FastQCFastQC Report
Thu 26 May 2016
SRR938501_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938501_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3301993
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85770.2597522163129964No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67650.20487626715138402No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA47670.1443673563208644No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46970.14224742450998534No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA45170.1367961712820106No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA43900.13295000928227285No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA39290.11898874407062644No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA34320.10393722821338508No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG33250.10069676101675563No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60900.045.3065071
GTATCAA104450.039.6014021
ATCAACG133700.030.4973533
TATCAAC134200.030.4545522
TCAACGC135550.029.9759454
CAACGCA136550.029.6520125
AACGCAG142250.028.5601756
TACATGG109250.026.2737312
GTACATG113350.026.0236641
ACGCAGA156800.025.7526047
CGCAGAG159050.025.1792188
GTAAGGT17500.024.7120974
TAAGGTG18550.024.0818715
ACATGGG117200.023.477793
GCAGAGT174850.021.9523459
AGGTAAG17600.021.8715022
AGAGTAC142600.021.65190310-11
GAGTACT97150.021.14684312-13
GTACTTT99650.020.40304814-15
AAGGTAA18700.020.3867361
Produced by FastQC (version 0.11.5)