##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938501_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3301993 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.775844467265678 31.0 31.0 34.0 30.0 34.0 2 32.01561935473516 31.0 31.0 34.0 30.0 34.0 3 32.08590448253524 33.0 31.0 34.0 30.0 34.0 4 35.762185443760785 37.0 35.0 37.0 35.0 37.0 5 35.626348390199496 37.0 35.0 37.0 33.0 37.0 6 35.66261588077261 37.0 35.0 37.0 33.0 37.0 7 35.59286527863627 37.0 35.0 37.0 33.0 37.0 8 35.57245366661892 37.0 35.0 37.0 33.0 37.0 9 37.140887639677004 39.0 37.0 39.0 33.0 39.0 10-11 37.06885917080987 39.0 37.0 39.0 33.0 39.0 12-13 36.95731078170063 39.0 37.0 39.0 33.0 39.0 14-15 38.00280512405689 40.0 37.0 41.0 33.0 41.0 16-17 37.91465305953102 40.0 37.0 41.0 33.0 41.0 18-19 37.90130718023933 40.0 37.0 41.0 33.0 41.0 20-21 37.879232330292645 40.0 37.0 41.0 32.5 41.0 22-23 37.911116861846764 40.0 37.0 41.0 32.5 41.0 24-25 37.83204037682697 40.0 37.0 41.0 32.0 41.0 26-27 37.64972881529428 40.0 37.0 41.0 32.0 41.0 28-29 37.517434622060065 39.5 36.5 41.0 32.0 41.0 30-31 37.266249201618535 39.0 36.0 41.0 31.0 41.0 32-33 37.04684625315681 39.0 36.0 40.5 31.0 41.0 34-35 36.88677459340465 39.0 35.5 40.5 30.0 41.0 36-37 36.90762260852764 39.0 36.0 41.0 30.5 41.0 38-39 36.75644179136661 39.0 35.0 41.0 30.0 41.0 40-41 36.574334500406266 39.0 35.0 40.0 30.0 41.0 42-43 36.34220575270753 39.0 35.0 40.0 30.0 41.0 44-45 36.06115003877961 38.0 35.0 40.0 29.5 41.0 46-47 35.70295515465963 38.0 34.5 40.0 28.5 41.0 48-49 35.29082148266214 38.0 34.0 40.0 27.0 41.0 50-51 34.89682594723853 38.0 34.0 40.0 26.0 41.0 52-53 34.35058993159586 37.5 33.0 40.0 24.0 41.0 54-55 33.995570856752266 37.0 33.0 40.0 24.0 41.0 56-57 33.59946129504212 37.0 32.0 39.5 23.0 41.0 58-59 33.10580110254625 36.0 31.5 39.0 22.0 40.5 60-61 32.6592032448282 36.0 31.0 39.0 21.0 40.0 62-63 32.15001727744426 35.0 31.0 38.5 19.5 40.0 64-65 31.620073997734096 35.0 30.0 38.0 18.0 40.0 66-67 31.097639365074365 34.5 29.5 37.5 14.5 40.0 68-69 30.50429271049333 34.0 29.0 37.0 10.0 39.0 70-71 29.91864988811303 34.0 28.5 36.0 7.5 39.0 72-73 29.324671948123452 34.0 28.0 36.0 7.0 38.5 74-75 28.705300859208364 33.0 26.5 35.0 2.0 37.5 76-77 27.46469298996091 31.5 25.5 34.0 2.0 36.0 78-79 28.092426755598815 33.0 26.0 35.0 2.0 36.0 80-81 27.81430275594164 33.0 26.0 35.0 2.0 36.0 82-83 27.330868508806653 32.5 26.0 35.0 2.0 36.0 84-85 26.750200257844277 32.0 25.0 35.0 2.0 35.0 86-87 26.11241089850887 32.0 23.5 34.0 2.0 35.0 88-89 25.35277527844547 31.0 20.5 34.0 2.0 35.0 90-91 24.8069877798045 31.0 19.0 34.0 2.0 35.0 92-93 24.03087816963876 31.0 13.5 34.0 2.0 35.0 94-95 23.238993389749766 30.5 4.5 34.0 2.0 35.0 96-97 22.242880133301313 30.0 2.0 34.0 2.0 35.0 98-99 21.059062360217 29.0 2.0 34.0 2.0 35.0 100-101 17.952934636748168 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.27700551689091313 1101 2 0.308222825178774 1101 3 0.3195344972761305 1101 4 0.25559467496292854 1101 5 0.1610748262594086 1101 6 0.24133292942183715 1101 7 0.3296987985595763 1101 8 0.26341779647069785 1101 9 0.3477315984226834 1101 10-11 0.3519901432793304 1101 12-13 0.3457098899194406 1101 14-15 0.39611909941753254 1101 16-17 0.5140494307135128 1101 18-19 0.5344189836123476 1101 20-21 0.499945833426338 1101 22-23 0.45260800491041664 1101 24-25 0.45424634449340573 1101 26-27 0.3957668275162689 1101 28-29 0.4689990922830205 1101 30-31 0.5001507408105326 1101 32-33 0.4245263811270874 1101 34-35 0.39711200624960696 1101 36-37 0.6629947620638248 1101 38-39 0.7267906724905941 1101 40-41 0.7727585870557903 1101 42-43 0.7764007047838817 1101 44-45 0.7330454992405038 1101 46-47 1.0016148907818234 1101 48-49 1.0943023597322252 1101 50-51 1.1235634757154855 1101 52-53 1.2147698491242096 1101 54-55 1.1914830079177179 1101 56-57 1.3302063084474014 1101 58-59 1.3126996634294628 1101 60-61 1.433770884758161 1101 62-63 1.4348291658121397 1101 64-65 1.4158991463424009 1101 66-67 1.5526234396094303 1101 68-69 1.5418764442405894 1101 70-71 1.6400950496678988 1101 72-73 1.612793560790017 1101 74-75 1.6870966028052372 1101 76-77 1.5825231962041393 1101 78-79 1.7380249282388966 1101 80-81 2.0735425767035665 1101 82-83 2.2317198030618535 1101 84-85 2.3616802659122094 1101 86-87 2.6199154289857383 1101 88-89 2.733998004662517 1101 90-91 3.0586363589681973 1101 92-93 3.208679647276469 1101 94-95 3.070215145240063 1101 96-97 3.2809706325659924 1101 98-99 3.5888399599386 1101 100-101 4.140058649422922 1102 1 0.33570398856621964 1102 2 0.3306077861128358 1102 3 0.31817135998053203 1102 4 0.2105054859587625 1102 5 0.14783670205385846 1102 6 0.12661985148345423 1102 7 0.2739683442155538 1102 8 0.33446327859585523 1102 9 0.3856173747695948 1102 10-11 0.4047193927650241 1102 12-13 0.42951589803929124 1102 14-15 0.5931060887607416 1102 16-17 0.5149092846321253 1102 18-19 0.521392801102202 1102 20-21 0.568703981172618 1102 22-23 0.5328428371425247 1102 24-25 0.41187097824533936 1102 26-27 0.42667921150710697 1102 28-29 0.367835382328046 1102 30-31 0.335463707526408 1102 32-33 0.26593418247789913 1102 34-35 0.5544234893736188 1102 36-37 0.5646443332465836 1102 38-39 0.6678016406296337 1102 40-41 0.7398130111615444 1102 42-43 0.7556995549741359 1102 44-45 0.6710073828707621 1102 46-47 0.6211404206386746 1102 48-49 0.7212219872525125 1102 50-51 0.8530109403811892 1102 52-53 0.9589489288116866 1102 54-55 0.7920906053710368 1102 56-57 0.8853903929389872 1102 58-59 0.9705667380578404 1102 60-61 1.003337672389975 1102 62-63 1.1052410735593625 1102 64-65 1.1001830756914224 1102 66-67 1.046786550730129 1102 68-69 1.258899426160209 1102 70-71 1.3655577708012885 1102 72-73 1.3502417723574283 1102 74-75 1.4863968826120413 1102 76-77 1.5037651828751564 1102 78-79 1.628507287322936 1102 80-81 1.8359319181827978 1102 82-83 2.044414714658515 1102 84-85 2.271899200433168 1102 86-87 2.307055673848211 1102 88-89 2.482409666290618 1102 90-91 2.6010595527669977 1102 92-93 2.864942362866447 1102 94-95 2.9969317713783497 1102 96-97 3.213785896619399 1102 98-99 3.6249915968234987 1102 100-101 3.899726769984607 1103 1 0.29117256668429903 1103 2 0.2964723107563927 1103 3 0.3517162217174885 1103 4 0.20884953453816735 1103 5 0.19703025944614438 1103 6 0.14408263935477805 1103 7 0.26928357404936065 1103 8 0.25158150664635315 1103 9 0.3925628943601893 1103 10-11 0.40262840325934945 1103 12-13 0.45236950644103757 1103 14-15 0.6422450211836193 1103 16-17 0.6455630066900184 1103 18-19 0.5362418898158694 1103 20-21 0.5229460627400755 1103 22-23 0.5110313282952319 1103 24-25 0.43478539842242014 1103 26-27 0.45960177110642064 1103 28-29 0.5187460650707507 1103 30-31 0.6131577370776071 1103 32-33 0.7117637169615065 1103 34-35 0.6716405252417061 1103 36-37 0.7597819263014287 1103 38-39 0.8511419001676543 1103 40-41 0.6924394309957762 1103 42-43 0.8127408493384394 1103 44-45 0.9927567520429008 1103 46-47 1.0726523100197838 1103 48-49 1.1810174061792509 1103 50-51 1.2512548834227388 1103 52-53 1.362918383494538 1103 54-55 1.3605482165456735 1103 56-57 1.2987729973911328 1103 58-59 1.523457289235857 1103 60-61 1.6186799551644242 1103 62-63 1.5488825913126263 1103 64-65 1.524928730063852 1103 66-67 1.7174041720209523 1103 68-69 1.706525548725967 1103 70-71 1.7821510257342084 1103 72-73 1.8656090437919204 1103 74-75 1.7767832199716267 1103 76-77 1.7845292262455708 1103 78-79 1.9890296710358832 1103 80-81 2.3171090892914386 1103 82-83 2.3763323257184723 1103 84-85 2.5364996851769597 1103 86-87 2.7392574856467817 1103 88-89 2.903692369218607 1103 90-91 3.2281848337762504 1103 92-93 3.2594552092408122 1103 94-95 3.2380125852481427 1103 96-97 3.5348507937992117 1103 98-99 3.697160235270484 1103 100-101 4.013849346605181 1104 1 0.2454466847771819 1104 2 0.2186494620759305 1104 3 0.21336201395784116 1104 4 0.16837183758571683 1104 5 0.10345142464551316 1104 6 0.1895984719303172 1104 7 0.18803477790352474 1104 8 0.19866269733361008 1104 9 0.2937375892933076 1104 10-11 0.2316992116987464 1104 12-13 0.22897785083886646 1104 14-15 0.42230365209636744 1104 16-17 0.41234656747676723 1104 18-19 0.46966353912893766 1104 20-21 0.42733898968843675 1104 22-23 0.42079484989945115 1104 24-25 0.40686241193816386 1104 26-27 0.32062346439172273 1104 28-29 0.4399802330191491 1104 30-31 0.48728560367942464 1104 32-33 0.3164986480415024 1104 34-35 0.38552223307899425 1104 36-37 0.5744053892975955 1104 38-39 0.5701040869059852 1104 40-41 0.5811270980231029 1104 42-43 0.709792575962993 1104 44-45 0.7824382620051438 1104 46-47 0.6311637185086738 1104 48-49 0.756117091495824 1104 50-51 0.7666168246176923 1104 52-53 1.0030720614916717 1104 54-55 1.06485743812096 1104 56-57 1.1289569243972295 1104 58-59 1.1837078299818984 1104 60-61 1.311623174019445 1104 62-63 1.3551244191552456 1104 64-65 1.3079046651739397 1104 66-67 1.4870446894986706 1104 68-69 1.534124011374434 1104 70-71 1.5398950349462766 1104 72-73 1.7089816873099117 1104 74-75 2.054430284510783 1104 76-77 1.8910963495337647 1104 78-79 2.1613553810550172 1104 80-81 2.346431785459888 1104 82-83 2.534693708496558 1104 84-85 2.8782848129984053 1104 86-87 2.898713315939876 1104 88-89 2.9275405415607985 1104 90-91 3.0848475033136857 1104 92-93 3.2958652365862875 1104 94-95 3.610728215414234 1104 96-97 3.8866617524491183 1104 98-99 4.334839665506195 1104 100-101 4.6233806422409 1105 1 0.2351779728891934 1105 2 0.2684996180184456 1105 3 0.2718536359489292 1105 4 0.2206351133878357 1105 5 0.1472859609708479 1105 6 0.24557983042649312 1105 7 0.2239629916353536 1105 8 0.2040690104825984 1105 9 0.32070430266686856 1105 10-11 0.3529909558029871 1105 12-13 0.38930168274512056 1105 14-15 0.4340706246635406 1105 16-17 0.4241546012194348 1105 18-19 0.46286236030891104 1105 20-21 0.3470656508850851 1105 22-23 0.37197239266559023 1105 24-25 0.2829392896121945 1105 26-27 0.4720986804418672 1105 28-29 0.5517198794414995 1105 30-31 0.5497286329849587 1105 32-33 0.5245651586906988 1105 34-35 0.5173289212024059 1105 36-37 0.6147110481482159 1105 38-39 0.653551998758573 1105 40-41 0.6329903759120015 1105 42-43 0.7294733367919406 1105 44-45 0.5832678596559973 1105 46-47 0.7720267119825976 1105 48-49 0.7705008707394256 1105 50-51 0.8065726764333476 1105 52-53 0.8951450329121897 1105 54-55 0.8110431562435849 1105 56-57 0.9807212237154772 1105 58-59 1.0200424369162704 1105 60-61 1.179760853175253 1105 62-63 1.3974071758541484 1105 64-65 1.3011591640486024 1105 66-67 1.302234095301639 1105 68-69 1.270937887227383 1105 70-71 1.2624135502533278 1105 72-73 1.3885819196630926 1105 74-75 1.680742420683135 1105 76-77 1.5553356546041748 1105 78-79 1.8472089650884875 1105 80-81 2.006047413280317 1105 82-83 2.183050388983407 1105 84-85 2.273260760054402 1105 86-87 2.4553163037542873 1105 88-89 2.5720063496096515 1105 90-91 2.832942588583311 1105 92-93 2.993173989101898 1105 94-95 2.9825410721584973 1105 96-97 3.3223960656858935 1105 98-99 3.8842162012526273 1105 100-101 4.276037142886096 1106 1 0.11428155335114454 1106 2 0.19055541021835154 1106 3 0.19195259144556331 1106 4 0.13150151961480816 1106 5 0.09182956180303847 1106 6 0.09835951781463592 1106 7 0.09406607296551783 1106 8 0.07993401320941018 1106 9 0.21318319931953766 1106 10-11 0.2355194708675583 1106 12-13 0.12332285808175669 1106 14-15 0.18657701253794556 1106 16-17 0.16311078415056102 1106 18-19 0.27809145247949374 1106 20-21 0.4163344985303681 1106 22-23 0.34562802721475805 1106 24-25 0.2252006907775197 1106 26-27 0.2623229815386523 1106 28-29 0.40287794850123504 1106 30-31 0.455936968673889 1106 32-33 0.44798480541720664 1106 34-35 0.3991387975967342 1106 36-37 0.4571698352821798 1106 38-39 0.42086488772812913 1106 40-41 0.47756323835617565 1106 42-43 0.5602990652569133 1106 44-45 0.19882430379136196 1106 46-47 0.03807649560705073 1106 48-49 0.5676702801521145 1106 50-51 0.35507863640371795 1106 52-53 0.5985086668567803 1106 54-55 0.7002719622488698 1106 56-57 0.41168559093849666 1106 58-59 0.9294709834375254 1106 60-61 0.7821320113679491 1106 62-63 1.0420520007194582 1106 64-65 0.6660317546357355 1106 66-67 0.8713222753395762 1106 68-69 0.7346734231794336 1106 70-71 0.9734661072800961 1106 72-73 1.439589586187516 1106 74-75 1.4898215570373203 1106 76-77 1.7312409032467961 1106 78-79 1.935272798766416 1106 80-81 1.8238124371604272 1106 82-83 2.163969864808127 1106 84-85 2.212662255263645 1106 86-87 2.615297358070059 1106 88-89 2.9280718759096693 1106 90-91 2.903698617787942 1106 92-93 2.6541403157598644 1106 94-95 3.1106825180960556 1106 96-97 3.5406283346139062 1106 98-99 3.882657971137551 1106 100-101 4.063360145299509 1107 1 0.1486244669138621 1107 2 0.15552436393449653 1107 3 0.1262499601015179 1107 4 0.1294721424916574 1107 5 0.024613916192791407 1107 6 0.0631776791819334 1107 7 0.14460722703255868 1107 8 0.21516446876994877 1107 9 0.16038349535703844 1107 10-11 0.21706325098598 1107 12-13 0.29100618818069535 1107 14-15 0.37481747384667585 1107 16-17 0.3525045260574302 1107 18-19 0.29972835521166985 1107 20-21 0.24532952369943928 1107 22-23 0.23083528901617 1107 24-25 0.3482162200196015 1107 26-27 0.38706871693705125 1107 28-29 0.294876395638461 1107 30-31 0.25297911267777096 1107 32-33 0.29349874620496763 1107 34-35 0.31895114801673685 1107 36-37 0.3346971321643082 1107 38-39 0.46637235971722646 1107 40-41 0.4829290936845183 1107 42-43 0.48872220298237323 1107 44-45 0.5516030477929093 1107 46-47 0.5922710287963255 1107 48-49 0.5181277523650252 1107 50-51 0.5461482535798581 1107 52-53 0.701094904851864 1107 54-55 0.7788744050673628 1107 56-57 0.764749067407827 1107 58-59 0.8846493620967735 1107 60-61 0.8213303420141429 1107 62-63 0.9995851713059025 1107 64-65 1.0412225161730824 1107 66-67 1.2079903314374967 1107 68-69 1.2522902791308717 1107 70-71 1.2527959432490903 1107 72-73 1.4693624734247699 1107 74-75 1.301563541322441 1107 76-77 1.3394848385724991 1107 78-79 1.6613107052244303 1107 80-81 1.840236120125745 1107 82-83 1.953283330415669 1107 84-85 2.1368901093489647 1107 86-87 2.295460598248301 1107 88-89 2.6049168066930832 1107 90-91 2.7558374597992525 1107 92-93 2.8251406386731652 1107 94-95 2.852615939324302 1107 96-97 3.2166870035457897 1107 98-99 3.356661958269818 1107 100-101 3.6037623571195745 1108 1 0.16303341115670023 1108 2 0.21897207425866583 1108 3 0.12110320622279858 1108 4 0.12303163519356275 1108 5 0.07055368960374153 1108 6 0.07618916811223642 1108 7 0.10539105469339916 1108 8 0.0765910581171454 1108 9 0.16428475231487738 1108 10-11 0.11529983954240208 1108 12-13 0.19965027394604107 1108 14-15 0.3593236673286313 1108 16-17 0.26034618227334505 1108 18-19 0.35684570534818505 1108 20-21 0.3327116722820236 1108 22-23 0.2084714179955398 1108 24-25 0.3069947588012667 1108 26-27 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1.0553315242383228 2316 74-75 1.256889655814092 2316 76-77 1.1427730187198684 2316 78-79 1.2820497974721157 2316 80-81 1.3721042558184884 2316 82-83 1.5763679619994306 2316 84-85 1.7252580241607625 2316 86-87 1.8249255194458875 2316 88-89 2.0920287703358547 2316 90-91 2.1986342674791963 2316 92-93 2.197391777198206 2316 94-95 2.885099856780208 2316 96-97 3.127580374358036 2316 98-99 3.2543790412293205 2316 100-101 3.6147578030194047 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 11.0 4 42.0 5 114.0 6 244.0 7 574.0 8 1479.0 9 2642.0 10 4288.0 11 6664.0 12 9561.0 13 13037.0 14 17229.0 15 22742.0 16 28611.0 17 35234.0 18 34352.0 19 31801.0 20 32584.0 21 35000.0 22 38757.0 23 43599.0 24 49434.0 25 57688.0 26 67633.0 27 80937.0 28 95919.0 29 113456.0 30 133686.0 31 158493.0 32 187821.0 33 227969.0 34 281910.0 35 355904.0 36 443982.0 37 463987.0 38 214242.0 39 10362.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.01312238983159 18.332126990087797 14.698499995457285 23.956250624623333 2 16.569586798794262 19.879310914221186 38.12271117484465 25.4283911121399 3 19.88932707688897 22.69264338812265 29.353612466694244 28.064417068294134 4 14.066444114206178 16.143371594064554 34.49319850163219 35.296985790097075 5 14.696480877073528 36.45830996487607 33.4130841937473 15.432124964303103 6 34.680630758454065 34.71933465637268 15.945975657731559 14.6540589274417 7 30.499792095258833 31.56357387795795 19.223390237350593 18.713243789432624 8 27.66928942611326 34.56833494195778 18.491953193117006 19.270422438811956 9 26.732461274145646 16.292069668227644 18.85331071265142 38.12215834497529 10-11 26.033913457720836 25.71807390264001 26.279704408822184 21.968308230816962 12-13 26.82349720305282 22.734890716000912 26.799829678621368 23.6417824023249 14-15 23.21499470168471 24.32847374297886 24.695085664930243 27.76144589040619 16-17 23.320203888984622 27.523453259894858 25.68398842759509 23.47235442352543 18-19 23.520174028230826 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23.881893228833043 44-45 23.42030087199728 25.548271034653506 27.610582081088097 23.420846012261112 46-47 23.95252197121706 25.961688866155125 26.807814861974922 23.277974300652893 48-49 23.59832380020188 26.036336236933273 26.648345408363976 23.71699455450087 50-51 23.71304239591059 26.136366733666605 26.80629547064455 23.344295399778257 52-53 24.15033890138471 25.768513137368853 26.663018364969275 23.418129596277158 54-55 23.54088879049713 26.046814757027043 26.802494735755044 23.60980171672078 56-57 23.588314693580514 26.218286955787008 26.647981991482112 23.545416359150366 58-59 23.6387236435692 25.747631809031695 26.611519164334997 24.002125383064108 60-61 23.117901824746447 26.067544661663426 26.957416324020066 23.857137189570057 62-63 23.34391684052631 26.059549490262395 27.144182316558513 23.452351352652776 64-65 23.05816517478989 26.18965273397006 26.847982415468476 23.904199675771572 66-67 22.99601724658516 26.16276199754875 26.81350653409599 24.027714221770104 68-69 23.481217061489662 25.762147386451666 26.99384552205854 23.76279003000013 70-71 23.346930172171774 25.94458861663244 26.516258514176133 24.19222269701965 72-73 23.29922009211105 26.07263644602463 26.535841616841953 24.092301845022362 74-75 23.24856533614699 26.531779443505787 26.289425810412077 23.93022940993515 76-77 23.402275534805796 26.00212962292773 26.66055015864661 23.935044683619864 78-79 23.363102223414767 26.223177941322106 26.64050166066373 23.7732181745994 80-81 23.596562862139407 26.167662757460157 26.40710916427253 23.828665216127902 82-83 23.4347594406414 26.18915338327033 26.478390640954064 23.8976965351342 84-85 23.244795116436205 26.362516055388323 26.44763497807745 23.945053850098024 86-87 23.577456509365007 26.315508140425653 26.156283876798124 23.950751473411213 88-89 23.307900200848458 26.427350520534272 25.88136191729114 24.383387361326132 90-91 23.15251325309123 26.326940726667193 26.168565359026314 24.351980661215265 92-93 23.255864310771713 25.988855496532164 26.397185451848454 24.35809474084767 94-95 23.04970058991645 25.774342949848773 26.584035762643953 24.59192069759082 96-97 23.220918884582566 25.455736195645507 26.52577193921549 24.79757298055644 98-99 22.94970037792327 25.852205016788343 26.15670293668097 25.041391668607417 100-101 23.2110457663014 25.34319653322213 26.463224540736423 24.982533159740047 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 457.0 1 321.0 2 282.5 3 414.5 4 378.5 5 356.0 6 681.5 7 1533.0 8 2371.5 9 2671.5 10 2383.0 11 1994.5 12 2027.0 13 2156.5 14 2299.5 15 2512.5 16 2701.0 17 2935.5 18 3277.0 19 3569.5 20 4016.0 21 4797.5 22 5664.5 23 6807.5 24 8414.5 25 10607.0 26 13586.5 27 17108.0 28 20335.5 29 23789.5 30 27828.5 31 34396.5 32 44537.0 33 47729.5 34 45802.5 35 48120.5 36 53603.0 37 60079.5 38 65833.5 39 70594.5 40 75983.5 41 82669.5 42 87777.5 43 92293.5 44 97751.0 45 105668.5 46 111450.0 47 118621.5 48 125292.0 49 126724.5 50 127807.0 51 128787.5 52 131351.5 53 137631.5 54 143486.5 55 138080.0 56 128208.5 57 119617.5 58 111749.5 59 99637.0 60 80721.5 61 65937.5 62 55577.0 63 42678.5 64 30189.5 65 22230.0 66 16436.0 67 12495.0 68 9663.5 69 7533.0 70 5765.0 71 3847.5 72 2744.0 73 2186.5 74 1525.0 75 1076.0 76 797.5 77 505.5 78 309.0 79 185.5 80 109.5 81 71.0 82 36.0 83 15.0 84 7.0 85 4.5 86 4.0 87 3.0 88 1.5 89 2.0 90 1.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 9.085422046624569E-5 2 0.043791734264730424 3 3.331321417095675E-4 4 0.0 5 0.10257441490639138 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0011659624959834865 26-27 0.011250780967736757 28-29 0.0 30-31 0.0 32-33 4.694134724089361E-4 34-35 0.01440039394389994 36-37 0.003391890897406506 38-39 0.011477916518902372 40-41 0.006647500464113642 42-43 0.005572392188596402 44-45 0.0027407689840650783 46-47 7.571185038853808E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.8170844093249138E-4 68-69 2.1199318108790663E-4 70-71 0.0 72-73 1.5142370077707615E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.5427110233122845E-5 82-83 9.84254055050995E-4 84-85 0.004300433102068963 86-87 0.0014082404172268083 88-89 3.028474015541523E-5 90-91 8.328303542739188E-4 92-93 1.9685081101019898E-4 94-95 0.0 96-97 1.5142370077707615E-5 98-99 0.0 100-101 6.66264283419135E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3301993.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.21385372897555 #Duplication Level Percentage of deduplicated Percentage of total 1 70.29134777371169 30.37560021105728 2 14.78170002966038 12.775484458946746 3 5.560876251893406 7.209206788627663 4 2.7635551187406326 4.776954666928774 5 1.5665740055925532 3.3848849966645957 6 1.0156322867698095 2.6333631049737303 7 0.7018189217751163 2.1229810160872042 8 0.5271518810130597 1.8224211423242147 9 0.38602373382738725 1.5013415852576735 >10 2.153877361801839 16.97074831426001 >50 0.16539967245084208 4.975402323893663 >100 0.07691026441113914 6.062298640737161 >500 0.004917606806072562 1.3516163107857144 >1k 0.0040043369686692245 3.412799307029886 >5k 2.1075457729838022E-4 0.624897132425729 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8383 0.25387697672284587 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7117 0.2155364956860902 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5084 0.15396761895013103 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4656 0.14100575016361333 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4539 0.13746243556542972 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4138 0.12531825476310823 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3968 0.12016984893668764 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3692 0.11181126065379302 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3683 0.11153869799239428 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.028474015541523E-5 2 0.0 0.0 0.0 0.0 6.056948031083046E-5 3 0.0 0.0 0.0 0.0 6.056948031083046E-5 4 0.0 0.0 0.0 0.0 6.056948031083046E-5 5 3.028474015541523E-5 0.0 0.0 0.0 9.085422046624569E-5 6 9.085422046624569E-5 0.0 0.0 0.0 9.085422046624569E-5 7 1.5142370077707616E-4 0.0 0.0 0.0 9.085422046624569E-5 8 1.5142370077707616E-4 0.0 0.0 0.0 9.085422046624569E-5 9 1.5142370077707616E-4 0.0 0.0 0.0 1.2113896062166092E-4 10-11 1.8170844093249138E-4 0.0 0.0 6.056948031083046E-5 1.2113896062166092E-4 12-13 2.119931810879066E-4 0.0 0.0 7.571185038853808E-5 2.7256266139873706E-4 14-15 3.1798977163185994E-4 0.0 0.0 9.085422046624569E-5 3.4827451178727514E-4 16-17 3.4827451178727514E-4 0.0 0.0 9.085422046624569E-5 4.5427110233122847E-4 18-19 4.088439920981056E-4 0.0 0.0 9.085422046624569E-5 6.056948031083046E-4 20-21 4.239863621758132E-4 0.0 0.0 1.059965905439533E-4 7.116913936522579E-4 22-23 4.5427110233122847E-4 0.0 0.0 2.877050314764447E-4 8.176879841962112E-4 24-25 6.056948031083046E-4 0.0 0.0 4.694134724089361E-4 8.782574645070417E-4 26-27 6.056948031083046E-4 0.0 0.0 9.388269448178722E-4 9.84254055050995E-4 28-29 8.933998345847492E-4 0.0 0.0 0.0031344706060854764 0.0010296811652841177 30-31 0.001059965905439533 0.0 0.0 0.01078136749532782 0.001059965905439533 32-33 0.0010751082755172405 0.0 0.0 0.02678685266746477 0.0011962472361389015 34-35 0.0011508201259057787 0.0 0.0 0.0495155501541039 0.0012113896062166092 36-37 0.00133252856683827 0.0 0.0 0.09164162371028647 0.0012113896062166092 38-39 0.0013930980471491005 0.0 0.0 0.15218081928096153 0.0012719590865274395 40-41 0.001408240417226808 0.0 0.0 0.2188981018433413 0.0012719590865274395 42-43 0.0014839522676153463 0.0 0.0 0.29005209883848937 0.0013022438266828548 44-45 0.0016353759683924224 0.0 0.0 0.38067918375356946 0.0013628133069936853 46-47 0.0018322267794026214 0.0 0.0 0.4871906148801648 0.001408240417226808 48-49 0.0019382233699465747 0.0 0.0 0.6008341023133605 0.0014688098975376386 50-51 0.0019382233699465747 0.0 0.0 0.7267731942496547 0.001499094637693054 52-53 0.001953365740024282 0.0 0.0 0.8471247516272749 0.0016202335983147146 54-55 0.00196850811010199 0.0 1.5142370077707615E-5 0.9811195844449094 0.0016808030786255451 56-57 0.0021047894408013582 0.0 3.028474015541523E-5 1.134118091710067 0.0017110878187809606 58-59 0.002119931810879066 0.0 3.028474015541523E-5 1.3029252333363517 0.0017262301888586681 60-61 0.002119931810879066 0.0 3.028474015541523E-5 1.4878438567253172 0.0017565149290140834 62-63 0.002119931810879066 0.0 3.028474015541523E-5 1.6715359481379881 0.0019079386297911594 64-65 0.0021805012911898965 0.0 3.028474015541523E-5 1.8686441794395083 0.0019382233699465747 66-67 0.0023167826218892648 0.0 3.028474015541523E-5 2.0984145029986436 0.001953365740024282 68-69 0.002362209732122388 0.0 3.028474015541523E-5 2.351382937516827 0.00196850811010199 70-71 0.0024227792124332185 0.0 3.028474015541523E-5 2.6126342484675162 0.002044219960490528 72-73 0.0025287758029771718 0.0 3.028474015541523E-5 2.889951614070654 0.0021805012911898965 74-75 0.0025893452832880023 0.0 3.028474015541523E-5 3.174401035980391 0.002271355511656142 76-77 0.0026044876533657096 0.0 3.028474015541523E-5 3.482396843360964 0.0023016402518115575 78-79 0.0026196300234434173 0.0 3.028474015541523E-5 3.8308227788490163 0.0023470673620446802 80-81 0.0031344706060854764 0.0 3.028474015541523E-5 4.194890782627341 0.0024227792124332185 82-83 0.003179897716318599 0.0 3.028474015541523E-5 4.576417939105262 0.0025893452832880023 84-85 0.003346463787173383 0.0 3.028474015541523E-5 4.965122579000016 0.00266505713367654 86-87 0.003528172228105874 0.0 3.028474015541523E-5 5.375662516546825 0.002755911354142786 88-89 0.0035433145981835818 0.0 3.028474015541523E-5 5.809915405635325 0.0028467655746090316 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6590 0.0 40.994484 1 GTATCAA 10605 0.0 35.69973 1 ATCAACG 13530 0.0 27.75839 3 TAAGGTG 1940 0.0 27.691195 5 TATCAAC 13600 0.0 27.628078 2 TCAACGC 13775 0.0 27.264685 4 CAACGCA 13855 0.0 27.107254 5 AACGCAG 14470 0.0 26.02886 6 GTAAGGT 1840 0.0 25.837301 4 AGGTAAG 1830 0.0 24.690792 2 GTACATG 11195 0.0 23.409386 1 TACATGG 10865 0.0 23.026001 2 ACGCAGA 16635 0.0 22.441414 7 AAGGTAA 1935 0.0 22.367783 1 CGCAGAG 16780 0.0 22.10597 8 ACATGGG 11080 0.0 21.968237 3 TATAACG 155 3.8699807E-5 21.479733 2 GGTAAGG 2240 0.0 21.435734 3 AGAGTAC 14550 0.0 21.136217 10-11 GAGTACT 9680 0.0 20.06774 12-13 >>END_MODULE