FastQCFastQC Report
Thu 26 May 2016
SRR938504_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938504_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1781744
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56620.31777853608599216No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51110.28685377921856337No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34480.1935182607602439No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32400.060.420551
GTATCAA56300.038.991151
ATCAACG71050.030.2150943
TATCAAC71300.030.1108422
TCAACGC71850.029.878674
CAACGCA73400.029.2477175
AACGCAG75400.028.4719166
TGGTATC13200.028.0669142
GTGGTAT14700.026.5050721
GTACATG61250.025.1345631
TACATGG60250.024.6752342
ACGCAGA87100.024.5382697
CGCAGAG87550.024.3036488
ACATGGG61250.023.573123
GAGTACT61150.022.52429212-13
GTAAGGT8800.021.5887224
TAAGGTG9400.021.2212545
CAGAGTA93350.020.98742910-11
GTACTTT65150.020.84976814-15
GCAGAGT99200.020.7312669
Produced by FastQC (version 0.11.5)