FastQCFastQC Report
Thu 26 May 2016
SRR938498_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938498_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1704292
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59300.3479450704456748No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45000.2640392608778308No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32880.19292468661473502No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36200.042.205571
GTATCAA61150.039.618191
TATCAAC78700.030.5552162
ATCAACG79700.029.9333273
TCAACGC80950.029.4711074
CAACGCA82250.029.0044525
AACGCAG85400.028.1522396
ACGCAGA95750.024.80857
TACATGG64500.024.5354542
CGCAGAG96700.024.4665188
GTACATG69750.023.8152161
GAGTACT52550.022.68787412-13
ACATGGG66700.022.657443
AGAGTAC83450.021.71633310-11
GCAGAGT107250.021.306129
GTACTTT56950.020.89543514-15
AGTACTT57850.020.52718712-13
TAGACAG7100.019.4104885
TAAGGTG7200.019.14095
GTAAGGT7450.019.137034
Produced by FastQC (version 0.11.5)