##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938500_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3287372 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.789756681020584 31.0 31.0 34.0 30.0 34.0 2 32.05075452367423 31.0 31.0 34.0 30.0 34.0 3 32.10964411694204 33.0 31.0 34.0 30.0 34.0 4 35.77265517866551 37.0 35.0 37.0 35.0 37.0 5 35.659073265818414 37.0 35.0 37.0 33.0 37.0 6 35.68702233881654 37.0 35.0 37.0 33.0 37.0 7 35.60380723568857 37.0 35.0 37.0 33.0 37.0 8 35.567878232217105 37.0 35.0 37.0 33.0 37.0 9 37.11233106566583 39.0 37.0 39.0 33.0 39.0 10-11 37.036549255758096 39.0 37.0 39.0 33.0 39.0 12-13 36.91406159692302 39.0 37.0 39.0 33.0 39.0 14-15 37.92690696398217 40.0 37.0 41.0 33.0 41.0 16-17 38.00931275803286 40.0 37.0 41.0 33.0 41.0 18-19 38.13162124639378 40.0 37.0 41.0 33.0 41.0 20-21 38.073680739508646 40.0 37.0 41.0 33.0 41.0 22-23 37.94664035588306 40.0 37.0 41.0 33.0 41.0 24-25 37.81828630894222 40.0 37.0 41.0 32.0 41.0 26-27 37.59243127945362 39.5 37.0 41.0 32.0 41.0 28-29 37.44614147714344 39.0 36.5 41.0 31.5 41.0 30-31 37.18014785062354 39.0 36.0 41.0 31.0 41.0 32-33 37.085872392902296 39.0 36.0 41.0 31.0 41.0 34-35 36.95890547221306 39.0 36.0 41.0 30.5 41.0 36-37 36.82809870620058 39.0 35.5 41.0 30.0 41.0 38-39 36.60608671607594 39.0 35.0 40.0 30.0 41.0 40-41 36.3704387577676 39.0 35.0 40.0 30.0 41.0 42-43 36.0941673166286 38.0 35.0 40.0 29.5 41.0 44-45 35.79504038484236 38.0 35.0 40.0 28.5 41.0 46-47 35.37193585027797 38.0 34.0 40.0 27.0 41.0 48-49 34.93772563616165 38.0 34.0 40.0 26.0 41.0 50-51 34.51160425409719 38.0 33.0 40.0 25.0 41.0 52-53 33.86987888806013 37.0 33.0 40.0 23.5 41.0 54-55 33.40484496430584 37.0 32.0 39.5 22.5 41.0 56-57 32.97820067214784 36.0 31.0 39.0 21.5 40.0 58-59 32.39159334568768 35.5 31.0 39.0 19.0 40.0 60-61 31.855800164995017 35.0 30.0 38.5 18.0 40.0 62-63 31.310851038458686 35.0 29.5 38.0 13.5 40.0 64-65 30.61523399238054 34.0 29.0 38.0 8.0 40.0 66-67 29.92535161825312 34.0 28.0 37.0 7.0 39.0 68-69 29.16604585669039 33.5 26.5 36.0 3.5 39.0 70-71 28.353452088780948 33.0 26.0 36.0 2.0 38.5 72-73 27.748893949330956 32.0 25.5 35.0 2.0 38.0 74-75 27.030915576332706 32.0 24.5 35.0 2.0 37.0 76-77 25.927724942598523 30.5 23.0 34.0 2.0 36.0 78-79 26.42239363236044 31.5 24.0 34.0 2.0 36.0 80-81 26.05887894646544 31.5 23.0 34.0 2.0 36.0 82-83 25.381714786157453 31.0 20.5 34.0 2.0 35.0 84-85 24.60434307404212 31.0 18.5 34.0 2.0 35.0 86-87 23.706185518401934 30.0 10.0 34.0 2.0 35.0 88-89 22.711621775691953 29.0 4.5 34.0 2.0 35.0 90-91 21.839674670222898 29.0 2.0 34.0 2.0 35.0 92-93 20.75175413673901 28.0 2.0 33.0 2.0 35.0 94-95 19.559362463390208 26.0 2.0 33.0 2.0 34.0 96-97 18.163880601282727 24.5 2.0 32.0 2.0 34.0 98-99 16.408266542393136 10.5 2.0 32.0 2.0 34.0 100-101 12.302301808252913 2.0 2.0 27.0 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.255904373311747 1101 2 0.2520074984052414 1101 3 0.30581908497741495 1101 4 0.1644956743633088 1101 5 0.14446098037296906 1101 6 0.16754923225703067 1101 7 0.2532074352757405 1101 8 0.2562824390173759 1101 9 0.30653624900923404 1101 10-11 0.30845466998050597 1101 12-13 0.3040983071116372 1101 14-15 0.3951248247970298 1101 16-17 0.35109036406391425 1101 18-19 0.29176193877876244 1101 20-21 0.3355427176430297 1101 22-23 0.42011031172803115 1101 24-25 0.32325041966567625 1101 26-27 0.3817482616874699 1101 28-29 0.3443408553644858 1101 30-31 0.48541018021956717 1101 32-33 0.4298265381451287 1101 34-35 0.2336426020425506 1101 36-37 0.3321460913825973 1101 38-39 0.3835621694524818 1101 40-41 0.46259501280086823 1101 42-43 0.48020085439476645 1101 44-45 0.5547959262141404 1101 46-47 0.6166332497724767 1101 48-49 0.5951612554496108 1101 50-51 0.9218705154713831 1101 52-53 0.9254402363964687 1101 54-55 0.8150862087988031 1101 56-57 1.0059237562355818 1101 58-59 1.01812960274453 1101 60-61 1.0008712797397656 1101 62-63 1.1891118573131578 1101 64-65 1.22834907398763 1101 66-67 1.423054687783079 1101 68-69 1.4341727359351815 1101 70-71 1.6414727462463539 1101 72-73 1.720555893059739 1101 74-75 1.6732374966868875 1101 76-77 1.6309117060847882 1101 78-79 1.7319678272699228 1101 80-81 1.8570564354008496 1101 82-83 2.069843580503118 1101 84-85 2.181070375573018 1101 86-87 2.268303412868974 1101 88-89 2.1696192342856833 1101 90-91 2.315774259770752 1101 92-93 2.4745878603820444 1101 94-95 2.292668069235326 1101 96-97 2.226062613786514 1101 98-99 2.32208551108517 1101 100-101 1.9821062384951027 1102 1 0.3021289364408837 1102 2 0.22422906516248986 1102 3 0.25520232003239585 1102 4 0.21383304066915798 1102 5 0.17663130494818802 1102 6 0.2358148056909073 1102 7 0.2600860917353671 1102 8 0.26034283756216325 1102 9 0.30827298971170336 1102 10-11 0.29661112505480247 1102 12-13 0.3184135069718863 1102 14-15 0.4510141155204437 1102 16-17 0.33891941438209017 1102 18-19 0.39674631525356574 1102 20-21 0.3243445124855242 1102 22-23 0.35778402719606817 1102 24-25 0.37680973938409323 1102 26-27 0.37407740680658463 1102 28-29 0.3451354341585571 1102 30-31 0.24642990916341034 1102 32-33 0.29851063286658075 1102 34-35 0.40533868597739797 1102 36-37 0.4976060125280881 1102 38-39 0.477106836423836 1102 40-41 0.6150402973444287 1102 42-43 0.6384871598408921 1102 44-45 0.5127396546148475 1102 46-47 0.478871139677544 1102 48-49 0.5184458242585279 1102 50-51 0.7063829199045841 1102 52-53 0.5242176872697186 1102 54-55 0.3611928917265175 1102 56-57 0.6146001944361359 1102 58-59 0.8098081195280145 1102 60-61 0.6897703697335906 1102 62-63 0.9516932143160943 1102 64-65 1.0236818770152993 1102 66-67 1.0664446719194451 1102 68-69 1.2479393530072969 1102 70-71 1.4360761719961062 1102 72-73 1.5422970695351985 1102 74-75 1.4473582283679534 1102 76-77 1.522352423624369 1102 78-79 1.615677300905329 1102 80-81 1.930180091007685 1102 82-83 2.0327994943427328 1102 84-85 2.110734860990231 1102 86-87 2.0749552508479674 1102 88-89 1.8232629330455694 1102 90-91 1.920565049839258 1102 92-93 2.189275288453384 1102 94-95 2.1344399276813846 1102 96-97 2.0409427278622587 1102 98-99 2.2666648950695247 1102 100-101 1.8903008478407362 1103 1 0.2471258763055708 1103 2 0.23290188552105917 1103 3 0.22894782121571922 1103 4 0.19164967067685978 1103 5 0.14363824195117303 1103 6 0.22328600595326975 1103 7 0.21798782065960154 1103 8 0.16211562721493777 1103 9 0.2845773071135369 1103 10-11 0.32078410520882983 1103 12-13 0.3518504462516958 1103 14-15 0.4755693514850776 1103 16-17 0.39311200864575113 1103 18-19 0.4547280463501764 1103 20-21 0.43094479614857306 1103 22-23 0.4777392426901699 1103 24-25 0.39170622743494476 1103 26-27 0.4665655579208732 1103 28-29 0.4217913015763699 1103 30-31 0.3367446331141366 1103 32-33 0.41325944066711173 1103 34-35 0.5390530218235909 1103 36-37 0.5701844846328186 1103 38-39 0.6626126569474167 1103 40-41 0.7423325266895588 1103 42-43 0.7111773843274065 1103 44-45 0.8478108941471945 1103 46-47 0.8551330538391824 1103 48-49 0.7790231349740182 1103 50-51 0.789067149450247 1103 52-53 1.0835770014651942 1103 54-55 1.0286961346344228 1103 56-57 1.1995508040610474 1103 58-59 1.3108828223397921 1103 60-61 1.2602683675904522 1103 62-63 1.3298862514116152 1103 64-65 1.528555116393079 1103 66-67 1.395448905976977 1103 68-69 1.3637231758578388 1103 70-71 1.553216689649851 1103 72-73 1.5273546272338905 1103 74-75 1.4496904065785365 1103 76-77 1.6232314031057982 1103 78-79 1.8080867031449692 1103 80-81 1.9895174724927145 1103 82-83 2.191970396111344 1103 84-85 2.1839399819647944 1103 86-87 1.9953854661854074 1103 88-89 1.8529399791063526 1103 90-91 1.9782073461313736 1103 92-93 2.2071880463977287 1103 94-95 1.7551794986775704 1103 96-97 1.8835515088839863 1103 98-99 2.1295415893714065 1103 100-101 1.84739524413766 1104 1 0.2814456085870738 1104 2 0.29388869795997863 1104 3 0.28138152160590835 1104 4 0.2036110081741569 1104 5 0.20952994872364883 1104 6 0.1890067845898784 1104 7 0.22813790103884912 1104 8 0.24956137750787377 1104 9 0.2428552522433165 1104 10-11 0.2778767567517946 1104 12-13 0.3381967302270823 1104 14-15 0.46966327870288893 1104 16-17 0.42696097612023465 1104 18-19 0.4636541382311705 1104 20-21 0.35270089136543703 1104 22-23 0.4550476805277057 1104 24-25 0.3872155223158771 1104 26-27 0.5458968316232529 1104 28-29 0.46073095483642845 1104 30-31 0.4921325014072764 1104 32-33 0.6358123157968123 1104 34-35 0.5544721757209388 1104 36-37 0.535744197446725 1104 38-39 0.7426732668686284 1104 40-41 0.7259638806873667 1104 42-43 0.7582808257438671 1104 44-45 0.8203233980516131 1104 46-47 0.8081489100919228 1104 48-49 0.9077729792165243 1104 50-51 0.8703370801234911 1104 52-53 0.9915461137061428 1104 54-55 1.075471577306999 1104 56-57 1.2106524719374505 1104 58-59 1.243291752398946 1104 60-61 1.2850962520656033 1104 62-63 1.4181753272653737 1104 64-65 1.6293574560802 1104 66-67 1.4671022431546596 1104 68-69 1.7595550773397584 1104 70-71 1.7780222512121995 1104 72-73 1.8938379051650536 1104 74-75 1.8839287538224774 1104 76-77 1.8089143138124726 1104 78-79 1.8625836665024273 1104 80-81 2.048727811326035 1104 82-83 2.209143253684452 1104 84-85 2.404547075789761 1104 86-87 2.227722007424685 1104 88-89 2.1922198961399886 1104 90-91 2.543556346293979 1104 92-93 2.665143161418005 1104 94-95 2.558309009009527 1104 96-97 2.7371302433899594 1104 98-99 2.809186696965611 1104 100-101 2.7632997467725477 1105 1 0.12476719913406242 1105 2 0.1526783360461721 1105 3 0.13044432431649966 1105 4 0.15841240882997454 1105 5 0.011065378094890832 1105 6 0.04977987255408323 1105 7 0.11076779766417388 1105 8 0.06356132946277882 1105 9 0.14758412600270532 1105 10-11 0.19466327120029092 1105 12-13 0.1658534119926287 1105 14-15 0.28748723158477674 1105 16-17 0.18776017094096886 1105 18-19 0.2543546922335054 1105 20-21 0.2125098096654625 1105 22-23 0.32899113003831815 1105 24-25 0.1932552676203514 1105 26-27 0.2002674868099703 1105 28-29 0.22832429126443543 1105 30-31 0.29084413333662695 1105 32-33 0.396871342728204 1105 34-35 0.4688494583045184 1105 36-37 0.44113713566567725 1105 38-39 0.3827694065159406 1105 40-41 0.4225749174934421 1105 42-43 0.44525581827215177 1105 44-45 0.6728292853420612 1105 46-47 0.5993427211771944 1105 48-49 0.6112166643600645 1105 50-51 0.6515714257245762 1105 52-53 0.6789597826768485 1105 54-55 0.7171785523949126 1105 56-57 0.8699133935412817 1105 58-59 0.7853901183711542 1105 60-61 0.9452522785101927 1105 62-63 0.9315969540581506 1105 64-65 1.0959549872688825 1105 66-67 1.2470293912473025 1105 68-69 1.3681306321369533 1105 70-71 1.3583086070412342 1105 72-73 1.5307643933441923 1105 74-75 1.376095725363534 1105 76-77 1.5373094769432285 1105 78-79 1.6109134812391268 1105 80-81 1.636080505419386 1105 82-83 1.960027848660772 1105 84-85 2.0587055906876266 1105 86-87 2.1775640768399533 1105 88-89 1.9929435930404438 1105 90-91 2.1917937448782006 1105 92-93 2.0861659460975552 1105 94-95 1.9284467394581775 1105 96-97 2.095403926618758 1105 98-99 2.194120726811505 1105 100-101 2.0762964924239355 1106 1 0.009273791027016642 1106 2 0.0645953962586816 1106 3 0.005134406961126103 1106 4 0.06799770989255194 1106 5 0.10155140721727918 1106 6 -0.07495940332155726 1106 7 0.003993889753907354 1106 8 0.006723470352191896 1106 9 -0.03380115538257655 1106 10-11 0.0861085683274112 1106 12-13 0.052733577281884436 1106 14-15 0.056174510215235784 1106 16-17 -0.03642814074733991 1106 18-19 0.07288086303240249 1106 20-21 0.04748988640690044 1106 22-23 0.08684482936929072 1106 24-25 -0.014262687908967564 1106 26-27 -0.30164367021482263 1106 28-29 0.015615220032636046 1106 30-31 0.14486690980259453 1106 32-33 -0.053270087810950884 1106 34-35 -0.09839153933420164 1106 36-37 -0.02611861994234488 1106 38-39 0.017644947641478836 1106 40-41 0.07563473646235508 1106 42-43 0.15460253216431852 1106 44-45 0.20808361434638556 1106 46-47 -0.39458683411599793 1106 48-49 -0.0360494218264833 1106 50-51 0.12917374764508338 1106 52-53 -0.04174687808662725 1106 54-55 0.045870504382314436 1106 56-57 0.1672348221127109 1106 58-59 0.38558812112597707 1106 60-61 0.3616164068061387 1106 62-63 0.3303745022675102 1106 64-65 0.6193094736915512 1106 66-67 0.3868685293818963 1106 68-69 0.3262241484691053 1106 70-71 0.31193906748987743 1106 72-73 0.574915318176938 1106 74-75 0.7416235687322867 1106 76-77 0.6657948173603856 1106 78-79 1.0162595556192926 1106 80-81 0.957573028048273 1106 82-83 1.2207888679445205 1106 84-85 1.3005628811244598 1106 86-87 1.338553844647901 1106 88-89 1.180731373441862 1106 90-91 1.3706209782508907 1106 92-93 1.3650632798244295 1106 94-95 1.2582344706663626 1106 96-97 1.2061452686101006 1106 98-99 1.259049642592693 1106 100-101 1.398417458578992 1107 1 -0.033794851323541764 1107 2 0.0478388106457146 1107 3 0.016104518409960633 1107 4 0.004022279294780162 1107 5 -0.06187817738251766 1107 6 -0.044445218924387575 1107 7 -0.03987895725723689 1107 8 -0.00741533410575812 1107 9 -0.06118717361965764 1107 10-11 0.02698749436590475 1107 12-13 0.07554766036192717 1107 14-15 0.035920584176743375 1107 16-17 -0.016359751689591917 1107 18-19 -0.024236664829800247 1107 20-21 0.1374031336206798 1107 22-23 0.1155036439589523 1107 24-25 0.10726403448212096 1107 26-27 0.10297005825528771 1107 28-29 0.1177086850782274 1107 30-31 0.1847067021024955 1107 32-33 0.17513480074021714 1107 34-35 0.14459288315819663 1107 36-37 0.11340835574154795 1107 38-39 0.11556668016427096 1107 40-41 0.11847161589498256 1107 42-43 0.2068669698542891 1107 44-45 0.11303422224912651 1107 46-47 0.2291315042831954 1107 48-49 0.17981367341160848 1107 50-51 0.23904456831377274 1107 52-53 0.18479757480091052 1107 54-55 0.4548788630449323 1107 56-57 0.33604814531432936 1107 58-59 0.4432002031928448 1107 60-61 0.4618608343643906 1107 62-63 0.6102719186808869 1107 64-65 0.6564763936510261 1107 66-67 0.7492203530900987 1107 68-69 0.8911386621469184 1107 70-71 0.798357507206191 1107 72-73 1.1754750952792676 1107 74-75 1.0245731634637956 1107 76-77 0.9687596096602888 1107 78-79 1.2416337957408743 1107 80-81 1.333097344157693 1107 82-83 1.4152607524430003 1107 84-85 1.5183484687637723 1107 86-87 1.8147240574138586 1107 88-89 1.8209580705036643 1107 90-91 1.7712947371161398 1107 92-93 1.7041799211618205 1107 94-95 1.5600689438173276 1107 96-97 1.674497598903919 1107 98-99 1.5468656253687811 1107 100-101 1.3945870381129328 1108 1 -0.01355938070321372 1108 2 0.02402089722830425 1108 3 0.00607170495725029 1108 4 0.008661090629459522 1108 5 0.002410597047060037 1108 6 0.029885996376783908 1108 7 0.020841013218678484 1108 8 0.0459499301668842 1108 9 -0.01584435727871636 1108 10-11 0.01290101607573746 1108 12-13 -9.38853228781511E-4 1108 14-15 0.1450259161622327 1108 16-17 0.06508688834122012 1108 18-19 0.0812909578395562 1108 20-21 0.05283692907011783 1108 22-23 0.08681385544125675 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41673.0 18 42667.0 19 41128.0 20 43367.0 21 47272.0 22 51476.0 23 57545.0 24 64256.0 25 73471.0 26 84375.0 27 97853.0 28 115232.0 29 134688.0 30 155785.0 31 181248.0 32 211692.0 33 251246.0 34 300849.0 35 361130.0 36 400116.0 37 318369.0 38 87198.0 39 1373.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.0767522173708 18.37927309792944 14.629109552570323 23.914865132129435 2 16.550151369996584 19.885980409366777 38.12589006501106 25.437978155625572 3 19.905253926230937 22.734677227267376 29.304571849410145 28.055496997091545 4 14.082342977916706 16.121814020439427 34.49989839908596 35.29594460255791 5 14.70556026382248 36.49020095748506 33.384762615130306 15.419476163562152 6 34.74964196324602 34.64721972444858 15.940787960717559 14.662350351587833 7 30.520245350997698 31.62656979496084 19.151985233189308 18.701199620852158 8 27.66390762220255 34.52221684811871 18.514040098924063 19.299835430754676 9 26.770163945895874 16.28605281129415 18.834111819040476 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23.826747804511054 25.629973827843 27.33070770084461 23.212570666801334 36-37 23.88243355364874 25.144718400100995 27.462129249170868 23.510718797079395 38-39 23.282062983202707 25.50143558501515 27.39706189938356 23.819439532398587 40-41 23.754860608184668 25.388289636345963 27.479003343918933 23.377846411550433 42-43 23.426057017140916 25.419259788356246 27.249832837575315 23.904850356927522 44-45 23.451182402141296 25.57153480100792 27.572566329551616 23.40471646729916 46-47 23.988465549735132 25.930093955060425 26.788774428916497 23.292666066287953 48-49 23.650548219063737 26.00930165493896 26.61131749008022 23.72883263591708 50-51 23.735783476892788 26.123070343119064 26.794564168582074 23.346582011406074 52-53 24.155693362357532 25.768242839569115 26.620823563624686 23.45524023444867 54-55 23.56160179012293 26.08687121506176 26.724416950682794 23.627110044132515 56-57 23.629531983227327 26.20803581219829 26.564049727875293 23.59838247669909 58-59 23.651339124382638 25.736728304554518 26.54723894953172 24.06469362153112 60-61 23.12739477004732 26.064573768955874 26.963331195861013 23.8447002651358 62-63 23.327919687823588 26.06051277433768 27.12365682983246 23.487910708006275 64-65 23.041377928881413 26.15989050208396 26.83683012719748 23.961901441837142 66-67 23.01854427100009 26.11419929642406 26.77815364627339 24.089102786302462 68-69 23.56587880975337 25.691734232785183 26.94118078303842 23.801206174423022 70-71 23.401540867483998 25.870749526445007 26.498394308640133 24.229315297430865 72-73 23.36209648729355 26.004075299810747 26.470832038603557 24.16299617429215 74-75 23.2653621190422 26.521139074008055 26.201233082231035 24.012265724718713 76-77 23.42106399884163 25.928005713986735 26.66748697743973 23.983443309731907 78-79 23.375799879052327 26.16886680302686 26.596898677727985 23.858434640192836 80-81 23.617662286462778 26.10374080646937 26.385912003713614 23.892684903354237 82-83 23.470799948225483 26.1521541104084 26.406418093216338 23.970627848149782 84-85 23.235209429678292 26.302133441593163 26.414905783986054 24.047751344742498 86-87 23.559996319080927 26.289154445736486 26.0961799817323 24.054669253450292 88-89 23.297165638692547 26.38527066605179 25.81403929947691 24.503524395778754 90-91 23.18052374779323 26.206097873150114 26.12772111319031 24.48565726586635 92-93 23.259125097323878 25.93369702056232 26.31264984530846 24.494528036805345 94-95 23.10334705250702 25.661592701764636 26.446460240252843 24.7886000054755 96-97 23.2504905514938 25.41562461072623 26.368909020474263 24.96497581730571 98-99 22.939843741444534 25.764212264386266 25.983627651510083 25.31231634265912 100-101 23.375262995150685 25.1721854898923 26.199587177654603 25.252964337302412 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 408.0 1 306.5 2 272.5 3 380.5 4 349.0 5 319.5 6 595.0 7 1373.0 8 2208.0 9 2544.5 10 2284.5 11 1889.0 12 1831.0 13 1980.0 14 2203.5 15 2429.0 16 2649.0 17 2942.0 18 3188.5 19 3501.5 20 4053.0 21 4764.0 22 5656.0 23 6783.5 24 8477.5 25 10773.0 26 13585.5 27 17001.0 28 20433.5 29 23759.5 30 27596.0 31 34257.5 32 43807.0 33 46702.5 34 45347.0 35 48130.0 36 53294.0 37 59294.5 38 65354.5 39 69967.5 40 74911.0 41 82013.5 42 87145.5 43 91461.0 44 97139.0 45 104798.5 46 110554.0 47 117274.5 48 124243.0 49 126418.5 50 127515.5 51 128476.5 52 130967.0 53 136463.5 54 141642.5 55 137138.0 56 128022.0 57 119620.5 58 111417.5 59 99214.0 60 80902.0 61 66476.0 62 55993.0 63 43084.0 64 30890.0 65 22954.5 66 17091.0 67 13081.0 68 10030.5 69 7759.5 70 5970.5 71 4087.5 72 2851.0 73 2244.5 74 1656.0 75 1191.0 76 869.5 77 541.0 78 325.5 79 200.5 80 109.0 81 60.5 82 36.0 83 20.0 84 11.0 85 6.5 86 4.0 87 2.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025004775851348734 2 0.011954838089513448 3 0.0017947466851941307 4 0.0 5 3.0419435342273404E-4 6 0.0 7 0.0 8 6.08388706845468E-5 9 2.1293604739591382E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0015970203554693536 26-27 0.008091569801044725 28-29 2.2814576506705053E-4 30-31 0.0 32-33 2.433554827381872E-4 34-35 0.00885205568460156 36-37 0.0012015676960197995 38-39 0.011696272889104123 40-41 0.010966206440889562 42-43 0.00456291530134101 44-45 5.323401184897846E-4 46-47 7.604858835568351E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 1.52097176711367E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 1.0646802369795691E-4 66-67 6.844372952011516E-4 68-69 0.0011103093899929792 70-71 9.12583060268202E-5 72-73 1.977263297247771E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.825166120536404E-4 82-83 0.0012319871313620728 84-85 0.006646646622286739 86-87 0.004547705583669874 88-89 0.0 90-91 0.0013840843080734399 92-93 7.14856730543425E-4 94-95 6.08388706845468E-5 96-97 1.52097176711367E-5 98-99 0.0 100-101 4.56291530134101E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3287372.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.304714322041995 #Duplication Level Percentage of deduplicated Percentage of total 1 70.35883458846743 30.468692318853897 2 14.75002326255611 12.774910872569325 3 5.500106819795474 7.14541663715874 4 2.7517801116339444 4.766602064455392 5 1.6258573906537257 3.520364491532012 6 1.0470020171837757 2.7204073949247074 7 0.7010869865128795 2.125226016608909 8 0.4948045600981167 1.7141896096234084 9 0.38426302532612916 1.497636048264445 >10 2.136457049820903 16.96145983151361 >50 0.16321432765362226 4.893779882106177 >100 0.07762297642500332 6.093898753611669 >500 0.004790457507634078 1.341251852874476 >1k 0.004015530556385811 3.51217925094046 >5k 1.408958089959934E-4 0.4639849749626796 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8203 0.2495306281126687 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7006 0.21311856400796744 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4796 0.14589161190154326 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4570 0.13901681951418943 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4524 0.13761752548844486 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4113 0.12511513756277048 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3832 0.11656727623159167 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3663 0.11142639165874746 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3634 0.11054422803382154 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.08388706845468E-5 2 3.04194353422734E-5 0.0 0.0 0.0 6.08388706845468E-5 3 3.04194353422734E-5 0.0 0.0 0.0 6.08388706845468E-5 4 3.04194353422734E-5 0.0 0.0 0.0 6.08388706845468E-5 5 6.08388706845468E-5 0.0 0.0 0.0 6.08388706845468E-5 6 9.12583060268202E-5 0.0 0.0 0.0 1.216777413690936E-4 7 1.5209717671136702E-4 0.0 0.0 0.0 1.216777413690936E-4 8 1.5209717671136702E-4 0.0 0.0 0.0 1.216777413690936E-4 9 1.5209717671136702E-4 0.0 0.0 0.0 1.216777413690936E-4 10-11 2.1293604739591382E-4 0.0 0.0 3.04194353422734E-5 1.216777413690936E-4 12-13 2.433554827381872E-4 0.0 0.0 4.56291530134101E-5 5.475498361609212E-4 14-15 3.3461378876500745E-4 3.04194353422734E-5 0.0 6.08388706845468E-5 6.540178598588781E-4 16-17 3.4982350643614413E-4 3.04194353422734E-5 0.0 6.08388706845468E-5 6.692275775300149E-4 18-19 4.258720947918276E-4 3.04194353422734E-5 0.0 9.12583060268202E-5 6.996470128722883E-4 20-21 4.867109654763744E-4 3.04194353422734E-5 0.0 1.216777413690936E-4 8.061150365702451E-4 22-23 5.475498361609212E-4 3.04194353422734E-5 0.0 1.825166120536404E-4 9.277927779393388E-4 24-25 7.300664482145616E-4 3.04194353422734E-5 0.0 3.6503322410728087E-4 9.582122132816122E-4 26-27 7.300664482145616E-4 3.04194353422734E-5 0.0 8.973733425970654E-4 0.0010342608016372958 28-29 9.582122132816122E-4 3.04194353422734E-5 0.0 0.0027225394631334695 0.0010342608016372958 30-31 0.0010646802369795691 3.04194353422734E-5 0.0 0.009506073544460439 0.0010798899546507058 32-33 0.0010950996723218425 3.04194353422734E-5 0.0 0.024731000933268278 0.0012015676960197995 34-35 0.0012319871313620726 3.04194353422734E-5 0.0 0.048549418806268355 0.0012471968490332095 36-37 0.0013992940257445765 3.04194353422734E-5 0.0 0.08943313990628379 0.0012471968490332095 38-39 0.001733907814509584 3.04194353422734E-5 0.0 0.15103249647438743 0.0012471968490332095 40-41 0.001779536967522994 3.04194353422734E-5 0.0 0.2164494921779464 0.0012624065667043462 42-43 0.0019164244265632243 3.04194353422734E-5 0.0 0.28728114737243005 0.0012928260020466196 44-45 0.002190199344643685 3.04194353422734E-5 0.0 0.3744632490633856 0.001368874590402303 46-47 0.002311877086012779 3.04194353422734E-5 0.0 0.47875628313436996 0.00147534261410026 48-49 0.002403135392039599 3.04194353422734E-5 0.0 0.5879012171424469 0.0015513912024559436 50-51 0.002403135392039599 3.04194353422734E-5 0.0 0.7117539481385131 0.001627439790811627 52-53 0.0024335548273818723 3.04194353422734E-5 0.0 0.835697937440606 0.0017947466851941307 54-55 0.0024639742627241457 3.04194353422734E-5 0.0 0.970988376125367 0.0018099564028652673 56-57 0.0025248131334086924 3.04194353422734E-5 0.0 1.126766304513149 0.0018707952735498143 58-59 0.0025248131334086924 3.04194353422734E-5 0.0 1.296415495417008 0.0019164244265632243 60-61 0.0025400228510798293 3.04194353422734E-5 0.0 1.481441710886386 0.0019164244265632243 62-63 0.002585652004093239 3.04194353422734E-5 0.0 1.6635628702805767 0.0019468438619054977 64-65 0.002585652004093239 3.04194353422734E-5 0.0 1.8568023332923684 0.0020076827325900444 66-67 0.002646490874777786 3.04194353422734E-5 0.0 2.0852979218658554 0.002022892450261181 68-69 0.0026769103101200596 3.04194353422734E-5 0.0 2.335421728967698 0.0020381021679323178 70-71 0.0027377491808046064 3.04194353422734E-5 0.0 2.598336908630967 0.0020381021679323178 72-73 0.0028290074868314264 3.04194353422734E-5 0.0 2.8723703919118373 0.0021597799093014116 74-75 0.0028746366398448367 3.04194353422734E-5 0.0 3.1550429948299126 0.002311877086012779 76-77 0.002889846357515973 3.04194353422734E-5 0.0 3.4580966194273115 0.002387925674368462 78-79 0.0029050560751871096 3.04194353422734E-5 0.0 3.8047108754348455 0.0024487645450530088 80-81 0.0033461378876500744 3.04194353422734E-5 0.0 4.164466327510242 0.0025248131334086924 82-83 0.003376557322992348 3.04194353422734E-5 0.0 4.539340238950748 0.00281379776916029 84-85 0.003452605911348031 3.04194353422734E-5 0.0 4.929788901286499 0.0028594269221737 86-87 0.003574283652717125 3.04194353422734E-5 0.0 5.330047831520131 0.00295068522820052 88-89 0.003619912805730535 4.56291530134101E-5 0.0 5.752421691247598 0.0030419435342273404 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5975 0.0 43.184013 1 GTATCAA 9785 0.0 37.927242 1 TATCAAC 12755 0.0 28.90258 2 ATCAACG 13020 0.0 28.237047 3 TCAACGC 13300 0.0 27.677456 4 CAACGCA 13500 0.0 27.232235 5 TAAGGTG 1935 0.0 26.510561 5 AACGCAG 13985 0.0 26.355747 6 GTAAGGT 1905 0.0 24.933376 4 AGGTAAG 1775 0.0 23.552643 2 GTACATG 10750 0.0 23.47184 1 TACATGG 10335 0.0 23.351168 2 ACGCAGA 15765 0.0 23.259449 7 GGTAAGG 2105 0.0 23.242052 3 CGCAGAG 16050 0.0 22.728056 8 ACATGGG 10680 0.0 22.104183 3 AGAGTAC 14115 0.0 20.947615 10-11 TAAGGTA 955 0.0 20.391848 4 GCAGAGT 17660 0.0 20.17189 9 GAGTACT 9465 0.0 19.94769 12-13 >>END_MODULE