FastQCFastQC Report
Thu 26 May 2016
SRR938504_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938504_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1781744
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55230.30997719088713077No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46520.26109250262663997No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31710.17797169514812453No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28800.056.151491
GTATCAA51750.043.601851
TATCAAC67550.032.9276962
ATCAACG68350.032.4032253
TCAACGC69450.031.9584334
CAACGCA70950.031.2157945
AACGCAG73800.030.0018796
GTACATG63400.027.4636171
TACATGG62500.027.2995382
ACGCAGA82100.026.6719477
CGCAGAG83500.026.2816378
ACATGGG65250.024.6193753
GTGGTAT15350.024.2404331
TGGTATC13800.023.7635252
GCAGAGT90450.023.6320239
GAGTACT55550.021.6768912-13
AGAGTAC84100.020.95408810-11
CAGAGTA87500.020.73701110-11
GTACTTT57900.020.67605614-15
AGTACTT59000.020.08730112-13
Produced by FastQC (version 0.11.5)