##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938503_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3329325 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.042459958099617 31.0 31.0 34.0 28.0 34.0 2 31.08308350791827 31.0 31.0 34.0 27.0 34.0 3 31.130744520285642 31.0 31.0 34.0 27.0 34.0 4 34.79206656003845 37.0 35.0 37.0 32.0 37.0 5 34.75932088336224 37.0 35.0 37.0 32.0 37.0 6 34.77444046465875 37.0 35.0 37.0 32.0 37.0 7 34.67526180231729 37.0 35.0 37.0 30.0 37.0 8 34.63535070922784 37.0 35.0 37.0 30.0 37.0 9 36.11554744580358 39.0 35.0 39.0 30.0 39.0 10-11 35.948244463967924 38.0 35.0 39.0 30.0 39.0 12-13 35.80711480555368 38.0 35.0 39.0 30.0 39.0 14-15 36.757508654156624 39.0 36.0 41.0 30.0 41.0 16-17 36.57021408243413 39.0 36.0 40.5 30.0 41.0 18-19 36.7053746630323 39.0 36.0 41.0 30.0 41.0 20-21 36.67282016624991 39.0 36.0 41.0 30.0 41.0 22-23 36.58422983637824 39.0 36.0 41.0 30.0 41.0 24-25 36.439602922514325 39.0 36.0 41.0 29.5 41.0 26-27 36.21253031770704 39.0 35.0 40.5 28.5 41.0 28-29 35.999375098556015 39.0 35.0 40.0 27.0 41.0 30-31 35.735333588640344 38.0 35.0 40.0 27.0 41.0 32-33 35.45676120534944 38.0 34.0 40.0 26.5 41.0 34-35 35.203419912444716 38.0 34.0 40.0 25.5 41.0 36-37 34.91350318758307 38.0 34.0 40.0 25.0 41.0 38-39 34.770864514578776 38.0 33.5 40.0 24.5 41.0 40-41 34.65910402258716 38.0 33.0 40.0 24.0 41.0 42-43 34.3531462083155 38.0 33.0 40.0 23.5 41.0 44-45 33.999121443535856 38.0 33.0 40.0 23.0 41.0 46-47 33.60392361815083 38.0 32.5 40.0 21.0 41.0 48-49 33.222911100598466 37.0 32.0 40.0 18.5 41.0 50-51 32.50004009821811 36.5 30.5 39.5 17.5 40.5 52-53 32.50161564281048 37.0 31.0 39.0 16.5 40.0 54-55 32.58475997386858 37.0 31.0 39.5 14.5 41.0 56-57 32.23323301269777 36.0 31.0 39.0 11.5 41.0 58-59 31.823542760169104 36.0 30.5 39.0 8.0 40.5 60-61 31.31008657911138 35.0 30.0 39.0 7.5 40.0 62-63 30.7167005323902 35.0 29.0 38.5 3.5 40.0 64-65 30.16891006435238 34.5 28.0 38.0 2.0 40.0 66-67 29.595767310190503 34.0 28.0 37.5 2.0 40.0 68-69 28.987337973913633 34.0 26.5 37.0 2.0 39.0 70-71 28.326360898981026 34.0 26.0 36.0 2.0 39.0 72-73 27.67578428059742 33.0 25.5 35.5 2.0 38.5 74-75 26.9952404766732 32.5 24.5 35.0 2.0 37.5 76-77 26.25306435989277 32.0 22.0 35.0 2.0 37.0 78-79 25.472347698106972 31.0 19.5 35.0 2.0 36.0 80-81 24.7803583308929 31.0 17.5 34.5 2.0 36.0 82-83 24.051633889752427 30.5 10.5 34.0 2.0 35.0 84-85 23.341703498456894 30.0 6.0 34.0 2.0 35.0 86-87 22.584413957784236 29.5 2.0 34.0 2.0 35.0 88-89 21.743423216417742 29.0 2.0 34.0 2.0 35.0 90-91 20.816374940866393 28.0 2.0 34.0 2.0 35.0 92-93 19.85901421459305 26.5 2.0 33.0 2.0 35.0 94-95 18.834617227215727 25.0 2.0 33.0 2.0 34.5 96-97 17.572625532202473 22.0 2.0 32.0 2.0 34.0 98-99 16.05020597268215 9.5 2.0 32.0 2.0 34.0 100-101 12.677891013944269 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3460578789001083 1101 2 0.35861359652396274 1101 3 0.32618437293544744 1101 4 0.22544339088172904 1101 5 0.24339175526378654 1101 6 0.38875084954017325 1101 7 0.2736877861686935 1101 8 0.3322943702936101 1101 9 0.5425077123787787 1101 10-11 0.5511439011573671 1101 12-13 0.5433166950247283 1101 14-15 0.6509495753166874 1101 16-17 0.6283038598017612 1101 18-19 0.651271654912172 1101 20-21 0.6848549513966375 1101 22-23 0.6579510756463733 1101 24-25 0.6413954085939935 1101 26-27 0.6527069131100234 1101 28-29 0.8442575643560701 1101 30-31 0.6781672243375496 1101 32-33 0.8248402676968354 1101 34-35 0.9207653554391442 1101 36-37 0.8416090717602458 1101 38-39 0.9011553142079762 1101 40-41 0.7639057536057905 1101 42-43 1.0423088054252574 1101 44-45 1.0281327785957615 1101 46-47 0.9255658482624014 1101 48-49 0.7601846364584404 1101 50-51 0.7764040108182328 1101 52-53 0.8112678737368952 1101 54-55 0.8032727870033867 1101 56-57 0.8842607739749511 1101 58-59 1.0428122168014653 1101 60-61 1.0345043622424157 1101 62-63 1.0471468860264963 1101 64-65 0.8013289959829955 1101 66-67 0.8081967205854035 1101 68-69 0.773114494367281 1101 70-71 0.9722603274449639 1101 72-73 1.096560897921023 1101 74-75 0.9647754894278933 1101 76-77 1.0870307532009171 1101 78-79 1.2442107142735495 1101 80-81 1.3584623371371016 1101 82-83 1.2909656612922937 1101 84-85 1.338647379205021 1101 86-87 1.4274658914452836 1101 88-89 1.2465240262956918 1101 90-91 0.8524364100129347 1101 92-93 0.8327960876664022 1101 94-95 0.7896766039207144 1101 96-97 0.6787298948896243 1101 98-99 0.6915746905768714 1101 100-101 0.7117830117853341 1102 1 0.5229056106956627 1102 2 0.41367673319655296 1102 3 0.37578503604966684 1102 4 0.27414971176546743 1102 5 0.33274419155205237 1102 6 0.44318144179759145 1102 7 0.39992975971528466 1102 8 0.5892204610217817 1102 9 0.7471054112240907 1102 10-11 0.6519593131506554 1102 12-13 0.7349526457181383 1102 14-15 0.7072868214181582 1102 16-17 0.8004086110011244 1102 18-19 0.6688945969001949 1102 20-21 0.7008402037203183 1102 22-23 0.7481795623958689 1102 24-25 0.6550927364421355 1102 26-27 0.570242255063377 1102 28-29 0.5241569145221732 1102 30-31 0.7354858614453832 1102 32-33 0.5827609796425648 1102 34-35 0.5561415369496174 1102 36-37 0.7618761015227093 1102 38-39 0.7427104052583857 1102 40-41 0.8830229076270015 1102 42-43 0.8853132560883452 1102 44-45 0.8439471908060412 1102 46-47 0.7452020375005262 1102 48-49 0.7153970139223773 1102 50-51 1.0079958006703578 1102 52-53 0.9613561002020248 1102 54-55 0.7223659362095987 1102 56-57 0.8147569119059241 1102 58-59 0.8042736640161792 1102 60-61 0.7626288344747536 1102 62-63 0.979962069497951 1102 64-65 1.0214568078413713 1102 66-67 0.6251995966529051 1102 68-69 0.69097838656511 1102 70-71 0.8920409966029865 1102 72-73 1.0076643025454857 1102 74-75 1.0462359543996556 1102 76-77 0.9168259405111421 1102 78-79 1.141969646795964 1102 80-81 1.4437828164516162 1102 82-83 1.26797917164318 1102 84-85 1.2445768691954129 1102 86-87 1.5579608767442146 1102 88-89 1.5177988323152398 1102 90-91 1.2622007568990519 1102 92-93 1.3868455095212084 1102 94-95 1.3496717528432818 1102 96-97 1.298568797022643 1102 98-99 1.5468984223183035 1102 100-101 1.2269367653816712 1103 1 0.4871610079929809 1103 2 0.49922924221365506 1103 3 0.42449457089034937 1103 4 0.3845693966223962 1103 5 0.3949828311433805 1103 6 0.5107813864175199 1103 7 0.40589444112047346 1103 8 0.48222206580692983 1103 9 0.650912030094517 1103 10-11 0.639707565990193 1103 12-13 0.6273066271601024 1103 14-15 0.6911563756513814 1103 16-17 0.6929838748533967 1103 18-19 0.8241038208226783 1103 20-21 0.7638942720861692 1103 22-23 0.8369467324788218 1103 24-25 0.8917712831814413 1103 26-27 0.8217249328517298 1103 28-29 0.9203771768733802 1103 30-31 0.9731346537648733 1103 32-33 1.0000545972793091 1103 34-35 0.9048246187651117 1103 36-37 0.888182350478985 1103 38-39 0.9280410133591985 1103 40-41 0.981870202781387 1103 42-43 0.977723426420134 1103 44-45 0.8452156038481462 1103 46-47 0.7277193473420525 1103 48-49 0.8281687818836687 1103 50-51 1.086204122049871 1103 52-53 1.1168270948881087 1103 54-55 1.1597810284217047 1103 56-57 1.1447619218043954 1103 58-59 1.0782264263352772 1103 60-61 1.3116038986923257 1103 62-63 1.208415771232481 1103 64-65 1.3971283507140662 1103 66-67 1.38304403668851 1103 68-69 1.4354059093201847 1103 70-71 1.4431949622492581 1103 72-73 1.4439372996684838 1103 74-75 1.6551583314228253 1103 76-77 1.6420015605277065 1103 78-79 1.5566491726778189 1103 80-81 1.7163327596204319 1103 82-83 1.5850986084792105 1103 84-85 1.7965138634180917 1103 86-87 1.9259186508648796 1103 88-89 1.7053507332933826 1103 90-91 1.6806048782423098 1103 92-93 1.689227376067599 1103 94-95 1.8162893672276361 1103 96-97 1.9910364408336108 1103 98-99 2.052713044656169 1103 100-101 1.7773426718283591 1104 1 0.43711652928077527 1104 2 0.46573745064008776 1104 3 0.47894312083003143 1104 4 0.2912918811317695 1104 5 0.37349839145298347 1104 6 0.6047057662720547 1104 7 0.3778634408607502 1104 8 0.5750377809549363 1104 9 0.6000327271297863 1104 10-11 0.7081241057364167 1104 12-13 0.7625921370544688 1104 14-15 0.843628405645326 1104 16-17 0.9147720638775212 1104 18-19 0.8047058987884768 1104 20-21 0.8690612233622943 1104 22-23 0.9409963314464491 1104 24-25 0.7670559152660275 1104 26-27 0.875047895360197 1104 28-29 0.9711215052719737 1104 30-31 0.9367241899102794 1104 32-33 0.9921861637715921 1104 34-35 0.820690623364122 1104 36-37 1.112955183261647 1104 38-39 1.108136043055417 1104 40-41 1.1023121302344734 1104 42-43 1.2282061765529377 1104 44-45 1.1785620590802992 1104 46-47 1.0559055932391956 1104 48-49 0.7604446259158735 1104 50-51 0.9239952358999872 1104 52-53 0.9380593226335918 1104 54-55 1.0774801170379575 1104 56-57 1.3111289566986883 1104 58-59 1.1698083210107768 1104 60-61 1.273406458458048 1104 62-63 1.189208795450078 1104 64-65 1.2436476539321895 1104 66-67 1.3645265940651434 1104 68-69 1.4740537926674087 1104 70-71 1.4792014978598296 1104 72-73 1.2803604329426292 1104 74-75 1.2780681713085116 1104 76-77 1.3556036755968073 1104 78-79 1.5041564191333272 1104 80-81 1.4026911431110918 1104 82-83 1.4887735998594778 1104 84-85 1.3810823294138217 1104 86-87 1.4951692908968077 1104 88-89 1.514597463904419 1104 90-91 1.4045208057840917 1104 92-93 1.457469349311591 1104 94-95 1.5907655093531865 1104 96-97 1.6364095688137503 1104 98-99 1.8101830249190236 1104 100-101 1.5869811137431444 1105 1 0.368440067804805 1105 2 0.27183964207949884 1105 3 0.3256957215722167 1105 4 0.28125948803586454 1105 5 0.2199234467036959 1105 6 0.23148187868745396 1105 7 0.271985006194285 1105 8 0.30743156533602445 1105 9 0.4290847901672308 1105 10-11 0.5151147836850214 1105 12-13 0.4855208378193723 1105 14-15 0.5354884784867622 1105 16-17 0.4983799869327328 1105 18-19 0.517660211097855 1105 20-21 0.559774456682085 1105 22-23 0.5136915506248059 1105 24-25 0.6401438275809141 1105 26-27 0.7337061636893196 1105 28-29 0.5574590888365236 1105 30-31 0.695742276342358 1105 32-33 0.698587242623482 1105 34-35 0.6677343660920201 1105 36-37 0.8783339002058881 1105 38-39 0.7630370048652892 1105 40-41 0.7291175031509525 1105 42-43 0.8325315670945699 1105 44-45 0.9167025198127092 1105 46-47 0.813870301632484 1105 48-49 0.7598477977541904 1105 50-51 0.8101280820172931 1105 52-53 0.80079228785835 1105 54-55 0.7837823986685635 1105 56-57 0.7997188243035325 1105 58-59 0.8908798405602312 1105 60-61 0.7848418065203191 1105 62-63 0.909320742684784 1105 64-65 1.0202436421453704 1105 66-67 1.2125207867162828 1105 68-69 1.1839418626447618 1105 70-71 1.2213871368059017 1105 72-73 1.1893051099411238 1105 74-75 1.2129425576985895 1105 76-77 1.3436881971359753 1105 78-79 1.2986856520951058 1105 80-81 1.365129609998533 1105 82-83 1.4616316451203097 1105 84-85 1.4364477586481605 1105 86-87 1.493985875748816 1105 88-89 1.4260625027692484 1105 90-91 1.428606070884232 1105 92-93 1.5820505799049975 1105 94-95 1.4364749732319595 1105 96-97 1.6138735611853683 1105 98-99 1.7402030941803517 1105 100-101 1.3354202381312543 1106 1 0.3485219369172228 1106 2 0.18339767178254363 1106 3 0.14494363192886794 1106 4 0.2333232618977661 1106 5 0.352684549983195 1106 6 0.19820602928597708 1106 7 0.42977676833371703 1106 8 0.2311363229181751 1106 9 0.21722391409974762 1106 10-11 0.184004675462333 1106 12-13 0.17699528865499303 1106 14-15 0.5108103278511678 1106 16-17 0.24873910414981992 1106 18-19 0.1683467052005696 1106 20-21 0.29344415055354034 1106 22-23 0.3074276760315584 1106 24-25 0.12353053639864697 1106 26-27 0.10591343509199191 1106 28-29 0.4173001193862049 1106 30-31 0.08048865238271219 1106 32-33 0.2630846175658874 1106 34-35 -0.05067185899550708 1106 36-37 0.07512056500944908 1106 38-39 0.1372843521700915 1106 40-41 0.37188067324274243 1106 42-43 -0.0783804121082241 1106 44-45 0.14415070530945684 1106 46-47 0.32814006114755045 1106 48-49 0.4994186784290875 1106 50-51 0.33003432530101406 1106 52-53 0.08497477090327976 1106 54-55 0.20843022518542398 1106 56-57 -0.3898012958737098 1106 58-59 -0.11739813745191086 1106 60-61 0.4936444355604692 1106 62-63 0.4204144091067761 1106 64-65 0.5844927936193649 1106 66-67 0.3524742336579152 1106 68-69 0.35341707943689116 1106 70-71 0.0939506071691163 1106 72-73 0.4463588364173994 1106 74-75 0.7661204017455887 1106 76-77 0.6895639539085643 1106 78-79 0.8623200814111591 1106 80-81 1.046912960005983 1106 82-83 0.8397108778454303 1106 84-85 1.0152048508698748 1106 86-87 0.8949662125362217 1106 88-89 0.8585680783558693 1106 90-91 0.7527136937823151 1106 92-93 0.7135324568452077 1106 94-95 0.7090528803819609 1106 96-97 0.674198258424834 1106 98-99 1.0213131680706091 1106 100-101 0.43152267477247186 1107 1 0.2186484912583424 1107 2 0.11264770898473841 1107 3 0.16270563151755368 1107 4 0.19170261727151683 1107 5 0.19267306328318767 1107 6 0.287391142389005 1107 7 0.2606183906503574 1107 8 0.26018170625125236 1107 9 0.3624662968459589 1107 10-11 0.309727234036977 1107 12-13 0.37436559511746736 1107 14-15 0.4654837594651724 1107 16-17 0.5466988354914619 1107 18-19 0.5232460251946165 1107 20-21 0.4704355129564206 1107 22-23 0.45704018800732626 1107 24-25 0.5319559974132559 1107 26-27 0.4888612032111297 1107 28-29 0.6057536868676436 1107 30-31 0.5629991484175463 1107 32-33 0.6421610166609995 1107 34-35 0.47752518890586515 1107 36-37 0.5806155799154027 1107 38-39 0.72430563516928 1107 40-41 0.5897025307441268 1107 42-43 0.7862402592701656 1107 44-45 0.6378711003047997 1107 46-47 0.5712742498805454 1107 48-49 0.6383751025374451 1107 50-51 0.608334034538693 1107 52-53 0.7191528866497592 1107 54-55 0.7651345622088783 1107 56-57 0.627548762562995 1107 58-59 0.5397390826329271 1107 60-61 0.43021126658559794 1107 62-63 0.5930361856829371 1107 64-65 0.5514214640547408 1107 66-67 0.5833672284429205 1107 68-69 0.8535626695974514 1107 70-71 0.7117762266901551 1107 72-73 0.9932751507082251 1107 74-75 1.0267135802843512 1107 76-77 1.0445020645052523 1107 78-79 1.136201382167073 1107 80-81 1.1301970526820817 1107 82-83 0.7990969078780523 1107 84-85 1.0994439113347276 1107 86-87 1.304894516801685 1107 88-89 1.2240619980961078 1107 90-91 1.1699301169209093 1107 92-93 1.3947050950910693 1107 94-95 1.411836273322404 1107 96-97 1.478435839161957 1107 98-99 1.4114852261294395 1107 100-101 1.3474804515358993 1108 1 0.21933021724209567 1108 2 0.20179302842629454 1108 3 0.21865515015370818 1108 4 0.1469689257599427 1108 5 0.14341217694046549 1108 6 0.16128271926375248 1108 7 0.32251050182413366 1108 8 0.3798503419087922 1108 9 0.32580249956296825 1108 10-11 0.35251098145205617 1108 12-13 0.3946548586753096 1108 14-15 0.5526778980069338 1108 16-17 0.4716436740638912 1108 18-19 0.5723347485167736 1108 20-21 0.4616841401047438 1108 22-23 0.3429099575661354 1108 24-25 0.4574319193264387 1108 26-27 0.5594806932523824 1108 28-29 0.5045003103946115 1108 30-31 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-2.1145835000124897 2316 78-79 -2.186974079190616 2316 80-81 -2.118012228491015 2316 82-83 -2.2222365470373227 2316 84-85 -2.065716907851229 2316 86-87 -2.1691197837091174 2316 88-89 -2.379806465522389 2316 90-91 -2.414717841501112 2316 92-93 -2.738226363416363 2316 94-95 -3.0833892459791272 2316 96-97 -2.954056769727078 2316 98-99 -2.9282765269297357 2316 100-101 -2.62623701180096 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11933.0 3 4307.0 4 4807.0 5 5502.0 6 6639.0 7 8171.0 8 10660.0 9 13140.0 10 16268.0 11 20328.0 12 24060.0 13 27380.0 14 31161.0 15 35421.0 16 40502.0 17 45847.0 18 45381.0 19 44870.0 20 47218.0 21 51701.0 22 56710.0 23 63263.0 24 69636.0 25 77367.0 26 87338.0 27 97792.0 28 109584.0 29 125403.0 30 143887.0 31 165404.0 32 193525.0 33 229959.0 34 275181.0 35 328652.0 36 367129.0 37 319138.0 38 119186.0 39 4875.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.288102744762554 17.512399732780953 14.03128289173303 24.16821463072346 2 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23.41413221145795 25.612960502314685 27.475163097425 23.497744188802365 46-47 23.981317456936758 25.979224863114748 26.675719753442824 23.363737926505678 48-49 23.62864065092962 26.004262210825484 26.55448361959596 23.812613518648938 50-51 23.728291954744265 26.19068610392226 26.683681599766178 23.397340341567297 52-53 24.06468958162798 25.799157398298274 26.615069202982898 23.521083817090847 54-55 23.52099710311757 26.087923017756342 26.727394005900106 23.663685873225983 56-57 23.48252363559569 26.264556752691526 26.597827551437454 23.655092060275333 58-59 23.61968986696052 25.714136360959866 26.59201847243135 24.074155299648265 60-61 23.15832341510818 26.044876194562157 26.92517594803736 23.8716244422923 62-63 23.346498760409155 25.99178532623634 27.17837100970555 23.48334490364896 64-65 23.070899101848884 26.1532610865317 26.86242182537698 23.91341798624244 66-67 22.94373848361978 26.16083009858352 26.845759959434705 24.049671458361995 68-69 23.50864729158672 25.656633169942616 27.070554900106863 23.7641646383638 70-71 23.39480117532479 25.811100446583158 26.57529591561265 24.218802462479395 72-73 23.279234357987036 25.93860490382004 26.64925861028788 24.132902127905048 74-75 23.245665955592944 26.432668329177055 26.446534266742784 23.875131448487217 76-77 23.5120837707157 25.863688721153043 26.619882025432368 24.004345482698884 78-79 23.450168856763266 26.17448041588957 26.54215651848984 23.83319420885733 80-81 23.670031622019618 26.089915872361484 26.261820710432865 23.978231795186034 82-83 23.50650349310061 26.138914804871177 26.386757036514602 23.967824665513614 84-85 23.34842127775635 26.287025989832834 26.352853643034198 24.01169908937662 86-87 23.715422915762012 26.23549855152615 26.05391924564906 23.99515928706278 88-89 23.476205857828592 26.233276492814806 25.89481749686375 24.395700152492843 90-91 23.354588136421555 26.140733608454408 26.049315369787152 24.45536288533689 92-93 23.53503464593544 25.762349538993107 26.276739338628808 24.425876476442642 94-95 23.33695143272529 25.539980298787697 26.478838025142785 24.64423024334423 96-97 23.638376125405685 25.214556341163252 26.40400101506811 24.74306651836295 98-99 23.36791466616353 25.561018419025043 25.950420821085956 25.120646093725473 100-101 24.02198793143613 24.757494007048763 26.228250659412506 24.992267402102605 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 407.0 1 306.5 2 292.5 3 395.0 4 352.0 5 357.0 6 632.5 7 1374.5 8 2224.0 9 2599.5 10 2341.0 11 1954.0 12 1919.0 13 2103.0 14 2327.0 15 2495.5 16 2714.5 17 2986.0 18 3258.0 19 3594.0 20 4104.5 21 4861.0 22 5814.5 23 7009.0 24 8659.5 25 11055.0 26 14085.5 27 17538.0 28 20767.5 29 23761.0 30 27441.0 31 34233.5 32 43904.5 33 46913.5 34 45892.5 35 48792.0 36 53985.0 37 60214.5 38 65977.5 39 70615.0 40 75946.5 41 82765.0 42 87662.0 43 92010.0 44 97218.5 45 104931.5 46 111260.5 47 117812.5 48 124483.5 49 126586.5 50 127981.5 51 128824.5 52 131427.0 53 137137.5 54 141638.5 55 137349.5 56 128670.0 57 120614.0 58 113804.5 59 102417.0 60 83756.0 61 68753.0 62 57958.0 63 45310.0 64 33218.0 65 24915.5 66 18660.5 67 14146.0 68 10671.5 69 8247.5 70 6499.0 71 4676.5 72 3337.0 73 2523.5 74 1850.0 75 1369.0 76 1015.5 77 645.5 78 411.0 79 270.0 80 160.5 81 104.5 82 63.0 83 44.0 84 34.5 85 19.5 86 13.0 87 11.0 88 9.0 89 12.5 90 8.5 91 1.5 92 4.5 93 4.5 94 2.0 95 0.5 96 0.0 97 1.0 98 2.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006637982173563711 2 0.10500627003972277 3 0.11548887537263561 4 0.11690057293895909 5 0.1578097662439083 6 0.09569507332567413 7 0.027392940010362462 8 0.026852289878578992 9 0.022647233298040893 10-11 0.05280349620418553 12-13 0.05484595225758975 14-15 0.02809878879352421 16-17 0.03179323136071126 18-19 0.03733489521149182 20-21 0.05820999752202023 22-23 0.03215366478190024 24-25 0.04942443288053884 26-27 0.0575492029165071 28-29 0.054155121533644206 30-31 0.06869260285493306 32-33 0.051046383275889264 34-35 0.0633611918331794 36-37 0.05435035630345491 38-39 0.03255915238073784 40-41 0.03831106906054531 42-43 0.033970849947061336 44-45 0.0077042643779144425 46-47 0.013275964347127422 48-49 0.009055889707373116 50-51 0.008665420167751722 52-53 0.010512641451345242 54-55 0.012059501550614613 56-57 0.015033077275423697 58-59 0.02069488559993392 60-61 0.02572593543736343 62-63 0.012134591846695651 64-65 0.009596539839156586 66-67 0.011143399938425957 68-69 0.011789176484722878 70-71 0.03164305076854918 72-73 0.029465432182199092 74-75 0.030787021393225356 76-77 0.024359292048688547 78-79 0.005166212370375376 80-81 0.004730688653105359 82-83 0.005736898620591261 84-85 0.005316392962537451 86-87 0.014312210433045737 88-89 0.039046953962139475 90-91 0.03647886583616799 92-93 0.030411569912820166 94-95 0.048388186794620536 96-97 0.04413807603643381 98-99 0.026777199582497954 100-101 0.07509029608103746 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3329325.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.38389023887417 #Duplication Level Percentage of deduplicated Percentage of total 1 73.43491594535641 34.06197080910362 2 13.293296280348022 12.331895911609939 3 4.894547688911725 6.810844883142502 4 2.50663141144189 4.650692650305395 5 1.4710605833728168 3.4116756316949473 6 0.8960627379493621 2.493772541051297 7 0.6180477620692012 2.006722169073975 8 0.44897074653809266 1.666000786231063 9 0.33130256437987793 1.3830391603848424 >10 1.8878007340725935 15.994160156911203 >50 0.14183221700972523 4.502380921368445 >100 0.0680525233890455 5.748311158282014 >500 0.0037068860377741826 1.1356170991884402 >1k 0.003641852946845932 3.344500496324041 >5k 1.30066176673069E-4 0.4584156253281784 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8328 0.25014079430515196 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6869 0.20631809751225852 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4656 0.13984816742132414 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4602 0.13822621702597374 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4507 0.1353727857748943 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4487 0.134772063406246 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3849 0.11560901984636525 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3527 0.10593738971112762 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3432 0.10308395846004821 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.003611843241498E-5 0.0 0.0 0.0 1.8021671059448988E-4 2 3.003611843241498E-5 0.0 0.0 0.0 1.8021671059448988E-4 3 3.003611843241498E-5 0.0 0.0 0.0 1.8021671059448988E-4 4 3.003611843241498E-5 0.0 0.0 0.0 1.8021671059448988E-4 5 3.003611843241498E-5 0.0 0.0 0.0 2.1025282902690484E-4 6 9.010835529724494E-5 0.0 0.0 0.0 2.703250658917348E-4 7 1.5018059216207488E-4 0.0 0.0 0.0 2.703250658917348E-4 8 1.5018059216207488E-4 0.0 0.0 0.0 2.703250658917348E-4 9 1.5018059216207488E-4 0.0 0.0 0.0 2.703250658917348E-4 10-11 1.5018059216207488E-4 0.0 0.0 0.0 2.703250658917348E-4 12-13 1.9523476981069736E-4 1.501805921620749E-5 0.0 0.0 6.607946055131296E-4 14-15 2.2527088824311233E-4 3.003611843241498E-5 0.0 3.003611843241498E-5 7.95957138458997E-4 16-17 2.853431251079423E-4 3.003611843241498E-5 0.0 4.505417764862247E-5 0.0010212280267021092 18-19 3.4541536197277224E-4 3.003611843241498E-5 0.0 6.007223686482996E-5 0.0011113363819993541 20-21 3.904695396213947E-4 3.003611843241498E-5 0.0 6.007223686482996E-5 0.0011563905596479766 22-23 4.505417764862247E-4 3.003611843241498E-5 0.0 1.651986513782824E-4 0.001201444737296599 24-25 5.406501317834696E-4 3.003611843241498E-5 0.0 3.4541536197277224E-4 0.0012615169741614291 26-27 5.406501317834696E-4 3.003611843241498E-5 0.0 7.95957138458997E-4 0.0013666433886748816 28-29 7.809390792427894E-4 3.003611843241498E-5 0.0 0.0024779797706742357 0.0014267156255397115 30-31 8.410113161076194E-4 3.003611843241498E-5 0.0 0.009311196714048644 0.001441733684755919 32-33 8.860654937562418E-4 3.003611843241498E-5 0.0 0.023773587739256453 0.001531842040053164 34-35 9.761738490534869E-4 3.003611843241498E-5 0.0 0.04639078491886493 0.0016519865137828238 36-37 0.0010813002635669392 3.003611843241498E-5 0.0 0.08572308200611234 0.0016519865137828238 38-39 0.0011714086188641842 3.003611843241498E-5 0.0 0.14587040916702335 0.001682022632215239 40-41 0.0011714086188641842 3.003611843241498E-5 0.0 0.21190481554068769 0.0017120587506476539 42-43 0.0013215892110262592 3.003611843241498E-5 0.0 0.27953113619127 0.0017120587506476539 44-45 0.0016519865137828238 3.003611843241498E-5 0.0 0.3646685138879503 0.0017120587506476539 46-47 0.0018772574020259362 3.003611843241498E-5 0.0 0.46892388096686266 0.0018171851651611064 48-49 0.0019523476981069736 3.003611843241498E-5 0.0 0.579261562028339 0.0019523476981069736 50-51 0.0019523476981069736 3.003611843241498E-5 0.0 0.6972884894085137 0.0021175463494852564 52-53 0.001967365757323181 3.003611843241498E-5 0.0 0.8160212655718502 0.0021926366455662935 54-55 0.0020424560534042188 3.003611843241498E-5 0.0 0.9470538322332605 0.0021926366455662935 56-57 0.0021926366455662935 3.003611843241498E-5 0.0 1.1001779640017122 0.0021926366455662935 58-59 0.0021926366455662935 3.003611843241498E-5 0.0 1.2684252814008845 0.0021926366455662935 60-61 0.002207654704782501 3.003611843241498E-5 0.0 1.450564303575049 0.0022527088824311234 62-63 0.0022226727639987082 3.003611843241498E-5 0.0 1.6307509780511065 0.002492997829890443 64-65 0.002312781119295953 3.003611843241498E-5 0.0 1.8192126031552942 0.002492997829890443 66-67 0.0025230339483228582 3.003611843241498E-5 0.0 2.0460754056753245 0.002583106185187688 68-69 0.0025230339483228582 3.003611843241498E-5 0.0 2.2985590172182047 0.002583106185187688 70-71 0.0025680881259714807 3.003611843241498E-5 0.0 2.5610596742582956 0.0026581964812687256 72-73 0.002583106185187688 3.003611843241498E-5 0.0 2.836250591336082 0.0028684493102956307 74-75 0.002583106185187688 3.003611843241498E-5 0.0 3.1101799914397064 0.002973575724809083 76-77 0.002583106185187688 3.003611843241498E-5 0.0 3.4113671690207474 0.002973575724809083 78-79 0.002598124244403896 3.003611843241498E-5 0.0 3.7519166798074686 0.0030186299024577055 80-81 0.002943539606376668 3.003611843241498E-5 0.0 4.108475441718666 0.0030486660208901206 82-83 0.002943539606376668 3.003611843241498E-5 0.0 4.474931104653345 0.0032288827314846105 84-85 0.003033647961673913 3.003611843241498E-5 0.0 4.853100853776667 0.003243900790700818 86-87 0.0031237563169711578 6.007223686482996E-5 0.0 5.249006930834328 0.0034090994420791 88-89 0.0031237563169711578 6.007223686482996E-5 0.0 5.673327175929055 0.0035442619750249674 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6520 0.0 40.2644 1 GTATCAA 10710 0.0 37.07889 1 ATCAACG 13445 0.0 28.970724 3 TATCAAC 13710 0.0 28.653578 2 TCAACGC 13660 0.0 28.584375 4 CAACGCA 13815 0.0 28.194815 5 AACGCAG 14155 0.0 27.495243 6 TACATGG 10965 0.0 26.15441 2 GTACATG 11505 0.0 25.918545 1 ACATGGG 11450 0.0 24.174292 3 ACGCAGA 16190 0.0 23.894266 7 CGCAGAG 16140 0.0 23.761461 8 TAAGGTG 1935 0.0 22.858 5 TAAGGTA 1000 0.0 21.87732 4 GTAAGGT 1810 0.0 21.283482 4 AGAGTAC 14170 0.0 21.126528 10-11 GCAGAGT 17625 0.0 21.002563 9 GAGTACT 9270 0.0 20.861208 12-13 AGGTAAG 1740 0.0 20.226406 2 CATGGGG 8105 0.0 20.009563 4 >>END_MODULE