##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938505_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1781202 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.782643405969676 31.0 31.0 34.0 30.0 34.0 2 32.0137637393176 31.0 31.0 34.0 30.0 34.0 3 32.07805347175671 33.0 31.0 34.0 30.0 34.0 4 35.75771417278894 37.0 35.0 37.0 35.0 37.0 5 35.63067131072164 37.0 35.0 37.0 33.0 37.0 6 35.665630287861795 37.0 35.0 37.0 33.0 37.0 7 35.598578937144694 37.0 35.0 37.0 33.0 37.0 8 35.579408174929064 37.0 35.0 37.0 33.0 37.0 9 37.14521598336404 39.0 37.0 39.0 33.0 39.0 10-11 37.079562284345066 39.0 37.0 39.0 33.0 39.0 12-13 36.95933223744416 39.0 37.0 39.0 33.0 39.0 14-15 38.00269845868127 40.0 37.0 41.0 33.0 41.0 16-17 37.91511518626186 40.0 37.0 41.0 33.0 41.0 18-19 37.90838518034451 40.0 37.0 41.0 33.0 41.0 20-21 37.9001598358861 40.0 37.0 41.0 32.5 41.0 22-23 37.92773419297755 40.0 37.0 41.0 33.0 41.0 24-25 37.853662302198174 40.0 37.0 41.0 32.0 41.0 26-27 37.677860512170994 40.0 37.0 41.0 32.0 41.0 28-29 37.53992865491954 39.5 36.5 41.0 32.0 41.0 30-31 37.28759483764335 39.0 36.0 41.0 31.0 41.0 32-33 37.065367936932475 39.0 36.0 40.5 31.0 41.0 34-35 36.88863643764155 39.0 35.5 40.5 30.0 41.0 36-37 36.90109375578963 39.0 35.5 41.0 30.5 41.0 38-39 36.75516168295342 39.0 35.0 41.0 30.0 41.0 40-41 36.56880606466869 39.0 35.0 40.0 30.0 41.0 42-43 36.33440227441918 39.0 35.0 40.0 30.0 41.0 44-45 36.051426227906774 38.0 35.0 40.0 29.5 41.0 46-47 35.68535039821424 38.0 35.0 40.0 28.5 41.0 48-49 35.27680184504621 38.0 34.0 40.0 27.0 41.0 50-51 34.89096071080091 38.0 34.0 40.0 26.0 41.0 52-53 34.35760121535907 38.0 33.0 40.0 24.0 41.0 54-55 34.01684227841648 37.0 33.0 40.0 23.5 41.0 56-57 33.64009837177366 37.0 32.5 40.0 23.0 41.0 58-59 33.15879473524059 36.5 31.5 39.0 22.0 41.0 60-61 32.73751236524549 36.0 31.0 39.0 20.5 40.0 62-63 32.2373568522829 35.5 31.0 39.0 18.5 40.0 64-65 31.72516592727832 35.0 30.0 38.0 17.5 40.0 66-67 31.213536982329913 35.0 29.5 38.0 13.5 40.0 68-69 30.630345968621192 34.0 29.0 37.5 9.0 39.5 70-71 30.055427177827106 34.0 28.5 37.0 7.5 39.0 72-73 29.447811365583465 34.0 28.0 36.0 6.0 39.0 74-75 28.81693710202436 33.0 26.5 36.0 2.0 38.5 76-77 27.568745431455838 31.5 26.0 34.5 2.0 36.0 78-79 28.186491200885694 33.0 26.0 35.0 2.0 37.0 80-81 27.87696566700464 33.0 26.0 35.0 2.0 36.5 82-83 27.368065778053246 33.0 26.0 35.0 2.0 36.0 84-85 26.7623214548378 32.0 25.0 35.0 2.0 36.0 86-87 26.10476773549547 32.0 23.5 34.5 2.0 35.0 88-89 25.345888338324343 31.0 20.5 34.0 2.0 35.0 90-91 24.781687871448607 31.0 18.5 34.0 2.0 35.0 92-93 23.986982386051665 31.0 11.0 34.0 2.0 35.0 94-95 23.18141597640245 30.5 2.0 34.0 2.0 35.0 96-97 22.19018533552062 30.0 2.0 34.0 2.0 35.0 98-99 21.012062360136582 29.0 2.0 34.0 2.0 35.0 100-101 17.943873855969173 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.27852843652991055 1101 2 0.31007306455872197 1101 3 0.2646482082001782 1101 4 0.2382836306910292 1101 5 0.2269415450354515 1101 6 0.21663561923691077 1101 7 0.27337555945297254 1101 8 0.2046354075866006 1101 9 0.2945593850229855 1101 10-11 0.40101896851973606 1101 12-13 0.29473051190892363 1101 14-15 0.4324313351484079 1101 16-17 0.4938274306150774 1101 18-19 0.5822702980816601 1101 20-21 0.5395835629413526 1101 22-23 0.45278143440217633 1101 24-25 0.43266489588224744 1101 26-27 0.458825722376055 1101 28-29 0.5188586266941684 1101 30-31 0.4407501222442747 1101 32-33 0.6203136691953475 1101 34-35 0.3996535088184032 1101 36-37 0.5984654201808723 1101 38-39 0.8061035388763784 1101 40-41 0.7584740265862848 1101 42-43 0.8251226394703721 1101 44-45 0.9822346091552063 1101 46-47 1.112853989200005 1101 48-49 1.144305610734044 1101 50-51 1.0479798431730316 1101 52-53 1.2531326504811489 1101 54-55 1.1656802358888072 1101 56-57 1.3686417339528063 1101 58-59 1.3005562911753046 1101 60-61 1.3161219042464438 1101 62-63 1.4275855172261274 1101 64-65 1.5480774698198765 1101 66-67 1.6746828845103465 1101 68-69 1.6624171725424581 1101 70-71 1.8641126013620486 1101 72-73 1.7915548732350075 1101 74-75 1.8570721437974562 1101 76-77 1.7805384761671412 1101 78-79 2.185671984024669 1101 80-81 2.4754729752965687 1101 82-83 2.5549240823648915 1101 84-85 2.6894414406877267 1101 86-87 2.9712809547223706 1101 88-89 3.226654170513733 1101 90-91 3.236949452773466 1101 92-93 3.6589773705277366 1101 94-95 3.567890503440836 1101 96-97 3.944057179806574 1101 98-99 4.314529168810477 1101 100-101 4.68628454236811 1102 1 0.2797665564384104 1102 2 0.21483408478844268 1102 3 0.23922926199355032 1102 4 0.09671106980582067 1102 5 0.05470238187752585 1102 6 0.10583604572295258 1102 7 0.12492376659388071 1102 8 0.17525826025404712 1102 9 0.21678714977750246 1102 10-11 0.2100138117131678 1102 12-13 0.24124633238626103 1102 14-15 0.44486099855608785 1102 16-17 0.3311985568887863 1102 18-19 0.3911656796512304 1102 20-21 0.5058875183393141 1102 22-23 0.39606432994113305 1102 24-25 0.3897434057208429 1102 26-27 0.26178464658293166 1102 28-29 0.28043644942011525 1102 30-31 0.3103881866298863 1102 32-33 0.12878935310799022 1102 34-35 0.24711906115464188 1102 36-37 0.39581717423473606 1102 38-39 0.45967397476145067 1102 40-41 0.49211073194447863 1102 42-43 0.4191218106797976 1102 44-45 0.3714639115965568 1102 46-47 0.4305099303349351 1102 48-49 0.4195740135830093 1102 50-51 0.5991265901024647 1102 52-53 0.6766733424436069 1102 54-55 0.5189110162386967 1102 56-57 0.7856164389536815 1102 58-59 0.9452883206213443 1102 60-61 0.9654717224292426 1102 62-63 0.8285180248309771 1102 64-65 0.9146242871998886 1102 66-67 0.8059247768682241 1102 68-69 0.939055072453332 1102 70-71 1.0718288307634438 1102 72-73 0.902450531068105 1102 74-75 1.0756177007673493 1102 76-77 1.178815746805089 1102 78-79 1.6393314794350822 1102 80-81 1.7796399613999725 1102 82-83 1.8280333743095802 1102 84-85 1.8315997950757392 1102 86-87 1.9946696341996066 1102 88-89 2.118829326992703 1102 90-91 2.29886068302088 1102 92-93 2.651055902318813 1102 94-95 2.6925008891256574 1102 96-97 3.0238441461797656 1102 98-99 3.499016127517187 1102 100-101 3.9388843071050665 1103 1 0.31803168904321666 1103 2 0.3183226210341843 1103 3 0.42059143705883884 1103 4 0.2748891895319474 1103 5 0.17215147998518177 1103 6 0.30841563106423564 1103 7 0.32982144945889047 1103 8 0.20274303620871592 1103 9 0.3370786044197942 1103 10-11 0.4087806479993361 1103 12-13 0.35232956572323104 1103 14-15 0.5938594841726541 1103 16-17 0.628392776564219 1103 18-19 0.5487398427297947 1103 20-21 0.5370259047509265 1103 22-23 0.43391685722890827 1103 24-25 0.467806823735593 1103 26-27 0.3880983421276838 1103 28-29 0.49938789931392336 1103 30-31 0.5395378218303222 1103 32-33 0.6674751121285212 1103 34-35 0.6597067317634071 1103 36-37 0.8886339595067199 1103 38-39 0.8566209841750165 1103 40-41 0.8246183199038484 1103 42-43 0.887245643609937 1103 44-45 0.8978910254769019 1103 46-47 1.1630757514708492 1103 48-49 1.2621057290072528 1103 50-51 1.2047509845095163 1103 52-53 1.281533005300787 1103 54-55 1.3705737899987938 1103 56-57 1.4076718936216466 1103 58-59 1.5525965040670826 1103 60-61 1.464628704956084 1103 62-63 1.59302017127699 1103 64-65 1.4794050866146655 1103 66-67 1.5330359300455356 1103 68-69 1.6403148662148404 1103 70-71 1.529709881078194 1103 72-73 1.668402892158717 1103 74-75 1.8851472472865147 1103 76-77 1.8140837156703924 1103 78-79 2.083676123587061 1103 80-81 2.40975742236931 1103 82-83 2.7153321249432487 1103 84-85 2.665343273922499 1103 86-87 2.8669491983651874 1103 88-89 3.1167635732340173 1103 90-91 3.3000925633589198 1103 92-93 3.496248216181197 1103 94-95 3.3108975998335026 1103 96-97 3.722132283295636 1103 98-99 3.7681365017495665 1103 100-101 4.254616890091352 1104 1 0.26170685438631835 1104 2 0.26022265299641845 1104 3 0.24942506133047004 1104 4 0.11369011397814432 1104 5 0.059655475264605684 1104 6 0.10392247835850554 1104 7 0.1864622185194449 1104 8 0.1431912450942221 1104 9 0.2080937741884341 1104 10-11 0.27397070036823123 1104 12-13 0.2870202696300055 1104 14-15 0.45100550241571113 1104 16-17 0.4472277530842703 1104 18-19 0.5211195540862192 1104 20-21 0.4878034475926185 1104 22-23 0.5185755989002843 1104 24-25 0.41752715417592867 1104 26-27 0.44607414917413024 1104 28-29 0.4302701799423474 1104 30-31 0.4215658256657875 1104 32-33 0.4759045370541486 1104 34-35 0.5848966612006308 1104 36-37 0.7802106811648173 1104 38-39 0.6743434290401566 1104 40-41 0.5174538619095941 1104 42-43 0.6976352140866666 1104 44-45 0.8017646025427752 1104 46-47 0.7895318771561932 1104 48-49 0.7952794560497622 1104 50-51 0.9167976739595147 1104 52-53 1.0844501739384569 1104 54-55 1.2144308942202642 1104 56-57 1.2551690047272928 1104 58-59 1.4523463381093222 1104 60-61 1.4759968115624318 1104 62-63 1.5515460975671544 1104 64-65 1.6413061020303026 1104 66-67 1.5598645387048435 1104 68-69 1.6276098776474903 1104 70-71 1.8027515795022104 1104 72-73 1.923246400136982 1104 74-75 1.9925821549648255 1104 76-77 1.8080096616296295 1104 78-79 2.2216312781121736 1104 80-81 2.376021897573988 1104 82-83 2.607275182991586 1104 84-85 2.724310524924391 1104 86-87 2.77874940980767 1104 88-89 2.7981824506219652 1104 90-91 3.019345507572524 1104 92-93 3.2972408894533913 1104 94-95 3.3722706237068323 1104 96-97 3.720938616735509 1104 98-99 4.088517344077399 1104 100-101 4.44535091512024 1105 1 0.25277147686882273 1105 2 0.2861035349883565 1105 3 0.2771059388591226 1105 4 0.20141038586262283 1105 5 0.06274747412295056 1105 6 0.09964036825868305 1105 7 0.17742348073621628 1105 8 0.2175216643727893 1105 9 0.2897748114556862 1105 10-11 0.305515255102776 1105 12-13 0.3462966479332792 1105 14-15 0.5018665345384363 1105 16-17 0.5432525231370491 1105 18-19 0.5434318641764193 1105 20-21 0.3493500225505457 1105 22-23 0.28084008724935217 1105 24-25 0.2862992577100343 1105 26-27 0.42045342966923016 1105 28-29 0.3505987951144576 1105 30-31 0.46469568609419554 1105 32-33 0.4727080033816051 1105 34-35 0.47933587890436513 1105 36-37 0.43262199981864313 1105 38-39 0.5510052683126005 1105 40-41 0.49251261869702034 1105 42-43 0.6379506801886166 1105 44-45 0.5440074439634586 1105 46-47 0.6749677403494871 1105 48-49 0.681084421101076 1105 50-51 0.8015490690294698 1105 52-53 0.9299138034576799 1105 54-55 1.0141076878438824 1105 56-57 1.0657351840737945 1105 58-59 1.0926768384317285 1105 60-61 1.081893808359439 1105 62-63 1.253400915153911 1105 64-65 1.2738928042622923 1105 66-67 1.537917660008688 1105 68-69 1.6091302660471847 1105 70-71 1.3969656362634382 1105 72-73 1.5817916513787722 1105 74-75 1.632289557321677 1105 76-77 1.6134599482781802 1105 78-79 1.8020750753804116 1105 80-81 1.9625062508264897 1105 82-83 2.114924723566947 1105 84-85 2.3030048322403047 1105 86-87 2.484557170284347 1105 88-89 2.692499368465821 1105 90-91 2.7710336273670713 1105 92-93 3.124662426678942 1105 94-95 3.1466269417792603 1105 96-97 3.438918516687469 1105 98-99 3.9514258079911606 1105 100-101 4.213570070596397 1106 1 0.15873620706678082 1106 2 0.10637430891602406 1106 3 0.08839787502466834 1106 4 0.05401121722988478 1106 5 0.002200505082548432 1106 6 0.140997412143534 1106 7 0.10977279764906456 1106 8 0.0762159790832797 1106 9 0.11399823561170308 1106 10-11 0.22524605046552182 1106 12-13 0.1723390223409993 1106 14-15 0.023381862760885497 1106 16-17 0.05727092306714354 1106 18-19 0.2474208934097959 1106 20-21 0.49237791490435256 1106 22-23 0.19908228582522014 1106 24-25 0.11323996171510942 1106 26-27 0.08957345006999873 1106 28-29 0.1632857056274517 1106 30-31 0.22489250721113052 1106 32-33 0.27790601038277885 1106 34-35 0.16250327760934624 1106 36-37 0.21660159547670332 1106 38-39 0.303969087252149 1106 40-41 0.35531562103490444 1106 42-43 0.3202143994425555 1106 44-45 -0.17370708071095464 1106 46-47 -0.22433419290930345 1106 48-49 0.17846878847196024 1106 50-51 0.16118272888220275 1106 52-53 0.34219938578033293 1106 54-55 0.3791429166303715 1106 56-57 0.42785741753070994 1106 58-59 0.7813514656806859 1106 60-61 0.688434806963123 1106 62-63 0.8461557866415035 1106 64-65 0.5791055233610969 1106 66-67 0.8641441585472833 1106 68-69 0.3226487221507348 1106 70-71 0.6654163899686587 1106 72-73 1.25291519198327 1106 74-75 1.3653309669724543 1106 76-77 1.3274340335869503 1106 78-79 1.5498852019401177 1106 80-81 1.5004460756722793 1106 82-83 1.7421901636904948 1106 84-85 1.7587771460341877 1106 86-87 2.077567051464097 1106 88-89 2.456931490413954 1106 90-91 2.3886590769776923 1106 92-93 2.078411604138939 1106 94-95 2.811358298876641 1106 96-97 3.1471192182316194 1106 98-99 3.7477537575884092 1106 100-101 4.245529204847408 1107 1 0.2041234947286803 1107 2 0.2204081989932405 1107 3 0.15940343312802696 1107 4 0.21371420936358732 1107 5 0.1631964593247588 1107 6 0.09446244557033623 1107 7 0.14132083971001208 1107 8 0.1853172420552056 1107 9 0.23777951963509736 1107 10-11 0.28373624880918413 1107 12-13 0.2969590396816315 1107 14-15 0.4265103526208094 1107 16-17 0.4826799623659568 1107 18-19 0.42951558538025125 1107 20-21 0.32058666060274277 1107 22-23 0.20492832997964427 1107 24-25 0.29399968197785853 1107 26-27 0.374702810462189 1107 28-29 0.46406597558580387 1107 30-31 0.43220958749731864 1107 32-33 0.28180006684134895 1107 34-35 0.44537112124494627 1107 36-37 0.3799918051396105 1107 38-39 0.40850676577836254 1107 40-41 0.4036861737061912 1107 42-43 0.5180332260444303 1107 44-45 0.5678851647838101 1107 46-47 0.6149449573765295 1107 48-49 0.7699945771146659 1107 50-51 0.7593075253982065 1107 52-53 0.8277049071253018 1107 54-55 0.7953559379724169 1107 56-57 0.6525186276172121 1107 58-59 0.7151869304177083 1107 60-61 0.8229790086465414 1107 62-63 1.022099864863371 1107 64-65 1.236679153935686 1107 66-67 1.2254208769441064 1107 68-69 1.2391193440211659 1107 70-71 1.2724784369797355 1107 72-73 1.2689996172744529 1107 74-75 1.3785409437854916 1107 76-77 1.5035353560724722 1107 78-79 1.541037805309987 1107 80-81 1.8474342791434388 1107 82-83 1.8854639017396373 1107 84-85 2.266309992289557 1107 86-87 2.4707092177824315 1107 88-89 2.8293862629961524 1107 90-91 2.8429966847877424 1107 92-93 2.9057617786538152 1107 94-95 3.0997477792062327 1107 96-97 3.6968043212776642 1107 98-99 3.7148065624876594 1107 100-101 3.876270079137182 1108 1 0.22062943209770935 1108 2 0.1841547706193083 1108 3 0.09429820484707108 1108 4 0.08162039841828772 1108 5 0.009526119526235277 1108 6 0.030910098438511113 1108 7 0.19843667743964488 1108 8 0.12965994622962995 1108 9 0.2376663125410161 1108 10-11 0.1928764066244426 1108 12-13 0.30076305533165026 1108 14-15 0.317276513076429 1108 16-17 0.3025631875331456 1108 18-19 0.30402037581276176 1108 20-21 0.3600369029402515 1108 22-23 0.3347368441822809 1108 24-25 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1.3261646969271474 2316 80-81 1.41678458374313 2316 82-83 1.4156894620781735 2316 84-85 1.663012610457173 2316 86-87 1.636798291422906 2316 88-89 1.9805501276036566 2316 90-91 2.110795639021852 2316 92-93 2.1054597297525035 2316 94-95 2.6753328633501 2316 96-97 2.6912463810740803 2316 98-99 2.8808608925882275 2316 100-101 3.5163349220172186 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 15.0 4 30.0 5 73.0 6 129.0 7 346.0 8 817.0 9 1467.0 10 2287.0 11 3628.0 12 5246.0 13 7478.0 14 9627.0 15 12911.0 16 15833.0 17 19281.0 18 19764.0 19 17649.0 20 17623.0 21 19259.0 22 20751.0 23 23338.0 24 26982.0 25 30939.0 26 36355.0 27 43082.0 28 51279.0 29 60455.0 30 70919.0 31 83416.0 32 98528.0 33 118899.0 34 146759.0 35 185428.0 36 231845.0 37 254757.0 38 136260.0 39 7745.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.693160401324725 19.05163987668994 14.808603857734193 23.446595864251144 2 16.26140329789947 20.357042157799963 38.91775246769557 24.463802076605 3 19.553671682012126 23.624185942061533 29.727543229283633 27.09459914664271 4 13.591552221477407 16.717587337090347 35.4761559890456 34.21470445238665 5 14.135490973956236 37.034739070593716 33.97991739684863 14.849852558601423 6 33.432985141494335 35.62285467903135 16.571337782014616 14.372822397459691 7 29.528767652405513 32.14935756865308 19.968650383280504 18.35322439566091 8 26.94787003383109 35.433151321411046 18.89263542259665 18.726343222161216 9 26.41519603054567 16.52440318391738 19.51367671942879 37.54672406610817 10-11 25.570569761318477 26.3833635937979 26.782307677624434 21.26375896725919 12-13 26.25204777448038 23.354173193158328 27.451181842373856 22.942597189987435 14-15 22.70245036778535 25.074359898540422 25.501262630515797 26.72192710315843 16-17 22.700738040940895 28.338588211780586 26.284441629865675 22.676232117412848 18-19 22.736275840696337 26.622696358975567 28.510887591637555 22.130140208690534 20-21 23.095499555917858 25.8465070216629 28.790867066172172 22.267126356247076 22-23 22.962920544665906 25.696411748920113 28.776887742097752 22.56377996431623 24-25 22.85078111052099 25.920540405191613 28.78142155040866 22.44725693387874 26-27 22.396561061125556 26.30837648991628 28.621552491571517 22.67350995738665 28-29 22.49539917426547 26.55288395139911 28.643494673821383 22.308222200514034 30-31 22.524508730621232 26.142262359912017 28.581401772510922 22.751827136955832 32-33 22.309279832202634 26.170196878950996 28.582564748629576 22.937958540216798 34-35 23.018415338685124 26.31076705217015 28.29017592187589 22.38064168726884 36-37 22.99961794421424 25.90691930818989 28.357325744243827 22.73613700335204 38-39 22.560469081228188 26.194495437569675 28.431508239550123 22.813527241652015 40-41 22.906775797482908 25.998229188188898 28.49109316468887 22.603901849639325 42-43 22.74159979383724 26.05359445785987 28.248902233822932 22.955903514479957 44-45 22.671599038378403 26.3063411555853 28.277530685093634 22.744529120942666 46-47 22.938695330456625 26.610569716404992 27.683160023399928 22.76757492973846 48-49 22.928028376343615 26.62314549388559 27.5835362861708 22.865289843599996 50-51 22.945488495970697 26.719428790221432 27.565851599088703 22.76923111471916 52-53 23.256963556070566 26.53494662593013 27.456487248498483 22.75160256950082 54-55 22.895332167640774 26.636767373034438 27.566645323395473 22.901255135929315 56-57 22.820713203780368 26.907363679133528 27.463954116377593 22.80796900070851 58-59 22.886679893689767 26.508223098783855 27.468726174796572 23.136370832729806 60-61 22.61270759857669 26.622106869406164 27.763948165340036 23.00123736667711 62-63 22.654168364958043 26.73332390150022 27.78775231557117 22.82475541797056 64-65 22.518220841880932 26.64905496400745 27.629067337674222 23.203656856437394 66-67 22.35273675357821 26.847913595231976 27.52389122377187 23.27545842741795 68-69 22.71373612415243 26.48813508889385 27.72892955441494 23.069199232538786 70-71 22.62160608398149 26.57390907937449 27.380892228955506 23.423592607688516 72-73 22.625676369103562 26.68543489171919 27.322897683698987 23.365991055478265 74-75 22.566418631912608 27.02273520914529 27.12221859171503 23.288627567227074 76-77 22.665116028389818 26.710137311770367 27.391924105182902 23.232822554656913 78-79 22.635220485941517 26.894029986492267 27.37903954745167 23.09170998011455 80-81 22.655724985557498 26.96231362996091 27.270167712683747 23.111793671797848 82-83 22.667935114887385 26.953298910440783 27.17882368925302 23.199942285418814 84-85 22.62861525340987 26.887011601992345 27.178149211463708 23.306223933134078 86-87 22.82228145899114 26.854224400059284 27.067735024500667 23.255759116448907 88-89 22.632791021338974 27.005234108896186 26.873216442164583 23.488758427600253 90-91 22.545652166590127 26.976941206589316 27.075499133723262 23.401907493097298 92-93 22.58367048928011 26.677964303248626 27.11977356819018 23.61859163928108 94-95 22.40172647459412 26.562287713577685 27.278714036925628 23.757271774902566 96-97 22.651389342702288 26.340808061073368 27.197589043803006 23.81021355242134 98-99 22.345247759658925 26.550666347780883 26.967295118689517 24.13679077387068 100-101 22.499823152334894 26.340336875152286 27.24234418036441 23.917495792148415 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 562.0 1 407.0 2 334.0 3 419.5 4 362.5 5 335.0 6 624.5 7 1396.5 8 2156.5 9 2422.5 10 2051.0 11 1563.0 12 1531.5 13 1643.5 14 1771.5 15 1895.5 16 2004.5 17 2120.5 18 2245.0 19 2426.0 20 2732.0 21 3238.0 22 3836.5 23 4650.5 24 5635.5 25 6868.0 26 8673.5 27 11131.0 28 13353.5 29 15389.0 30 17951.0 31 21260.0 32 25718.0 33 28271.5 34 29226.5 35 31283.5 36 34391.0 37 38016.5 38 41522.5 39 43928.0 40 46337.0 41 51257.0 42 55291.5 43 57948.5 44 61322.0 45 64812.5 46 66082.0 47 67474.5 48 70020.5 49 69731.0 50 68444.0 51 66989.0 52 65453.5 53 65689.5 54 66491.5 55 62772.5 56 56643.0 57 51343.0 58 47068.5 59 42346.5 60 35138.5 61 28912.5 62 24184.5 63 18620.0 64 13717.0 65 10455.0 66 7691.5 67 6076.5 68 4730.0 69 3442.0 70 2643.5 71 1877.5 72 1411.5 73 1158.0 74 871.0 75 656.0 76 439.0 77 253.5 78 147.5 79 83.0 80 50.5 81 25.5 82 14.0 83 6.5 84 4.5 85 4.5 86 2.0 87 1.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.614186375267937E-5 2 0.044688923547132775 3 1.1228372750535874E-4 4 0.0 5 0.10391858980620952 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0011228372750535873 26-27 0.010526599453627382 28-29 0.0 30-31 0.0 32-33 3.3685118251607624E-4 34-35 0.01353018916439573 36-37 0.0027228803920049493 38-39 0.010582741317380061 40-41 0.005501902647762578 42-43 0.004772058418977747 44-45 0.0024702420051178923 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 2.8070931876339685E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.2456745501071748E-4 68-69 2.5263838688705713E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 5.614186375267937E-5 82-83 0.0012912628663116256 84-85 0.004800129350854086 86-87 0.0014596884575696636 88-89 5.614186375267937E-5 90-91 8.982698200428699E-4 92-93 1.964965231343778E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 4.4913491002143496E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1781202.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.72087714645908 #Duplication Level Percentage of deduplicated Percentage of total 1 78.68396648775747 44.63023596548195 2 12.140965704314635 13.772924483076068 3 3.7506109417312734 6.382138273503142 4 1.6941242031358585 3.843688431876477 5 0.9224903065512056 2.616222967334515 6 0.5797145995689696 1.9729152349296148 7 0.411905633894402 1.6354554199241107 8 0.2775241955101671 1.2593132638961664 9 0.21174365006424162 1.0809257005653004 >10 1.195120298578744 12.428828649853644 >50 0.08548631767213125 3.3444350198989072 >100 0.041683743829536886 4.230971976155052 >500 0.00357236225719638 1.3958784760234613 >1k 8.93090564299095E-4 0.7890653369101978 >5k 1.9846456984424333E-4 0.6170008005713381 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5783 0.3246683980817448 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5179 0.29075871237512646 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3497 0.19632809754311975 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 5.614186375267937E-5 5 0.0 0.0 0.0 0.0 1.6842559125803812E-4 6 0.0 0.0 0.0 0.0 1.6842559125803812E-4 7 0.0 0.0 0.0 0.0 1.6842559125803812E-4 8 0.0 0.0 0.0 0.0 1.6842559125803812E-4 9 0.0 0.0 0.0 0.0 1.6842559125803812E-4 10-11 0.0 0.0 0.0 2.8070931876339685E-5 2.2456745501071748E-4 12-13 0.0 0.0 0.0 5.614186375267937E-5 3.929930462687556E-4 14-15 1.1228372750535874E-4 0.0 0.0 1.1228372750535874E-4 4.4913491002143496E-4 16-17 1.1228372750535874E-4 0.0 0.0 1.1228372750535874E-4 4.4913491002143496E-4 18-19 1.1228372750535874E-4 0.0 0.0 1.6842559125803812E-4 4.4913491002143496E-4 20-21 1.6842559125803812E-4 0.0 0.0 1.6842559125803812E-4 4.4913491002143496E-4 22-23 2.2456745501071748E-4 0.0 0.0 1.6842559125803812E-4 4.4913491002143496E-4 24-25 3.0878025063973654E-4 0.0 0.0 2.5263838688705713E-4 4.4913491002143496E-4 26-27 3.3685118251607624E-4 0.0 0.0 7.017732969084921E-4 4.4913491002143496E-4 28-29 5.052767737741144E-4 0.0 0.0 0.0027228803920049493 4.4913491002143496E-4 30-31 5.052767737741144E-4 0.0 0.0 0.009740613361089872 4.4913491002143496E-4 32-33 5.052767737741144E-4 0.0 0.0 0.022456745501071747 4.4913491002143496E-4 34-35 5.614186375267937E-4 0.0 0.0 0.04488542007026716 4.4913491002143496E-4 36-37 6.175605012794731E-4 0.0 0.0 0.08314610021771815 4.4913491002143496E-4 38-39 6.175605012794731E-4 0.0 0.0 0.1410564326786069 5.614186375267937E-4 40-41 6.737023650321525E-4 0.0 0.0 0.2023072060327801 5.614186375267937E-4 42-43 7.579151606611715E-4 0.0 0.0 0.26855460526094177 5.614186375267937E-4 44-45 7.859860925375112E-4 0.0 0.0 0.3494550309285528 5.894895694031334E-4 46-47 8.421279562901905E-4 0.0 0.0 0.4494436902720747 6.175605012794731E-4 48-49 8.421279562901905E-4 0.0 0.0 0.5547658266721012 7.017732969084921E-4 50-51 8.421279562901905E-4 0.0 0.0 0.6686495972944113 7.298442287848318E-4 52-53 8.701988881665302E-4 0.0 0.0 0.7818035236879366 7.859860925375112E-4 54-55 8.982698200428699E-4 0.0 0.0 0.910256107954067 7.859860925375112E-4 56-57 8.982698200428699E-4 0.0 0.0 1.0597618911274522 7.859860925375112E-4 58-59 8.982698200428699E-4 0.0 0.0 1.22305050185212 8.140570244138509E-4 60-61 8.982698200428699E-4 0.0 0.0 1.389398844151309 8.982698200428699E-4 62-63 8.982698200428699E-4 0.0 0.0 1.5572068749080676 8.982698200428699E-4 64-65 8.982698200428699E-4 0.0 0.0 1.7359344981647225 8.982698200428699E-4 66-67 9.544116837955493E-4 0.0 0.0 1.947533182648571 8.982698200428699E-4 68-69 0.0010105535475482287 0.0 0.0 2.1827675917722975 8.982698200428699E-4 70-71 0.0010105535475482287 0.0 0.0 2.432262034289205 9.263407519192096E-4 72-73 0.0010386244794245683 0.0 0.0 2.6904023238240242 0.0010947663431772477 74-75 0.0010666954113009081 0.0 0.0 2.9527813240721716 0.0012351210025589462 76-77 0.0010666954113009081 0.0 0.0 3.242473341035997 0.0012912628663116256 78-79 0.0010666954113009081 0.0 0.0 3.5633802342463126 0.0012912628663116256 80-81 0.0011789791388062667 0.0 0.0 3.9024770913124955 0.0013193337981879652 82-83 0.0011789791388062667 0.0 0.0 4.257181386501924 0.0014596884575696636 84-85 0.0011789791388062667 0.0 0.0 4.610201425778772 0.0014877593894460032 86-87 0.0011789791388062667 0.0 0.0 4.984162380235369 0.0015439012531986828 88-89 0.0012351210025589462 2.8070931876339685E-5 0.0 5.390854041259779 0.0015719721850750224 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3140 0.0 54.84012 1 GTATCAA 5370 0.0 38.090237 1 TGGTATC 1480 0.0 31.81947 2 GTGGTAT 1605 0.0 31.415956 1 TATCAAC 6780 0.0 29.958332 2 ATCAACG 6890 0.0 29.261406 3 TCAACGC 6955 0.0 28.987936 4 CAACGCA 7185 0.0 28.059998 5 AACGCAG 7430 0.0 27.103506 6 GTACATG 6240 0.0 25.080187 1 TATACCG 115 1.273053E-4 24.808584 5 TACATGG 6185 0.0 24.3803 2 ACGCAGA 8675 0.0 22.994728 7 TAAGGTG 810 0.0 22.89434 5 CGCAGAG 8720 0.0 22.767128 8 ACATGGG 6480 0.0 22.600824 3 ACCGTGC 320 0.0 22.26331 8 GAGTACT 5760 0.0 20.861546 12-13 ATACCGT 185 6.561333E-6 20.538404 6 AGGACCG 610 0.0 20.26712 5 >>END_MODULE