FastQCFastQC Report
Thu 26 May 2016
SRR938500_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938500_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3287372
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80400.24457226015187813No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64200.19529277489739524No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA45860.13950353047966582No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA43740.13305461018710388No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42670.1297997306054806No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA42640.1297084722994538No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA37760.11486378785242436No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA34200.10403446887057503No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG33220.10105336420703223No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61200.041.6172941
GTATCAA102750.038.7140161
TATCAAC132150.030.2110612
ATCAACG132050.030.0539763
TCAACGC134250.029.5968744
CAACGCA135350.029.3198835
AACGCAG140100.028.286726
GTACATG108800.026.3030681
TACATGG105900.026.1653442
TAAGGTG18100.025.4698965
ACGCAGA157800.024.9286487
CGCAGAG158850.024.5545128
ACATGGG111800.024.019313
GTAAGGT18850.022.944784
AGGTAAG18900.022.6326072
TAAGGTA9250.022.6081374
GTGTAAG11850.022.539991
GCAGAGT173900.021.7448189
GGTAAGG19900.021.7341273
AGAGTAC140100.021.17510-11
Produced by FastQC (version 0.11.5)