##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938504_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1781744 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.930692063506317 31.0 31.0 34.0 28.0 34.0 2 31.097495487567237 31.0 31.0 34.0 27.0 34.0 3 31.14707443942564 31.0 31.0 34.0 27.0 34.0 4 34.81771511507826 37.0 35.0 37.0 32.0 37.0 5 34.799191129589886 37.0 35.0 37.0 32.0 37.0 6 34.76875241336578 37.0 35.0 37.0 32.0 37.0 7 34.67521147819215 37.0 35.0 37.0 30.0 37.0 8 34.62751719663431 37.0 35.0 37.0 30.0 37.0 9 36.08093643082283 39.0 35.0 39.0 30.0 39.0 10-11 35.90231284628993 38.0 35.0 39.0 30.0 39.0 12-13 35.69297328909204 38.0 35.0 39.0 30.0 39.0 14-15 36.82222810908863 39.0 36.0 41.0 30.5 41.0 16-17 36.743373627187744 39.0 36.0 41.0 30.0 41.0 18-19 36.72000551145395 39.0 36.0 41.0 30.0 41.0 20-21 36.63738253082373 39.0 36.0 41.0 30.0 41.0 22-23 36.467697660269934 39.0 36.0 41.0 29.5 41.0 24-25 36.30638885271958 39.0 35.5 41.0 29.0 41.0 26-27 36.077553228746666 39.0 35.0 40.5 27.5 41.0 28-29 35.82548110166219 38.5 35.0 40.0 27.0 41.0 30-31 35.52709227588251 38.0 34.0 40.0 27.0 41.0 32-33 35.209286519275494 38.0 34.0 40.0 25.5 41.0 34-35 34.87910328307546 38.0 34.0 40.0 25.0 41.0 36-37 34.62527697581695 38.0 33.5 40.0 24.5 41.0 38-39 34.35917365233165 38.0 33.0 40.0 24.0 41.0 40-41 34.046164600526225 38.0 33.0 40.0 23.0 41.0 42-43 33.604213624403954 38.0 32.5 40.0 20.5 41.0 44-45 33.10976043696513 37.0 31.5 40.0 17.5 41.0 46-47 32.587329043903054 37.0 31.0 40.0 14.5 41.0 48-49 32.053438653364346 36.5 30.0 39.5 10.0 41.0 50-51 31.313544762884007 35.5 29.5 38.5 8.5 40.0 52-53 31.296082938963174 36.0 30.0 39.0 7.5 40.0 54-55 31.23277558392227 36.0 29.5 39.0 4.0 40.0 56-57 30.76857169155614 35.5 28.5 39.0 2.0 40.0 58-59 30.261942512504604 35.0 28.0 39.0 2.0 40.0 60-61 29.673240375721765 35.0 27.5 38.5 2.0 40.0 62-63 28.913824320441094 34.0 26.0 38.0 2.0 40.0 64-65 28.20863519113857 34.0 25.5 38.0 2.0 40.0 66-67 27.472040876803852 33.5 22.5 37.0 2.0 39.0 68-69 26.59516490584506 32.5 21.0 36.0 2.0 39.0 70-71 25.71714847924281 31.5 17.5 36.0 2.0 39.0 72-73 24.793627760216957 31.0 10.5 35.0 2.0 38.0 74-75 23.83591610242549 30.5 4.5 35.0 2.0 37.0 76-77 22.866099450875097 29.5 2.0 34.0 2.0 36.5 78-79 21.86618840866028 29.0 2.0 34.0 2.0 36.0 80-81 20.876057671584693 27.5 2.0 34.0 2.0 35.0 82-83 19.8563786380086 26.0 2.0 33.0 2.0 35.0 84-85 18.871525875771155 25.0 2.0 32.5 2.0 35.0 86-87 17.869701539615118 22.5 2.0 32.0 2.0 34.5 88-89 16.78862844493934 19.0 2.0 31.5 2.0 34.0 90-91 15.637766704981185 8.5 2.0 31.0 2.0 34.0 92-93 14.490659993803824 2.0 2.0 30.5 2.0 34.0 94-95 13.278629533760181 2.0 2.0 29.5 2.0 34.0 96-97 11.9163364658447 2.0 2.0 29.0 2.0 34.0 98-99 10.324968121121778 2.0 2.0 26.0 2.0 33.0 100-101 7.330022438689284 2.0 2.0 2.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5126371134619525 1101 2 0.3296688481439922 1101 3 0.42012339151034084 1101 4 0.34345589268111354 1101 5 0.31247962994864764 1101 6 0.3932483346916342 1101 7 0.422492419216006 1101 8 0.4118843477988534 1101 9 0.5011946283108131 1101 10-11 0.4889289188028485 1101 12-13 0.54504182577314 1101 14-15 0.5709095942447888 1101 16-17 0.6858799508906728 1101 18-19 0.6492995816680391 1101 20-21 0.6620355137398022 1101 22-23 0.617743827105727 1101 24-25 0.6665379129075646 1101 26-27 0.8313782728174957 1101 28-29 0.8501264135082565 1101 30-31 1.0420630747381594 1101 32-33 1.0330228368882217 1101 34-35 0.8855905625775691 1101 36-37 0.9838010067686582 1101 38-39 1.0699105375502285 1101 40-41 1.1101488885579514 1101 42-43 1.1633714541281392 1101 44-45 1.2970447404514616 1101 46-47 1.0896142591671705 1101 48-49 1.3721147280971309 1101 50-51 1.5620144060880143 1101 52-53 1.5614467949303048 1101 54-55 1.679506776810829 1101 56-57 1.7635274494227353 1101 58-59 1.8557427371378807 1101 60-61 1.9871443022311972 1101 62-63 1.9234486332809624 1101 64-65 1.9012000926713952 1101 66-67 1.8962503524429337 1101 68-69 2.460835746062262 1101 70-71 2.7801823951481914 1101 72-73 2.800507581327416 1101 74-75 2.7722618393957283 1101 76-77 3.067060937039834 1101 78-79 3.1343573653080234 1101 80-81 3.264283716809576 1101 82-83 3.41229001481398 1101 84-85 3.5917307851798377 1101 86-87 3.687363841835019 1101 88-89 3.928772327538038 1101 90-91 3.921682677174571 1101 92-93 4.2777961448830855 1101 94-95 3.9742866935591934 1101 96-97 3.900047168176574 1101 98-99 3.6360933086696416 1101 100-101 2.624006807895582 1102 1 0.6145655537462922 1102 2 0.40145602655506707 1102 3 0.4144113678359389 1102 4 0.3147887330153125 1102 5 0.42490986545907106 1102 6 0.40743881206316956 1102 7 0.5378075481322355 1102 8 0.4938042247796943 1102 9 0.6173912161737718 1102 10-11 0.7230172957500685 1102 12-13 0.6161621173431797 1102 14-15 0.8521246956827255 1102 16-17 0.7865258537219759 1102 18-19 0.7472924595058572 1102 20-21 0.7420368060676168 1102 22-23 0.7481741331067582 1102 24-25 0.6962369767531342 1102 26-27 0.8479729368090076 1102 28-29 0.8049416073672191 1102 30-31 0.6236236454248925 1102 32-33 0.8424715481175724 1102 34-35 0.8138030578210831 1102 36-37 1.1266655623286326 1102 38-39 1.217491273772417 1102 40-41 1.352944813678647 1102 42-43 1.4358608952224756 1102 44-45 1.4885993781786837 1102 46-47 1.63981009904645 1102 48-49 1.5398869442239516 1102 50-51 1.7522282537068925 1102 52-53 1.7155661877450825 1102 54-55 1.5793920011308664 1102 56-57 1.8339682898535408 1102 58-59 1.994262819455244 1102 60-61 2.2030346801979235 1102 62-63 1.9191759560280843 1102 64-65 2.187730544940443 1102 66-67 2.3790801783246067 1102 68-69 2.304652871233351 1102 70-71 2.1481926476155273 1102 72-73 2.3543873713437264 1102 74-75 2.0966067499411913 1102 76-77 2.355047868864318 1102 78-79 2.8236881222531416 1102 80-81 3.2047594566182944 1102 82-83 3.472765898214682 1102 84-85 3.3022269998994496 1102 86-87 3.3629642684337355 1102 88-89 3.6159337033841723 1102 90-91 3.671061805036052 1102 92-93 3.429739466518024 1102 94-95 3.189872101742239 1102 96-97 3.236708354416024 1102 98-99 2.729909781149912 1102 100-101 2.070048205463265 1103 1 0.6440284221319601 1103 2 0.6625827349351141 1103 3 0.5277890336114872 1103 4 0.49081695502247413 1103 5 0.33526474857537636 1103 6 0.4873518719279417 1103 7 0.4695499600342785 1103 8 0.4462279764924659 1103 9 0.6688404845926499 1103 10-11 0.6643957855724452 1103 12-13 0.5926456411974215 1103 14-15 0.6709535271012683 1103 16-17 0.7746155023522405 1103 18-19 0.7572964839206406 1103 20-21 0.8430876254831858 1103 22-23 1.0118967487208508 1103 24-25 0.9214400340307733 1103 26-27 0.9251011655235217 1103 28-29 0.8860881823027782 1103 30-31 1.0118650081045146 1103 32-33 1.254941644479949 1103 34-35 1.0794856592539048 1103 36-37 0.980456337475637 1103 38-39 1.1012471706579703 1103 40-41 1.1893272800061112 1103 42-43 1.4764539853661702 1103 44-45 1.6111303517996092 1103 46-47 1.6501707313267246 1103 48-49 1.4505343959070203 1103 50-51 1.6160466887205835 1103 52-53 1.8264220466222767 1103 54-55 1.8742766824834511 1103 56-57 2.077014676024884 1103 58-59 2.038307004974385 1103 60-61 2.149491278800877 1103 62-63 2.294574484880904 1103 64-65 2.469813754507051 1103 66-67 2.638713221963954 1103 68-69 2.7894551159624825 1103 70-71 2.6286113145121597 1103 72-73 2.8796749093920084 1103 74-75 2.8259022537755634 1103 76-77 2.9039881063047943 1103 78-79 3.205487706396948 1103 80-81 3.014470912466315 1103 82-83 3.184586019649803 1103 84-85 3.3537150751751703 1103 86-87 3.610502668058121 1103 88-89 3.915701501039152 1103 90-91 3.578154676392119 1103 92-93 3.525241224804004 1103 94-95 3.357976059402837 1103 96-97 3.103659924097487 1103 98-99 2.966790029258279 1103 100-101 1.911926074056539 1104 1 0.5377414629038242 1104 2 0.5686518292395029 1104 3 0.46418803765755 1104 4 0.5497182933355802 1104 5 0.6517291120985647 1104 6 0.6709653173060914 1104 7 0.5982147592873162 1104 8 0.6874978177623063 1104 9 0.5386195073128519 1104 10-11 0.7108866041220949 1104 12-13 0.7412217383029258 1104 14-15 0.880850819351501 1104 16-17 0.8891891095729036 1104 18-19 0.8430263314151887 1104 20-21 0.8117150764635781 1104 22-23 0.9659350307003081 1104 24-25 0.9223503981764338 1104 26-27 0.8611811530740923 1104 28-29 0.9814196482224773 1104 30-31 0.9074610242887715 1104 32-33 1.0709002501444402 1104 34-35 1.059691074753438 1104 36-37 1.238081609806791 1104 38-39 1.339809262161232 1104 40-41 1.2060406320135826 1104 42-43 1.4950114083353583 1104 44-45 1.2731829506169134 1104 46-47 1.6323602738103915 1104 48-49 1.8358751988948825 1104 50-51 1.791902586635306 1104 52-53 1.7509863167747852 1104 54-55 2.1163635172173407 1104 56-57 2.3976885011104763 1104 58-59 2.2527094326121073 1104 60-61 2.4321943600333675 1104 62-63 2.58645901647132 1104 64-65 2.5731673181547308 1104 66-67 2.7351573551727952 1104 68-69 2.8232241791433097 1104 70-71 3.0279343770705154 1104 72-73 3.0557953575712844 1104 74-75 3.0793880925555186 1104 76-77 3.146853030052071 1104 78-79 3.37515001576514 1104 80-81 3.390093383677023 1104 82-83 3.272595667953567 1104 84-85 3.593187530824096 1104 86-87 3.378639922960083 1104 88-89 3.3792869352128214 1104 90-91 3.2955994320721675 1104 92-93 3.44673982424378 1104 94-95 3.2813731960352683 1104 96-97 2.9886972723701284 1104 98-99 2.9109777043283067 1104 100-101 2.385190935556694 1105 1 0.463371889856127 1105 2 0.34798228801477293 1105 3 0.3767614315594301 1105 4 0.4208518903449203 1105 5 0.4596722643906901 1105 6 0.45887486314689596 1105 7 0.5386778359037265 1105 8 0.27788378488323673 1105 9 0.5509290503560393 1105 10-11 0.4979179584865605 1105 12-13 0.5942193043705544 1105 14-15 0.6078779597956938 1105 16-17 0.5790249065633617 1105 18-19 0.721171472118165 1105 20-21 0.6048098424598649 1105 22-23 0.49380591688250064 1105 24-25 0.6904042489302142 1105 26-27 0.7562471070461996 1105 28-29 0.701994769656821 1105 30-31 0.9073832153874335 1105 32-33 1.0706908655166743 1105 34-35 1.091111882777021 1105 36-37 1.1389871655371948 1105 38-39 1.2241187912162985 1105 40-41 1.2707706438685165 1105 42-43 1.4160253195178143 1105 44-45 1.5950569561492145 1105 46-47 1.6913346126095803 1105 48-49 1.7866405237514158 1105 50-51 1.798808782322066 1105 52-53 1.6922043145856378 1105 54-55 1.8295594697556687 1105 56-57 2.1927481604298826 1105 58-59 2.2628844764123954 1105 60-61 2.584442337876194 1105 62-63 2.3777174049702907 1105 64-65 2.5742168640654803 1105 66-67 2.7378225286079605 1105 68-69 2.953210505758854 1105 70-71 3.120110386050886 1105 72-73 3.1725650991757917 1105 74-75 3.21156618226831 1105 76-77 3.3658645422724405 1105 78-79 3.634893176450138 1105 80-81 4.042623746633691 1105 82-83 4.299098341432444 1105 84-85 4.161643190241424 1105 86-87 4.2030623885755425 1105 88-89 4.193106845199289 1105 90-91 3.9311713886950184 1105 92-93 4.066074636820497 1105 94-95 3.9020701015140666 1105 96-97 3.473077353990792 1105 98-99 3.2355811412086766 1105 100-101 2.251195264878935 1106 1 0.233293406920275 1106 2 0.11011886456457631 1106 3 0.03275627346760146 1106 4 0.21302962911256174 1106 5 0.20771969395503476 1106 6 0.010255931291190734 1106 7 0.26280948128088255 1106 8 -0.05849020787493231 1106 9 0.24098999025067513 1106 10-11 0.2878373042682014 1106 12-13 0.4431422445750499 1106 14-15 0.5929296587810668 1106 16-17 0.14252714494253382 1106 18-19 0.5179467720229667 1106 20-21 0.45561321698997403 1106 22-23 0.4468610035293423 1106 24-25 0.622383640752254 1106 26-27 0.35728028044182025 1106 28-29 0.5904992685887507 1106 30-31 0.5264436490334461 1106 32-33 0.6560666154586485 1106 34-35 0.6337762883149978 1106 36-37 0.7243724184397777 1106 38-39 0.7839335929125468 1106 40-41 1.0434035283741423 1106 42-43 0.6471911715215839 1106 44-45 1.0899509866197334 1106 46-47 1.0639975322300828 1106 48-49 0.8405326772432318 1106 50-51 0.7528490092182132 1106 52-53 0.993679692414716 1106 54-55 0.8072365833436841 1106 56-57 1.3073463781352181 1106 58-59 1.2992710245972958 1106 60-61 1.585603570865171 1106 62-63 1.4562515992142622 1106 64-65 1.5978216422692597 1106 66-67 1.8930264650222135 1106 68-69 1.6483746794821883 1106 70-71 1.5214854932471624 1106 72-73 2.0933779363509117 1106 74-75 2.130174188599007 1106 76-77 2.539867926478287 1106 78-79 2.8552217657903505 1106 80-81 2.593745787109391 1106 82-83 2.7588832519980677 1106 84-85 2.587067555026099 1106 86-87 2.5975475613652854 1106 88-89 2.4684614640125027 1106 90-91 2.6620809487309796 1106 92-93 2.357140529197453 1106 94-95 2.2985359216685204 1106 96-97 2.2958577935574347 1106 98-99 2.246888656437891 1106 100-101 1.5056832540006377 1107 1 0.27118338506350526 1107 2 0.11236226819952222 1107 3 0.19089642167712384 1107 4 0.055132271054247894 1107 5 0.12443757859066551 1107 6 0.08537429856421141 1107 7 0.17468740933576754 1107 8 0.20477581203046213 1107 9 0.38983563344083194 1107 10-11 0.13487356659022254 1107 12-13 0.2732958192497108 1107 14-15 0.307311098521005 1107 16-17 0.3166897452986319 1107 18-19 0.4494966447420623 1107 20-21 0.5102367564289381 1107 22-23 0.49915341795005475 1107 24-25 0.580860016534821 1107 26-27 0.5340289637721582 1107 28-29 0.46722921915915805 1107 30-31 0.6052576332933981 1107 32-33 0.6104466856131197 1107 34-35 0.7071416427829647 1107 36-37 0.6448034643389988 1107 38-39 0.9355237835113144 1107 40-41 0.8032860496338401 1107 42-43 0.9036601990819406 1107 44-45 0.7051834935610444 1107 46-47 0.5842246683589565 1107 48-49 0.5961069466938937 1107 50-51 0.8622576652247496 1107 52-53 0.9764189539025381 1107 54-55 0.9601734386622631 1107 56-57 1.3429739935058187 1107 58-59 1.5442626995550484 1107 60-61 1.4556449074447642 1107 62-63 1.4680173004929529 1107 64-65 1.633589611853143 1107 66-67 1.6649483216820755 1107 68-69 1.6746378956989858 1107 70-71 1.7035673537101736 1107 72-73 1.9553670158876066 1107 74-75 2.0465708997828607 1107 76-77 2.07333306826434 1107 78-79 2.410596879403691 1107 80-81 2.379398587006161 1107 82-83 2.431411121930463 1107 84-85 2.376251282086958 1107 86-87 2.492830867476691 1107 88-89 2.7181717004753914 1107 90-91 2.682701416223699 1107 92-93 2.7104483044812984 1107 94-95 2.5078006580119983 1107 96-97 2.446169798709793 1107 98-99 2.1879848794380976 1107 100-101 1.401817740502298 1108 1 0.19036888677815966 1108 2 0.250240379641518 1108 3 0.12023167163092552 1108 4 -0.046039778339611814 1108 5 -0.13631847258244534 1108 6 0.09783233160792548 1108 7 0.06833541511833374 1108 8 0.21897303096837106 1108 9 0.2160879372284512 1108 10-11 0.12934692932699932 1108 12-13 0.2564828712682967 1108 14-15 0.18290207012656623 1108 16-17 0.39964654120635146 1108 18-19 0.14223229339216914 1108 20-21 0.11713086641874781 1108 22-23 0.25286237291765445 1108 24-25 0.4189464705089492 1108 26-27 0.22628913682401475 1108 28-29 0.24768582291423513 1108 30-31 0.2260901693976507 1108 32-33 0.34256095347881654 1108 34-35 0.45127362794060133 1108 36-37 0.7555455333406087 1108 38-39 0.6374439199642268 1108 40-41 0.6443455548121477 1108 42-43 0.7891404907614827 1108 44-45 0.809827373376514 1108 46-47 0.9263851673344092 1108 48-49 0.7557768039122124 1108 50-51 0.9715594070271898 1108 52-53 1.2480516626988774 1108 54-55 1.230759119937435 1108 56-57 1.312197271613666 1108 58-59 1.4783813239710284 1108 60-61 1.6361670066347784 1108 62-63 1.6272582893513743 1108 64-65 1.7487556889791307 1108 66-67 1.8083661531208648 1108 68-69 2.0797762210763793 1108 70-71 2.262421666024462 1108 72-73 2.1844546751085616 1108 74-75 2.1869457691599443 1108 76-77 2.621159453848474 1108 78-79 2.845731051013175 1108 80-81 2.761927904594426 1108 82-83 2.9094088259107203 1108 84-85 2.690989144989725 1108 86-87 2.701662366774311 1108 88-89 2.81566055153338 1108 90-91 2.680721426264695 1108 92-93 2.572364957656399 1108 94-95 2.4701689925636607 1108 96-97 2.281642158982109 1108 98-99 2.0950909297117715 1108 100-101 1.4380409679145503 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-0.9984457847165409 2316 48-49 -0.8415584499792885 2316 50-51 -0.9443062003847622 2316 52-53 -1.0018833597392849 2316 54-55 -1.043746207372326 2316 56-57 -1.0198151111114058 2316 58-59 -0.6104318782224212 2316 60-61 -0.6490283696271106 2316 62-63 -0.7283931712903673 2316 64-65 -0.6022676969909924 2316 66-67 -0.4887415871876968 2316 68-69 -0.4550800956071086 2316 70-71 -0.6616532221390266 2316 72-73 -0.5216885884401457 2316 74-75 -0.4226628037400637 2316 76-77 -0.42508274565545534 2316 78-79 -0.4137513506255317 2316 80-81 -0.0933477207278024 2316 82-83 0.07956529074588303 2316 84-85 0.018447146243072865 2316 86-87 0.40616085478825426 2316 88-89 0.03640697294907724 2316 90-91 0.09040885088859696 2316 92-93 -0.1324433252325221 2316 94-95 0.0041795380605691435 2316 96-97 0.0540973981576407 2316 98-99 0.0891461559626503 2316 100-101 0.18752646183330146 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6610.0 3 2731.0 4 2960.0 5 3556.0 6 4388.0 7 5579.0 8 6964.0 9 9039.0 10 11454.0 11 13758.0 12 17052.0 13 19887.0 14 22449.0 15 25264.0 16 28807.0 17 32591.0 18 33192.0 19 32962.0 20 36168.0 21 39212.0 22 43420.0 23 48174.0 24 52675.0 25 57429.0 26 63164.0 27 68630.0 28 75150.0 29 81847.0 30 89225.0 31 98873.0 32 109166.0 33 121127.0 34 132932.0 35 141234.0 36 133553.0 37 89374.0 38 20874.0 39 274.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.00983463768859 18.17407917176305 14.131657719001819 23.68442847154654 2 15.764523324169208 19.542830451695774 39.874670240245315 24.817975983889703 3 19.440835608116682 23.42158482125312 29.184974415003577 27.95260515562662 4 13.091786246100426 15.883981933379902 35.76978535220252 35.254446468317155 5 14.239550538071613 36.79256303148682 33.82466087616771 15.143225554273855 6 33.52856646284178 34.976473752749506 16.892232997169213 14.602726787239503 7 29.69753421211907 31.936214483907083 19.919105897835824 18.447145406138024 8 26.992413489525312 34.89884652700421 19.15060503431672 18.95813494915376 9 26.73176439279396 16.43844845678302 19.444202710995402 37.38558443942762 10-11 25.625691465813482 26.103741558186716 26.82237473187058 21.448192244129224 12-13 26.36581719414133 23.263117532167506 27.336863492106268 23.0342017815849 14-15 22.886160912906657 24.900470482757807 25.42700920783122 26.786359396504324 16-17 22.866412720475527 28.130739156318935 26.205512284654013 22.797335838551525 18-19 22.88548318098782 26.45944607687376 28.460616036531484 22.194454705606937 20-21 23.276264030001037 25.718101090959518 28.717025655219963 22.288609223819485 22-23 22.992785559891082 25.590320860118464 28.642927322235636 22.77396625775482 24-25 22.897940093306975 25.751416477119914 28.71968772652264 22.630955703050475 26-27 22.480582942634094 26.207265560104236 28.46673813929189 22.845413357969775 28-29 22.49654372867463 26.4843439681223 28.51614026075374 22.50297204244933 30-31 22.592159242817335 26.06310379540168 28.409901809470284 22.934835152310697 32-33 22.312883847169353 26.07414048690607 28.495926823455637 23.11704884246894 34-35 23.083001489437795 26.21983158629897 28.14265479233487 22.55451213192837 36-37 22.990508181300388 25.90242175699002 28.183147495277673 22.923922566431923 38-39 22.609120315471138 26.18542349333764 28.27567237592708 22.929783815264145 40-41 22.98317315923276 25.993783886491094 28.28780706584736 22.73523588842879 42-43 22.800622848363705 25.91374201301942 28.198118095327906 23.087517043288972 44-45 22.664689363369334 26.299640032757978 28.161276326077672 22.874394277795023 46-47 22.996388250193107 26.51127647344123 27.582591972192784 22.909743304172878 48-49 22.988399285522625 26.46005314795542 27.521552764558148 23.029994801963802 50-51 23.033965343960116 26.613768508251162 27.446420547656476 22.905845600132245 52-53 23.23338205445989 26.484450775945685 27.424437349570713 22.85772982002371 54-55 22.794256939082906 26.62346938288673 27.564649287281302 23.017624390749067 56-57 22.793270125341095 26.887448406006246 27.377238780104275 22.942042688548387 58-59 22.886953681208603 26.389972068238478 27.473902900295144 23.24917135025778 60-61 22.60303613365005 26.49028293541565 27.779371219707137 23.127309711227163 62-63 22.723215964641767 26.588024834735403 27.757352218898625 22.93140698172421 64-65 22.5624240151863 26.51032270883686 27.68417933033454 23.243073945642305 66-67 22.401099192527916 26.684296179177814 27.505294887413307 23.409309740880964 68-69 22.78956400696556 26.364255054030806 27.692482554525437 23.153698384478194 70-71 22.713919528754946 26.321707039627146 27.372623026690807 23.591750404927105 72-73 22.675767063665358 26.469127167935046 27.302818806713763 23.552286961685827 74-75 22.58191355451993 26.75723501321198 27.169970713334486 23.490880718933614 76-77 22.76839938124286 26.481699965649547 27.15341405315115 23.596486599956446 78-79 22.791081055367474 26.588977134602825 27.081686268665678 23.53825554136402 80-81 22.830789437863885 26.62730960777187 26.952671897123437 23.589229057240804 82-83 22.80552757514786 26.611131209604018 26.92513546836396 23.658205746884164 84-85 22.825194678575127 26.481871114602374 26.867285158963295 23.8256490478592 86-87 23.000580625734376 26.37982801242747 26.792271531911826 23.82731982992633 88-89 22.83715425159428 26.457532893970537 26.65360983943639 24.05170301499879 90-91 22.781795312774992 26.45601765239039 26.645459171829827 24.116727863004787 92-93 22.885637346183586 26.032873106105786 26.779695742044485 24.30179380566614 94-95 22.725129324983932 25.895759712355414 26.880818922567805 24.49829204009285 96-97 22.952528135300934 25.72443347674178 26.69575253509615 24.62728585286113 98-99 22.80099813726324 25.76622134431947 26.309942894221976 25.122837624195316 100-101 23.59446318365535 24.835175332985866 26.513640444102908 25.056721039255876 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 406.0 1 311.0 2 302.5 3 414.5 4 359.0 5 328.0 6 564.5 7 1143.5 8 1807.0 9 2106.0 10 1844.5 11 1484.5 12 1431.0 13 1510.0 14 1633.5 15 1765.0 16 1892.5 17 2079.0 18 2288.5 19 2476.0 20 2743.5 21 3187.0 22 3887.5 23 4722.0 24 5692.0 25 7020.0 26 8787.5 27 11026.0 28 13179.0 29 15166.5 30 17706.5 31 20984.5 32 24701.5 33 27028.0 34 28524.5 35 30815.5 36 33718.5 37 37169.0 38 40512.5 39 43270.0 40 46081.0 41 49798.0 42 53147.5 43 55977.5 44 58986.5 45 62294.0 46 64483.0 47 66378.5 48 68277.0 49 68158.0 50 67338.5 51 66419.0 52 65925.0 53 65743.5 54 65053.5 55 62196.0 56 57747.5 57 53285.5 58 49178.5 59 44231.0 60 37619.0 61 31726.0 62 26505.5 63 21028.5 64 16237.5 65 12696.0 66 9643.0 67 7319.5 68 5617.5 69 4220.5 70 3306.0 71 2530.0 72 1902.0 73 1440.5 74 1079.0 75 814.0 76 583.5 77 376.0 78 244.5 79 149.5 80 82.5 81 44.5 82 27.0 83 18.0 84 8.5 85 6.0 86 6.0 87 5.5 88 6.0 89 4.5 90 3.0 91 3.5 92 4.0 93 2.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3812556686033459 2 0.029353262870535832 3 0.034909616645264416 4 0.04377733277058882 5 0.06319650858933719 6 0.05427266767840947 7 0.036481110642157345 8 0.03861385249508347 9 0.03519024057328101 10-11 0.036733672177372285 12-13 0.029241013299329193 14-15 0.027416957767221327 16-17 0.022197352706112665 18-19 0.027276645803213033 20-21 0.03398355768280965 22-23 0.03333812264837148 24-25 0.041588466132059375 26-27 0.03606017475013246 28-29 0.03151406711626362 30-31 0.03451674314604118 32-33 0.02904457654971758 34-35 0.030026760297775665 36-37 0.03246818847152004 38-39 0.015799127147334297 40-41 0.013750572472813154 42-43 0.014087321186433067 44-45 0.010832083621440565 46-47 0.018857927962715182 48-49 0.016949685252202338 50-51 0.011645893012688691 52-53 0.009877962266184143 54-55 0.00844678023329951 56-57 0.009288652017349294 58-59 0.004518045241067179 60-61 0.007632970842051383 62-63 0.011617830619887031 64-65 0.007745220413258021 66-67 0.004069046956240627 68-69 0.006819161450803258 70-71 0.014059258793631409 72-73 0.011786204976696989 74-75 0.012151016083118563 76-77 0.006061476845158452 78-79 0.009288652017349294 80-81 0.010747896443035587 82-83 0.0134418861519949 84-85 0.008615154590109466 86-87 0.0025536777449510144 88-89 0.016865498073797357 90-91 0.025564839842311803 92-93 0.011028520371052184 94-95 0.0212712937436579 96-97 0.019755924532368286 98-99 0.012656139153548435 100-101 0.12355871550570677 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1781744.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.05284893223439 #Duplication Level Percentage of deduplicated Percentage of total 1 80.96202449101038 48.620002260065085 2 10.947681528999272 13.148789300384124 3 3.394669794383605 6.115787770108135 4 1.4653319867037373 3.5198944173236173 5 0.7897223314214259 2.371253793363142 6 0.49940468386524484 1.7994404421725896 7 0.3374161958995956 1.4183962687753335 8 0.2543196930150431 1.2218097684099687 9 0.18880687926878228 1.0204551898285321 >10 1.0486809267579595 11.466420194881243 >50 0.07246773916472543 2.976865866750185 >100 0.035329179554649794 3.7655431535691704 >500 0.0030142326950175565 1.1626142333644167 >1k 0.001036142488912285 1.080309766398756 >5k 9.419477171929864E-5 0.31241757460573505 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5523 0.30997719088713077 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4652 0.26109250262663997 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3171 0.17797169514812453 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.12249571206638E-4 2 5.6124785603319E-5 0.0 0.0 0.0 1.12249571206638E-4 3 5.6124785603319E-5 0.0 0.0 0.0 1.12249571206638E-4 4 5.6124785603319E-5 0.0 0.0 0.0 1.12249571206638E-4 5 5.6124785603319E-5 0.0 0.0 0.0 1.12249571206638E-4 6 5.6124785603319E-5 0.0 0.0 0.0 1.6837435680995699E-4 7 5.6124785603319E-5 0.0 0.0 0.0 1.6837435680995699E-4 8 5.6124785603319E-5 0.0 0.0 0.0 2.24499142413276E-4 9 5.6124785603319E-5 0.0 0.0 5.6124785603319E-5 2.24499142413276E-4 10-11 5.6124785603319E-5 0.0 0.0 5.6124785603319E-5 2.24499142413276E-4 12-13 5.6124785603319E-5 0.0 0.0 5.6124785603319E-5 3.92873499223233E-4 14-15 1.6837435680995699E-4 0.0 0.0 5.6124785603319E-5 4.209358920248925E-4 16-17 2.24499142413276E-4 0.0 0.0 8.418717840497849E-5 4.48998284826552E-4 18-19 2.24499142413276E-4 0.0 0.0 1.12249571206638E-4 4.48998284826552E-4 20-21 2.24499142413276E-4 0.0 0.0 1.12249571206638E-4 5.051230704298709E-4 22-23 2.24499142413276E-4 0.0 0.0 2.8062392801659497E-4 5.612478560331899E-4 24-25 2.8062392801659497E-4 0.0 0.0 6.454350344381684E-4 5.612478560331899E-4 26-27 2.8062392801659497E-4 0.0 0.0 0.0012908700688763369 5.893102488348494E-4 28-29 3.92873499223233E-4 0.0 0.0 0.003451674314604118 6.173726416365089E-4 30-31 3.92873499223233E-4 0.0 0.0 0.009008028089332699 6.454350344381684E-4 32-33 4.209358920248925E-4 0.0 0.0 0.021159044172451263 6.734974272398279E-4 34-35 5.331854632315304E-4 0.0 0.0 0.04206552680968759 6.734974272398279E-4 36-37 5.612478560331899E-4 0.0 0.0 0.07975332034231629 6.734974272398279E-4 38-39 5.612478560331899E-4 0.0 0.0 0.13627097944485853 6.734974272398279E-4 40-41 5.612478560331899E-4 0.0 0.0 0.19727862139566626 6.734974272398279E-4 42-43 5.893102488348494E-4 0.0 0.0 0.2598016325577636 6.734974272398279E-4 44-45 6.454350344381684E-4 0.0 0.0 0.3401723255417164 6.734974272398279E-4 46-47 7.296222128431469E-4 0.0 0.0 0.44046731741484746 7.015598200414874E-4 48-49 7.85746998446466E-4 0.0 0.0 0.5447752314586158 7.296222128431469E-4 50-51 7.85746998446466E-4 0.0 0.0 0.6596065428030065 7.296222128431469E-4 52-53 8.41871784049785E-4 0.0 0.0 0.7713790533320162 7.85746998446466E-4 54-55 8.97996569653104E-4 0.0 0.0 0.8938152731256567 7.85746998446466E-4 56-57 0.0010663709264630609 0.0 0.0 1.0415918336191956 7.85746998446466E-4 58-59 0.0010663709264630609 0.0 0.0 1.2032312161567542 7.85746998446466E-4 60-61 0.0010663709264630609 0.0 0.0 1.3744118122468771 7.85746998446466E-4 62-63 0.0010663709264630609 0.0 0.0 1.5435157912696773 8.41871784049785E-4 64-65 0.0010663709264630609 0.0 0.0 1.7219364847026284 8.699341768514445E-4 66-67 0.0011786204976696989 0.0 0.0 1.9325166802862812 8.97996569653104E-4 68-69 0.0011786204976696989 0.0 0.0 2.166585098644923 8.97996569653104E-4 70-71 0.0012066828904713583 0.0 0.0 2.413000969836295 8.97996569653104E-4 72-73 0.0012628076760746775 0.0 0.0 2.662896577735073 9.821837480580825E-4 74-75 0.0012908700688763369 0.0 0.0 2.911866126671396 0.0010663709264630609 76-77 0.0012908700688763369 0.0 0.0 3.184576459917923 0.0010663709264630609 78-79 0.0012908700688763369 0.0 0.0 3.4884360491742923 0.0010663709264630609 80-81 0.0012908700688763369 0.0 0.0 3.8093295108612684 0.0010944333192647203 82-83 0.0012908700688763369 0.0 0.0 4.137210508355858 0.0011224957120663799 84-85 0.0012908700688763369 2.80623928016595E-5 0.0 4.470647859625176 0.0011786204976696989 86-87 0.0012908700688763369 5.6124785603319E-5 0.0 4.810567623631678 0.0012908700688763369 88-89 0.0012908700688763369 5.6124785603319E-5 0.0 5.17484554459002 0.0013469948544796559 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2880 0.0 56.15149 1 GTATCAA 5175 0.0 43.60185 1 TATCAAC 6755 0.0 32.927696 2 ATCAACG 6835 0.0 32.403225 3 TCAACGC 6945 0.0 31.958433 4 CAACGCA 7095 0.0 31.215794 5 AACGCAG 7380 0.0 30.001879 6 GTACATG 6340 0.0 27.463617 1 TACATGG 6250 0.0 27.299538 2 ACGCAGA 8210 0.0 26.671947 7 CGCAGAG 8350 0.0 26.281637 8 ACATGGG 6525 0.0 24.619375 3 GTGGTAT 1535 0.0 24.240433 1 TGGTATC 1380 0.0 23.763525 2 GCAGAGT 9045 0.0 23.632023 9 GAGTACT 5555 0.0 21.67689 12-13 AGAGTAC 8410 0.0 20.954088 10-11 CAGAGTA 8750 0.0 20.737011 10-11 GTACTTT 5790 0.0 20.676056 14-15 AGTACTT 5900 0.0 20.087301 12-13 >>END_MODULE