FastQCFastQC Report
Thu 26 May 2016
SRR938503_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938503_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3329325
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84030.2523935031875831No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71090.21352676593603806No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49790.1495498336749942No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA46670.1401785647240807No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA45640.13708484452554195No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA41790.12552093892906221No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA38980.11708078964955358No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC37900.11383688885885276No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG36310.10906114602809878No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59600.041.9468771
GTATCAA99750.038.5951081
ATCAACG128050.029.6427523
TATCAAC130550.029.2347152
TCAACGC130700.029.0780774
CAACGCA131750.028.8459015
AACGCAG136500.027.8987186
TAAGGTG19200.025.7321975
GGACCGA11800.024.9489466
AGGTAAG17200.024.5935782
ACGCAGA155950.024.4192097
CGCAGAG156600.024.1965648
GTAAGGT18850.023.4381284
GTACATG107800.022.838691
TACATGG103150.022.7163142
AGAGTAC142300.021.50604610-11
ACATGGG106750.021.138643
AAGGTAA18400.020.9231641
GGTAAGG21350.020.9161283
AGTACTT100850.020.5989312-13
Produced by FastQC (version 0.11.5)