##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938502_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3299941 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.993434428070078 31.0 31.0 34.0 28.0 34.0 2 31.091439816651267 31.0 31.0 34.0 27.0 34.0 3 31.15704674719942 31.0 31.0 34.0 27.0 34.0 4 34.79440208173419 37.0 35.0 37.0 32.0 37.0 5 34.79947399059559 37.0 35.0 37.0 32.0 37.0 6 34.8254617279521 37.0 35.0 37.0 32.0 37.0 7 34.742778128457445 37.0 35.0 37.0 31.0 37.0 8 34.69681912494799 37.0 35.0 37.0 30.0 37.0 9 36.18759426304894 39.0 35.0 39.0 31.0 39.0 10-11 36.03826795691196 39.0 35.0 39.0 30.0 39.0 12-13 35.88867316112621 38.0 35.0 39.0 30.0 39.0 14-15 36.87158452226873 39.0 36.0 41.0 30.5 41.0 16-17 36.70945101745758 39.0 36.0 41.0 30.0 41.0 18-19 36.680862627543945 39.0 36.0 40.5 30.0 41.0 20-21 36.759793281152604 39.0 36.0 41.0 30.0 41.0 22-23 36.681563397648624 39.0 36.0 41.0 30.0 41.0 24-25 36.55484431388319 39.0 36.0 41.0 30.0 41.0 26-27 36.35765230348058 39.0 35.5 41.0 29.0 41.0 28-29 36.167347991979256 39.0 35.0 40.5 29.0 41.0 30-31 35.942959889282875 38.5 35.0 40.0 27.5 41.0 32-33 35.65690265371411 38.0 34.5 40.0 27.0 41.0 34-35 35.407488649039486 38.0 34.0 40.0 26.5 41.0 36-37 35.10638372019379 38.0 34.0 40.0 25.0 41.0 38-39 34.8397100129972 38.0 33.5 40.0 25.0 41.0 40-41 34.76785630409756 38.0 33.0 40.0 24.5 41.0 42-43 34.578050486357185 38.0 33.0 40.0 24.0 41.0 44-45 34.287907420162966 38.0 33.0 40.0 23.5 41.0 46-47 33.92443228530449 38.0 33.0 40.0 22.5 41.0 48-49 33.540415571066276 38.0 32.5 40.0 20.5 41.0 50-51 32.77483400460796 36.5 31.5 39.5 19.0 40.5 52-53 32.81922510129726 37.0 31.5 39.0 19.0 40.0 54-55 32.92705127152273 37.0 31.5 40.0 18.0 41.0 56-57 32.595369432362574 36.5 31.0 39.5 15.0 41.0 58-59 32.23337386941161 36.0 31.0 39.0 12.5 41.0 60-61 31.746684107382528 35.5 30.5 39.0 8.5 40.5 62-63 31.19838536507168 35.0 29.5 39.0 7.5 40.0 64-65 30.676447397089827 35.0 29.0 38.0 4.0 40.0 66-67 30.11562888548613 34.5 28.5 37.5 2.0 40.0 68-69 29.53336817234005 34.0 28.0 37.0 2.0 39.0 70-71 28.88548901328842 34.0 26.5 36.0 2.0 39.0 72-73 28.326820237089088 34.0 26.0 36.0 2.0 39.0 74-75 27.715522035090927 33.0 26.0 35.0 2.0 38.0 76-77 26.99102438498143 32.5 24.5 35.0 2.0 37.0 78-79 26.218197537471124 32.0 22.5 35.0 2.0 36.5 80-81 25.58211495296431 31.5 20.0 35.0 2.0 36.0 82-83 24.934223975519565 31.0 18.5 34.0 2.0 35.5 84-85 24.288801527057608 31.0 15.0 34.0 2.0 35.0 86-87 23.629431404985727 30.5 7.5 34.0 2.0 35.0 88-89 22.82853617685892 29.5 2.0 34.0 2.0 35.0 90-91 21.988816618236505 29.0 2.0 34.0 2.0 35.0 92-93 21.137412608286027 29.0 2.0 34.0 2.0 35.0 94-95 20.16722889894092 27.5 2.0 33.5 2.0 35.0 96-97 18.92778476948527 25.5 2.0 33.0 2.0 35.0 98-99 17.453027796557578 22.0 2.0 32.5 2.0 34.0 100-101 14.147721428958882 2.0 2.0 28.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5973425997026212 1101 2 0.5777676676043377 1101 3 0.5972798522049487 1101 4 0.5603513339890114 1101 5 0.49397570900652 1101 6 0.44848871827420567 1101 7 0.3855839516448185 1101 8 0.5909612628002492 1101 9 0.642804658070709 1101 10-11 0.5632965379536046 1101 12-13 0.6012239946834868 1101 14-15 0.8094261880561149 1101 16-17 0.8078217322076782 1101 18-19 0.7602986365336548 1101 20-21 0.7510868480913189 1101 22-23 0.9143212899588278 1101 24-25 0.8689353029721119 1101 26-27 0.9232244663886888 1101 28-29 0.921220910860221 1101 30-31 0.8506142813484274 1101 32-33 0.8903753503476821 1101 34-35 0.8626897499213655 1101 36-37 0.9034315719834893 1101 38-39 0.9263069290480885 1101 40-41 0.8560331092304168 1101 42-43 0.9950691014075659 1101 44-45 0.9064379480453084 1101 46-47 1.0846907149142595 1101 48-49 1.091254370027542 1101 50-51 0.9554511194623814 1101 52-53 1.0688420757443708 1101 54-55 1.1732593199501693 1101 56-57 0.9951973975440609 1101 58-59 1.0488995724458192 1101 60-61 1.0393871126144028 1101 62-63 0.8217375224708938 1101 64-65 0.7194469567784125 1101 66-67 0.616348790584162 1101 68-69 0.7949500764622144 1101 70-71 0.8688195751921057 1101 72-73 0.8549438646736434 1101 74-75 0.7152915114051481 1101 76-77 0.9846883314139561 1101 78-79 0.9858768853185005 1101 80-81 1.022963122387214 1101 82-83 1.049258112370648 1101 84-85 1.1357030401490036 1101 86-87 1.0949389025244294 1101 88-89 1.4097985213320463 1101 90-91 1.4961721635100638 1101 92-93 1.3743613285911174 1101 94-95 1.1656509522985488 1101 96-97 1.340110475560735 1101 98-99 1.3717143850090672 1101 100-101 1.0592271048923525 1102 1 0.6033790648800199 1102 2 0.7289001228860563 1102 3 0.6676168651310128 1102 4 0.6088580149136789 1102 5 0.6339864192696041 1102 6 0.6793393042378923 1102 7 0.511946618919346 1102 8 0.6500235868100148 1102 9 0.8048975339858586 1102 10-11 0.8402789841843941 1102 12-13 0.7496952261458532 1102 14-15 0.8243658736872632 1102 16-17 0.8128045605887024 1102 18-19 0.8787574564939646 1102 20-21 0.7525282919487495 1102 22-23 0.9165745935470184 1102 24-25 0.8376938929718492 1102 26-27 0.8406762450633565 1102 28-29 0.7178134884300178 1102 30-31 0.8287516844699923 1102 32-33 0.8712766382610084 1102 34-35 0.8359262439276378 1102 36-37 0.8560899883395194 1102 38-39 0.9866206693602422 1102 40-41 1.102358055930516 1102 42-43 0.9877854101715471 1102 44-45 1.067144900832993 1102 46-47 0.9052655241743679 1102 48-49 0.7456514442317328 1102 50-51 0.943145002041696 1102 52-53 0.8575492430725404 1102 54-55 0.8838530104487106 1102 56-57 0.9486641119236339 1102 58-59 0.9840218186176131 1102 60-61 0.9934010728305545 1102 62-63 0.9983604876885188 1102 64-65 1.0991607715093004 1102 66-67 1.079935781720959 1102 68-69 1.0450468710933372 1102 70-71 1.0888054875925448 1102 72-73 1.0784548196469608 1102 74-75 1.0908729077284605 1102 76-77 0.9993426614442633 1102 78-79 1.0210011847097427 1102 80-81 1.0462131089784314 1102 82-83 1.1040336074898534 1102 84-85 1.0729627296686992 1102 86-87 0.9383568257055295 1102 88-89 1.278650805182508 1102 90-91 1.2486140280679727 1102 92-93 1.3841491095050635 1102 94-95 1.2548149400026993 1102 96-97 1.2942633423579153 1102 98-99 1.1967851163239338 1102 100-101 0.9246551215326466 1103 1 0.5735552918259401 1103 2 0.6072961339162894 1103 3 0.5050751389219279 1103 4 0.5427205779914317 1103 5 0.5058388117583235 1103 6 0.538550330004874 1103 7 0.48350302047340676 1103 8 0.4379651310425885 1103 9 0.4661295596188708 1103 10-11 0.45884177445367413 1103 12-13 0.4631115281152134 1103 14-15 0.6324717131236923 1103 16-17 0.6617895251981807 1103 18-19 0.7399490816670067 1103 20-21 0.5931202179970754 1103 22-23 0.711643434814377 1103 24-25 0.7569749601580185 1103 26-27 0.6357635770421055 1103 28-29 0.5803423758055359 1103 30-31 0.7324150781644434 1103 32-33 0.80724644467373 1103 34-35 0.8653376392297432 1103 36-37 0.7794397097926478 1103 38-39 0.7942150487421884 1103 40-41 0.8912670481378981 1103 42-43 0.7895192806891131 1103 44-45 0.8399376681040138 1103 46-47 0.903282133717525 1103 48-49 0.8761526053088531 1103 50-51 0.9960692557147155 1103 52-53 1.0020932272417014 1103 54-55 0.9208923475183326 1103 56-57 0.9605843409014341 1103 58-59 0.8789712029118135 1103 60-61 0.9039154495330983 1103 62-63 1.1292007614347774 1103 64-65 0.9787817049483181 1103 66-67 0.9847633924698513 1103 68-69 0.9606406113246599 1103 70-71 0.948206653693557 1103 72-73 1.1133150438241586 1103 74-75 1.0431881541224222 1103 76-77 0.9681467380530222 1103 78-79 0.7985823484662724 1103 80-81 0.9442797481067302 1103 82-83 1.1744340255892602 1103 84-85 1.0877675096222568 1103 86-87 1.2637895083518877 1103 88-89 1.2852987392467234 1103 90-91 1.3170704793027355 1103 92-93 1.3710489638122105 1103 94-95 1.2268447735444283 1103 96-97 1.1667890758460615 1103 98-99 1.5690973344278056 1103 100-101 1.362260832580743 1104 1 0.5042920718943549 1104 2 0.5355310838248677 1104 3 0.5804055567741848 1104 4 0.497833876301236 1104 5 0.4356244521131174 1104 6 0.4836984449664641 1104 7 0.4124354283497951 1104 8 0.4086335724328052 1104 9 0.5293984162234722 1104 10-11 0.5481258323243736 1104 12-13 0.5464116139818671 1104 14-15 0.5629000425011768 1104 16-17 0.5860204314521482 1104 18-19 0.780804331396709 1104 20-21 0.7225547746369756 1104 22-23 0.7408643592016801 1104 24-25 0.7455590432634338 1104 26-27 0.7632415143092075 1104 28-29 0.7232346012941946 1104 30-31 0.7225401753924316 1104 32-33 0.7169827901179033 1104 34-35 0.6812282313740283 1104 36-37 0.7000793653117157 1104 38-39 0.6071408725238499 1104 40-41 0.6463510505437284 1104 42-43 0.7028130812353979 1104 44-45 0.7426240729455742 1104 46-47 0.8720390960230446 1104 48-49 0.7594292105990306 1104 50-51 0.6450797300728865 1104 52-53 0.6399604377654171 1104 54-55 0.973497085141716 1104 56-57 1.0998449896955975 1104 58-59 0.8186735973300117 1104 60-61 0.978898854399727 1104 62-63 0.9919115563318961 1104 64-65 0.9833625623582982 1104 66-67 0.988307596251893 1104 68-69 1.0315689215878265 1104 70-71 0.9581572510563916 1104 72-73 0.9453715314368907 1104 74-75 0.935866666325218 1104 76-77 1.0929867092967065 1104 78-79 1.1735590514062828 1104 80-81 0.9767129736086382 1104 82-83 1.0365631924025003 1104 84-85 0.9330030546962398 1104 86-87 0.977772920940513 1104 88-89 1.0726145280229709 1104 90-91 1.1904438678492646 1104 92-93 1.0148691153710878 1104 94-95 0.996920070595646 1104 96-97 0.8707070145202849 1104 98-99 0.6877344008868 1104 100-101 0.5368907322682492 1105 1 0.5070909938140318 1105 2 0.5321705744677026 1105 3 0.4661503330867234 1105 4 0.5245154263554141 1105 5 0.4379643238832216 1105 6 0.5015284590876519 1105 7 0.44056260846237194 1105 8 0.4265933517718352 1105 9 0.5636168211931292 1105 10-11 0.5854630190927708 1105 12-13 0.576993086914527 1105 14-15 0.7208420244267515 1105 16-17 0.639051287071176 1105 18-19 0.7234316681837143 1105 20-21 0.6944840082454249 1105 22-23 0.7110362075073269 1105 24-25 0.6492565415084144 1105 26-27 0.708303975089386 1105 28-29 0.7334162079442237 1105 30-31 0.8272681278493721 1105 32-33 0.6925436285596689 1105 34-35 0.8178637206949944 1105 36-37 0.851127581422432 1105 38-39 0.8205384528441897 1105 40-41 0.7945705215004963 1105 42-43 0.7752559028102226 1105 44-45 0.9143864148243779 1105 46-47 0.8861057822319012 1105 48-49 0.8623970003221828 1105 50-51 0.7621404777700533 1105 52-53 0.8254732940381047 1105 54-55 0.7750601595520052 1105 56-57 0.7570920674967283 1105 58-59 0.8389317235694378 1105 60-61 0.8453927102215566 1105 62-63 0.8873909095019137 1105 64-65 0.8706266389476447 1105 66-67 0.8934421646040605 1105 68-69 0.6531088458832528 1105 70-71 0.7088971632221899 1105 72-73 0.8942458568740328 1105 74-75 0.8662843254681434 1105 76-77 0.8463474690779762 1105 78-79 0.8408796437075559 1105 80-81 1.04275049296756 1105 82-83 1.107467135088708 1105 84-85 0.9095393177912605 1105 86-87 0.9565811681074621 1105 88-89 1.0808581252932932 1105 90-91 1.0165468198395047 1105 92-93 1.058317333266647 1105 94-95 1.254433362879464 1105 96-97 1.2789197049029326 1105 98-99 0.9078986226401646 1105 100-101 0.7036004718809092 1106 1 0.25304223853271424 1106 2 0.29254770018044596 1106 3 0.05345908375498709 1106 4 0.32089096432098785 1106 5 0.34102654819542266 1106 6 -0.030799092438137166 1106 7 0.23147338172027787 1106 8 0.34187559707519455 1106 9 0.24349355622708657 1106 10-11 0.130872096429286 1106 12-13 0.2384516418858027 1106 14-15 0.36080555809395776 1106 16-17 0.1435915198334783 1106 18-19 -0.1013646335097107 1106 20-21 0.2363056352213988 1106 22-23 0.14619984559394794 1106 24-25 0.29696177302805893 1106 26-27 0.06911133916087664 1106 28-29 0.21640393508771893 1106 30-31 0.2910657043893323 1106 32-33 0.36942867602221696 1106 34-35 0.3077386140852809 1106 36-37 0.14117854846416833 1106 38-39 0.2485556641665454 1106 40-41 0.32025014165702004 1106 42-43 -0.155032207144842 1106 44-45 0.15361704582871738 1106 46-47 -0.17306283186864846 1106 48-49 0.3151934826039664 1106 50-51 -0.06765883670937711 1106 52-53 0.19716204552884875 1106 54-55 0.005285018880826442 1106 56-57 -0.4273904753304336 1106 58-59 -0.8100667262314651 1106 60-61 -0.03422918960376009 1106 62-63 0.1460714433282604 1106 64-65 0.18308151420757568 1106 66-67 -0.5146541837562495 1106 68-69 -0.6414667088895527 1106 70-71 -0.5528504225259923 1106 72-73 -0.2462427240411742 1106 74-75 -0.22878006948038632 1106 76-77 -0.22257224598577352 1106 78-79 -0.02158099786388945 1106 80-81 -0.02389336175357215 1106 82-83 -0.19728028258713337 1106 84-85 -0.1390986704901671 1106 86-87 0.025939122845940688 1106 88-89 -0.10174357187969107 1106 90-91 0.0210116435512937 1106 92-93 0.1176364078675185 1106 94-95 -0.035068240065736944 1106 96-97 0.09901480881276115 1106 98-99 -0.10928386815608349 1106 100-101 -0.2992578306242635 1107 1 0.37378759446071896 1107 2 0.35536520130685645 1107 3 0.3372240065228951 1107 4 0.3577229866634397 1107 5 0.3750917320150364 1107 6 0.35514212105675114 1107 7 0.3876433507999195 1107 8 0.33543632169144644 1107 9 0.35990748311197507 1107 10-11 0.43663028138046656 1107 12-13 0.41613025937798653 1107 14-15 0.45893632126606576 1107 16-17 0.49279555289314914 1107 18-19 0.537364055006428 1107 20-21 0.5783629049438659 1107 22-23 0.5789476469470074 1107 24-25 0.49385039096736705 1107 26-27 0.4949736008523118 1107 28-29 0.5579803743974949 1107 30-31 0.608088236006175 1107 32-33 0.6268605669789622 1107 34-35 0.6654377476962949 1107 36-37 0.6163187046824561 1107 38-39 0.6864741981497033 1107 40-41 0.6211420683149029 1107 42-43 0.5474201827336458 1107 44-45 0.6676881282474483 1107 46-47 0.5660273393111055 1107 48-49 0.5976783405732675 1107 50-51 0.3375425927120759 1107 52-53 0.3818241400646514 1107 54-55 0.5307086689958709 1107 56-57 0.5248448013878146 1107 58-59 0.41863021647129983 1107 60-61 0.5027141838446347 1107 62-63 0.49058617606840826 1107 64-65 0.4410298335367493 1107 66-67 0.45984907853020474 1107 68-69 0.4995206443763429 1107 70-71 0.6683557292141771 1107 72-73 0.489013787633926 1107 74-75 0.6826097298984521 1107 76-77 0.6279709238491691 1107 78-79 0.6286378350045503 1107 80-81 0.5231268138630298 1107 82-83 0.578313962581344 1107 84-85 0.6057537269550366 1107 86-87 0.8197124893811072 1107 88-89 0.9452344708860103 1107 90-91 1.1046869695219819 1107 92-93 0.9730464378936254 1107 94-95 0.9481247937402877 1107 96-97 0.7567215091398509 1107 98-99 0.819198197007097 1107 100-101 0.5216557127656944 1108 1 0.34762506830565343 1108 2 0.2521699914243918 1108 3 0.30514138857156325 1108 4 0.2714395958617928 1108 5 0.3214582910471435 1108 6 0.28920249730273184 1108 7 0.2849542304709587 1108 8 0.2759105816307823 1108 9 0.1724791477047134 1108 10-11 0.27574078041444494 1108 12-13 0.4397988618050803 1108 14-15 0.560197396740385 1108 16-17 0.45830734937906925 1108 18-19 0.49549135477065676 1108 20-21 0.4735028793286631 1108 22-23 0.5289139349715697 1108 24-25 0.497841472379001 1108 26-27 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-1.1418144089187905 2316 46-47 -1.0671662049591362 2316 48-49 -1.2291806668723595 2316 50-51 -1.4033214736995454 2316 52-53 -1.226013512676058 2316 54-55 -1.0698538894472662 2316 56-57 -0.982474815239744 2316 58-59 -1.1296600937065975 2316 60-61 -1.2210266591535337 2316 62-63 -1.2850357171747255 2316 64-65 -1.158506163717341 2316 66-67 -1.2229430692350824 2316 68-69 -1.2136975218342059 2316 70-71 -1.472558415720119 2316 72-73 -1.2676542097152783 2316 74-75 -1.3129908088015192 2316 76-77 -1.2307433861048622 2316 78-79 -1.2279133205460333 2316 80-81 -1.160329205919929 2316 82-83 -1.098515913268887 2316 84-85 -1.2998358837572361 2316 86-87 -1.2940344613852055 2316 88-89 -1.3671154330573643 2316 90-91 -1.3828501344415791 2316 92-93 -1.430477474196568 2316 94-95 -1.2939273031280116 2316 96-97 -1.4293397746140215 2316 98-99 -1.2568536258527452 2316 100-101 -1.1111015045830204 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10756.0 3 3934.0 4 4250.0 5 4990.0 6 5800.0 7 7535.0 8 9307.0 9 12040.0 10 14892.0 11 18145.0 12 21812.0 13 25193.0 14 28474.0 15 32674.0 16 37318.0 17 42070.0 18 41542.0 19 40360.0 20 42672.0 21 46412.0 22 51038.0 23 56808.0 24 63236.0 25 71188.0 26 79716.0 27 89965.0 28 103033.0 29 118512.0 30 136479.0 31 158572.0 32 186853.0 33 223746.0 34 273040.0 35 333519.0 36 388584.0 37 358004.0 38 149966.0 39 7506.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.28079510567824 17.52540828261897 14.077853667599344 24.11594294410345 2 16.06811855109073 19.13054893455242 39.247022670813294 25.554309843543553 3 19.81862681343628 22.47977340023641 28.806855716062714 28.8947440702646 4 13.480963457405782 15.44379993499658 34.872242812028546 36.20299379556909 5 14.779979478820252 36.319759276373716 33.32008310350097 15.58017814130506 6 34.65697403104219 34.25195859545729 16.309232029240434 14.781835344260088 7 30.58715112419327 31.518759795198875 19.203379400450466 18.69070968015739 8 27.701057997365165 34.132040205233665 18.71520403628632 19.451697761114854 9 26.982333081007965 16.3031190629139 18.912246248542207 37.802301607535924 10-11 26.0857722320243 25.50942515354906 26.326312215411292 22.078490399015347 12-13 26.90753470752792 22.674801770875142 26.710624280779022 23.70703924081792 14-15 23.410729485715116 24.22133990042657 24.658656049704263 27.70927456415405 16-17 23.458046422296672 27.41844936482474 25.55652859774012 23.566975615138475 18-19 23.65361718575332 25.91632214571815 27.54489130064079 22.88516936788774 20-21 23.81243147134481 25.092982195775974 27.91958031092246 23.175006021956758 22-23 23.680946627880715 24.98209004546082 27.85526210077779 23.48170122588068 24-25 23.484936928533763 25.288884872703253 27.87780337422262 23.348374824540365 26-27 22.99907133443148 25.522992128323814 27.70361288198307 23.774323655261632 28-29 23.25529046432516 26.03434743184007 27.5718661257791 23.138495978055673 30-31 23.211160395053778 25.532720611587806 27.38167950156364 23.874439491794778 32-33 22.940139502640783 25.523604833371007 27.51585219383994 24.020403470148274 34-35 23.840670893860686 25.65015126248122 27.220319029907024 23.28885881375107 36-37 23.881165576004523 25.177242072424814 27.288412302123266 23.653180049447393 38-39 23.35203323546903 25.49823801819027 27.282377211419025 23.867351534921674 40-41 23.78895451285007 25.413583265804018 27.39145082671187 23.40601139463404 42-43 23.382537495675194 25.42849872873798 27.164316583570265 24.024647192016563 44-45 23.379786099384404 25.630904991766933 27.475681506489703 23.513627402358956 46-47 23.9271214017465 25.98813269428027 26.706529442704568 23.378216461268657 48-49 23.577917923518168 26.048655874791375 26.55487166400825 23.81855453768221 50-51 23.680602098671727 26.2146370601685 26.713097734204915 23.391663106954855 52-53 24.06284421248729 25.799133635779413 26.624432913597083 23.51358923813621 54-55 23.49820585400743 26.07121426103612 26.77937156399828 23.65120832095817 56-57 23.499858925414728 26.306424909377384 26.591468306101845 23.602247859106036 58-59 23.605638694532022 25.72777121306668 26.592974543501708 24.07361554889959 60-61 23.115848440807447 26.041089420153934 26.996066351508063 23.84699578753055 62-63 23.283617867263022 26.029244397748176 27.21156950934925 23.475568225639556 64-65 23.020508196202144 26.165796317546636 26.894795467325427 23.918900018925793 66-67 22.939962143366696 26.15468489474268 26.848344363234578 24.057008598656047 68-69 23.476657158685498 25.686414214209602 27.08879092664631 23.74813770045859 70-71 23.346391365422377 25.86899766355742 26.60430192166605 24.180309049354147 72-73 23.272029004659124 25.975121630681603 26.62716271051231 24.12568665414696 74-75 23.24236935521415 26.432003729244258 26.50045706277157 23.825169852770017 76-77 23.490531499236873 25.924362311233097 26.619103932267578 23.966002257262456 78-79 23.461520336762305 26.17479583080728 26.555685379202316 23.807998453228105 80-81 23.67525283932838 26.10424387009338 26.29733577717409 23.92316751340415 82-83 23.475221376652986 26.1492576936522 26.425066508836505 23.95045442085831 84-85 23.314731579710575 26.331631047356662 26.38252840304281 23.971108969889947 86-87 23.68114087670788 26.27791610651609 26.074216263984574 23.96672675279146 88-89 23.477577133378976 26.27120352336057 25.929464586264455 24.321754756996 90-91 23.335203712141123 26.190941728151202 26.078400701831605 24.395453857876067 92-93 23.52584944588737 25.768593909080245 26.357714612369705 24.347842032662683 94-95 23.34308143619186 25.578001368624548 26.560334289955918 24.518582905227674 96-97 23.59349519806222 25.24665983291927 26.459000774366746 24.70084419465176 98-99 23.32025220635093 25.58453239454336 26.059256823263844 25.03595857584187 100-101 23.921309016417318 24.835726716245475 26.271149673304144 24.971814594033066 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 406.0 1 302.0 2 290.5 3 415.5 4 376.0 5 368.5 6 666.0 7 1408.5 8 2314.5 9 2681.5 10 2352.5 11 2038.0 12 2046.5 13 2164.5 14 2347.5 15 2566.5 16 2760.0 17 2933.5 18 3213.0 19 3595.0 20 4068.5 21 4729.0 22 5772.0 23 7034.5 24 8515.0 25 10748.5 26 13742.0 27 17244.0 28 20450.5 29 23506.5 30 27433.5 31 33877.0 32 43183.5 33 46529.5 34 45667.5 35 48663.0 36 53842.0 37 59921.0 38 65858.0 39 70728.5 40 75378.5 41 81411.0 42 86681.0 43 91205.0 44 96770.0 45 104464.0 46 110579.0 47 117065.5 48 123581.0 49 125984.0 50 127202.5 51 128100.0 52 130601.0 53 135770.5 54 140465.0 55 136444.0 56 127885.5 57 119560.5 58 112298.0 59 100813.0 60 81773.5 61 67302.5 62 57473.0 63 44732.0 64 32389.5 65 24326.5 66 18215.0 67 13801.0 68 10580.0 69 8258.0 70 6404.0 71 4541.5 72 3203.5 73 2402.0 74 1807.0 75 1308.0 76 937.5 77 609.0 78 369.5 79 237.5 80 158.5 81 95.0 82 54.5 83 34.0 84 21.0 85 16.5 86 12.0 87 12.5 88 14.0 89 9.5 90 4.0 91 4.0 92 4.0 93 3.5 94 2.0 95 2.5 96 3.0 97 1.5 98 0.5 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1769122538857513 2 0.045909911722664135 3 0.043576536671413216 4 0.050091804671659274 5 0.057122233397506196 6 0.06187989421629054 7 0.03427334003850372 8 0.03233391142447699 9 0.03909160800147639 10-11 0.04003101873639559 12-13 0.037364304392108826 14-15 0.03239451856866532 16-17 0.030576304243015256 18-19 0.03874311692239346 20-21 0.048319045704150465 22-23 0.045334143852874945 24-25 0.06587996573272067 26-27 0.05030392967631846 28-29 0.042788643796964856 30-31 0.05171304577869726 32-33 0.04789479569483212 34-35 0.04653113495059457 36-37 0.04503110813193327 38-39 0.024697411256746713 40-41 0.022924652289237898 42-43 0.020015509368197796 44-45 0.004166741162948065 46-47 0.011257777032983318 48-49 0.010909285953900387 50-51 0.008560759116602387 52-53 0.008772884121261561 54-55 0.009727446642227846 56-57 0.008121357321236955 58-59 0.009454714493380336 60-61 0.010075937721310775 62-63 0.003924312586194723 64-65 0.006409205497916478 66-67 0.003727339367582633 68-69 0.00778801802820111 70-71 0.021045830819399498 72-73 0.018454875405348157 74-75 0.018576089693724828 76-77 0.011182018102747897 78-79 0.005015241181584761 80-81 0.006575875144434401 82-83 0.005803134056033123 84-85 0.004924330465302259 86-87 0.007060732297941084 88-89 0.02822777740571725 90-91 0.0346672864757279 92-93 0.021273107610105757 94-95 0.03056115245696817 96-97 0.03383393824313828 98-99 0.01995490222400946 100-101 0.1184566633161017 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3299941.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.28385878071252 #Duplication Level Percentage of deduplicated Percentage of total 1 73.25514394998191 33.9053073754173 2 13.325530168940263 12.33513913034711 3 5.050536085051855 7.012748967822982 4 2.5134941214585806 4.653368278549602 5 1.438253125864537 3.3283952284216296 6 0.9038524757114496 2.5100268206655674 7 0.6280180835306747 2.034697020290723 8 0.45649971382868876 1.6902854630626165 9 0.3332974027780038 1.3883660929939847 >10 1.8789695849767845 15.974790152974558 >50 0.14163583760863188 4.520336986687293 >100 0.06731828246304382 5.706949111941649 >500 0.0036926141346833746 1.14478129488256 >1k 0.0036266745946407935 3.331829343971003 >5k 1.3187907616875612E-4 0.4629787319713121 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8507 0.257792487805085 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6663 0.20191270086343968 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4691 0.1421540566937409 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4615 0.13985098521458414 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4475 0.13560848512140067 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4307 0.1305174850095805 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3939 0.11936577047892674 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3508 0.10630493090634044 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3332 0.10097150221776692 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.060714418833549E-5 2 0.0 0.0 0.0 0.0 6.060714418833549E-5 3 0.0 0.0 0.0 0.0 6.060714418833549E-5 4 0.0 0.0 0.0 0.0 6.060714418833549E-5 5 3.0303572094167745E-5 0.0 0.0 0.0 6.060714418833549E-5 6 6.060714418833549E-5 0.0 0.0 0.0 6.060714418833549E-5 7 2.1212500465917422E-4 0.0 0.0 0.0 9.091071628250324E-5 8 2.1212500465917422E-4 0.0 0.0 0.0 9.091071628250324E-5 9 2.1212500465917422E-4 0.0 0.0 0.0 9.091071628250324E-5 10-11 2.4242857675334196E-4 0.0 0.0 0.0 9.091071628250324E-5 12-13 3.6364286513001294E-4 0.0 0.0 0.0 5.303125116479355E-4 14-15 4.848571535066839E-4 1.5151786047083873E-5 0.0 0.0 5.757678697891871E-4 16-17 5.000089395537678E-4 3.0303572094167745E-5 0.0 0.0 5.757678697891871E-4 18-19 5.90919655836271E-4 3.0303572094167745E-5 0.0 0.0 6.060714418833549E-4 20-21 6.666785860716904E-4 3.0303572094167745E-5 0.0 4.545535814125162E-5 6.969821581658581E-4 22-23 6.666785860716904E-4 3.0303572094167745E-5 0.0 1.0606250232958711E-4 8.636518046837808E-4 24-25 8.788035907308646E-4 3.0303572094167745E-5 0.0 2.272767907062581E-4 0.0010151696651546195 26-27 8.788035907308646E-4 3.0303572094167745E-5 0.0 4.848571535066839E-4 0.0011060803814371227 28-29 0.001181839311672542 3.0303572094167745E-5 0.0 0.002590955414051342 0.0011363839535312903 30-31 0.001303053600049213 3.0303572094167745E-5 0.0 0.010227455581781612 0.0011666875256254581 32-33 0.0013182053860962969 3.0303572094167745E-5 0.0 0.026273197005643435 0.0012272946698137937 34-35 0.001424267888425884 3.0303572094167745E-5 0.0 0.049470581443728845 0.001303053600049213 36-37 0.0015757857488967227 3.0303572094167745E-5 0.0 0.09107738592902115 0.001303053600049213 38-39 0.0016363928930850581 3.0303572094167745E-5 0.0 0.1539876015965134 0.001303053600049213 40-41 0.0016363928930850581 3.0303572094167745E-5 0.0 0.22183729951535497 0.001303053600049213 42-43 0.0017273036093675615 3.0303572094167745E-5 0.0 0.29268705107151916 0.0013333571721433808 44-45 0.002015187544262155 3.0303572094167745E-5 0.0 0.3825522941167736 0.0014545714605200518 46-47 0.00215155361868591 3.0303572094167745E-5 0.0 0.48922086788824404 0.0016212411070379742 48-49 0.0021818571907800777 3.0303572094167745E-5 0.0 0.6040259507669985 0.0017121518233204776 50-51 0.0021818571907800777 3.0303572094167745E-5 0.0 0.7284372659996042 0.0018485178977442323 52-53 0.0021970089768271616 3.0303572094167745E-5 0.0 0.8523031169345149 0.002045491116356323 54-55 0.0022727679070625806 3.0303572094167745E-5 0.0 0.9896086020931889 0.0022273125489213294 56-57 0.0022727679070625806 3.0303572094167745E-5 0.0 1.14605079302933 0.0022727679070625806 58-59 0.0022727679070625806 3.0303572094167745E-5 0.0 1.3171750646450953 0.0023030714791567484 60-61 0.0023030714791567484 3.0303572094167745E-5 0.0 1.5048905419824172 0.0023030714791567484 62-63 0.0023030714791567484 3.0303572094167745E-5 0.0 1.6848028495054912 0.0024545893396275874 64-65 0.0023333750512509162 3.0303572094167745E-5 0.0 1.8761699072801605 0.0025606518419571743 66-67 0.002393982195439252 3.0303572094167745E-5 0.0 2.10567401053534 0.002606107200098426 68-69 0.002393982195439252 3.0303572094167745E-5 0.0 2.362163444740376 0.0026364107721925938 70-71 0.002484892911721755 3.0303572094167745E-5 0.0 2.628850030955099 0.0026818661303338455 72-73 0.0026364107721925938 3.0303572094167745E-5 0.0 2.9050519388073908 0.0028030804187105162 74-75 0.002727321488475097 3.0303572094167745E-5 0.0 3.182178105608555 0.0029242947070871874 76-77 0.002727321488475097 3.0303572094167745E-5 0.0 3.4873956837410125 0.0030000536373226065 78-79 0.002727321488475097 3.0303572094167745E-5 0.0 3.8340533967122443 0.0030152054233696904 80-81 0.0031212679256992777 3.0303572094167745E-5 0.0 4.194696208204935 0.003060660781510942 82-83 0.003197026855934697 3.0303572094167745E-5 0.0 4.562308841279283 0.003212178641981781 84-85 0.0032273304280288646 3.0303572094167745E-5 0.0 4.944679313963492 0.0032273304280288646 86-87 0.0032727857861701163 3.0303572094167745E-5 0.0 5.3496865550020445 0.0032879375722172 88-89 0.003303089358264284 3.0303572094167745E-5 0.0 5.773421403594791 0.0033485447164055358 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6060 0.0 43.53182 1 GTATCAA 10035 0.0 38.768085 1 TATCAAC 12990 0.0 29.561152 2 ATCAACG 13065 0.0 29.245955 3 TCAACGC 13250 0.0 28.87348 4 CAACGCA 13340 0.0 28.568773 5 AACGCAG 13955 0.0 27.237919 6 TACATGG 11085 0.0 26.666983 2 GTACATG 11310 0.0 26.482424 1 TAAGGTG 1850 0.0 25.686287 5 GTGTAAG 1195 0.0 24.705488 1 ACATGGG 11510 0.0 24.36104 3 ACGCAGA 15670 0.0 24.040977 7 CGCAGAG 15880 0.0 23.63331 8 GTAAGGT 1855 0.0 22.545397 4 AGAGTAC 13880 0.0 21.0643 10-11 GAGTACT 9115 0.0 20.766045 12-13 GCAGAGT 17475 0.0 20.577864 9 CATGGGG 7965 0.0 20.346407 4 AGTACTT 9565 0.0 20.111856 12-13 >>END_MODULE