FastQCFastQC Report
Thu 26 May 2016
SRR938499_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938499_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1688370
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57690.34169050622790026No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47480.28121797947132443No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35200.20848510693746036No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37600.044.2402571
GTATCAA61400.037.231811
ATCAACG79950.028.461743
TATCAAC80450.028.35652
TCAACGC81850.027.859094
CAACGCA82800.027.4820795
AACGCAG86100.026.5832566
TAAGGTG8000.024.9404165
GTAAGGT7600.024.3778514
TACATGG63650.023.2966582
ACGCAGA98350.023.0790467
GTACATG67350.022.934181
CGCAGAG99350.022.7511528
ACATGGG63150.022.11663
GAGTACT55900.021.74665612-13
AGAGTAC87350.020.65784510-11
GTACTTT59450.020.44807414-15
CATGGGG40000.020.3086244
AGTACTT58350.020.22319812-13
AGGTAAG7400.019.9098242
Produced by FastQC (version 0.11.5)