FastQCFastQC Report
Thu 26 May 2016
SRR938502_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938502_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3299941
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85070.257792487805085No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66630.20191270086343968No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA46910.1421540566937409No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46150.13985098521458414No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA44750.13560848512140067No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA43070.1305174850095805No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA39390.11936577047892674No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA35080.10630493090634044No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG33320.10097150221776692No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60600.043.531821
GTATCAA100350.038.7680851
TATCAAC129900.029.5611522
ATCAACG130650.029.2459553
TCAACGC132500.028.873484
CAACGCA133400.028.5687735
AACGCAG139550.027.2379196
TACATGG110850.026.6669832
GTACATG113100.026.4824241
TAAGGTG18500.025.6862875
GTGTAAG11950.024.7054881
ACATGGG115100.024.361043
ACGCAGA156700.024.0409777
CGCAGAG158800.023.633318
GTAAGGT18550.022.5453974
AGAGTAC138800.021.064310-11
GAGTACT91150.020.76604512-13
GCAGAGT174750.020.5778649
CATGGGG79650.020.3464074
AGTACTT95650.020.11185612-13
Produced by FastQC (version 0.11.5)