340745 2316 6 -0.7791773743522654 2316 7 -0.8659806817788294 2316 8 -1.032224068062554 2316 9 -0.9283501009592499 2316 10-11 -0.9009985833755323 2316 12-13 -0.9484897312964264 2316 14-15 -1.7009393857497273 2316 16-17 -1.408235843309626 2316 18-19 -1.1824738195389983 2316 20-21 -0.893577502921012 2316 22-23 -1.0259454950379023 2316 24-25 -1.2164739156306936 2316 26-27 -1.1233680278354754 2316 28-29 -1.1060176660026002 2316 30-31 -0.9857315158501692 2316 32-33 -0.7909702580283735 2316 34-35 -1.0409583203554433 2316 36-37 -0.9338521601663956 2316 38-39 -1.1695434677163945 2316 40-41 -0.9360742291122719 2316 42-43 -0.638244841157416 2316 44-45 -0.6066561130807102 2316 46-47 -0.2833052558699052 2316 48-49 -0.206118641362103 2316 50-51 -0.09967795699025572 2316 52-53 0.027081894158854425 2316 54-55 0.05573500533439102 2316 56-57 0.14247339808023796 2316 58-59 0.3124293374680356 2316 60-61 0.3046802462292 2316 62-63 0.2856645974732466 2316 64-65 0.6792452615218636 2316 66-67 0.9953572137475319 2316 68-69 1.0039959727274521 2316 70-71 0.8489412287862379 2316 72-73 1.1723698384508445 2316 74-75 1.2649226008532253 2316 76-77 1.4746723499992882 2316 78-79 1.4704025189157512 2316 80-81 1.6536287709107143 2316 82-83 1.9397980979695717 2316 84-85 1.9096018037518014 2316 86-87 2.2943739119829125 2316 88-89 2.1281926892539857 2316 90-91 2.3438228040226186 2316 92-93 2.32160935644934 2316 94-95 2.1469516851961323 2316 96-97 2.2269438946750384 2316 98-99 2.50190564078056 2316 100-101 2.3038036167777936 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6079.0 3 2386.0 4 2711.0 5 3207.0 6 3740.0 7 4652.0 8 5991.0 9 7564.0 10 9446.0 11 11684.0 12 14036.0 13 16198.0 14 18465.0 15 21116.0 16 24006.0 17 27073.0 18 26849.0 19 26411.0 20 28162.0 21 30799.0 22 33890.0 23 37321.0 24 41407.0 25 45706.0 26 50428.0 27 55987.0 28 61937.0 29 68414.0 30 76180.0 31 85277.0 32 97699.0 33 111621.0 34 129820.0 35 152611.0 36 173202.0 37 171359.0 38 91191.0 39 6577.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.94529545286894 18.18863687427043 14.147906346590144 23.718161326270494 2 15.70641814364807 19.546532355805464 39.94409579982769 24.80295370071877 3 19.493805401191402 23.391083686901197 29.153256924481948 27.961853987425457 4 13.034073777839689 15.92442478313521 35.84054343698958 35.200958002035534 5 14.257297260858817 36.777576792575786 33.85114486663821 15.113981079927196 6 33.44929786500178 35.05158577237097 16.935165846320125 14.563950516307125 7 29.703148779405737 31.94716654779889 19.941595673587205 18.40808899920817 8 26.99358767719812 34.857303634548146 19.15536825647494 18.993740431778793 9 26.686354042520687 16.48555628818454 19.4910637361008 37.33702593319397 10-11 25.5878197926404 26.128163317798535 26.830191508438183 21.45382538112288 12-13 26.331331370683586 23.295729608117778 27.367844069312014 23.005094951886626 14-15 22.906259950435253 24.870542555993396 25.461208816078614 26.76198867749274 16-17 22.83919195175003 28.15082129784236 26.183626041976975 22.82636070843063 18-19 22.913114174580308 26.453481107644688 28.428201075568015 22.20520364220699 20-21 23.22172201364857 25.758285899949986 28.67396114361328 22.346030942788165 22-23 23.02502871901807 25.57914697426085 28.651766005499784 22.744058301221294 24-25 22.84629436354996 25.82284126693457 28.68833535366699 22.642529015848478 26-27 22.414501500392475 26.226467288354794 28.472604672911633 22.8864265383411 28-29 22.5503556989478 26.504619326445226 28.528170463662487 22.416854510944486 30-31 22.586615729892817 26.05812498455497 28.45030744034848 22.904951845203726 32-33 22.26273283187414 26.109715048858433 28.520386486941085 23.107165632326343 34-35 23.02759048283345 26.259874402874516 28.190725221749354 22.521809892542684 36-37 23.003079248962024 25.91418538055934 28.172656965600357 22.910078404878277 38-39 22.5817084483537 26.19069946609585 28.24511860668312 22.982473478867323 40-41 22.954000582303095 26.034647876488854 28.338844249083095 22.672507292124955 42-43 22.741212781949187 25.99083185205629 28.16185849426633 23.106096871728194 44-45 22.65099455880016 26.357983898180898 28.15611768622957 22.83490385678937 46-47 22.98177922310913 26.56913768353364 27.603386253012157 22.845696840345074 48-49 22.94484983125546 26.522638383578617 27.584040284197197 22.948471500968726 50-51 22.965351632199084 26.674672981886417 27.513764012455265 22.846211373459234 52-53 23.127984623059177 26.568314058351866 27.45618809859141 22.84751321999755 54-55 22.813819077702554 26.619360004132336 27.608370005962694 22.958450912202412 56-57 22.783738040757363 26.907871336922685 27.446498697575628 22.861891924744324 58-59 22.82315118497821 26.45238119969131 27.495576386395452 23.228891228935023 60-61 22.59861089783522 26.53746030845917 27.770786071472987 23.093142722232624 62-63 22.72894556959005 26.646099170328906 27.79323771038289 22.831717549698155 64-65 22.547619248142254 26.60748825836372 27.650128715845383 23.194763777648642 66-67 22.436009902566003 26.780270886102244 27.562375975806702 23.221343235525048 68-69 22.717175355327836 26.418132057393702 27.76047385092022 23.10421873635824 70-71 22.690380996418483 26.441808957417916 27.47159729773208 23.396212748431523 72-73 22.647306131444445 26.582441600444707 27.426432914032876 23.343819354077965 74-75 22.57357230728977 26.827635888316863 27.279403796624713 23.31938800776866 76-77 22.755419303852996 26.56560084333815 27.329096990905477 23.349882861903378 78-79 22.80348975744176 26.782187167690942 27.216582130797967 23.197740944069334 80-81 22.806825959261865 26.77376629837015 27.15586527972026 23.263542462647727 82-83 22.803399904884415 26.806395989648458 27.069423708485896 23.32078039698123 84-85 22.79933019767029 26.701560392393 27.082584156901056 23.416525253035655 86-87 22.936551647474342 26.67199459235193 27.01314464953309 23.378309110640636 88-89 22.87495065533644 26.712800300532162 26.8769362340602 23.535312810071197 90-91 22.777925350444526 26.73200547884585 26.928083689988075 23.56198548072155 92-93 22.838580569341087 26.36834706801722 27.048011079458277 23.745061283183418 94-95 22.72489379683679 26.258868981937738 27.194888770963367 23.821348450262104 96-97 23.004622206299825 26.07142786952031 27.06389807842786 23.860051845752007 98-99 22.79642887223111 26.130323703641317 26.794968977231253 24.27827844689632 100-101 23.201687213540424 25.6272112361003 27.08132290580661 24.089778644552666 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 535.0 1 381.5 2 335.5 3 464.5 4 391.0 5 347.0 6 639.0 7 1387.5 8 2175.5 9 2439.0 10 2088.0 11 1686.5 12 1651.0 13 1682.0 14 1726.5 15 1856.5 16 1949.0 17 2096.5 18 2278.0 19 2450.5 20 2810.5 21 3303.5 22 3889.5 23 4711.0 24 5792.0 25 7119.0 26 8854.0 27 11080.0 28 13244.5 29 15465.5 30 18035.0 31 21227.5 32 25109.0 33 27372.0 34 28722.0 35 31050.5 36 34153.0 37 37580.5 38 40978.5 39 43627.5 40 46181.5 41 50337.5 42 54086.0 43 56769.5 44 59968.5 45 63294.5 46 64829.0 47 66324.5 48 68028.5 49 68117.0 50 67421.0 51 66301.5 52 65267.0 53 65152.0 54 65323.5 55 62025.5 56 56682.5 57 52008.5 58 48355.0 59 43655.5 60 36650.0 61 30819.5 62 25761.5 63 20142.5 64 15343.5 65 12020.0 66 9188.0 67 7025.5 68 5406.5 69 4085.5 70 3184.5 71 2365.5 72 1843.5 73 1430.5 74 1047.5 75 774.0 76 552.5 77 377.0 78 249.5 79 151.5 80 78.5 81 49.0 82 25.5 83 16.5 84 15.5 85 11.0 86 9.0 87 9.5 88 7.5 89 3.0 90 3.0 91 2.5 92 1.5 93 2.5 94 1.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29951684312054444 2 0.03682906262175767 3 0.04379065372708991 4 0.046597746914723875 5 0.06074549658039908 6 0.05951037557784014 7 0.028744634241371837 8 0.03188857861152188 9 0.03952387208188628 10-11 0.03230964258966698 12-13 0.03458338807165049 14-15 0.028127073740092365 16-17 0.02203568152292665 18-19 0.026302463168130286 20-21 0.04056249656131085 22-23 0.03298334495469913 24-25 0.054345324112593636 26-27 0.04685038530161093 28-29 0.03893438251248314 30-31 0.03896245344435948 32-33 0.0336009054559786 34-35 0.03301141588657547 36-37 0.03216928793028528 38-39 0.020098787223459213 40-41 0.019228588335292684 42-43 0.020351425610346273 44-45 0.009038840064181378 46-47 0.016589920738916752 48-49 0.01434424618880958 50-51 0.006063321285289372 52-53 0.005249264260875522 54-55 0.007186158560342959 56-57 0.0056422573071442765 58-59 0.007607222538488055 60-61 0.006933520173455902 62-63 0.004182568849574613 64-65 0.008252853971643867 66-67 0.00266673852825227 68-69 0.007607222538488055 70-71 0.014231962461304221 72-73 0.014568813643820296 74-75 0.011172230886783194 76-77 0.010358173862369344 78-79 0.005838753830278654 80-81 0.006680881786568845 82-83 0.011845933251815348 84-85 0.00446327816833801 86-87 0.0011789791388062667 88-89 0.02026721281471725 90-91 0.030035897107683465 92-93 0.013951253142540823 94-95 0.023411157184867297 96-97 0.019228588335292684 98-99 0.0141477496656752 100-101 0.10874679008893995 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1781202.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.50478141951506 #Duplication Level Percentage of deduplicated Percentage of total 1 80.61024025946143 47.96694726813846 2 11.07568403617706 13.181123152886567 3 3.408427923917126 6.084532757905803 4 1.5449521295014468 3.677281550783916 5 0.8496470875516302 2.5279032114243667 6 0.5556732293081507 1.9839128430394544 7 0.3681373399544225 1.5334152362444735 8 0.24935887289047037 1.1870436181091262 9 0.1766421313518303 0.9459946274011137 >10 1.0473815593667486 11.458442143896077 >50 0.07283723616032314 2.960563903949419 >100 0.03674613553794784 3.8496421583787113 >500 0.003132843135701508 1.2024599824247806 >1k 0.001044281045233836 1.1013417183760932 >5k 9.493464047580326E-5 0.3393958270416274 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6008 0.3373003174260977 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4787 0.26875110178407613 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3314 0.18605413647637944 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.1228372750535874E-4 2 0.0 0.0 0.0 0.0 1.1228372750535874E-4 3 0.0 0.0 0.0 0.0 1.1228372750535874E-4 4 0.0 0.0 0.0 0.0 1.1228372750535874E-4 5 0.0 0.0 0.0 0.0 1.1228372750535874E-4 6 0.0 0.0 0.0 0.0 1.1228372750535874E-4 7 5.614186375267937E-5 0.0 0.0 0.0 1.1228372750535874E-4 8 5.614186375267937E-5 0.0 0.0 0.0 1.1228372750535874E-4 9 5.614186375267937E-5 0.0 0.0 0.0 1.1228372750535874E-4 10-11 8.421279562901906E-5 0.0 0.0 2.8070931876339685E-5 1.1228372750535874E-4 12-13 1.1228372750535874E-4 0.0 0.0 5.614186375267937E-5 2.8070931876339683E-4 14-15 1.1228372750535874E-4 2.8070931876339685E-5 0.0 1.1228372750535874E-4 3.0878025063973654E-4 16-17 1.1228372750535874E-4 5.614186375267937E-5 0.0 1.1228372750535874E-4 3.6492211439241595E-4 18-19 1.4035465938169842E-4 5.614186375267937E-5 0.0 1.6842559125803812E-4 3.929930462687556E-4 20-21 2.5263838688705713E-4 5.614186375267937E-5 0.0 1.6842559125803812E-4 3.929930462687556E-4 22-23 3.3685118251607624E-4 5.614186375267937E-5 0.0 1.6842559125803812E-4 4.2106397814509525E-4 24-25 3.929930462687556E-4 5.614186375267937E-5 0.0 2.5263838688705713E-4 4.4913491002143496E-4 26-27 3.929930462687556E-4 5.614186375267937E-5 0.0 7.017732969084921E-4 5.052767737741144E-4 28-29 3.929930462687556E-4 5.614186375267937E-5 0.0 0.00269480946012861 5.052767737741144E-4 30-31 5.052767737741144E-4 5.614186375267937E-5 0.0 0.009628329633584513 5.333477056504541E-4 32-33 5.052767737741144E-4 5.614186375267937E-5 0.0 0.022119894318555672 5.614186375267937E-4 34-35 6.175605012794731E-4 5.614186375267937E-5 0.0 0.04398715025022429 6.737023650321525E-4 36-37 7.298442287848318E-4 5.614186375267937E-5 0.0 0.08174255362390116 6.737023650321525E-4 38-39 7.298442287848318E-4 5.614186375267937E-5 0.0 0.13903532558351045 6.737023650321525E-4 40-41 7.579151606611715E-4 5.614186375267937E-5 0.0 0.19958432564077516 6.737023650321525E-4 42-43 7.859860925375112E-4 5.614186375267937E-5 0.0 0.26515802250390463 6.737023650321525E-4 44-45 8.982698200428699E-4 5.614186375267937E-5 0.0 0.34555317139774155 7.298442287848319E-4 46-47 0.0010386244794245683 5.614186375267937E-5 0.0 0.445092695831242 8.421279562901905E-4 48-49 0.0010666954113009081 5.614186375267937E-5 0.0 0.5502744775718869 8.982698200428699E-4 50-51 0.0010666954113009081 5.614186375267937E-5 0.0 0.6640178935348152 8.982698200428699E-4 52-53 0.0010666954113009081 5.614186375267937E-5 0.0 0.7766665431545664 8.982698200428699E-4 54-55 0.0011228372750535873 5.614186375267937E-5 0.0 0.9045015669194174 8.982698200428699E-4 56-57 0.0011228372750535873 5.614186375267937E-5 0.0 1.0532494349321413 8.982698200428699E-4 58-59 0.0011228372750535873 1.1228372750535874E-4 0.0 1.215359066518003 8.982698200428699E-4 60-61 0.0011228372750535873 1.1228372750535874E-4 0.0 1.3806687843377674 8.982698200428699E-4 62-63 0.0011228372750535873 1.1228372750535874E-4 0.0 1.5473820487513488 8.982698200428699E-4 64-65 0.0011228372750535873 1.1228372750535874E-4 0.0 1.726727232509283 8.982698200428699E-4 66-67 0.001263191934435286 1.1228372750535874E-4 0.0 1.9374557181049652 8.982698200428699E-4 68-69 0.001347404730064305 1.1228372750535874E-4 0.0 2.1706409492017187 9.544116837955493E-4 70-71 0.0013754756619406446 1.1228372750535874E-4 0.0 2.4178055043728897 9.544116837955493E-4 72-73 0.001431617525693324 1.1228372750535874E-4 0.0 2.6714825157393713 0.0010105535475482287 74-75 0.0014596884575696636 1.1228372750535874E-4 0.0 2.927770123770353 0.0010386244794245683 76-77 0.0014596884575696636 1.1228372750535874E-4 0.0 3.211567245040147 0.0010666954113009081 78-79 0.0014596884575696636 1.1228372750535874E-4 0.0 3.524782702916345 0.0010666954113009081 80-81 0.0017123268444567208 1.1228372750535874E-4 0.0 3.8558512734658956 0.0010666954113009081 82-83 0.0017403977763330604 1.1228372750535874E-4 0.0 4.197839436515342 0.0012351210025589462 84-85 0.0017403977763330604 1.1228372750535874E-4 0.0 4.53775035060594 0.0012351210025589462 86-87 0.0017403977763330604 1.1228372750535874E-4 0.0 4.896889853031829 0.001263191934435286 88-89 0.0017403977763330604 1.1228372750535874E-4 0.0 5.282949379127128 0.0012912628663116256 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2955 0.0 57.095818 1 GTATCAA 5295 0.0 41.40477 1 ATCAACG 6660 0.0 32.397083 3 TATCAAC 6715 0.0 32.273277 2 TCAACGC 6720 0.0 32.037098 4 CAACGCA 6845 0.0 31.590914 5 AACGCAG 7070 0.0 30.650185 6 GTACATG 5815 0.0 27.129187 1 CGCAGAG 8060 0.0 26.758518 8 TACATGG 5775 0.0 26.663507 2 ACGCAGA 8145 0.0 26.654245 7 TGGTATC 1305 0.0 26.220816 2 GTGGTAT 1440 0.0 24.823174 1 ACATGGG 6070 0.0 24.663012 3 GCAGAGT 9050 0.0 23.095808 9 GAGTACT 5495 0.0 22.561993 12-13 GTACTTT 5850 0.0 21.196121 14-15 CAGAGTA 8725 0.0 21.178782 10-11 GTACTAG 185 6.366874E-6 20.609941 1 AGTACTT 5715 0.0 20.488274 12-13 >>END_MODULE