##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938502_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3299941 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.771302274798245 31.0 31.0 34.0 30.0 34.0 2 32.005790103520035 31.0 31.0 34.0 30.0 34.0 3 32.07025943797177 33.0 31.0 34.0 30.0 34.0 4 35.74768639802954 37.0 35.0 37.0 33.0 37.0 5 35.61120607913899 37.0 35.0 37.0 33.0 37.0 6 35.63680229434405 37.0 35.0 37.0 33.0 37.0 7 35.570171406094836 37.0 35.0 37.0 33.0 37.0 8 35.552805944106275 37.0 35.0 37.0 33.0 37.0 9 37.13192993450489 39.0 37.0 39.0 33.0 39.0 10-11 37.04020708249026 39.0 37.0 39.0 33.0 39.0 12-13 36.93002526408806 39.0 37.0 39.0 33.0 39.0 14-15 37.982989392840665 40.0 37.0 41.0 33.0 41.0 16-17 37.89349309578565 40.0 37.0 41.0 33.0 41.0 18-19 37.991579697940054 40.0 37.0 41.0 33.0 41.0 20-21 38.03162177748027 40.0 37.0 41.0 33.0 41.0 22-23 37.935730517606224 40.0 37.0 41.0 33.0 41.0 24-25 37.833274746427286 40.0 37.0 41.0 32.0 41.0 26-27 37.63697593381215 40.0 37.0 41.0 32.0 41.0 28-29 37.516725299028074 39.5 36.5 41.0 32.0 41.0 30-31 37.27478794317838 39.0 36.0 41.0 31.0 41.0 32-33 37.06434751409192 39.0 36.0 41.0 31.0 41.0 34-35 36.91476953678869 39.0 35.5 40.5 30.0 41.0 36-37 36.92631428864941 39.0 36.0 41.0 30.5 41.0 38-39 36.80523166929348 39.0 35.0 41.0 30.0 41.0 40-41 36.625157692213286 39.0 35.0 40.5 30.0 41.0 42-43 36.401953095525045 39.0 35.0 40.0 30.0 41.0 44-45 36.14389590601772 38.5 35.0 40.0 29.5 41.0 46-47 35.77981333605661 38.0 35.0 40.0 28.5 41.0 48-49 35.41453801749789 38.0 34.0 40.0 27.5 41.0 50-51 35.029330978947804 38.0 34.0 40.0 26.5 41.0 52-53 34.514293134331794 38.0 33.0 40.0 25.5 41.0 54-55 34.177382868360375 37.0 33.0 40.0 24.0 41.0 56-57 33.81252422391795 37.0 33.0 40.0 23.5 41.0 58-59 33.33445976761402 36.0 32.0 39.0 23.0 40.5 60-61 32.932177726813904 36.0 31.0 39.0 22.0 40.0 62-63 32.44775042947738 35.0 31.0 39.0 20.5 40.0 64-65 31.913037384607787 35.0 30.5 38.0 19.5 40.0 66-67 31.40206203686672 35.0 30.0 38.0 18.0 40.0 68-69 30.848373652741067 34.0 29.0 37.0 15.5 39.0 70-71 30.25602775928418 34.0 29.0 36.0 12.0 39.0 72-73 29.801897064220242 34.0 29.0 36.0 8.0 39.0 74-75 29.28315491095144 33.5 28.0 35.0 7.0 38.0 76-77 28.052427452490818 31.5 26.5 34.5 7.0 36.0 78-79 28.761630283692952 33.0 28.0 35.0 3.5 37.0 80-81 28.511921122226124 33.0 27.0 35.0 2.0 36.0 82-83 28.053054433397445 33.0 26.5 35.0 2.0 36.0 84-85 27.542596064596307 33.0 26.0 35.0 2.0 35.5 86-87 26.979770244377097 32.0 25.0 34.5 2.0 35.0 88-89 26.332503520517488 32.0 24.5 34.0 2.0 35.0 90-91 25.789985942172905 31.5 23.5 34.0 2.0 35.0 92-93 25.048792539018123 31.0 20.0 34.0 2.0 35.0 94-95 24.26583869226753 31.0 16.5 34.0 2.0 35.0 96-97 23.243800874015626 30.5 3.5 34.0 2.0 35.0 98-99 21.978903713733065 30.0 2.0 34.0 2.0 35.0 100-101 18.349462308568548 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24379999070815828 1101 2 0.2375960234057004 1101 3 0.26741419505040653 1101 4 0.17756850623665343 1101 5 0.1086522563173844 1101 6 0.17116256896465387 1101 7 0.31831658386138884 1101 8 0.27543846934435123 1101 9 0.345946813593919 1101 10-11 0.2805733857195065 1101 12-13 0.31204797765367687 1101 14-15 0.3776078670438352 1101 16-17 0.45397643674969146 1101 18-19 0.4500594497514996 1101 20-21 0.503371610155348 1101 22-23 0.40708618016409304 1101 24-25 0.41777126919578933 1101 26-27 0.5816165560611282 1101 28-29 0.483166040386088 1101 30-31 0.38715888042487734 1101 32-33 0.39845533507089925 1101 34-35 0.339241967252498 1101 36-37 0.40729870675526314 1101 38-39 0.47107700633748095 1101 40-41 0.43252019693889565 1101 42-43 0.47446005511142175 1101 44-45 0.42611577940803613 1101 46-47 0.5835468322590032 1101 48-49 0.5730272925189936 1101 50-51 0.6082668243116913 1101 52-53 0.6421979010761518 1101 54-55 0.45061436153066126 1101 56-57 0.6061868113501703 1101 58-59 0.6881279572415266 1101 60-61 0.5379292796058124 1101 62-63 0.5168166538375019 1101 64-65 0.43204124415413503 1101 66-67 0.32916542188401365 1101 68-69 0.3372633084605674 1101 70-71 0.211446115350153 1101 72-73 0.09933422722289009 1101 74-75 0.2237665929748509 1101 76-77 0.1547853859164796 1101 78-79 0.21995569097422774 1101 80-81 0.308222708304708 1101 82-83 0.42610115579691765 1101 84-85 0.46936262590221034 1101 86-87 0.38730629117296544 1101 88-89 0.5302752604236787 1101 90-91 0.44225476161037847 1101 92-93 0.3542209672661478 1101 94-95 0.5197262161777729 1101 96-97 0.5407544064635132 1101 98-99 0.3864682534115609 1101 100-101 0.29558657426910173 1102 1 0.38702587501720487 1102 2 0.30364222670962704 1102 3 0.3391105230559859 1102 4 0.23292204548415185 1102 5 0.23083766957598328 1102 6 0.24448932082177066 1102 7 0.3502152335972397 1102 8 0.302085486561225 1102 9 0.3883944020248222 1102 10-11 0.5066971671966201 1102 12-13 0.4831498776778105 1102 14-15 0.6231142496231499 1102 16-17 0.625004378971262 1102 18-19 0.5569374834879923 1102 20-21 0.47574826613968213 1102 22-23 0.5341953393665406 1102 24-25 0.4932589297659362 1102 26-27 0.5863446176208456 1102 28-29 0.48099779874646487 1102 30-31 0.5149939993607617 1102 32-33 0.5361890668684808 1102 34-35 0.5256411902136975 1102 36-37 0.5833218385769854 1102 38-39 0.5653809565640842 1102 40-41 0.6067147063786749 1102 42-43 0.5945222718509484 1102 44-45 0.6780638237374603 1102 46-47 0.5874982321356441 1102 48-49 0.8286019928327661 1102 50-51 0.805722005464311 1102 52-53 0.6180063667094515 1102 54-55 0.5872546492160353 1102 56-57 0.6999261262285472 1102 58-59 0.7547899168676935 1102 60-61 0.8281149744487948 1102 62-63 0.8021665978558445 1102 64-65 0.7438685005165979 1102 66-67 0.7646943312138426 1102 68-69 0.6297534278657544 1102 70-71 0.6855101825013215 1102 72-73 0.5886323446301702 1102 74-75 0.6207041952167991 1102 76-77 0.6713657614964106 1102 78-79 0.7048199019345667 1102 80-81 0.7444932907821133 1102 82-83 0.6222458382099632 1102 84-85 0.5287161718541 1102 86-87 0.5723011539341023 1102 88-89 0.6940301965308429 1102 90-91 0.57049256297271 1102 92-93 0.6465539525298958 1102 94-95 0.6336291935973861 1102 96-97 0.4103630293147589 1102 98-99 0.29132436882092705 1102 100-101 0.22583493839793434 1103 1 0.23001252263030025 1103 2 0.21491821139233735 1103 3 0.220056158968994 1103 4 0.22824075557212353 1103 5 0.19558460500223163 1103 6 0.2075009204287923 1103 7 0.29765556093167334 1103 8 0.17141337362414788 1103 9 0.3262732871675027 1103 10-11 0.34314473172571525 1103 12-13 0.28778330106905514 1103 14-15 0.48328539685665817 1103 16-17 0.354564467503387 1103 18-19 0.4364199110323099 1103 20-21 0.46285570484820227 1103 22-23 0.4571892018458854 1103 24-25 0.4455773160920202 1103 26-27 0.4064589530158358 1103 28-29 0.4746666489451812 1103 30-31 0.4588561996019038 1103 32-33 0.5149289353541633 1103 34-35 0.45729324107779945 1103 36-37 0.5276804969746962 1103 38-39 0.5538874094734751 1103 40-41 0.49473042278559376 1103 42-43 0.6222876449586181 1103 44-45 0.6714706640447048 1103 46-47 0.5217703819145925 1103 48-49 0.5937071596259571 1103 50-51 0.6388827588247423 1103 52-53 0.7324094446802434 1103 54-55 0.6141356081094429 1103 56-57 0.5771893195255586 1103 58-59 0.7518589646728131 1103 60-61 0.7116060486684148 1103 62-63 0.5918312670605061 1103 64-65 0.6920341521711322 1103 66-67 0.6028273415295651 1103 68-69 0.4068862790915837 1103 70-71 0.4834436885076627 1103 72-73 0.3814554366678564 1103 74-75 0.470907104608127 1103 76-77 0.3114813947300128 1103 78-79 0.46316619039524554 1103 80-81 0.4282072304061053 1103 82-83 0.48570030779867324 1103 84-85 0.2569969124873346 1103 86-87 0.48516908420249294 1103 88-89 0.5048189335245468 1103 90-91 0.5259760008774137 1103 92-93 0.5353839580003559 1103 94-95 0.5216934045115131 1103 96-97 0.4160138604896808 1103 98-99 0.2770708213953945 1103 100-101 0.17542716587973572 1104 1 0.2984784706940893 1104 2 0.26673869013488627 1104 3 0.21935250434071918 1104 4 0.18971545017448221 1104 5 0.157403738592798 1104 6 0.18697758099317952 1104 7 0.1282070227191099 1104 8 0.17350450819549934 1104 9 0.3172502822085548 1104 10-11 0.3115849016819965 1104 12-13 0.3589961333804581 1104 14-15 0.4295801800026169 1104 16-17 0.473062824101369 1104 18-19 0.3923978341424359 1104 20-21 0.35233040059456044 1104 22-23 0.3360440608999866 1104 24-25 0.4145023985624121 1104 26-27 0.4183638817537556 1104 28-29 0.5181402260513295 1104 30-31 0.5188611798102443 1104 32-33 0.406297568869455 1104 34-35 0.34635333257292444 1104 36-37 0.5144297489186087 1104 38-39 0.42308629853619095 1104 40-41 0.4799726469062122 1104 42-43 0.39941083294785074 1104 44-45 0.39391027782687615 1104 46-47 0.41509682646816515 1104 48-49 0.3900474310263391 1104 50-51 0.4737814639229043 1104 52-53 0.49914264441357403 1104 54-55 0.5446380114126939 1104 56-57 0.5516869157456341 1104 58-59 0.7008594957742744 1104 60-61 0.4538244016322963 1104 62-63 0.5178906648156598 1104 64-65 0.5744402220002556 1104 66-67 0.34846606039956995 1104 68-69 0.49403339477017383 1104 70-71 0.40775548501391157 1104 72-73 0.26879748021779903 1104 74-75 0.38260059375791755 1104 76-77 0.38887244051751324 1104 78-79 0.28651737106402564 1104 80-81 0.3099397232434775 1104 82-83 0.13163599923777625 1104 84-85 0.2675768522195696 1104 86-87 0.354088694545041 1104 88-89 0.32041156163406725 1104 90-91 0.336304279928477 1104 92-93 0.3346969104221138 1104 94-95 0.23164441180727025 1104 96-97 0.06648889171369632 1104 98-99 -0.02782241443491884 1104 100-101 0.007597243045321278 1105 1 0.22883671102673375 1105 2 0.2516750883314316 1105 3 0.21775661981956063 1105 4 0.149752134118593 1105 5 0.11365992919049006 1105 6 0.20296820730022347 1105 7 0.14640420410930233 1105 8 0.15707522992049405 1105 9 0.22188404788316518 1105 10-11 0.2262154154857825 1105 12-13 0.3069719826619419 1105 14-15 0.4007424328761431 1105 16-17 0.34431296217611873 1105 18-19 0.37062691865291697 1105 20-21 0.3934810000962088 1105 22-23 0.3192366877586963 1105 24-25 0.37557806935070204 1105 26-27 0.33705554602773447 1105 28-29 0.30839492193583595 1105 30-31 0.4679576086623456 1105 32-33 0.41818237815238035 1105 34-35 0.4800582679584977 1105 36-37 0.45386226604238544 1105 38-39 0.3394781588967959 1105 40-41 0.3379723902898064 1105 42-43 0.38433616695213857 1105 44-45 0.31926449816967306 1105 46-47 0.15329326842427093 1105 48-49 0.05175419787722291 1105 50-51 0.13220139163502864 1105 52-53 0.12192021016561227 1105 54-55 0.23261353833006382 1105 56-57 0.2911958987314591 1105 58-59 0.29857407075767384 1105 60-61 0.18902504279085264 1105 62-63 0.3019212744726829 1105 64-65 0.06707679716012649 1105 66-67 0.207083850473321 1105 68-69 0.12048850679580525 1105 70-71 0.10595323281789248 1105 72-73 0.3319973302183108 1105 74-75 0.2162655796690558 1105 76-77 0.2265112016521691 1105 78-79 0.3443631081764522 1105 80-81 0.45032251333407913 1105 82-83 0.36470698827912784 1105 84-85 0.4092018369323114 1105 86-87 0.507986008459671 1105 88-89 0.4098250328663937 1105 90-91 0.417902561235973 1105 92-93 0.4635792239328751 1105 94-95 0.4526033886053398 1105 96-97 0.26485360409796854 1105 98-99 0.059536714038202376 1105 100-101 -0.089697302417175 1106 1 0.05310188626002699 1106 2 0.16765259882254213 1106 3 0.14518218076098321 1106 4 0.09379557902930458 1106 5 0.07757748076992499 1106 6 -0.04058568223334191 1106 7 0.08037761292022338 1106 8 0.13204890156794846 1106 9 -0.011596050743108322 1106 10-11 0.04116553912622578 1106 12-13 0.04580339716944337 1106 14-15 0.10494701266848239 1106 16-17 -0.06251108754468504 1106 18-19 0.22925814828085578 1106 20-21 0.1548180602249758 1106 22-23 0.17936501277483075 1106 24-25 0.11029233744037725 1106 26-27 0.04164275858381217 1106 28-29 0.23011773489457 1106 30-31 0.22867709235843847 1106 32-33 0.1178648734186396 1106 34-35 0.13922308255146731 1106 36-37 -0.2669315449620626 1106 38-39 -0.0032063586015027568 1106 40-41 0.2337083593788023 1106 42-43 0.36414527075110925 1106 44-45 0.23938798663615302 1106 46-47 -0.03385778032922104 1106 48-49 0.00885373424789293 1106 50-51 -0.0011481827307235903 1106 52-53 -0.0071314953215946275 1106 54-55 -0.21879084907309476 1106 56-57 -0.018240156002903518 1106 58-59 -0.06857158716506007 1106 60-61 -0.2919437601228836 1106 62-63 -0.3401938979892023 1106 64-65 -0.6565895756779483 1106 66-67 -0.2175661478280766 1106 68-69 -0.8820620355950801 1106 70-71 -0.8718954144680033 1106 72-73 -0.46485506829907663 1106 74-75 -0.42782247593070366 1106 76-77 -0.380599822641436 1106 78-79 -0.4524144176914895 1106 80-81 -1.1324932972662793 1106 82-83 -0.7225434734727934 1106 84-85 -0.7068503227077976 1106 86-87 -0.5449359916726415 1106 88-89 -0.3189225332137511 1106 90-91 -0.4387999489422363 1106 92-93 -0.6604111351518789 1106 94-95 -0.46257291748656826 1106 96-97 -0.5856273241619618 1106 98-99 -0.8814173289060641 1106 100-101 -1.059976347194148 1107 1 0.16258882851566625 1107 2 0.20746263985478208 1107 3 0.18517691845389095 1107 4 0.16417618484810959 1107 5 0.11428362047213625 1107 6 0.11730571475441565 1107 7 0.17925034419518227 1107 8 0.16633541183288258 1107 9 0.24085556526668483 1107 10-11 0.09038530751593044 1107 12-13 0.1475906899016124 1107 14-15 0.23507268894373823 1107 16-17 0.21906180916619888 1107 18-19 0.2587127944164962 1107 20-21 0.27368558078409677 1107 22-23 0.22161703945094047 1107 24-25 0.2375101591203972 1107 26-27 0.1551603529184078 1107 28-29 0.22751203303006662 1107 30-31 0.18104366879774858 1107 32-33 0.25646312206780664 1107 34-35 0.29299483256092884 1107 36-37 0.28127712099149704 1107 38-39 0.2020034114047249 1107 40-41 0.15232454458449496 1107 42-43 0.18492011967474298 1107 44-45 0.29908911854809617 1107 46-47 0.2572960406904272 1107 48-49 0.27482982342508677 1107 50-51 0.25703539268020137 1107 52-53 0.37114953595302325 1107 54-55 0.29513689566717005 1107 56-57 0.3088480856642519 1107 58-59 0.2091919839121985 1107 60-61 0.05168257136119081 1107 62-63 0.17885668550778888 1107 64-65 0.25810928621916673 1107 66-67 0.13118628985830227 1107 68-69 0.20615134235895027 1107 70-71 0.1125562737695347 1107 72-73 0.11563931341423483 1107 74-75 0.12217202100305613 1107 76-77 0.15051270818433338 1107 78-79 0.26260972701163965 1107 80-81 0.4348282056211126 1107 82-83 0.47603828317473784 1107 84-85 0.5422253341347485 1107 86-87 0.555213364984521 1107 88-89 0.4499757701221405 1107 90-91 0.23920223164775933 1107 92-93 0.07141945983276443 1107 94-95 -0.01814717964576218 1107 96-97 0.2488231602798372 1107 98-99 0.024343177651179104 1107 100-101 -0.1927716124935337 1108 1 0.1638119534223037 1108 2 0.1379252137411484 1108 3 0.09200372465577544 1108 4 0.04621591677231862 1108 5 0.08655199534340596 1108 6 0.10167022844031237 1108 7 0.1322080371417229 1108 8 0.09035598138490286 1108 9 0.16126373924015525 1108 10-11 0.14010682298727772 1108 12-13 0.2535629565548092 1108 14-15 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-0.473432106999951 2316 62-63 -0.5141629410351811 2316 64-65 -0.3404069030058352 2316 66-67 -0.40407854966478496 2316 68-69 -0.361651111456311 2316 70-71 -0.4097410833105535 2316 72-73 -0.37370759697869005 2316 74-75 -0.11491407701465306 2316 76-77 -0.17709448468316324 2316 78-79 -0.07392716521811948 2316 80-81 -0.20277454552729068 2316 82-83 0.03503902416218452 2316 84-85 0.10085586446727746 2316 86-87 0.18261334502428284 2316 88-89 0.3458339155895729 2316 90-91 0.21797607940096242 2316 92-93 0.1156902245056024 2316 94-95 0.15230144530572787 2316 96-97 0.28700613322627433 2316 98-99 0.2884823535650938 2316 100-101 0.4826411915947908 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 19.0 4 36.0 5 91.0 6 243.0 7 652.0 8 1490.0 9 2614.0 10 4209.0 11 6392.0 12 9153.0 13 12359.0 14 16287.0 15 21739.0 16 26959.0 17 33107.0 18 31996.0 19 28977.0 20 29102.0 21 30700.0 22 33408.0 23 37690.0 24 44101.0 25 51617.0 26 61279.0 27 74041.0 28 89579.0 29 106795.0 30 126901.0 31 150510.0 32 181109.0 33 224221.0 34 284430.0 35 369914.0 36 482471.0 37 504838.0 38 212920.0 39 7988.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.9756651750148 18.35713642841753 14.723928239494219 23.943270157073457 2 16.544098141326153 19.925558455591105 38.1029199676088 25.42742343547394 3 19.880336094116537 22.715232450349387 29.308293046868865 28.09613840866521 4 14.004159468305646 16.167289051531526 34.53143556202974 35.29711591813308 5 14.659298251573485 36.5346773684798 33.39947154136196 15.406552838584759 6 34.73568163794444 34.67098351152339 15.942012296583485 14.651322553948692 7 30.50806059865919 31.597746747593362 19.21619204706993 18.678000606677514 8 27.634251642680884 34.56301188415187 18.562422782710357 19.24031369045689 9 26.76099360564325 16.28729119702443 18.824275949176062 38.12743924815625 10-11 26.055753724081733 25.719778020273697 26.287106345234655 21.937361910409912 12-13 26.82028254444549 22.706466570159893 26.81247937463124 23.66077151076337 14-15 23.20324817928563 24.356647588547794 24.69180509590929 27.748299136257288 16-17 23.30959856555011 27.513325238239105 25.666186153025162 23.51089004318562 18-19 23.490238158803443 26.01407116066621 27.676570581110386 22.81912009941996 20-21 23.712469404756025 25.18116232987196 27.987303409363985 23.11906485600803 22-23 23.61808892946874 25.034068790926867 28.037470972965878 23.310371306638515 24-25 23.55004763728751 25.341552078585767 27.897238007040137 23.211162277086586 26-27 23.03890289452155 25.61237665223237 27.79776550009857 23.55095495314751 28-29 23.23530935856126 26.03861705406248 27.665600688012297 23.06047289936396 30-31 23.20967253656959 25.559017570314136 27.4660819693443 23.76522792377197 32-33 22.95821349533219 25.52978977502931 27.565114042948043 23.94688268669046 34-35 23.785022264079178 25.645688602928086 27.329333350507145 23.23995578248559 36-37 23.84851298031373 25.216477453601797 27.424290657449934 23.510718908634537 38-39 23.31284020939413 25.48498305657969 27.439173696877937 23.763003037148245 40-41 23.733420450437045 25.4274460697751 27.48584523844519 23.35328824134266 42-43 23.41456533485094 25.42428917453122 27.246630308330687 23.914515182287143 44-45 23.442737309545837 25.567941972295866 27.596584302567834 23.39273641559046 46-47 23.94977667782545 25.97199465081345 26.8056762226961 23.27255244866499 48-49 23.572027499885603 26.03546548256469 26.665416139258248 23.72709087829146 50-51 23.726651476496095 26.121103377302806 26.8277523749667 23.324492771234397 52-53 24.113340208203724 25.762445449782284 26.680461862803 23.443752479210993 54-55 23.559800008545608 26.061238670630775 26.788297124100097 23.59066419672352 56-57 23.560239410340973 26.223317325976435 26.64858250495994 23.567860758722656 58-59 23.618225295543162 25.74506635118628 26.585672289292443 24.051036063978113 60-61 23.142125874371693 26.091330117720286 26.976148967511843 23.790395040396177 62-63 23.301401449298638 26.0761783316732 27.21107741017188 23.41134280885628 64-65 23.04042708642367 26.199589629026697 26.841085946688136 23.918897337861495 66-67 22.975003928103508 26.154017718815066 26.8448942306046 24.026084122476828 68-69 23.49425484505491 25.733330383758833 27.004682853567868 23.767731917618384 70-71 23.334657601039048 25.91387350728211 26.550749302183647 24.200719589495193 72-73 23.28294657389329 26.004161892591416 26.59723310204637 24.115658431468926 74-75 23.204778509676384 26.513564939494373 26.344152819701925 23.937503731127315 76-77 23.386842370818144 25.98928283869318 26.702689532934077 23.921185257554605 78-79 23.32305335155992 26.21887785266464 26.633173138550053 23.824895657225387 80-81 23.584061296319334 26.154256888343287 26.370354051155115 23.891327764182265 82-83 23.41994136230369 26.233762888550498 26.4648742016864 23.88142154745941 84-85 23.191614611546957 26.36244000560642 26.416549550539237 24.029395832307383 86-87 23.522271537415808 26.313494321883763 26.146729772335934 24.017504368364495 88-89 23.284055142239772 26.402537501835262 25.94554413665588 24.367863219269086 90-91 23.11819435874995 26.283866834594207 26.207711534755063 24.390227271900784 92-93 23.210064277530076 25.988731810483255 26.402909898424472 24.39829401356219 94-95 23.010054728267786 25.754225834409112 26.59952908234695 24.63619035497615 96-97 23.221903546442732 25.417927989913757 26.55893341107211 24.801235052571403 98-99 22.883517374641983 25.82734496415615 26.18682136045869 25.102316300743183 100-101 23.126928632966468 25.40031776325698 26.51061034121519 24.962143262561362 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 477.0 1 335.5 2 282.0 3 398.0 4 352.5 5 329.0 6 668.0 7 1507.5 8 2363.0 9 2646.0 10 2318.0 11 1932.5 12 1912.0 13 2075.5 14 2283.5 15 2475.0 16 2682.0 17 2926.5 18 3147.0 19 3463.5 20 4017.5 21 4747.0 22 5596.0 23 6608.5 24 8183.5 25 10635.5 26 13465.5 27 17080.5 28 20494.5 29 23601.5 30 27545.5 31 34169.5 32 44423.5 33 47452.5 34 45602.5 35 48449.0 36 53852.5 37 60044.5 38 65919.0 39 70857.5 40 76015.5 41 82614.5 42 87686.0 43 92207.5 44 97778.0 45 105680.0 46 111480.0 47 118557.5 48 126055.5 49 127703.5 50 128665.5 51 129407.0 52 131468.0 53 138020.5 54 143582.5 55 138363.0 56 128736.5 57 119436.5 58 111885.5 59 99973.5 60 79974.5 61 65273.0 62 55361.5 63 41965.5 64 29371.5 65 21766.0 66 16196.0 67 12360.5 68 9418.5 69 7264.0 70 5638.5 71 3758.0 72 2566.0 73 2091.5 74 1570.0 75 1079.5 76 759.5 77 461.5 78 278.0 79 180.0 80 92.0 81 48.0 82 32.0 83 17.5 84 8.0 85 5.0 86 5.0 87 2.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.060714418833549E-4 2 0.04587960815056996 3 9.697143070133677E-4 4 0.0 5 0.08500151972414052 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.5151786047083873E-4 26-27 0.008378937684037382 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010409277014346621 36-37 4.545535814125162E-5 38-39 0.004090982232712645 40-41 0.005091000111820181 42-43 3.0303572094167745E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 7.575893023541936E-5 68-69 9.091071628250322E-4 70-71 1.2121428837667097E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0011060803814371227 82-83 4.8485715350668387E-4 84-85 0.0046515983164547485 86-87 0.002393982195439252 88-89 7.575893023541936E-5 90-91 0.003181875069887613 92-93 9.697143070133677E-4 94-95 3.0303572094167742E-5 96-97 1.5151786047083871E-5 98-99 7.575893023541936E-5 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3299941.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.35018079770821 #Duplication Level Percentage of deduplicated Percentage of total 1 70.46846250578851 30.54820590162454 2 14.543621327317696 12.609372279852545 3 5.654032601003581 7.35310006468925 4 2.7105944770034114 4.7001904258946885 5 1.627297327959194 3.5271816689329265 6 1.046419265470948 2.721747860902238 7 0.6831947095414314 2.073162992506116 8 0.5274305223690537 1.8291366802342532 9 0.37113936908055817 1.4480062875710602 >10 2.1143139084835285 16.76550299269263 >50 0.165921281396182 4.945179878584995 >100 0.07862839495289042 6.175843259877147 >500 0.004821541912679196 1.3502350499748599 >1k 0.003913135463419146 3.336813879622983 >5k 2.0963225696888282E-4 0.6163207770397096 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8309 0.2517923805304398 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7036 0.21321593325456423 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5001 0.1515481640429329 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4700 0.1424267888425884 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4490 0.1360630387028132 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4015 0.1216688419580835 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3886 0.11775968115793585 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3692 0.1118807881716673 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3683 0.1116080560228198 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.0303572094167745E-5 2 3.0303572094167745E-5 0.0 0.0 0.0 6.060714418833549E-5 3 3.0303572094167745E-5 0.0 0.0 0.0 6.060714418833549E-5 4 3.0303572094167745E-5 0.0 0.0 0.0 6.060714418833549E-5 5 3.0303572094167745E-5 0.0 0.0 0.0 6.060714418833549E-5 6 3.0303572094167745E-5 0.0 0.0 0.0 6.060714418833549E-5 7 1.5151786047083873E-4 0.0 0.0 0.0 9.091071628250324E-5 8 1.8182143256500647E-4 0.0 0.0 0.0 9.091071628250324E-5 9 1.8182143256500647E-4 0.0 0.0 3.0303572094167745E-5 9.091071628250324E-5 10-11 2.1212500465917422E-4 0.0 0.0 3.0303572094167745E-5 1.5151786047083873E-4 12-13 2.1212500465917422E-4 0.0 0.0 3.0303572094167745E-5 4.848571535066839E-4 14-15 3.4849107908292904E-4 0.0 0.0 3.0303572094167745E-5 5.90919655836271E-4 16-17 4.0909822327126453E-4 0.0 0.0 3.0303572094167745E-5 6.666785860716903E-4 18-19 5.151607256008516E-4 0.0 0.0 3.0303572094167745E-5 7.272857302600259E-4 20-21 5.757678697891871E-4 0.0 0.0 7.575893023541936E-5 8.636518046837808E-4 22-23 6.060714418833549E-4 0.0 0.0 1.3636607442375485E-4 9.848660930604517E-4 24-25 6.969821581658581E-4 0.0 0.0 2.8788393489459355E-4 0.0010000178791075356 26-27 7.272857302600259E-4 0.0 0.0 5.454642976950194E-4 0.0010303214512017032 28-29 8.636518046837808E-4 0.0 0.0 0.00262125898614551 0.001060625023295871 30-31 0.0010000178791075356 0.0 0.0 0.010348669870158284 0.001075776809342955 32-33 0.0010151696651546195 0.0 0.0 0.026591384512632196 0.0011212321674842066 34-35 0.0011212321674842064 0.0 0.0 0.04994028681118844 0.001196991097719626 36-37 0.001181839311672542 0.0 0.0 0.09221376988255245 0.0012121428837667098 38-39 0.001303053600049213 0.0 0.0 0.15553308377331593 0.0012727500279550452 40-41 0.0013182053860962969 0.0 0.0 0.22354945133867543 0.001287901814002129 42-43 0.0014091161023788 0.0 0.0 0.2948537564762521 0.001303053600049213 44-45 0.0016212411070379742 0.0 0.0 0.38537052632153124 0.0013636607442375484 46-47 0.0017879107535558967 0.0 0.0 0.4929330554697796 0.0014394196744729679 48-49 0.0018788214698384 0.0 0.0 0.6078745044229579 0.0015606339628496389 50-51 0.0018788214698384 0.0 0.0 0.7317706589299626 0.0016970000372733937 52-53 0.0018788214698384 0.0 0.0 0.8555455991485909 0.0018182143256500645 54-55 0.0018788214698384 0.0 0.0 0.9934723075351954 0.0019697321861209035 56-57 0.0019697321861209035 0.0 0.0 1.1501569270480896 0.002106098260544658 58-59 0.0019697321861209035 0.0 0.0 1.3211296808033839 0.0021970089768271616 60-61 0.002015187544262155 0.0 0.0 1.509511836726778 0.0022121607628742455 62-63 0.0020606429024034065 0.0 0.0 1.69057567998943 0.002363678623345084 64-65 0.002121250046591742 0.0 0.0 1.882063952052476 0.002393982195439252 66-67 0.0023030714791567484 0.0 0.0 2.112749894619328 0.002393982195439252 68-69 0.002363678623345084 0.0 0.0 2.370890873503496 0.002393982195439252 70-71 0.0024545893396275874 0.0 0.0 2.639865379411329 0.0024697411256746713 72-73 0.002575803628004258 0.0 0.0 2.918082474807883 0.0026364107721925933 74-75 0.002606107200098426 0.0 0.0 3.200042061358067 0.0027576250605692645 76-77 0.002606107200098426 0.0 0.0 3.5095778985139434 0.0028182322047576 78-79 0.00262125898614551 1.5151786047083873E-5 0.0 3.861281156238854 0.0028939911349930196 80-81 0.00309096435360511 4.545535814125162E-5 0.0 4.231075646503983 0.0029091429210401035 82-83 0.003212178641981781 6.060714418833549E-5 0.0 4.608506636936842 0.002984901851275523 84-85 0.0033485447164055358 6.060714418833549E-5 0.0 5.0007257705516555 0.003075812567558026 86-87 0.0034849107908292904 6.060714418833549E-5 0.0 5.416884726120861 0.003166723283840529 88-89 0.0034849107908292904 6.060714418833549E-5 0.0 5.853453137495489 0.0032576340001230324 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6070 0.0 41.600025 1 GTATCAA 10000 0.0 36.85441 1 ATCAACG 12720 0.0 28.775314 3 TCAACGC 12810 0.0 28.49893 4 TATCAAC 12925 0.0 28.403671 2 CAACGCA 13095 0.0 27.91498 5 AACGCAG 13700 0.0 26.69467 6 TATAGCG 180 6.6556822E-9 26.408514 5 TAAGGTG 2040 0.0 25.165762 5 AGGTAAG 1835 0.0 25.137577 2 GTAAGGT 2030 0.0 24.821402 4 ACGCAGA 15565 0.0 23.404568 7 CGCAGAG 15730 0.0 23.068483 8 GGTAAGG 2125 0.0 22.59326 3 GTACATG 10920 0.0 22.557703 1 TACATGG 10760 0.0 22.318594 2 AAGGTAA 2025 0.0 21.604816 1 AGAGTAC 13980 0.0 20.82609 10-11 ACATGGG 11060 0.0 20.759098 3 GAGTACT 9175 0.0 20.188913 12-13 >>END_MODULE