FastQCFastQC Report
Thu 26 May 2016
SRR938498_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938498_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1704292
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56440.331163908532106No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48180.2826980353131975No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35220.20665472818038222No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40600.043.1163641
GTATCAA64450.035.427351
ATCAACG82850.027.4308223
TATCAAC83100.027.4192052
TCAACGC84350.026.8858644
CAACGCA86050.026.4099585
AACGCAG88750.025.5802066
GTAAGGT7700.024.080454
AGGTAAG7250.023.620272
TAAGGTG9250.023.1292465
ACGCAGA99200.022.7897627
GGTAAGG8150.022.7515243
CGCAGAG101050.022.3255338
TACATGG64000.021.9261512
GTACATG67600.021.180691
GAGTACT56350.021.15555212-13
AGGACCG7350.020.699195
AGAGTAC89550.020.4722810-11
ACATGGG65150.020.4337023
GGACCGA6350.020.1945676
Produced by FastQC (version 0.11.5)