##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938504_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1781744 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.794745485322245 31.0 31.0 34.0 30.0 34.0 2 32.04965079158398 31.0 31.0 34.0 30.0 34.0 3 32.104750177354326 33.0 31.0 34.0 30.0 34.0 4 35.76699065634569 37.0 35.0 37.0 35.0 37.0 5 35.655203553372424 37.0 35.0 37.0 33.0 37.0 6 35.687175598739216 37.0 35.0 37.0 33.0 37.0 7 35.6094876705071 37.0 35.0 37.0 33.0 37.0 8 35.58001317809966 37.0 35.0 37.0 33.0 37.0 9 37.1154273565675 39.0 37.0 39.0 33.0 39.0 10-11 37.04542852396304 39.0 37.0 39.0 33.0 39.0 12-13 36.92278155559946 39.0 37.0 39.0 33.0 39.0 14-15 37.92511550480877 40.0 37.0 41.0 33.0 41.0 16-17 38.016247564184305 40.0 37.0 41.0 33.0 41.0 18-19 38.1427609690281 40.0 37.0 41.0 33.0 41.0 20-21 38.093030760872495 40.0 37.0 41.0 33.0 41.0 22-23 37.96239723551756 40.0 37.0 41.0 33.0 41.0 24-25 37.837090513564235 40.0 37.0 41.0 32.0 41.0 26-27 37.61085964089118 40.0 37.0 41.0 32.0 41.0 28-29 37.463886506703545 39.0 36.5 41.0 32.0 41.0 30-31 37.19993977810505 39.0 36.0 41.0 31.0 41.0 32-33 37.08697433525804 39.0 36.0 41.0 31.0 41.0 34-35 36.946612981438406 39.0 36.0 41.0 30.5 41.0 36-37 36.81331465126303 39.0 35.0 41.0 30.0 41.0 38-39 36.59261936619402 39.0 35.0 40.0 30.0 41.0 40-41 36.35532377266318 39.0 35.0 40.0 30.0 41.0 42-43 36.08696339092485 38.0 35.0 40.0 29.5 41.0 44-45 35.77769449483203 38.0 35.0 40.0 28.5 41.0 46-47 35.35268703023554 38.0 34.0 40.0 27.0 41.0 48-49 34.914200917752495 38.0 34.0 40.0 26.0 41.0 50-51 34.49856881796711 38.0 33.0 40.0 24.5 41.0 52-53 33.87421733986476 37.0 33.0 40.0 23.5 41.0 54-55 33.415473547266046 37.0 32.0 39.5 22.5 41.0 56-57 32.99952799055308 36.5 31.0 39.0 21.0 40.0 58-59 32.429584160238505 36.0 31.0 39.0 18.5 40.0 60-61 31.90625561247856 35.0 30.0 39.0 16.5 40.0 62-63 31.36058406819386 35.0 29.5 38.0 12.0 40.0 64-65 30.67625034797367 34.5 28.5 38.0 8.0 40.0 66-67 29.99623823624494 34.0 28.0 37.5 6.5 39.5 68-69 29.2400998684435 34.0 27.0 37.0 2.0 39.0 70-71 28.436565241695774 33.0 26.0 36.0 2.0 39.0 72-73 27.838830101294015 32.5 25.5 35.5 2.0 38.5 74-75 27.107597387727978 32.0 24.5 35.0 2.0 37.5 76-77 25.977663177201663 30.5 23.0 34.0 2.0 36.0 78-79 26.459514947152904 32.0 24.0 35.0 2.0 36.0 80-81 26.067441226124515 32.0 22.5 35.0 2.0 36.0 82-83 25.371418396806725 31.0 20.5 34.0 2.0 36.0 84-85 24.576004465287944 31.0 17.5 34.0 2.0 35.0 86-87 23.64365335311919 30.0 9.0 34.0 2.0 35.0 88-89 22.654381325263337 29.0 3.5 34.0 2.0 35.0 90-91 21.772063775716376 29.0 2.0 34.0 2.0 35.0 92-93 20.672090378864752 28.0 2.0 33.0 2.0 35.0 94-95 19.488339795166983 26.0 2.0 33.0 2.0 34.0 96-97 18.102698255192664 24.5 2.0 32.0 2.0 34.0 98-99 16.35855263157895 10.5 2.0 32.0 2.0 34.0 100-101 12.273750044899828 2.0 2.0 27.0 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2532170031329386 1101 2 0.22873438785131128 1101 3 0.23531181625167363 1101 4 0.15533821805014725 1101 5 0.18247485767690108 1101 6 0.19169459547842393 1101 7 0.17893043457845437 1101 8 0.14423572591491762 1101 9 0.3102522357349571 1101 10-11 0.3023315715872599 1101 12-13 0.40195879582898186 1101 14-15 0.45140127035880084 1101 16-17 0.34251431197779425 1101 18-19 0.30558189631030075 1101 20-21 0.3942711129591814 1101 22-23 0.3842035228789058 1101 24-25 0.3893169132200214 1101 26-27 0.47310505861015173 1101 28-29 0.44415374082235104 1101 30-31 0.4110550548078109 1101 32-33 0.3715292950361615 1101 34-35 0.2520021302459625 1101 36-37 0.46062219935728166 1101 38-39 0.5872423454811369 1101 40-41 0.5235553602129741 1101 42-43 0.5861208997668115 1101 44-45 0.7254559347657619 1101 46-47 0.7579379632747489 1101 48-49 0.7910643532037085 1101 50-51 0.839946724369419 1101 52-53 0.8807678165830239 1101 54-55 0.740388598206728 1101 56-57 0.9903384574091518 1101 58-59 0.9418289172704917 1101 60-61 0.9228847925171699 1101 62-63 1.2476237287080387 1101 64-65 1.2965968645433499 1101 66-67 1.432371955583406 1101 68-69 1.5715618161613953 1101 70-71 1.7397345436045946 1101 72-73 1.6688383245588234 1101 74-75 1.7261228611889443 1101 76-77 1.9522394907188136 1101 78-79 2.0192102798294336 1101 80-81 2.0778894464087756 1101 82-83 2.043165038784551 1101 84-85 2.204170271126536 1101 86-87 2.121050639843272 1101 88-89 2.054085833293403 1101 90-91 2.537827928097027 1101 92-93 2.8150315201128997 1101 94-95 2.4954967816292637 1101 96-97 2.776246569951425 1101 98-99 2.7612196370843733 1101 100-101 2.324518932331582 1102 1 0.1674880225511437 1102 2 0.2623629767533515 1102 3 0.23053640389793628 1102 4 0.12200007656114309 1102 5 0.19907800664901742 1102 6 0.2867626025979746 1102 7 0.330263653473331 1102 8 0.3009764882186303 1102 9 0.28061191406281694 1102 10-11 0.3049049683012228 1102 12-13 0.3407603836951978 1102 14-15 0.5330918896905814 1102 16-17 0.351864107914011 1102 18-19 0.4745377672315527 1102 20-21 0.3757443209802247 1102 22-23 0.5025032437230408 1102 24-25 0.39491504234706554 1102 26-27 0.42082387157442014 1102 28-29 0.33784982448165124 1102 30-31 0.4236470923714748 1102 32-33 0.4063971806246798 1102 34-35 0.4900223166675133 1102 36-37 0.3985416755859603 1102 38-39 0.5354908170424224 1102 40-41 0.5762164133034915 1102 42-43 0.5979783338173021 1102 44-45 0.6236348621206247 1102 46-47 0.5619314846657062 1102 48-49 0.8207345354806748 1102 50-51 0.7607596638343068 1102 52-53 0.8050842371743769 1102 54-55 0.8358824860835838 1102 56-57 1.022385413291822 1102 58-59 1.1183408265934602 1102 60-61 0.9983444989760066 1102 62-63 1.1595605625962584 1102 64-65 0.9974649106938145 1102 66-67 1.1862130410343426 1102 68-69 1.3315998745628654 1102 70-71 1.5332613519008156 1102 72-73 1.6324085498481118 1102 74-75 1.7719383298307356 1102 76-77 1.6689630285118966 1102 78-79 1.8041923863107208 1102 80-81 1.7900002550339522 1102 82-83 2.0771188308855564 1102 84-85 2.158638233471496 1102 86-87 2.083900783990039 1102 88-89 2.148192753186702 1102 90-91 2.253572756011831 1102 92-93 2.380702169716489 1102 94-95 1.8628608701889675 1102 96-97 2.23923508455114 1102 98-99 2.125187122638696 1102 100-101 2.14984124737369 1103 1 0.27058330855608403 1103 2 0.25285705351746657 1103 3 0.23088678350245573 1103 4 0.20399337559112496 1103 5 0.1466711830301719 1103 6 0.2897716511432762 1103 7 0.3071146447250186 1103 8 0.3271157010303227 1103 9 0.30452300643590746 1103 10-11 0.3992521028220821 1103 12-13 0.3987577901834456 1103 14-15 0.5179353367615462 1103 16-17 0.36961976205708424 1103 18-19 0.4287709086066869 1103 20-21 0.38728049759454564 1103 22-23 0.4303750309784249 1103 24-25 0.422020114706676 1103 26-27 0.464810086356799 1103 28-29 0.34832591913843913 1103 30-31 0.3645792234907361 1103 32-33 0.6042235175448525 1103 34-35 0.5899790382387025 1103 36-37 0.6347085482643351 1103 38-39 0.584493816189628 1103 40-41 0.5732523998760044 1103 42-43 0.625322315879913 1103 44-45 0.7380547235657744 1103 46-47 0.6899728722583731 1103 48-49 0.7827071694601528 1103 50-51 0.9172595728058752 1103 52-53 1.233151815402934 1103 54-55 1.1684285033502348 1103 56-57 0.9688493580487432 1103 58-59 1.2171687956373987 1103 60-61 1.2510993067143765 1103 62-63 1.2907540890376232 1103 64-65 1.465364932732271 1103 66-67 1.212765395157291 1103 68-69 1.5271485843546486 1103 70-71 1.6552726649779252 1103 72-73 1.6554235711359873 1103 74-75 1.5128929785089085 1103 76-77 1.5653693219300635 1103 78-79 1.69873227752376 1103 80-81 1.8833804121138904 1103 82-83 2.0801468836117785 1103 84-85 2.1027055753455315 1103 86-87 1.9045150502210468 1103 88-89 1.8948152790499542 1103 90-91 2.0135641642056648 1103 92-93 2.376382471483719 1103 94-95 2.115628932944283 1103 96-97 2.1999849032927763 1103 98-99 2.224657880154929 1103 100-101 2.128951340669847 1104 1 0.16025028645835349 1104 2 0.25453772578637057 1104 3 0.29293160142962904 1104 4 0.19165288383887003 1104 5 0.1150807690868163 1104 6 0.09721018211121901 1104 7 0.05599419654574689 1104 8 0.026290926676139748 1104 9 0.15719963264194803 1104 10-11 0.21975474832472486 1104 12-13 0.33127413005272643 1104 14-15 0.4704563451649051 1104 16-17 0.3860530020197359 1104 18-19 0.4000438622325859 1104 20-21 0.3635254400159553 1104 22-23 0.35570960925417694 1104 24-25 0.23197965407798193 1104 26-27 0.33371764938200954 1104 28-29 0.3427937062532891 1104 30-31 0.46409680830243616 1104 32-33 0.6316122508159481 1104 34-35 0.6336842981204356 1104 36-37 0.5973213711712759 1104 38-39 0.5476590822960716 1104 40-41 0.5513491941492745 1104 42-43 0.6921757239097914 1104 44-45 0.7267101857506475 1104 46-47 0.8586392276894372 1104 48-49 0.7817812435342262 1104 50-51 0.8998926260271674 1104 52-53 0.880638587889699 1104 54-55 0.8137755310502044 1104 56-57 0.8760544623126734 1104 58-59 1.0853527358801713 1104 60-61 0.9998154598422921 1104 62-63 1.0992663828440392 1104 64-65 1.2609765106967963 1104 66-67 1.3225537549456519 1104 68-69 1.28448596375085 1104 70-71 1.3341397673744417 1104 72-73 1.5900251895611355 1104 74-75 1.8011749579673584 1104 76-77 1.7575495273455601 1104 78-79 1.8716392180777603 1104 80-81 2.0072599639346187 1104 82-83 2.0913980507452976 1104 84-85 2.3639256189185147 1104 86-87 2.1738194386430827 1104 88-89 1.9926677533730164 1104 90-91 2.251340385805417 1104 92-93 2.4846224341354457 1104 94-95 2.558151497525671 1104 96-97 2.7829214112292853 1104 98-99 2.958854892293118 1104 100-101 2.4070948202839126 1105 1 0.17754834176037448 1105 2 0.14573465399585928 1105 3 0.21208465590984815 1105 4 0.13455076708705604 1105 5 0.05971055608276288 1105 6 0.11093427349558738 1105 7 0.09710614694388653 1105 8 0.22530598838121563 1105 9 0.33748810258672535 1105 10-11 0.22965527532703334 1105 12-13 0.2656195910835848 1105 14-15 0.44213827636434644 1105 16-17 0.24790635759417512 1105 18-19 0.2676340943304183 1105 20-21 0.2862292905948891 1105 22-23 0.26268863057556757 1105 24-25 0.20858103120811222 1105 26-27 0.2723447856297625 1105 28-29 0.3737751999743679 1105 30-31 0.4753021643524775 1105 32-33 0.5064990344932028 1105 34-35 0.4663615013363014 1105 36-37 0.43310655915408347 1105 38-39 0.4215535839169462 1105 40-41 0.4235655588474643 1105 42-43 0.497865040835066 1105 44-45 0.5881412750403001 1105 46-47 0.6210401306223972 1105 48-49 0.5730763508405659 1105 50-51 0.5402004345438272 1105 52-53 0.7234737868881567 1105 54-55 0.8792364220233182 1105 56-57 1.0519576575483143 1105 58-59 1.1637146049723768 1105 60-61 0.9349975851268617 1105 62-63 1.038385725962062 1105 64-65 1.3386630160105284 1105 66-67 1.4122272023520779 1105 68-69 1.4867589453529781 1105 70-71 1.5598992347069434 1105 72-73 1.7893737804331913 1105 74-75 1.7374704499469225 1105 76-77 1.7714121885149012 1105 78-79 1.925978681703409 1105 80-81 2.042474611591434 1105 82-83 2.0207851613948016 1105 84-85 2.284709163297567 1105 86-87 2.3704106219328764 1105 88-89 2.161075940617952 1105 90-91 2.4097842252637953 1105 92-93 2.54737356455016 1105 94-95 2.377901932031559 1105 96-97 2.4259818189129554 1105 98-99 2.530895880973233 1105 100-101 2.2468281543166526 1106 1 0.07515163750229803 1106 2 0.10012332278082425 1106 3 0.01051217510151048 1106 4 0.16627654504836187 1106 5 0.10001549169012947 1106 6 0.0011033743600137313 1106 7 0.04911878760929511 1106 8 0.07830353892113351 1106 9 -0.13560296860426746 1106 10-11 0.09596719359466022 1106 12-13 0.14408061253089954 1106 14-15 0.16989069371595633 1106 16-17 0.030223766845224986 1106 18-19 0.18288424393005442 1106 20-21 0.179020056712055 1106 22-23 0.28991979613959984 1106 24-25 0.2312684857450833 1106 26-27 -0.16877818210451068 1106 28-29 0.210358977787088 1106 30-31 0.31149286515837815 1106 32-33 0.14763786929201217 1106 34-35 0.18554066439616435 1106 36-37 0.2903003867220022 1106 38-39 0.2566862884422605 1106 40-41 0.45830017571400816 1106 42-43 0.3888328223429909 1106 44-45 0.5195931085645498 1106 46-47 0.11794216546543623 1106 48-49 0.4559579767798496 1106 50-51 0.6093419601203323 1106 52-53 0.49447388998088115 1106 54-55 0.36879756378387896 1106 56-57 0.6942328160605769 1106 58-59 0.8274439929068436 1106 60-61 0.7894119407023048 1106 62-63 0.7606277220214999 1106 64-65 1.044236675185985 1106 66-67 1.0917857869523289 1106 68-69 0.9474921361994966 1106 70-71 0.6421571496652341 1106 72-73 0.9291290705585773 1106 74-75 0.7807896958610812 1106 76-77 0.8621097403794664 1106 78-79 1.3408930600283675 1106 80-81 1.24974872477803 1106 82-83 1.4566741575999522 1106 84-85 1.6608199298063688 1106 86-87 1.873829815955112 1106 88-89 1.534560434377962 1106 90-91 1.5927044101856502 1106 92-93 1.5136076737282345 1106 94-95 1.5063443795104234 1106 96-97 1.575307454272263 1106 98-99 1.5015153185016317 1106 100-101 1.4092338950263574 1107 1 0.014435423534564507 1107 2 0.0675209632453928 1107 3 0.07463197874143646 1107 4 0.06313147878193348 1107 5 0.03149829999683362 1107 6 0.03322524709269459 1107 7 0.060555958158907686 1107 8 0.036763219343640685 1107 9 0.23444374444720495 1107 10-11 0.15771908582979677 1107 12-13 0.09025590362440994 1107 14-15 0.19293113949903784 1107 16-17 0.10587836644521786 1107 18-19 0.09723223635545963 1107 20-21 0.30230934825152644 1107 22-23 0.28277984148306956 1107 24-25 0.3513466087112178 1107 26-27 0.3019554176754511 1107 28-29 0.268560700748246 1107 30-31 0.25268020520834256 1107 32-33 0.17621098761174636 1107 34-35 0.30142740307359617 1107 36-37 0.25776565754338066 1107 38-39 0.3284780344826217 1107 40-41 0.3067880854436069 1107 42-43 0.2971529408743905 1107 44-45 0.2511735988761501 1107 46-47 0.3924744407090941 1107 48-49 0.5309195774517406 1107 50-51 0.38725886589850234 1107 52-53 0.5123515078982237 1107 54-55 0.5728148635725603 1107 56-57 0.5966255158304676 1107 58-59 0.6628588697743183 1107 60-61 0.8061828543290055 1107 62-63 1.0618994557016386 1107 64-65 1.0598157098885608 1107 66-67 0.7519435269778398 1107 68-69 0.8920550516734345 1107 70-71 0.8201486469164436 1107 72-73 1.4108269943349185 1107 74-75 1.306891334949274 1107 76-77 1.3589263030821428 1107 78-79 1.4203358085260653 1107 80-81 1.483407362957319 1107 82-83 1.706302898068035 1107 84-85 1.6682836046645164 1107 86-87 1.9523987462862245 1107 88-89 2.049192361113061 1107 90-91 2.132130644425512 1107 92-93 2.1907547819220916 1107 94-95 2.1024349487259926 1107 96-97 2.097726776067123 1107 98-99 2.1080605787738094 1107 100-101 1.8166233179781202 1108 1 0.007709263136980837 1108 2 -0.03378600183842906 1108 3 -0.07146781307956473 1108 4 -0.12219034828722641 1108 5 -0.06627060332667156 1108 6 -0.11702422428309944 1108 7 -0.008005742465627463 1108 8 -0.06571465039485247 1108 9 -0.07270122695884851 1108 10-11 -0.10961390081109812 1108 12-13 -0.1881875945023097 1108 14-15 -0.033607022017115185 1108 16-17 -0.08211601779139954 1108 18-19 -0.2477807073740692 1108 20-21 -0.20235709927935375 1108 22-23 -0.07072624934708216 1108 24-25 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-0.2942628414510722 2316 58-59 -0.3143723719504621 2316 60-61 -0.31801822548166214 2316 62-63 -0.6875437363801282 2316 64-65 -0.5526221199346111 2316 66-67 -0.23157745953515985 2316 68-69 0.07611183285798617 2316 70-71 -0.31850385492473166 2316 72-73 -0.07791022164848371 2316 74-75 0.2113253312203618 2316 76-77 0.30812584498761453 2316 78-79 -0.0020029974500879177 2316 80-81 -0.26230633716209084 2316 82-83 -0.0030346011198965073 2316 84-85 0.2605803381538827 2316 86-87 0.6131470650827602 2316 88-89 0.6815723657009052 2316 90-91 0.8935373016290349 2316 92-93 0.7019547561657724 2316 94-95 1.0199240353482857 2316 96-97 0.9034598854124702 2316 98-99 0.6037343221040459 2316 100-101 0.18372833074417017 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 10.0 4 29.0 5 67.0 6 159.0 7 346.0 8 875.0 9 1652.0 10 2589.0 11 4030.0 12 6054.0 13 8264.0 14 11412.0 15 14930.0 16 18596.0 17 23185.0 18 23795.0 19 22681.0 20 23926.0 21 25950.0 22 28529.0 23 31475.0 24 35537.0 25 39788.0 26 45304.0 27 52919.0 28 61379.0 29 71981.0 30 83122.0 31 96051.0 32 111250.0 33 131831.0 34 158042.0 35 189586.0 36 213470.0 37 183577.0 38 58200.0 39 1149.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.66560451627246 19.071157339728693 14.794542388333861 23.46869575566499 2 16.312883969341392 20.321965923027914 38.84763591356989 24.51751419406081 3 19.613079352464403 23.582358286341933 29.694355251053906 27.11020711013976 4 13.614862741224329 16.67209206260832 35.56380714625671 34.14923804991065 5 14.173007382113767 36.9114668310005 33.98163255112 14.933893235765733 6 33.432356163399454 35.5570160471987 16.592619366194022 14.418008423207823 7 29.604365161325084 32.05432430248116 19.907124704783627 18.434185831410126 8 26.932324581588784 35.443723551135406 18.924478318946647 18.699473548329163 9 26.384716927760344 16.539094456193286 19.45944853783052 37.616740078215855 10-11 25.593968606039923 26.33675208110705 26.75165455867959 21.31762475417344 12-13 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28.308537124717407 22.76424406922733 38-39 22.5916135074581 26.168391645446487 28.40490940458412 22.835085442511293 40-41 22.87166490553752 25.9847518491856 28.51527691826256 22.628306327014318 42-43 22.801412170145174 26.024595109603148 28.193365157676432 22.980627562575247 44-45 22.691708557026875 26.310401465538213 28.248625145890145 22.749264831544767 46-47 23.024966577652656 26.575620937262524 27.69258961173952 22.7068228733453 48-49 22.95355000493898 26.54317904255606 27.635984743038282 22.86728620946668 50-51 23.03271401503246 26.682536885209096 27.539842985299796 22.744906114458644 52-53 23.35531928268034 26.524040490665325 27.372422749845093 22.748217476809238 54-55 22.86201047961997 26.677120843398377 27.53414631956106 22.926722357420594 56-57 22.854012697671493 26.880489004032004 27.397594716188184 22.86790358210832 58-59 22.90556331324814 26.53338526746828 27.383226771073733 23.177824648209842 60-61 22.61514561014377 26.594252597455075 27.733108684524826 23.05749310787633 62-63 22.734326592372415 26.68183532538906 27.740657468188473 22.843180614050056 64-65 22.536961019047652 26.60867847308982 27.57907710543951 23.27528340242302 66-67 22.437318733311535 26.814743515089944 27.505138958850445 23.242798792748072 68-69 22.762189103871 26.46733543822291 27.639935354764443 23.130540103141648 70-71 22.65074779324173 26.53441785218683 27.367170059646668 23.44766429492477 72-73 22.665755945667673 26.673667319867643 27.223523541754304 23.437053192710376 74-75 22.594828437755368 26.98431985739814 27.00267266229043 23.41817904255606 76-77 22.61430373835972 26.730439389721532 27.351263705672647 23.303993166246105 78-79 22.684740344291885 26.851893425767116 27.317785271060263 23.14558095888074 80-81 22.686406161164836 26.960877029826445 27.16174797571589 23.19096883329283 82-83 22.689120200435084 26.908151879864672 27.153840886691764 23.24888703300848 84-85 22.660677366095996 26.86802443728929 27.096636406778877 23.374661789835834 86-87 22.78765377011272 26.794158875554075 27.050434211578693 23.36775314275451 88-89 22.599599044531647 26.987631219748742 26.76307595254986 23.64969378316975 90-91 22.522908322091865 26.931369464868666 27.01336881212917 23.5323534009103 92-93 22.63382823525516 26.633275964110233 27.01214856206049 23.72074723857412 94-95 22.431028250972084 26.47173219048303 27.157128072270766 23.940111486274123 96-97 22.59895360949721 26.33316009482844 27.02130609111073 24.046580204563618 98-99 22.393200145475443 26.47947741089629 26.791531218850746 24.335791224777523 100-101 22.66628165052938 26.2277370452032 26.971755474342967 24.134225829924453 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 485.0 1 357.5 2 282.0 3 376.0 4 366.0 5 338.5 6 598.5 7 1292.5 8 1977.5 9 2243.0 10 1932.5 11 1549.0 12 1516.0 13 1604.0 14 1750.5 15 1853.0 16 1985.0 17 2135.5 18 2279.5 19 2461.0 20 2760.5 21 3212.5 22 3784.0 23 4623.5 24 5676.5 25 6965.0 26 8743.5 27 11045.5 28 13210.0 29 15461.5 30 18047.0 31 21352.5 32 25744.0 33 28064.0 34 29033.0 35 31276.0 36 34370.0 37 37797.5 38 41230.5 39 43936.0 40 46661.0 41 50972.5 42 54519.0 43 57403.5 44 60697.0 45 63861.5 46 65572.0 47 67575.5 48 69693.5 49 69688.5 50 68790.5 51 66995.5 52 65332.5 53 65474.0 54 66190.0 55 62773.5 56 57026.5 57 51969.5 58 47817.0 59 42873.0 60 35678.5 61 29501.0 62 24510.5 63 18969.5 64 14163.5 65 10757.0 66 7977.5 67 6284.0 68 4823.5 69 3465.5 70 2737.0 71 2051.0 72 1539.5 73 1203.5 74 882.5 75 704.0 76 497.0 77 269.0 78 157.5 79 95.5 80 56.0 81 28.0 82 13.5 83 8.0 84 4.0 85 4.5 86 3.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025368403092700186 2 0.01060758447902729 3 0.0019643674961161645 4 0.0 5 2.80623928016595E-4 6 0.0 7 0.0 8 2.2449914241327596E-4 9 4.489982848265519E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0016276187824962507 26-27 0.008587092197307807 28-29 2.2449914241327596E-4 30-31 0.0 32-33 3.6481110642157347E-4 34-35 0.009260589624547634 36-37 0.00112249571206638 38-39 0.012600014367945115 40-41 0.012151016083118563 42-43 0.00499510591869539 44-45 5.331854632315305E-4 46-47 1.1224957120663798E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 1.1224957120663798E-4 66-67 6.454350344381684E-4 68-69 0.0010944333192647205 70-71 8.418717840497849E-5 72-73 3.0868632081825447E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.6837435680995699E-4 82-83 0.00112249571206638 84-85 0.006875286236406577 86-87 0.0037322982426207132 88-89 0.0 90-91 0.0012628076760746775 92-93 5.893102488348494E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 2.8062392801659495E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1781744.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.81164932386172 #Duplication Level Percentage of deduplicated Percentage of total 1 78.7629866801311 44.74655178971601 2 12.100439126921444 13.748918086867935 3 3.724365930303942 6.347621135584967 4 1.7160065071200234 3.8995663967987046 5 0.9095282327658183 2.5835899505021676 6 0.5793672895639429 1.974888677065379 7 0.393705566497447 1.5656943806493695 8 0.27286260694816167 1.2401419791626942 9 0.21676235841213168 1.1083164383450927 >10 1.1923989507841233 12.450350176557551 >50 0.08469698633238154 3.2834904788399553 >100 0.04242035047265558 4.320750710488737 >500 0.003468432914247071 1.3851305843145354 >1k 7.927846661136162E-4 0.7384773350588855 >5k 1.9819616652840406E-4 0.6065118800479815 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5662 0.31777853608599216 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5111 0.28685377921856337 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3448 0.1935182607602439 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.6837435680995699E-4 2 0.0 0.0 0.0 0.0 2.24499142413276E-4 3 0.0 0.0 0.0 0.0 2.24499142413276E-4 4 0.0 0.0 0.0 0.0 2.24499142413276E-4 5 0.0 0.0 0.0 0.0 2.24499142413276E-4 6 0.0 0.0 0.0 0.0 2.24499142413276E-4 7 0.0 0.0 0.0 0.0 2.24499142413276E-4 8 5.6124785603319E-5 0.0 0.0 0.0 2.24499142413276E-4 9 5.6124785603319E-5 0.0 0.0 5.6124785603319E-5 2.24499142413276E-4 10-11 5.6124785603319E-5 0.0 0.0 5.6124785603319E-5 2.24499142413276E-4 12-13 5.6124785603319E-5 0.0 0.0 5.6124785603319E-5 2.8062392801659497E-4 14-15 2.24499142413276E-4 0.0 0.0 5.6124785603319E-5 3.0868632081825447E-4 16-17 2.24499142413276E-4 0.0 0.0 8.418717840497849E-5 3.3674871361991397E-4 18-19 2.24499142413276E-4 0.0 0.0 1.12249571206638E-4 3.3674871361991397E-4 20-21 2.24499142413276E-4 0.0 0.0 1.12249571206638E-4 3.92873499223233E-4 22-23 2.24499142413276E-4 0.0 0.0 2.8062392801659497E-4 5.051230704298709E-4 24-25 3.6481110642157347E-4 0.0 0.0 6.173726416365089E-4 5.051230704298709E-4 26-27 3.92873499223233E-4 0.0 0.0 0.0012347452832730179 5.051230704298709E-4 28-29 5.051230704298709E-4 0.0 0.0 0.003395549529000799 5.612478560331899E-4 30-31 6.173726416365089E-4 0.0 0.0 0.008951903303729379 5.893102488348494E-4 32-33 6.454350344381684E-4 0.0 0.0 0.02138354331486454 6.173726416365089E-4 34-35 7.85746998446466E-4 0.0 0.0 0.04257064988011746 6.173726416365089E-4 36-37 8.41871784049785E-4 0.0 0.0 0.08067937930477107 6.173726416365089E-4 38-39 8.699341768514445E-4 0.0 0.0 0.13728122558571826 6.454350344381684E-4 40-41 8.97996569653104E-4 0.0 0.0 0.19868174103574926 6.734974272398279E-4 42-43 9.821837480580825E-4 0.0 0.0 0.2616818128754748 6.734974272398279E-4 44-45 0.0010663709264630609 0.0 0.0 0.34283825285787406 7.296222128431469E-4 46-47 0.0012066828904713583 0.0 0.0 0.4441154284790632 7.85746998446466E-4 48-49 0.0012347452832730179 0.0 0.0 0.5486197792724432 8.41871784049785E-4 50-51 0.0012347452832730179 0.0 0.0 0.6634230282240322 8.97996569653104E-4 52-53 0.0012628076760746775 0.0 0.0 0.7744378541473971 0.0011224957120663799 54-55 0.0012908700688763369 0.0 0.0 0.8970985730834509 0.0011224957120663799 56-57 0.0013469948544796559 0.0 0.0 1.045520568611428 0.0011224957120663799 58-59 0.0013469948544796559 0.0 0.0 1.2072722007201933 0.0011224957120663799 60-61 0.0013469948544796559 0.0 0.0 1.3785650463815227 0.0011224957120663799 62-63 0.0013469948544796559 0.0 0.0 1.5469674655842813 0.0012347452832730179 64-65 0.0013469948544796559 0.0 0.0 1.7249952855180093 0.0012347452832730179 66-67 0.001571493996892932 0.0 0.0 1.935575481101662 0.0012347452832730179 68-69 0.001571493996892932 0.0 0.0 2.1712715182428 0.0012347452832730179 70-71 0.001571493996892932 0.0 0.0 2.421672249212008 0.0012347452832730179 72-73 0.001571493996892932 0.0 0.0 2.675664966459828 0.0012628076760746775 74-75 0.001571493996892932 0.0 0.0 2.9321271742741946 0.0012908700688763369 76-77 0.001571493996892932 0.0 0.0 3.2146593450013023 0.0012908700688763369 78-79 0.001571493996892932 0.0 0.0 3.5312592605896245 0.0013469948544796559 80-81 0.001627618782496251 0.0 0.0 3.8666329169622573 0.0013469948544796559 82-83 0.001627618782496251 0.0 0.0 4.210649790317801 0.0014031196400829749 84-85 0.0016556811752979103 0.0 0.0 4.563478255013066 0.0014031196400829749 86-87 0.00168374356809957 0.0 0.0 4.925651496511284 0.0014031196400829749 88-89 0.00168374356809957 0.0 0.0 5.321331235014682 0.0014031196400829749 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3240 0.0 60.42055 1 GTATCAA 5630 0.0 38.99115 1 ATCAACG 7105 0.0 30.215094 3 TATCAAC 7130 0.0 30.110842 2 TCAACGC 7185 0.0 29.87867 4 CAACGCA 7340 0.0 29.247717 5 AACGCAG 7540 0.0 28.471916 6 TGGTATC 1320 0.0 28.066914 2 GTGGTAT 1470 0.0 26.505072 1 GTACATG 6125 0.0 25.134563 1 TACATGG 6025 0.0 24.675234 2 ACGCAGA 8710 0.0 24.538269 7 CGCAGAG 8755 0.0 24.303648 8 ACATGGG 6125 0.0 23.57312 3 GAGTACT 6115 0.0 22.524292 12-13 GTAAGGT 880 0.0 21.588722 4 TAAGGTG 940 0.0 21.221254 5 CAGAGTA 9335 0.0 20.987429 10-11 GTACTTT 6515 0.0 20.849768 14-15 GCAGAGT 9920 0.0 20.731266 9 >>END_MODULE