FastQCFastQC Report
Thu 26 May 2016
SRR938500_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938500_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3287372
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82030.2495306281126687No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70060.21311856400796744No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47960.14589161190154326No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA45700.13901681951418943No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA45240.13761752548844486No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA41130.12511513756277048No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA38320.11656727623159167No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC36630.11142639165874746No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG36340.11054422803382154No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59750.043.1840131
GTATCAA97850.037.9272421
TATCAAC127550.028.902582
ATCAACG130200.028.2370473
TCAACGC133000.027.6774564
CAACGCA135000.027.2322355
TAAGGTG19350.026.5105615
AACGCAG139850.026.3557476
GTAAGGT19050.024.9333764
AGGTAAG17750.023.5526432
GTACATG107500.023.471841
TACATGG103350.023.3511682
ACGCAGA157650.023.2594497
GGTAAGG21050.023.2420523
CGCAGAG160500.022.7280568
ACATGGG106800.022.1041833
AGAGTAC141150.020.94761510-11
TAAGGTA9550.020.3918484
GCAGAGT176600.020.171899
GAGTACT94650.019.9476912-13
Produced by FastQC (version 0.11.5)