FastQCFastQC Report
Thu 26 May 2016
SRR938499_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938499_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1688370
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57710.34180896367502384No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43930.260191782606893No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31940.1891765430563206No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38000.043.684291
GTATCAA64250.039.0140461
TATCAAC82400.030.188752
ATCAACG82550.029.9610393
TCAACGC84500.029.2696324
CAACGCA86700.028.5817785
AACGCAG88550.027.9060926
TACATGG67800.025.4652252
GTACATG70850.024.9067781
ACGCAGA99500.024.5954917
ACATGGG67700.024.3084933
CGCAGAG101450.024.0758938
GAGTACT55500.022.85799612-13
GTGTACG1703.1135096E-622.3833241
CATGGGG41350.021.7398684
GCAGAGT111500.021.3511529
AGAGTAC88450.021.32805310-11
AGTACTT57850.021.23132312-13
GTACTTT59850.021.03443314-15
GTATAGG4200.020.3848131
Produced by FastQC (version 0.11.5)