FastQCFastQC Report
Thu 26 May 2016
SRR938501_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938501_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3301993
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83830.25387697672284587No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71170.2155364956860902No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50840.15396761895013103No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA46560.14100575016361333No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA45390.13746243556542972No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA41380.12531825476310823No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA39680.12016984893668764No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC36920.11181126065379302No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG36830.11153869799239428No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65900.040.9944841
GTATCAA106050.035.699731
ATCAACG135300.027.758393
TAAGGTG19400.027.6911955
TATCAAC136000.027.6280782
TCAACGC137750.027.2646854
CAACGCA138550.027.1072545
AACGCAG144700.026.028866
GTAAGGT18400.025.8373014
AGGTAAG18300.024.6907922
GTACATG111950.023.4093861
TACATGG108650.023.0260012
ACGCAGA166350.022.4414147
AAGGTAA19350.022.3677831
CGCAGAG167800.022.105978
ACATGGG110800.021.9682373
TATAACG1553.8699807E-521.4797332
GGTAAGG22400.021.4357343
AGAGTAC145500.021.13621710-11
GAGTACT96800.020.0677412-13
Produced by FastQC (version 0.11.5)