FastQCFastQC Report
Thu 26 May 2016
SRR938503_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938503_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3329325
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83280.25014079430515196No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68690.20631809751225852No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA46560.13984816742132414No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46020.13822621702597374No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA45070.1353727857748943No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA44870.134772063406246No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA38490.11560901984636525No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA35270.10593738971112762No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG34320.10308395846004821No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65200.040.26441
GTATCAA107100.037.078891
ATCAACG134450.028.9707243
TATCAAC137100.028.6535782
TCAACGC136600.028.5843754
CAACGCA138150.028.1948155
AACGCAG141550.027.4952436
TACATGG109650.026.154412
GTACATG115050.025.9185451
ACATGGG114500.024.1742923
ACGCAGA161900.023.8942667
CGCAGAG161400.023.7614618
TAAGGTG19350.022.8585
TAAGGTA10000.021.877324
GTAAGGT18100.021.2834824
AGAGTAC141700.021.12652810-11
GCAGAGT176250.021.0025639
GAGTACT92700.020.86120812-13
AGGTAAG17400.020.2264062
CATGGGG81050.020.0095634
Produced by FastQC (version 0.11.5)