FastQCFastQC Report
Thu 26 May 2016
SRR938505_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938505_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1781202
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57830.3246683980817448No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51790.29075871237512646No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34970.19632809754311975No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31400.054.840121
GTATCAA53700.038.0902371
TGGTATC14800.031.819472
GTGGTAT16050.031.4159561
TATCAAC67800.029.9583322
ATCAACG68900.029.2614063
TCAACGC69550.028.9879364
CAACGCA71850.028.0599985
AACGCAG74300.027.1035066
GTACATG62400.025.0801871
TATACCG1151.273053E-424.8085845
TACATGG61850.024.38032
ACGCAGA86750.022.9947287
TAAGGTG8100.022.894345
CGCAGAG87200.022.7671288
ACATGGG64800.022.6008243
ACCGTGC3200.022.263318
GAGTACT57600.020.86154612-13
ATACCGT1856.561333E-620.5384046
AGGACCG6100.020.267125
Produced by FastQC (version 0.11.5)