FastQCFastQC Report
Thu 26 May 2016
SRR938502_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938502_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3299941
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83090.2517923805304398No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70360.21321593325456423No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50010.1515481640429329No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA47000.1424267888425884No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA44900.1360630387028132No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA40150.1216688419580835No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA38860.11775968115793585No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG36920.1118807881716673No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC36830.1116080560228198No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60700.041.6000251
GTATCAA100000.036.854411
ATCAACG127200.028.7753143
TCAACGC128100.028.498934
TATCAAC129250.028.4036712
CAACGCA130950.027.914985
AACGCAG137000.026.694676
TATAGCG1806.6556822E-926.4085145
TAAGGTG20400.025.1657625
AGGTAAG18350.025.1375772
GTAAGGT20300.024.8214024
ACGCAGA155650.023.4045687
CGCAGAG157300.023.0684838
GGTAAGG21250.022.593263
GTACATG109200.022.5577031
TACATGG107600.022.3185942
AAGGTAA20250.021.6048161
AGAGTAC139800.020.8260910-11
ACATGGG110600.020.7590983
GAGTACT91750.020.18891312-13
Produced by FastQC (version 0.11.5)