##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938503_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3329325 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.77837099111682 31.0 31.0 34.0 30.0 34.0 2 32.006005421519376 31.0 31.0 34.0 30.0 34.0 3 32.07051189054838 33.0 31.0 34.0 30.0 34.0 4 35.75162382765275 37.0 35.0 37.0 35.0 37.0 5 35.640912497277974 37.0 35.0 37.0 33.0 37.0 6 35.63693120977976 37.0 35.0 37.0 33.0 37.0 7 35.56986235948728 37.0 35.0 37.0 33.0 37.0 8 35.542442687331516 37.0 35.0 37.0 33.0 37.0 9 37.12890510839284 39.0 37.0 39.0 33.0 39.0 10-11 37.023221974424246 39.0 37.0 39.0 33.0 39.0 12-13 36.911993271909466 39.0 37.0 39.0 33.0 39.0 14-15 37.95382517477266 40.0 37.0 41.0 33.0 41.0 16-17 37.85566804081908 40.0 37.0 41.0 33.0 41.0 18-19 38.08272202323293 40.0 37.0 41.0 33.0 41.0 20-21 38.043058427759384 40.0 37.0 41.0 33.0 41.0 22-23 37.932722849226195 40.0 37.0 41.0 32.5 41.0 24-25 37.803952603005115 40.0 37.0 41.0 32.0 41.0 26-27 37.60252678846312 40.0 37.0 41.0 32.0 41.0 28-29 37.4886128569605 39.0 36.5 41.0 32.0 41.0 30-31 37.23224257169246 39.0 36.0 41.0 31.0 41.0 32-33 37.03463930376277 39.0 36.0 40.5 31.0 41.0 34-35 36.954712742072346 39.0 36.0 41.0 30.5 41.0 36-37 36.917583744452706 39.0 36.0 41.0 30.5 41.0 38-39 36.7476757300654 39.0 35.0 41.0 30.0 41.0 40-41 36.57249382382315 39.0 35.0 40.0 30.0 41.0 42-43 36.35373807002921 39.0 35.0 40.0 30.0 41.0 44-45 36.063043409700164 38.0 35.0 40.0 29.5 41.0 46-47 35.68340459402431 38.0 34.5 40.0 28.5 41.0 48-49 35.314357865333065 38.0 34.0 40.0 27.0 41.0 50-51 34.91837399472866 38.0 34.0 40.0 26.0 41.0 52-53 34.39513655170342 37.5 33.0 40.0 24.5 41.0 54-55 34.03403347525399 37.0 33.0 40.0 24.0 41.0 56-57 33.666031552942414 37.0 32.0 39.5 23.0 41.0 58-59 33.152398008605346 36.0 31.5 39.0 22.0 40.0 60-61 32.718207594632545 36.0 31.0 39.0 21.5 40.0 62-63 32.20752855308508 35.0 31.0 38.5 19.5 40.0 64-65 31.64414198073183 35.0 30.0 38.0 18.0 40.0 66-67 31.119759861233135 34.5 29.5 37.5 16.0 39.5 68-69 30.59145787809841 34.0 29.0 37.0 12.5 39.0 70-71 29.98766296471507 34.0 28.5 36.0 8.0 39.0 72-73 29.472598049154108 34.0 28.0 36.0 7.0 38.5 74-75 28.875044040458654 33.0 27.0 35.0 6.5 37.5 76-77 27.69619637614231 31.5 26.0 34.0 2.0 36.0 78-79 28.39187718231173 33.0 27.0 35.0 2.0 36.0 80-81 28.029704970226696 33.0 26.0 35.0 2.0 36.0 82-83 27.590861060425162 33.0 26.0 35.0 2.0 36.0 84-85 27.03549878729172 32.0 25.0 35.0 2.0 35.0 86-87 26.42162029840884 32.0 24.5 34.0 2.0 35.0 88-89 25.6507741058623 31.0 22.5 34.0 2.0 35.0 90-91 25.037965203156794 31.0 19.5 34.0 2.0 35.0 92-93 24.185916214247634 31.0 17.0 34.0 2.0 35.0 94-95 23.347580515569973 30.0 4.5 34.0 2.0 35.0 96-97 22.24387105494357 30.0 2.0 34.0 2.0 35.0 98-99 20.84852500131408 29.0 2.0 34.0 2.0 35.0 100-101 16.969534214893407 22.0 2.0 31.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24194577368401227 1101 2 0.2886291635104783 1101 3 0.2435667880762722 1101 4 0.19051450234516665 1101 5 0.03224029959277175 1101 6 0.1521182488893018 1101 7 0.19720783820631027 1101 8 0.26406581100525983 1101 9 0.3039725234117654 1101 10-11 0.2617485303664253 1101 12-13 0.32789992221612607 1101 14-15 0.3644691196040384 1101 16-17 0.4179118992439754 1101 18-19 0.39722251861994806 1101 20-21 0.36356373167667755 1101 22-23 0.40799855366990556 1101 24-25 0.3595165026941274 1101 26-27 0.3079882327900023 1101 28-29 0.30859336544530436 1101 30-31 0.3585611600136289 1101 32-33 0.20755098750560563 1101 34-35 0.28387392145631196 1101 36-37 0.29495909711305757 1101 38-39 0.3555834502439268 1101 40-41 0.48706493275670937 1101 42-43 0.5332168859263007 1101 44-45 0.4952583966006472 1101 46-47 0.35429536895304636 1101 48-49 0.37190227714661717 1101 50-51 0.4056730907838073 1101 52-53 0.4727044779948031 1101 54-55 0.4340948745274531 1101 56-57 0.3629982021141984 1101 58-59 0.41928721340858743 1101 60-61 0.38562834682461045 1101 62-63 0.44352778050907204 1101 64-65 0.40915266449163923 1101 66-67 0.27650126196042635 1101 68-69 0.34602931879824794 1101 70-71 0.41052599778127785 1101 72-73 0.5011883221763931 1101 74-75 0.24395956523126827 1101 76-77 0.44710121026606586 1101 78-79 0.6927313256684542 1101 80-81 0.6889091668330884 1101 82-83 0.453392996672239 1101 84-85 0.5665162264881474 1101 86-87 0.7154406571153018 1101 88-89 0.7358784035245023 1101 90-91 0.7794230560369648 1101 92-93 0.8994456517104865 1101 94-95 0.7293134628896034 1101 96-97 0.7764678557029931 1101 98-99 0.9617937419973366 1101 100-101 1.2244815155965583 1102 1 0.3140914182634127 1102 2 0.2713817791081894 1102 3 0.2905817467343681 1102 4 0.24596953390975074 1102 5 0.07260878102010793 1102 6 0.1887721480680753 1102 7 0.20592043341747512 1102 8 0.2514187313989851 1102 9 0.2501909131046247 1102 10-11 0.3868295528813377 1102 12-13 0.3443474301003775 1102 14-15 0.4510681685322666 1102 16-17 0.5464457563065821 1102 18-19 0.37950771687015816 1102 20-21 0.44078887529212096 1102 22-23 0.3777167551830658 1102 24-25 0.48723850588842055 1102 26-27 0.4565535817643891 1102 28-29 0.4497468895175203 1102 30-31 0.4092373290456166 1102 32-33 0.447903582656636 1102 34-35 0.5028689967106601 1102 36-37 0.6309695770439774 1102 38-39 0.6069567611994913 1102 40-41 0.4333513778540876 1102 42-43 0.4622344976426547 1102 44-45 0.5203195236843499 1102 46-47 0.5366422079599218 1102 48-49 0.5897329434731802 1102 50-51 0.7162356604115558 1102 52-53 0.6808709761934395 1102 54-55 0.6018184212463922 1102 56-57 0.7142821089153699 1102 58-59 0.9174591891151138 1102 60-61 0.7468761561973167 1102 62-63 0.8862516905576712 1102 64-65 0.9606472523830938 1102 66-67 0.767585552938872 1102 68-69 0.8596593429584161 1102 70-71 0.8179295036870116 1102 72-73 0.7073102014878039 1102 74-75 0.9122768677916646 1102 76-77 0.8678428237404354 1102 78-79 1.1481046205874001 1102 80-81 1.1707563147954616 1102 82-83 1.1493685568639371 1102 84-85 0.8783884366801225 1102 86-87 0.8728429853317827 1102 88-89 0.8358589090087776 1102 90-91 0.9874565004071805 1102 92-93 1.2175065437574766 1102 94-95 1.1129461882178902 1102 96-97 1.0601280437423846 1102 98-99 1.2129709050491648 1102 100-101 1.566487035200577 1103 1 0.2638428502237069 1103 2 0.24159690976282633 1103 3 0.2530519454508209 1103 4 0.19763405245755195 1103 5 0.14410911953319072 1103 6 0.1796086488657167 1103 7 0.2849644515368439 1103 8 0.2032286464305315 1103 9 0.293041864105156 1103 10-11 0.31026165210491996 1103 12-13 0.42881075007873903 1103 14-15 0.5175224497593263 1103 16-17 0.42016891017326685 1103 18-19 0.42552579913050437 1103 20-21 0.3794357935384767 1103 22-23 0.40640134995935995 1103 24-25 0.40201665968611877 1103 26-27 0.5258609359432782 1103 28-29 0.40410866508644006 1103 30-31 0.31156990542277896 1103 32-33 0.4291714152546575 1103 34-35 0.5289444953573224 1103 36-37 0.5255027929563241 1103 38-39 0.45074151350449654 1103 40-41 0.5711135470782764 1103 42-43 0.5829710526309313 1103 44-45 0.6649590988373433 1103 46-47 0.6750154610968195 1103 48-49 0.7579606240900034 1103 50-51 0.8240025255234826 1103 52-53 0.7229525057492268 1103 54-55 0.6770671439125877 1103 56-57 0.7669143514420398 1103 58-59 1.0009347074588177 1103 60-61 0.9660766277843393 1103 62-63 0.829532931413894 1103 64-65 0.8042249479154329 1103 66-67 0.959820590922277 1103 68-69 0.8316810344390682 1103 70-71 0.726001577713614 1103 72-73 0.8095505728958869 1103 74-75 0.7939047184500616 1103 76-77 0.7594517433579675 1103 78-79 1.005847911105846 1103 80-81 1.0487688076647999 1103 82-83 1.1795153473650188 1103 84-85 1.1727203051058197 1103 86-87 1.175730647041366 1103 88-89 1.1016070467110453 1103 90-91 1.1701242023577123 1103 92-93 1.4973278811672408 1103 94-95 1.368208270613355 1103 96-97 1.4205425664532108 1103 98-99 1.7162051679969927 1103 100-101 1.9132724930781784 1104 1 0.21465989204093816 1104 2 0.23842141024833552 1104 3 0.2444876893222201 1104 4 0.21277446129972333 1104 5 0.19332890952851756 1104 6 0.11722361253615077 1104 7 0.16618420895365915 1104 8 0.2129652763468357 1104 9 0.24706662196087592 1104 10-11 0.2728031105180051 1104 12-13 0.3041161057138666 1104 14-15 0.3695824521303166 1104 16-17 0.4301164859250832 1104 18-19 0.3714132150485412 1104 20-21 0.42987515090931794 1104 22-23 0.4314595161694683 1104 24-25 0.3974638585227055 1104 26-27 0.3251926240619838 1104 28-29 0.4360093133082401 1104 30-31 0.4051251526891235 1104 32-33 0.4594130934821692 1104 34-35 0.42488462511027336 1104 36-37 0.44706460660183467 1104 38-39 0.32807546944930976 1104 40-41 0.44196736641054457 1104 42-43 0.4878287795741656 1104 44-45 0.4845290534898723 1104 46-47 0.44023220319178336 1104 48-49 0.5393301973930065 1104 50-51 0.6537111831713389 1104 52-53 0.6427667992073722 1104 54-55 0.5757084391616516 1104 56-57 0.5472253494936581 1104 58-59 0.7354424959844863 1104 60-61 0.6588363259987702 1104 62-63 0.6041507447987726 1104 64-65 0.691959139873429 1104 66-67 0.6723268464481791 1104 68-69 0.801692608858783 1104 70-71 0.7112941462845832 1104 72-73 0.6914653837188283 1104 74-75 0.8387245547792617 1104 76-77 0.8188598733244952 1104 78-79 0.9914787531788178 1104 80-81 0.7758649405054747 1104 82-83 0.940522493822602 1104 84-85 0.962484364315447 1104 86-87 1.0327769257161066 1104 88-89 1.1281840500856362 1104 90-91 1.1843773446000014 1104 92-93 1.2745004377565579 1104 94-95 1.3152427667279163 1104 96-97 1.3474139980885695 1104 98-99 1.3923732206076629 1104 100-101 1.5317170865391283 1105 1 0.128994267277605 1105 2 0.20616773203122563 1105 3 0.1583717970834826 1105 4 0.10811223075451437 1105 5 0.07012498793288557 1105 6 0.10714061598545044 1105 7 0.09952517296668617 1105 8 0.10414684980445799 1105 9 0.17549233815466891 1105 10-11 0.1832290573533939 1105 12-13 0.2788947586292565 1105 14-15 0.34670908813657064 1105 16-17 0.2335929776942791 1105 18-19 0.21947789108315874 1105 20-21 0.22285426579637857 1105 22-23 0.1904707177777638 1105 24-25 0.1590203794835574 1105 26-27 0.07206665588363848 1105 28-29 0.1587932606531055 1105 30-31 0.26983371809838985 1105 32-33 0.30286166729092656 1105 34-35 0.34780996952358834 1105 36-37 0.40305595237833813 1105 38-39 0.33591306678350463 1105 40-41 0.3054981105911452 1105 42-43 0.4315920758901015 1105 44-45 0.4612327031349537 1105 46-47 0.34070760027398705 1105 48-49 0.41589072876370636 1105 50-51 0.4703207344873661 1105 52-53 0.3733736275755959 1105 54-55 0.3828997236632219 1105 56-57 0.30550503857502065 1105 58-59 0.5491318492929835 1105 60-61 0.4376047342963574 1105 62-63 0.5540111549965729 1105 64-65 0.4093432338650409 1105 66-67 0.39657063461591946 1105 68-69 0.5251350895984892 1105 70-71 0.605027407556765 1105 72-73 0.5887056881657386 1105 74-75 0.49675759504859585 1105 76-77 0.4586840177130185 1105 78-79 0.6063404198569557 1105 80-81 0.7842503116319008 1105 82-83 0.9421405995321877 1105 84-85 0.8999353960062884 1105 86-87 0.8947063459450924 1105 88-89 1.0156706246792595 1105 90-91 1.049492372793356 1105 92-93 1.3007306697062724 1105 94-95 1.2357124666184482 1105 96-97 1.1231128937628334 1105 98-99 1.3989994331972504 1105 100-101 1.4179152524220733 1106 1 -0.059660289425494284 1106 2 0.017877871565090686 1106 3 9.611249163015145E-4 1106 4 -0.0188442027652016 1106 5 -0.01811759677723046 1106 6 -0.09608822888620949 1106 7 -0.037625760635080496 1106 8 -0.0027291462651604093 1106 9 -0.26327285480388696 1106 10-11 0.05402475089938008 1106 12-13 0.006982342717954282 1106 14-15 0.0334099149988063 1106 16-17 0.11046297932163185 1106 18-19 0.010745300200063923 1106 20-21 0.11281020183917434 1106 22-23 0.14619887771744544 1106 24-25 0.17094077081832637 1106 26-27 0.03695492270704648 1106 28-29 0.06138752205611553 1106 30-31 0.048596655416297097 1106 32-33 -0.011742095268736819 1106 34-35 0.025969831960409806 1106 36-37 0.11769741194606809 1106 38-39 0.0938752293557954 1106 40-41 0.17937672759846635 1106 42-43 0.21092377282538166 1106 44-45 0.08247021213863803 1106 46-47 -0.5617424649232987 1106 48-49 -0.11997794866182687 1106 50-51 0.2808995775394152 1106 52-53 0.21249495082984282 1106 54-55 0.28921137183271384 1106 56-57 0.15672116190809504 1106 58-59 -0.01302310978618948 1106 60-61 0.2021761900394523 1106 62-63 -0.007382525364377557 1106 64-65 0.13669837847666955 1106 66-67 -0.0245329693279146 1106 68-69 -0.26123805996936866 1106 70-71 -0.0821213287694853 1106 72-73 0.03590108054764585 1106 74-75 0.09470827513970193 1106 76-77 0.04341812849278526 1106 78-79 0.4510608903623101 1106 80-81 -0.04995701092608584 1106 82-83 0.4183235920703794 1106 84-85 0.6197395088940674 1106 86-87 0.8728843530711323 1106 88-89 0.7035893944933633 1106 90-91 0.8469396622136784 1106 92-93 0.9200410565022956 1106 94-95 1.0797593348485215 1106 96-97 1.1689682742382566 1106 98-99 1.1929917643623753 1106 100-101 1.1154222545211567 1107 1 0.15148532079917487 1107 2 0.15088767120828095 1107 3 0.1989144190243124 1107 4 0.08334228305700009 1107 5 0.03619718896320734 1107 6 0.12002583889367457 1107 7 0.08791801840561675 1107 8 0.1259487295480639 1107 9 0.16347259691435312 1107 10-11 0.18481337053913194 1107 12-13 0.1413870574481848 1107 14-15 0.26130905274390415 1107 16-17 0.27622245584913685 1107 18-19 0.26088370072307043 1107 20-21 0.266625976572783 1107 22-23 0.2521824117754292 1107 24-25 0.2853748042217106 1107 26-27 0.28911657919953626 1107 28-29 0.1551714115831473 1107 30-31 0.17748344872090627 1107 32-33 0.19666434664651433 1107 34-35 0.34131197510776445 1107 36-37 0.279883262404951 1107 38-39 0.22135406590523843 1107 40-41 0.3700608801324847 1107 42-43 0.26149346800146844 1107 44-45 0.3134788263276391 1107 46-47 0.4173097736455986 1107 48-49 0.5570207705794843 1107 50-51 0.5099832916727323 1107 52-53 0.47360302445841995 1107 54-55 0.41277653753714816 1107 56-57 0.3907181155081787 1107 58-59 0.5021531700946937 1107 60-61 0.5385983505088134 1107 62-63 0.606346087553888 1107 64-65 0.47474510443276685 1107 66-67 0.5934290443056476 1107 68-69 0.6434137609317467 1107 70-71 0.7784268727343182 1107 72-73 0.769374904414903 1107 74-75 0.7627744131172065 1107 76-77 0.8213708123176069 1107 78-79 1.1280690564529685 1107 80-81 1.0941510810945871 1107 82-83 1.1512188173150335 1107 84-85 1.327866432749115 1107 86-87 1.4229498540133108 1107 88-89 1.410923911608883 1107 90-91 1.5174022067100132 1107 92-93 1.6645631378856471 1107 94-95 1.7488351153003983 1107 96-97 1.7671071195155825 1107 98-99 1.8266265190703344 1107 100-101 1.8899016328014326 1108 1 0.1640027597258289 1108 2 0.12201969254066825 1108 3 0.1505306735881149 1108 4 0.08937788320343998 1108 5 0.08546895917504571 1108 6 0.12183800310157977 1108 7 0.15523079112075067 1108 8 0.10365213422021213 1108 9 0.17631754135715028 1108 10-11 0.09748961501062325 1108 12-13 0.16648105449964845 1108 14-15 0.2717387676854841 1108 16-17 0.35138472444324265 1108 18-19 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-0.8632257066450961 2316 84-85 -0.9789513573202449 2316 86-87 -1.0168122837991653 2316 88-89 -1.1319572288614737 2316 90-91 -1.145544280261472 2316 92-93 -1.34619610350396 2316 94-95 -1.140847109250867 2316 96-97 -1.2586545381247696 2316 98-99 -1.33601420445056 2316 100-101 -1.5212273565132008 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 18.0 4 45.0 5 127.0 6 227.0 7 656.0 8 1509.0 9 2708.0 10 4525.0 11 6646.0 12 9540.0 13 13088.0 14 17380.0 15 22871.0 16 28467.0 17 35186.0 18 34322.0 19 31390.0 20 31944.0 21 34024.0 22 37467.0 23 41966.0 24 48156.0 25 56213.0 26 66898.0 27 79977.0 28 94771.0 29 111937.0 30 132451.0 31 157167.0 32 188564.0 33 231525.0 34 291185.0 35 374971.0 36 475087.0 37 476629.0 38 183883.0 39 5798.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.95445179598884 18.36597875594568 14.723900582343239 23.955668865722245 2 16.551895948160396 19.925508970447304 38.099631301258036 25.42296378013426 3 19.880816682060175 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22-23 23.61014620080647 25.017278277128245 28.00675812664729 23.36581739541799 24-25 23.556339810344042 25.357273897246834 27.84479805330726 23.24158823910186 26-27 23.053891855847848 25.57496260430246 27.782269933859176 23.58887560599052 28-29 23.252944104994334 26.03510173616404 27.624779515952568 23.087174642889057 30-31 23.21752778796936 25.551685675568557 27.44788014562336 23.782906390838722 32-33 22.95179314263007 25.488010498276143 27.585202753064188 23.974993606029603 34-35 23.798653810973 25.652209568594092 27.31339252912252 23.23574409131039 36-37 23.868619673491434 25.19722190427178 27.40097896690241 23.53317945533437 38-39 23.313969205630215 25.47773025189386 27.43079376903622 23.777506773439704 40-41 23.728632067877577 25.408871206095718 27.47778203627923 23.384714689747476 42-43 23.439001826220686 25.440275374855826 27.201091527297194 23.919631271626297 44-45 23.415058641955376 25.58287876286944 27.574913447751072 23.427149147424114 46-47 23.965578608276452 25.967215576731018 26.755934010647803 23.311271804344724 48-49 23.62217566623865 26.0332650011639 26.635459139615385 23.70910019298206 50-51 23.716429960820687 26.15306243820037 26.80693705719026 23.32357054378868 52-53 24.17075533328828 25.744407650199364 26.651137993437107 23.433699023075246 54-55 23.56821809413332 26.07885253199042 26.742675138573592 23.61025423530267 56-57 23.601060274980664 26.208135282677418 26.60660944786105 23.584194994480864 58-59 23.652843024183287 25.741245398620027 26.553834155345413 24.05207742185127 60-61 23.143865498261658 26.055656927455267 26.931555195122137 23.868922379160942 62-63 23.303789129723647 26.081150535757793 27.13378669958123 23.481273634937338 64-65 23.091129915787505 26.13043098183422 26.839101056493835 23.93933804588444 66-67 23.01672003914549 26.134917376038523 26.782506122490663 24.065856462325318 68-69 23.553106835213207 25.735581161275633 26.958809798145793 23.752502205365364 70-71 23.386649017419618 25.890388143766874 26.51483989000158 24.208122948811926 72-73 23.30939703197873 26.018176370718066 26.504146113811444 24.16828048349176 74-75 23.256397688580904 26.496869784319525 26.309133452171924 23.937599074927647 76-77 23.38758170386382 25.941132649721926 26.673736574512454 23.997549071901805 78-79 23.353950622161605 26.181653372063167 26.644795004620146 23.819601001155075 80-81 23.591629800434134 26.099760400784145 26.368216675804984 23.94039312297674 82-83 23.42727668248314 26.202069842460258 26.442081188014093 23.928572287042513 84-85 23.237508612328725 26.351825813689512 26.378727371561585 24.03193820242018 86-87 23.567941351625155 26.33676705489724 26.128836277041778 23.966455316435827 88-89 23.228503091970314 26.404583297573602 25.913331041704584 24.453582568751493 90-91 23.147137527906388 26.28019380274649 26.16629998927457 24.40636868007255 92-93 23.237206448930344 26.00573763005655 26.353982151998828 24.403073769014274 94-95 23.026312231672623 25.74778653383366 26.563561855375863 24.66233937911786 96-97 23.2284837384749 25.40181511073906 26.506193518165606 24.863507632620433 98-99 22.897081041608285 25.843145710514275 26.11308155849073 25.146691689386707 100-101 23.158065965320827 25.393739135815874 26.49245520760627 24.95573969125703 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 448.0 1 318.0 2 279.5 3 387.0 4 336.5 5 325.0 6 610.5 7 1408.0 8 2297.5 9 2613.5 10 2270.5 11 1860.5 12 1881.5 13 2105.0 14 2300.0 15 2441.5 16 2641.0 17 2903.0 18 3161.5 19 3525.5 20 4016.5 21 4667.5 22 5601.5 23 6882.5 24 8494.0 25 10662.5 26 13589.5 27 17087.0 28 20399.0 29 23777.0 30 27810.0 31 34615.5 32 45052.5 33 48018.0 34 46059.5 35 48902.5 36 54223.5 37 60291.0 38 66228.5 39 71367.0 40 76531.5 41 83114.5 42 87925.0 43 92493.5 44 98530.0 45 106199.0 46 111918.5 47 119158.5 48 126437.0 49 128541.0 50 129636.0 51 130460.5 52 133002.0 53 139524.5 54 145191.5 55 139264.0 56 129622.0 57 121474.0 58 113390.0 59 100994.5 60 81360.0 61 66460.5 62 56386.5 63 42790.0 64 29909.5 65 22075.5 66 16454.5 67 12607.0 68 9651.5 69 7469.0 70 5691.0 71 3900.0 72 2732.5 73 2059.5 74 1556.0 75 1122.5 76 791.5 77 520.0 78 300.0 79 185.0 80 112.5 81 60.5 82 40.5 83 18.5 84 10.5 85 9.5 86 4.0 87 3.0 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.106140133510547E-4 2 0.050340534492727504 3 0.0 4 6.007223686482996E-5 5 0.04598529732002733 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005751916679807468 26-27 0.0019523476981069736 28-29 7.509029608103744E-5 30-31 0.0015468600992693713 32-33 4.955959541348471E-4 34-35 0.011173436056858372 36-37 0.021445788560744295 38-39 0.0043552371727001715 40-41 0.0011113363819993543 42-43 0.001351625329458674 44-45 0.008004625562238592 46-47 0.0 48-49 0.0 50-51 5.106140133510547E-4 52-53 0.0 54-55 0.0014116975663235039 56-57 0.0 58-59 0.021145427376420146 60-61 0.0 62-63 0.009070907766589323 64-65 0.014537481321288851 66-67 0.006187440397077485 68-69 0.015363474578180263 70-71 1.351625329458674E-4 72-73 7.509029608103744E-5 74-75 0.021641023330554992 76-77 0.04823800620245845 78-79 0.029120016820226323 80-81 0.025530700667552735 82-83 0.034511500078844806 84-85 0.01566383576250441 86-87 0.009987009378777981 88-89 0.08183340466911461 90-91 0.023608389087878174 92-93 0.02321791954825678 94-95 0.025080158891066507 96-97 0.036298649125573505 98-99 0.050535769262538194 100-101 0.013200874051046382 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3329325.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.25572469740432 #Duplication Level Percentage of deduplicated Percentage of total 1 70.46287373725221 30.479226677665423 2 14.496133103483395 12.540814854024141 3 5.65026127028333 7.3321843792734525 4 2.846646972475585 4.925351110084136 5 1.5399141451314426 3.3305051159722203 6 1.0124356636100231 2.62761830033694 7 0.6978718460688282 2.113086671333579 8 0.5142398984623991 1.7795055581048553 9 0.37791512275814554 1.4712293258110891 >10 2.150425762669866 16.998791682222745 >50 0.1629689912911329 4.8585099446687625 >100 0.07927851764266618 6.189503855467528 >500 0.004934483000164261 1.3840231339921991 >1k 0.00396148634991211 3.503317950833996 >5k 1.3899952104954772E-4 0.4663314402091831 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8403 0.2523935031875831 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7109 0.21352676593603806 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4979 0.1495498336749942 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4667 0.1401785647240807 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4564 0.13708484452554195 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4179 0.12552093892906221 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3898 0.11708078964955358 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3790 0.11383688885885276 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3631 0.10906114602809878 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.5018059216207488E-4 2 0.0 0.0 0.0 0.0 1.5018059216207488E-4 3 0.0 0.0 0.0 0.0 1.5018059216207488E-4 4 0.0 0.0 0.0 0.0 1.5018059216207488E-4 5 0.0 0.0 0.0 0.0 1.5018059216207488E-4 6 0.0 0.0 0.0 0.0 1.5018059216207488E-4 7 0.0 0.0 0.0 0.0 1.8021671059448988E-4 8 0.0 0.0 0.0 0.0 1.8021671059448988E-4 9 0.0 0.0 0.0 0.0 1.8021671059448988E-4 10-11 6.007223686482996E-5 0.0 0.0 0.0 2.4028894745931984E-4 12-13 9.010835529724494E-5 0.0 0.0 1.501805921620749E-5 4.655598357024322E-4 14-15 1.2014447372965992E-4 0.0 0.0 6.007223686482996E-5 6.45776546296922E-4 16-17 1.2014447372965992E-4 0.0 0.0 6.007223686482996E-5 7.809390792427894E-4 18-19 2.703250658917348E-4 0.0 0.0 6.007223686482996E-5 7.95957138458997E-4 20-21 3.904695396213947E-4 0.0 0.0 6.007223686482996E-5 9.611557898372793E-4 22-23 3.904695396213947E-4 0.0 0.0 1.651986513782824E-4 9.911919082696943E-4 24-25 4.505417764862247E-4 0.0 0.0 3.4541536197277224E-4 0.0010212280267021094 26-27 4.8057789491863967E-4 0.0 0.0 8.109751976752045E-4 0.0011413725004317692 28-29 6.307584870807146E-4 0.0 0.0 0.0025380520075390656 0.001201444737296599 30-31 7.208668423779595E-4 0.0 0.0 0.009461377306210718 0.0012164627965128066 32-33 7.509029608103745E-4 0.0 0.0 0.024073948923580606 0.001351625329458674 34-35 8.560293753238268E-4 0.0 0.0 0.04688638087299978 0.001351625329458674 36-37 9.911919082696943E-4 0.0 0.0 0.08665420167751722 0.001351625329458674 38-39 0.001201444737296599 0.0 0.0 0.147582467917671 0.001411697566323504 40-41 0.001201444737296599 0.0 0.0 0.21390221741644327 0.001411697566323504 42-43 0.001351625329458674 0.0 0.0 0.2820992243172415 0.0014267156255397115 44-45 0.0016369684545666165 0.0 0.0 0.36803255915238076 0.0014567517439721266 46-47 0.001847221283593521 0.0 0.0 0.4726633777116983 0.0015168239808369564 48-49 0.0019223115796745587 0.0 0.0 0.58294098653631 0.0016369684545666163 50-51 0.0019223115796745587 0.0 0.0 0.7012082028639439 0.0016820226322152387 52-53 0.0019223115796745587 0.0 0.0 0.8202863943892531 0.0017871490467286913 54-55 0.0019823838165393885 0.0 0.0 0.9515442319389065 0.0018322032243773137 56-57 0.0021025282902690486 0.0 0.0 1.1050137790693308 0.0018772574020259362 58-59 0.0021025282902690486 0.0 0.0 1.273381240942233 0.0019223115796745587 60-61 0.0021175463494852564 0.0 0.0 1.455925750715235 0.0019223115796745587 62-63 0.002147582467917671 0.0 0.0 1.6360523529544277 0.0020424560534042188 64-65 0.0022226727639987082 0.0 0.0 1.824183580755859 0.0020724921718366335 66-67 0.0023428172377283683 0.0 0.0 2.0517221959406187 0.0021025282902690486 68-69 0.0023728533561607834 0.0 0.0 2.3047614756744985 0.0021325644087014637 70-71 0.0024479436522418206 0.0 0.0 2.5692895707087775 0.0021325644087014637 72-73 0.0025380520075390656 0.0 0.0 2.8484602734788584 0.0022677269416473307 74-75 0.0025530700667552733 0.0 0.0 3.126895091347345 0.0024479436522418206 76-77 0.0025530700667552733 0.0 0.0 3.4329481201144376 0.002508015889106651 78-79 0.0025680881259714807 0.0 0.0 3.7793546739954795 0.0025680881259714807 80-81 0.002883467369511838 0.0 0.0 4.145074452028565 0.0026281603628363105 82-83 0.002943539606376668 0.0 0.0 4.522057774473804 0.0026882325997011408 84-85 0.003003611843241498 0.0 0.0 4.9112808151802545 0.002703250658917348 86-87 0.0030636840801063275 0.0 0.0 5.3201024231638545 0.0028684493102956307 88-89 0.0031237563169711578 0.0 0.0 5.761242894580733 0.003063684080106328 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5960 0.0 41.946877 1 GTATCAA 9975 0.0 38.595108 1 ATCAACG 12805 0.0 29.642752 3 TATCAAC 13055 0.0 29.234715 2 TCAACGC 13070 0.0 29.078077 4 CAACGCA 13175 0.0 28.845901 5 AACGCAG 13650 0.0 27.898718 6 TAAGGTG 1920 0.0 25.732197 5 GGACCGA 1180 0.0 24.948946 6 AGGTAAG 1720 0.0 24.593578 2 ACGCAGA 15595 0.0 24.419209 7 CGCAGAG 15660 0.0 24.196564 8 GTAAGGT 1885 0.0 23.438128 4 GTACATG 10780 0.0 22.83869 1 TACATGG 10315 0.0 22.716314 2 AGAGTAC 14230 0.0 21.506046 10-11 ACATGGG 10675 0.0 21.13864 3 AAGGTAA 1840 0.0 20.923164 1 GGTAAGG 2135 0.0 20.916128 3 AGTACTT 10085 0.0 20.59893 12-13 >>END_MODULE