##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938498_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1704292 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.104344208621526 31.0 31.0 34.0 28.0 34.0 2 31.206074428560363 31.0 31.0 34.0 28.0 34.0 3 31.273315253489425 31.0 31.0 34.0 27.0 34.0 4 34.918402480326144 37.0 35.0 37.0 32.0 37.0 5 34.92883027086908 37.0 35.0 37.0 32.0 37.0 6 34.96196719810924 37.0 35.0 37.0 32.0 37.0 7 34.88578600380686 37.0 35.0 37.0 32.0 37.0 8 34.84659494969172 37.0 35.0 37.0 32.0 37.0 9 36.354732639711976 39.0 35.0 39.0 32.0 39.0 10-11 36.195800074165696 39.0 35.0 39.0 32.0 39.0 12-13 36.04864600667022 38.0 35.0 39.0 30.0 39.0 14-15 37.039313979059926 39.0 36.0 41.0 31.0 41.0 16-17 36.8924383262962 39.0 36.0 41.0 31.0 41.0 18-19 36.878982298807955 39.0 36.0 41.0 30.5 41.0 20-21 36.96377440016147 39.0 36.0 41.0 31.0 41.0 22-23 36.88796286082432 39.0 36.0 41.0 30.0 41.0 24-25 36.76225934288256 39.0 36.0 41.0 30.0 41.0 26-27 36.566448120392515 39.0 36.0 41.0 30.0 41.0 28-29 36.382796199242854 39.0 35.5 40.5 30.0 41.0 30-31 36.15173309503301 39.0 35.0 40.0 29.0 41.0 32-33 35.87344656901517 38.0 35.0 40.0 28.5 41.0 34-35 35.62048375513116 38.0 34.5 40.0 27.0 41.0 36-37 35.32747029264938 38.0 34.0 40.0 26.5 41.0 38-39 35.06299037958284 38.0 34.0 40.0 25.5 41.0 40-41 34.99078532317232 38.0 34.0 40.0 25.0 41.0 42-43 34.79859671934152 38.0 33.5 40.0 24.5 41.0 44-45 34.4998879300026 38.0 33.0 40.0 24.0 41.0 46-47 34.125292496825665 38.0 33.0 40.0 23.0 41.0 48-49 33.73865863361443 38.0 33.0 40.0 21.5 41.0 50-51 32.98710549600655 37.0 31.5 39.5 20.0 40.5 52-53 33.05793080059051 37.0 32.0 39.0 20.0 40.0 54-55 33.17723430022555 37.0 32.0 40.0 19.5 41.0 56-57 32.854664869635016 37.0 31.5 40.0 17.5 41.0 58-59 32.51759851011447 36.5 31.0 39.5 14.5 41.0 60-61 32.050200904539835 36.0 31.0 39.0 11.5 40.5 62-63 31.509309730961597 35.0 30.0 39.0 8.0 40.0 64-65 30.99270576872977 35.0 29.5 38.5 7.0 40.0 66-67 30.436186991431043 35.0 29.0 38.0 2.0 40.0 68-69 29.859855294749963 34.0 28.0 37.5 2.0 39.5 70-71 29.222608567076534 34.0 27.5 36.5 2.0 39.0 72-73 28.665518291466487 34.0 26.0 36.0 2.0 39.0 74-75 28.046295470494492 33.5 26.0 35.5 2.0 38.5 76-77 27.31060375804146 33.0 25.5 35.0 2.0 37.5 78-79 26.5218967759046 32.0 23.5 35.0 2.0 37.0 80-81 25.874867393615645 32.0 21.0 35.0 2.0 36.0 82-83 25.21766985939029 31.0 19.5 34.5 2.0 36.0 84-85 24.54654836143102 31.0 17.5 34.0 2.0 35.0 86-87 23.875835830949157 30.5 9.0 34.0 2.0 35.0 88-89 23.068804230730414 30.0 4.5 34.0 2.0 35.0 90-91 22.210645241543116 29.0 2.0 34.0 2.0 35.0 92-93 21.34061093990936 29.0 2.0 34.0 2.0 35.0 94-95 20.35160406784753 28.0 2.0 34.0 2.0 35.0 96-97 19.093169186970307 25.5 2.0 33.0 2.0 35.0 98-99 17.60905026838124 22.5 2.0 32.5 2.0 34.0 100-101 14.288898263912522 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6180409180609381 1101 2 0.5073241369750967 1101 3 0.6167550349350961 1101 4 0.4472662480866987 1101 5 0.4053004807322722 1101 6 0.4589554071992481 1101 7 0.40471501481492567 1101 8 0.46336331437574785 1101 9 0.41792295718974515 1101 10-11 0.49592445015894526 1101 12-13 0.5353888453548024 1101 14-15 0.6427266603362582 1101 16-17 0.7405246862723871 1101 18-19 0.6353200307966276 1101 20-21 0.6651084827207256 1101 22-23 0.6085899836146496 1101 24-25 0.7341028640626774 1101 26-27 0.8007129916200313 1101 28-29 0.7306113083684451 1101 30-31 0.7727794774093866 1101 32-33 0.8652884973391508 1101 34-35 0.7709639763846496 1101 36-37 0.6042188013586198 1101 38-39 0.795300656337794 1101 40-41 0.7994927231329498 1101 42-43 0.7584695898517921 1101 44-45 0.5973939230361722 1101 46-47 0.699005078149824 1101 48-49 0.6614196801870449 1101 50-51 0.49992970974500395 1101 52-53 0.5456456371948093 1101 54-55 0.6558436664468843 1101 56-57 0.4915128725389408 1101 58-59 0.6013100956431359 1101 60-61 0.39228337506525435 1101 62-63 0.5144840581196313 1101 64-65 0.5247045495342881 1101 66-67 0.5420663332249127 1101 68-69 0.7084771298085393 1101 70-71 0.9341786016250389 1101 72-73 0.9608862732430588 1101 74-75 0.6357769296030291 1101 76-77 0.8782995039657315 1101 78-79 0.7803569411327764 1101 80-81 0.7498238056365913 1101 82-83 0.6367722890814207 1101 84-85 0.9889178577561104 1101 86-87 1.1232841988880828 1101 88-89 1.349918377122279 1101 90-91 1.2930856985613168 1101 92-93 1.3919711244489825 1101 94-95 1.2619174573519985 1101 96-97 1.4691607041843646 1101 98-99 1.5674961149228466 1101 100-101 1.2497089417959142 1102 1 0.4123561369213462 1102 2 0.5335772233927152 1102 3 0.5671189755528196 1102 4 0.5135903078505919 1102 5 0.48565778099357004 1102 6 0.534020471285082 1102 7 0.36920379897292577 1102 8 0.5443289971105045 1102 9 0.6214891154998554 1102 10-11 0.6547850396608297 1102 12-13 0.6897171582652675 1102 14-15 0.6149788887963226 1102 16-17 0.5598539307685115 1102 18-19 0.6250252314643276 1102 20-21 0.4827269971820769 1102 22-23 0.6883293954263294 1102 24-25 0.4327675682349721 1102 26-27 0.6021378164594609 1102 28-29 0.3977637016102804 1102 30-31 0.38464442203103033 1102 32-33 0.5127287799328073 1102 34-35 0.6560412184901097 1102 36-37 0.38081367082773454 1102 38-39 0.5747813673294999 1102 40-41 0.5842060419042809 1102 42-43 0.710462336273487 1102 44-45 0.7452948133831825 1102 46-47 0.3977036581643958 1102 48-49 0.13400029691403859 1102 50-51 0.4898782634020975 1102 52-53 0.2171597179651812 1102 54-55 0.319997874811655 1102 56-57 0.3746260071363068 1102 58-59 0.34955762573127913 1102 60-61 0.3745364089407488 1102 62-63 0.23328967888233976 1102 64-65 0.1803168605684462 1102 66-67 0.2183230319134708 1102 68-69 0.41272357033897933 1102 70-71 0.24230047307866087 1102 72-73 0.1853152838230372 1102 74-75 0.16996162968257167 1102 76-77 0.14481354498384746 1102 78-79 0.04844811001180105 1102 80-81 0.3181197505254332 1102 82-83 0.3950550189350608 1102 84-85 0.3165605104834448 1102 86-87 0.21589971464605284 1102 88-89 0.45228183933014776 1102 90-91 0.5099375819717267 1102 92-93 0.38064777849747244 1102 94-95 0.4514033637917656 1102 96-97 0.5530841281885763 1102 98-99 0.5621338801957521 1102 100-101 0.3686506535698779 1103 1 0.6115273351350972 1103 2 0.6181736824175701 1103 3 0.5484139724627077 1103 4 0.5120299612876451 1103 5 0.5843007197280414 1103 6 0.5873464828974448 1103 7 0.5394610326730529 1103 8 0.5144790935105519 1103 9 0.5769972262437975 1103 10-11 0.6424266109496486 1103 12-13 0.5305403007286316 1103 14-15 0.8126633385296671 1103 16-17 0.8486707861408789 1103 18-19 0.6997588752986701 1103 20-21 0.7000392825840152 1103 22-23 0.7353832709030783 1103 24-25 0.7467675702513574 1103 26-27 0.734663598472693 1103 28-29 0.6287816975176881 1103 30-31 0.8959262687447094 1103 32-33 1.0032037093677033 1103 34-35 1.0434205251961757 1103 36-37 0.8986534763674854 1103 38-39 0.6698094303792033 1103 40-41 1.0493602063627279 1103 42-43 0.7478311745764827 1103 44-45 1.072179352778491 1103 46-47 1.044496042006287 1103 48-49 0.8809847078991737 1103 50-51 1.0785303464462714 1103 52-53 1.0361559879874775 1103 54-55 1.0383015499100594 1103 56-57 1.2759392120101793 1103 58-59 1.2198954476514743 1103 60-61 1.1274817605160976 1103 62-63 1.2533125581401485 1103 64-65 1.2433411838945752 1103 66-67 1.2191618484159719 1103 68-69 1.2867789355887886 1103 70-71 1.2956950418090187 1103 72-73 1.300639614441753 1103 74-75 1.2056723267101823 1103 76-77 1.0297152176094926 1103 78-79 1.289492752457864 1103 80-81 1.1738690210138465 1103 82-83 0.9637583249004358 1103 84-85 1.0395753030259094 1103 86-87 1.2947543470068261 1103 88-89 1.3217542956678336 1103 90-91 1.2819855007086893 1103 92-93 1.2300444254494884 1103 94-95 1.1754991071676848 1103 96-97 0.9725928626678702 1103 98-99 1.1374087555915082 1103 100-101 0.9476738221790519 1104 1 0.4016800566174368 1104 2 0.479689316465052 1104 3 0.5241884368518193 1104 4 0.4257061338412029 1104 5 0.4008829432370149 1104 6 0.4768441667422678 1104 7 0.4527644490019469 1104 8 0.3179967541938211 1104 9 0.5683659291968937 1104 10-11 0.5397720799710655 1104 12-13 0.4190861895531839 1104 14-15 0.5839900611700841 1104 16-17 0.5664744196093281 1104 18-19 0.6881509569431401 1104 20-21 0.6854312194687822 1104 22-23 0.7723060416037129 1104 24-25 0.7499957115368261 1104 26-27 0.5905987461275899 1104 28-29 0.5696129800886212 1104 30-31 0.6385362861158725 1104 32-33 0.6889159270862919 1104 34-35 0.43887507065071674 1104 36-37 0.6124110762516821 1104 38-39 0.7417839526721934 1104 40-41 0.5801415468375382 1104 42-43 0.7632082735921202 1104 44-45 0.7649106063974855 1104 46-47 0.724463109174792 1104 48-49 0.7493492852008572 1104 50-51 0.6307560268168615 1104 52-53 0.6716402091803033 1104 54-55 0.81124596218568 1104 56-57 0.823136302340238 1104 58-59 0.8591054215947338 1104 60-61 0.8754533152352693 1104 62-63 0.7923723438957957 1104 64-65 0.8018639111672989 1104 66-67 0.8810502386881218 1104 68-69 1.0369797896710118 1104 70-71 1.269837340593039 1104 72-73 1.2902313920908561 1104 74-75 1.1761232531722534 1104 76-77 1.0942925192887074 1104 78-79 0.992767940645475 1104 80-81 0.9608786475338214 1104 82-83 0.9006050967591506 1104 84-85 0.8801858270560139 1104 86-87 1.0826940545922348 1104 88-89 1.1600830449440345 1104 90-91 0.7299787693826936 1104 92-93 0.795718281847865 1104 94-95 0.7712178390727225 1104 96-97 0.6529688759857599 1104 98-99 0.2816636050414232 1104 100-101 0.24997188239908752 1105 1 0.4605076619389443 1105 2 0.4727325537156055 1105 3 0.4324755765413997 1105 4 0.462970546258056 1105 5 0.43339402971151486 1105 6 0.3951091334828405 1105 7 0.3761014778267793 1105 8 0.32791914887231144 1105 9 0.3783021819685146 1105 10-11 0.42352346578038436 1105 12-13 0.5149980520808981 1105 14-15 0.5281558039638767 1105 16-17 0.6110212599640903 1105 18-19 0.5991473538389229 1105 20-21 0.6972729600452823 1105 22-23 0.5376538797411854 1105 24-25 0.7367473745079991 1105 26-27 0.7466963071031927 1105 28-29 0.672488534412345 1105 30-31 0.7888411641646513 1105 32-33 0.5826520689931698 1105 34-35 0.7543407025797606 1105 36-37 0.6499457214844924 1105 38-39 0.6483249726278473 1105 40-41 0.7798158816490641 1105 42-43 0.8865868766963345 1105 44-45 0.8375062272400555 1105 46-47 0.9283087300173634 1105 48-49 0.705772511364934 1105 50-51 0.6645281997658614 1105 52-53 0.7547749098454872 1105 54-55 0.7458357626291416 1105 56-57 0.8699835861539853 1105 58-59 0.7678568074040513 1105 60-61 0.8287030380733995 1105 62-63 0.725728774051003 1105 64-65 0.9606360841162989 1105 66-67 0.9868013473355717 1105 68-69 0.82714747259784 1105 70-71 0.7477336820564524 1105 72-73 0.7426704164811007 1105 74-75 0.7684874438596161 1105 76-77 0.8682393042111016 1105 78-79 0.8467452133727456 1105 80-81 0.7584881264695191 1105 82-83 0.9426851965374183 1105 84-85 0.7827980776103409 1105 86-87 0.778294110024607 1105 88-89 0.6610392533697542 1105 90-91 0.5375799334625135 1105 92-93 0.7825392352846734 1105 94-95 0.909286020890903 1105 96-97 1.0936631664513925 1105 98-99 0.8458750795425303 1105 100-101 0.8577462726429896 1106 1 0.13922057267072674 1106 2 0.28648133831181966 1106 3 -0.029119842460108458 1106 4 0.2527405501548188 1106 5 0.14661631570395883 1106 6 0.03955855089825633 1106 7 0.07891548774422574 1106 8 0.15607163551687364 1106 9 0.22752861869717123 1106 10-11 0.020605594084692314 1106 12-13 -0.028995518884364913 1106 14-15 0.25805308755975176 1106 16-17 0.025972562889400308 1106 18-19 -0.22705644058633823 1106 20-21 -0.029442797937164755 1106 22-23 0.16028314205800598 1106 24-25 0.25816400309927445 1106 26-27 0.11976520011155856 1106 28-29 0.05556108763800438 1106 30-31 0.07625832684885836 1106 32-33 0.09525429674582853 1106 34-35 0.3250804710896986 1106 36-37 -0.20173237609888872 1106 38-39 0.012117268990905927 1106 40-41 0.23715832112164748 1106 42-43 -0.08233846981046611 1106 44-45 0.0869170443026448 1106 46-47 -0.27879747604291083 1106 48-49 0.05643720184625067 1106 50-51 -0.1819459154015064 1106 52-53 0.12347364034767594 1106 54-55 -0.12910292250626298 1106 56-57 -0.610503347243764 1106 58-59 -0.9197772605041905 1106 60-61 -0.13816374744657978 1106 62-63 -0.1781909887569526 1106 64-65 -0.052151644444975886 1106 66-67 -0.5795404024225945 1106 68-69 -0.7032745043925885 1106 70-71 -0.7298611816875997 1106 72-73 -0.050987790186720616 1106 74-75 -0.02964565857293522 1106 76-77 0.018909690438722038 1106 78-79 0.13035120592034488 1106 80-81 0.017162623594256132 1106 82-83 -0.13614439918286436 1106 84-85 -0.020960585837659806 1106 86-87 0.07237825269340448 1106 88-89 0.027882353299911955 1106 90-91 0.1860958958693466 1106 92-93 0.2531442044653964 1106 94-95 -0.30283944488419934 1106 96-97 -0.17396361214654377 1106 98-99 -0.19741368172290663 1106 100-101 -0.4281720995313911 1107 1 0.368940441363776 1107 2 0.34902938650736814 1107 3 0.38649873890931374 1107 4 0.34780260010902 1107 5 0.3716968203711133 1107 6 0.4267073653414215 1107 7 0.39368205199819784 1107 8 0.30056035164505346 1107 9 0.3737264160882816 1107 10-11 0.46483333659833903 1107 12-13 0.4661790077836372 1107 14-15 0.42511165025515396 1107 16-17 0.482253549187611 1107 18-19 0.4671186535723493 1107 20-21 0.38341643883742904 1107 22-23 0.5239712186551344 1107 24-25 0.42856592409579974 1107 26-27 0.6358879138886664 1107 28-29 0.6387133436763435 1107 30-31 0.6914431638822975 1107 32-33 0.3752661873129597 1107 34-35 0.5541943955206037 1107 36-37 0.6214723450390736 1107 38-39 0.5380726826687834 1107 40-41 0.4448134970783073 1107 42-43 0.4768151489265193 1107 44-45 0.522758902764032 1107 46-47 0.5530708661182189 1107 48-49 0.5300868879539067 1107 50-51 0.45441993313525586 1107 52-53 0.34588597745696603 1107 54-55 0.3597952593304896 1107 56-57 0.38692056349106707 1107 58-59 0.2615050701671393 1107 60-61 0.2535020104539072 1107 62-63 0.24046046831818657 1107 64-65 0.13698873484549168 1107 66-67 0.27684374761365405 1107 68-69 0.2416518615130272 1107 70-71 0.4842765764508137 1107 72-73 0.13944949608424295 1107 74-75 0.37958797665102395 1107 76-77 0.3899817551464828 1107 78-79 0.2510267932161234 1107 80-81 0.21449917260799722 1107 82-83 0.4008052828090527 1107 84-85 0.2443823164280694 1107 86-87 0.11712559917125276 1107 88-89 0.3120362649450108 1107 90-91 0.30203313121935693 1107 92-93 0.23666294112117825 1107 94-95 0.13304447217744553 1107 96-97 0.1060230432604321 1107 98-99 -0.05197023536910095 1107 100-101 0.12935955932889875 1108 1 0.29489822103913 1108 2 0.28595515969781715 1108 3 0.2201841374333604 1108 4 0.192699088262394 1108 5 0.2442381050243796 1108 6 0.2062411298119997 1108 7 0.209229730576574 1108 8 0.012291947791908342 1108 9 -0.1213723157456954 1108 10-11 0.09213349828211648 1108 12-13 0.25187764290573966 1108 14-15 0.27360528807002993 1108 16-17 0.20288170252597126 1108 18-19 0.1308877666752366 1108 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-1.2250340156820716 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5076.0 3 1795.0 4 1881.0 5 2118.0 6 2614.0 7 3234.0 8 4302.0 9 5333.0 10 6792.0 11 8308.0 12 10184.0 13 12099.0 14 14054.0 15 16336.0 16 18905.0 17 21854.0 18 20867.0 19 19830.0 20 21057.0 21 22972.0 22 25491.0 23 28363.0 24 31956.0 25 35589.0 26 40288.0 27 45809.0 28 52570.0 29 60486.0 30 70114.0 31 82325.0 32 97307.0 33 116740.0 34 141605.0 35 173154.0 36 202236.0 37 191289.0 38 84984.0 39 4375.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.829625158184975 18.04310860144205 14.495239315571162 23.632026924801817 2 15.718673427815656 19.647916179911682 39.69102848441229 24.942381907860366 3 19.498007621990592 23.45364697926377 29.16010172046596 27.88824367827967 4 13.329075117971742 15.87637242320242 35.57791770920537 35.216634749620454 5 14.455303941651094 36.84475341856933 33.43314129108052 15.266801348699063 6 33.53939425252669 34.89400652177231 16.80066791215217 14.765931313548824 7 29.77275595404053 32.00299352869532 19.842927788457306 18.381322728806847 8 27.360732040875018 34.45475515486609 19.09457484460566 19.08993795965323 9 26.853791640423267 16.540884231831008 19.22525377097833 37.38007035676739 10-11 25.694018257482572 26.21239856994891 26.579274003412234 21.514309169156284 12-13 26.537860895989695 23.260818331739184 27.077269668286647 23.124051103984474 14-15 23.173529047507525 24.630262562444642 25.210760757852423 26.985447632195402 16-17 22.927176713170596 27.929621353655836 25.92856719415728 23.214634739016287 18-19 23.013749368618498 26.563720297921083 28.14604085276785 22.276489480692565 20-21 23.429790466529415 25.639393208257825 28.42570757832189 22.50510874689087 22-23 23.136359513836364 25.489367335084435 28.480003381272855 22.89426976980635 24-25 23.082130613045333 25.606801033142645 28.645165760210624 22.6659025936014 26-27 22.573429094077063 25.986666760595796 28.389755385074196 23.05014876025295 28-29 22.67232310994022 26.412555237786638 28.32788586617076 22.587235786102383 30-31 22.756168187401943 25.933201146532152 28.200287953927372 23.110342712138536 32-33 22.520062226657668 25.848219789251225 28.304881270363087 23.32683671372802 34-35 23.202284507340732 26.041687151759536 28.058036677390824 22.697991663508905 36-37 23.335836026810718 25.552040565941375 28.150223833547205 22.961899573700702 38-39 22.705941044414775 26.003139993837394 28.128270215544436 23.162648746203395 40-41 23.069794466681532 25.783485732395857 28.303000246499128 22.843719554423487 42-43 22.915222705091452 25.728035400411404 28.03158571189629 23.32515618260086 44-45 22.73201893757916 26.103129828975604 28.0778040294432 23.08704720400204 46-47 23.2249002924403 26.266343956385406 27.554700889057237 22.954054862117054 48-49 22.971132046629307 26.47188008601499 27.42436498805983 23.132622879295873 50-51 23.131782126945428 26.418391492570375 27.41979541210246 23.030030968381737 52-53 23.363944969471497 26.17952110882583 27.329071176455056 23.12746274524762 54-55 23.06230470182645 26.48966478398005 27.25721411281449 23.190816401379006 56-57 22.915011401524975 26.655128221678975 27.203316828603917 23.226543548192126 58-59 23.034900769101448 26.208628852879016 27.323448923982685 23.43302145403685 60-61 22.717615602030737 26.38527467246128 27.59531873354531 23.301790991962672 62-63 22.788362717579997 26.474397961072732 27.758673347691726 22.97856597365554 64-65 22.788136264548744 26.51572452521293 27.371940252965494 23.32419895727283 66-67 22.59015943261213 26.623914246366837 27.3026367904351 23.483289530585935 68-69 22.968886797280074 26.23925866289864 27.495563807239208 23.296290732582076 70-71 23.007722794009332 26.202039183833488 27.085298168866206 23.704939853290977 72-73 22.79522621543886 26.377119825255445 27.163223830424542 23.66443012888115 74-75 22.634243819847207 26.69154035420796 27.147013470231535 23.527202355713296 76-77 22.997374852157552 26.38169420768625 27.043776034721077 23.577154905435115 78-79 22.854325470370938 26.56052190643954 27.12362623579733 23.46152638739219 80-81 22.971910008517273 26.393225846864492 26.99292652463775 23.641937619980478 82-83 22.941524400333765 26.414418981831922 27.097907637492824 23.546148980341485 84-85 22.82263771418915 26.56986958241258 26.969789076280147 23.637703627118125 86-87 23.052643253104595 26.464394082981208 26.702193268390634 23.780769395523567 88-89 23.001922488300288 26.47642904914744 26.64214865579888 23.87949980675339 90-91 22.78472208159481 26.415550623079703 26.777562263214012 24.022165032111474 92-93 23.046741345928684 26.07821192328933 26.78185865559722 24.093188075184766 94-95 23.019095172999933 25.83072190471607 26.988417758927625 24.16176516335638 96-97 22.999592941063913 25.843576084656522 26.92869483757116 24.22813613670841 98-99 22.765945584299732 25.93337715385699 26.66433870134748 24.636338560495798 100-101 23.330596634594908 25.265888151759004 26.8308587995635 24.57265641408259 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 431.0 1 302.5 2 259.0 3 377.5 4 321.5 5 300.0 6 527.5 7 1181.0 8 1961.0 9 2204.0 10 1880.0 11 1565.0 12 1537.0 13 1599.0 14 1683.5 15 1790.0 16 1916.5 17 2051.5 18 2232.0 19 2416.5 20 2609.5 21 2924.5 22 3542.0 23 4241.5 24 5040.5 25 6289.0 26 8060.0 27 10163.0 28 11857.5 29 13713.0 30 16177.5 31 19382.5 32 23924.0 33 26592.0 34 27443.0 35 29435.5 36 32147.0 37 35203.0 38 38261.0 39 40166.5 40 42310.5 41 45557.0 42 47896.5 43 50732.5 44 54293.5 45 56978.5 46 58560.5 47 61171.0 48 64415.5 49 65991.0 50 65724.0 51 64619.0 52 64130.0 53 64252.5 54 65337.5 55 63106.0 56 58183.0 57 54133.0 58 50654.0 59 45540.0 60 37329.5 61 30932.0 62 26074.0 63 19749.0 64 14373.5 65 11237.0 66 8687.0 67 6804.5 68 5597.5 69 4593.0 70 3574.0 71 2424.0 72 1750.0 73 1269.5 74 864.5 75 629.5 76 459.5 77 298.5 78 195.5 79 139.5 80 78.0 81 44.5 82 29.0 83 21.0 84 12.5 85 8.5 86 7.0 87 6.5 88 5.5 89 4.0 90 3.5 91 3.0 92 3.0 93 2.5 94 1.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17373783365761267 2 0.04893527634935797 3 0.046940313044947696 4 0.05386400921907748 5 0.0599075745236145 6 0.06301737026284228 7 0.03620271643591591 8 0.032975569914075756 9 0.040427344609961204 10-11 0.04204091787088128 12-13 0.03916582369687824 14-15 0.03420775313150563 16-17 0.03315159608799431 18-19 0.04028065613169574 20-21 0.04934600408850126 22-23 0.04644157221884513 24-25 0.06683127069774429 26-27 0.05163434434944246 28-29 0.0438891926970261 30-31 0.052485137523382144 32-33 0.04940467947980745 34-35 0.04796713239280593 36-37 0.04658826069711059 38-39 0.02740140773998822 40-41 0.025347769044271752 42-43 0.02094711469630791 44-45 0.0036085365653303544 46-47 0.010766934304684879 48-49 0.010004154217704479 50-51 0.007891840130681832 52-53 0.00862528252200914 54-55 0.009798790348132832 56-57 0.007862502435028739 58-59 0.009417400304642632 60-61 0.010590908130766324 62-63 0.003667211956636539 64-65 0.006278266869761754 66-67 0.003579198869677262 68-69 0.0075104500871916325 70-71 0.022003271739819235 72-73 0.018717449826672894 74-75 0.020507049261511524 76-77 0.011588389782971463 78-79 0.004606018217535493 80-81 0.00636627995672103 82-83 0.0055154867827813545 84-85 0.004341978956657663 86-87 0.007217073130660709 88-89 0.02995378726180725 90-91 0.036232054131569005 92-93 0.02247267487026871 94-95 0.03303424530538194 96-97 0.035645300218507155 98-99 0.02182724556590068 100-101 0.1243624918734583 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1704292.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.53415207407403 #Duplication Level Percentage of deduplicated Percentage of total 1 78.30677047272727 43.48700099862046 2 12.08448773437158 13.422035591557474 3 3.9739446345361134 6.620689370048371 4 1.7758516364042762 3.944816593482732 5 0.9651372647430613 2.6799039816298507 6 0.6005439347347196 2.0010418919232427 7 0.40472411843135564 1.5733207518708718 8 0.2898492562335104 1.2877226139383213 9 0.2314932465447003 1.1570203041961051 >10 1.2251687046706254 12.367593122467783 >50 0.08552456565059344 3.244174822157966 >100 0.05118420728064167 5.17303898188339 >500 0.003936965516979527 1.4869614470545773 >1k 0.0012768536811825493 1.204270954179474 >5k 1.064044734318791E-4 0.3504085749894152 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5930 0.3479450704456748 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4500 0.2640392608778308 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3288 0.19292468661473502 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 1.4668847826546157E-4 0.0 26-27 0.0 0.0 0.0 7.921177826334925E-4 0.0 28-29 0.0 0.0 0.0 0.0029631072609623233 0.0 30-31 0.0 0.0 0.0 0.009828128043785925 0.0 32-33 0.0 0.0 0.0 0.024350287392066617 0.0 34-35 0.0 0.0 0.0 0.047615080044968824 0.0 36-37 0.0 0.0 0.0 0.08760235922013364 0.0 38-39 0.0 0.0 0.0 0.14792066148289143 0.0 40-41 0.0 0.0 0.0 0.2139304767023491 0.0 42-43 0.0 0.0 0.0 0.2836955169654026 0.0 44-45 0.0 0.0 0.0 0.3722660201420883 0.0 46-47 0.0 0.0 0.0 0.477030931319281 0.0 48-49 0.0 0.0 0.0 0.5891889418010529 0.0 50-51 0.0 0.0 0.0 0.71733599641376 0.0 52-53 0.0 0.0 0.0 0.8451309986786302 0.0 54-55 0.0 0.0 0.0 0.9791162547263028 0.0 56-57 0.0 0.0 0.0 1.1408549708618008 0.0 58-59 0.0 0.0 0.0 1.3194335243021738 0.0 60-61 0.0 0.0 0.0 1.5089257005254968 0.0 62-63 0.0 0.0 0.0 1.6996207222705968 0.0 64-65 0.0 0.0 0.0 1.896095270059356 0.0 66-67 0.0 0.0 0.0 2.127687039544867 0.0 68-69 0.0 0.0 0.0 2.393721263727108 0.0 70-71 0.0 0.0 0.0 2.6682340819530923 2.933769565309231E-5 72-73 0.0 0.0 0.0 2.9499346356140848 5.867539130618462E-5 74-75 0.0 0.0 0.0 3.234363594970815 5.867539130618462E-5 76-77 0.0 0.0 0.0 3.541499930763038 1.1735078261236924E-4 78-79 0.0 0.0 0.0 3.885161697643362 1.1735078261236924E-4 80-81 0.0 0.0 0.0 4.260156123481187 1.1735078261236924E-4 82-83 0.0 0.0 0.0 4.648381850058558 1.1735078261236924E-4 84-85 0.0 0.0 0.0 5.043003194288303 1.1735078261236924E-4 86-87 0.0 0.0 0.0 5.44534035247481 1.7602617391855387E-4 88-89 0.0 0.0 0.0 5.879684936618842 2.347015652247385E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3620 0.0 42.20557 1 GTATCAA 6115 0.0 39.61819 1 TATCAAC 7870 0.0 30.555216 2 ATCAACG 7970 0.0 29.933327 3 TCAACGC 8095 0.0 29.471107 4 CAACGCA 8225 0.0 29.004452 5 AACGCAG 8540 0.0 28.152239 6 ACGCAGA 9575 0.0 24.8085 7 TACATGG 6450 0.0 24.535454 2 CGCAGAG 9670 0.0 24.466518 8 GTACATG 6975 0.0 23.815216 1 GAGTACT 5255 0.0 22.687874 12-13 ACATGGG 6670 0.0 22.65744 3 AGAGTAC 8345 0.0 21.716333 10-11 GCAGAGT 10725 0.0 21.30612 9 GTACTTT 5695 0.0 20.895435 14-15 AGTACTT 5785 0.0 20.527187 12-13 TAGACAG 710 0.0 19.410488 5 TAAGGTG 720 0.0 19.1409 5 GTAAGGT 745 0.0 19.13703 4 >>END_MODULE