##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938498_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1704292 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.6963055626618 31.0 31.0 34.0 30.0 34.0 2 31.9316478631596 31.0 31.0 34.0 30.0 34.0 3 31.99262626357455 31.0 31.0 34.0 30.0 34.0 4 35.68757877171283 37.0 35.0 37.0 33.0 37.0 5 35.54568289940926 37.0 35.0 37.0 33.0 37.0 6 35.57856517545115 37.0 35.0 37.0 33.0 37.0 7 35.514202965219575 37.0 35.0 37.0 33.0 37.0 8 35.498614674011264 37.0 35.0 37.0 33.0 37.0 9 37.06081117554973 39.0 37.0 39.0 33.0 39.0 10-11 36.960350104324846 39.0 37.0 39.0 33.0 39.0 12-13 36.84172635909809 39.0 37.0 39.0 32.5 39.0 14-15 37.854602086966324 40.0 37.0 41.0 32.5 41.0 16-17 37.76619030072311 40.0 37.0 41.0 32.0 41.0 18-19 37.875267266407405 39.5 37.0 41.0 32.5 41.0 20-21 37.92602001300247 40.0 37.0 41.0 32.5 41.0 22-23 37.82365873923013 40.0 37.0 41.0 32.0 41.0 24-25 37.71331673210929 40.0 37.0 41.0 32.0 41.0 26-27 37.50588074109366 39.0 36.5 41.0 32.0 41.0 28-29 37.37663352289397 39.0 36.0 41.0 31.5 41.0 30-31 37.121407012413364 39.0 36.0 40.5 31.0 41.0 32-33 36.90563442180096 39.0 35.5 40.0 30.5 41.0 34-35 36.7445757534507 39.0 35.0 40.0 30.0 41.0 36-37 36.74625885705032 39.0 35.0 40.0 30.0 41.0 38-39 36.61632660365712 39.0 35.0 40.0 30.0 41.0 40-41 36.43361612916097 39.0 35.0 40.0 30.0 41.0 42-43 36.202844054892 38.5 35.0 40.0 30.0 41.0 44-45 35.919774604351836 38.0 35.0 40.0 29.0 41.0 46-47 35.53186044410231 38.0 34.5 40.0 27.5 41.0 48-49 35.13530515897511 38.0 34.0 40.0 26.5 41.0 50-51 34.7436032088398 38.0 33.5 40.0 26.0 41.0 52-53 34.22475139236704 37.5 33.0 40.0 24.0 41.0 54-55 33.87633046449787 37.0 33.0 40.0 23.5 41.0 56-57 33.50581854517888 37.0 32.0 39.5 23.0 41.0 58-59 33.022534577408095 36.0 31.5 39.0 22.0 40.0 60-61 32.62011644718159 36.0 31.0 39.0 20.5 40.0 62-63 32.12866046428664 35.0 31.0 38.5 19.0 40.0 64-65 31.58206017513431 35.0 30.0 38.0 17.5 40.0 66-67 31.063443060226767 34.5 29.0 38.0 14.0 40.0 68-69 30.491277022951465 34.0 29.0 37.0 10.0 39.0 70-71 29.887207415161253 34.0 28.0 36.5 8.0 39.0 72-73 29.421861981397555 34.0 28.0 36.0 7.0 39.0 74-75 28.880344448017127 33.0 27.0 35.5 6.0 38.0 76-77 27.67553300725463 31.5 26.0 34.0 2.0 36.0 78-79 28.38013145634668 33.0 27.0 35.0 2.0 37.0 80-81 28.10212686558407 33.0 26.0 35.0 2.0 36.0 82-83 27.610580229209546 33.0 26.0 35.0 2.0 36.0 84-85 27.058487336677047 32.0 25.5 35.0 2.0 35.5 86-87 26.444871536098276 32.0 24.5 34.0 2.0 35.0 88-89 25.75720445792153 31.0 23.0 34.0 2.0 35.0 90-91 25.16290283589901 31.0 20.0 34.0 2.0 35.0 92-93 24.364863826151854 31.0 18.0 34.0 2.0 35.0 94-95 23.525092237715135 30.5 6.0 34.0 2.0 35.0 96-97 22.441118364693374 30.0 2.0 34.0 2.0 35.0 98-99 21.122819329082105 29.0 2.0 34.0 2.0 35.0 100-101 17.564189117827226 22.5 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.35812857840540957 1101 2 0.40632546420617643 1101 3 0.35429069995571894 1101 4 0.19024527191661633 1101 5 0.1224548242319301 1101 6 0.21605451197218173 1101 7 0.23437940637770538 1101 8 0.22982965937463007 1101 9 0.18773543591930775 1101 10-11 0.2941243650498677 1101 12-13 0.2562349623949274 1101 14-15 0.4337546777030923 1101 16-17 0.4091511975701678 1101 18-19 0.5720931423648992 1101 20-21 0.4644034406015649 1101 22-23 0.43252172289535906 1101 24-25 0.5172669346909871 1101 26-27 0.5697361722879251 1101 28-29 0.408812055249804 1101 30-31 0.4617465926825801 1101 32-33 0.4063348782064935 1101 34-35 0.1836031970341665 1101 36-37 0.4529377243038084 1101 38-39 0.4620029330312647 1101 40-41 0.4876036463690241 1101 42-43 0.5392021288269646 1101 44-45 0.5132939244781625 1101 46-47 0.5643935075469955 1101 48-49 0.6508736579141825 1101 50-51 0.6557060278723696 1101 52-53 0.700905436744641 1101 54-55 0.6541834956962873 1101 56-57 0.7403824232973335 1101 58-59 0.7738731755150638 1101 60-61 0.5412746369276746 1101 62-63 0.5039694429590824 1101 64-65 0.6170180326613384 1101 66-67 0.6684885876740871 1101 68-69 0.5416219919943082 1101 70-71 0.5427923539652078 1101 72-73 0.3782964160492277 1101 74-75 0.34903437403415083 1101 76-77 0.47869557775610616 1101 78-79 0.625737908439735 1101 80-81 0.7217402151329679 1101 82-83 0.5670166861405725 1101 84-85 0.7676260507767729 1101 86-87 0.9260444627268534 1101 88-89 0.7688362462011504 1101 90-91 0.8717667442718806 1101 92-93 0.53255868775247 1101 94-95 0.5937058593695177 1101 96-97 0.6136549481091969 1101 98-99 0.6427002207005614 1101 100-101 0.5302629304385995 1102 1 0.32784703213221533 1102 2 0.28703099429916534 1102 3 0.2777924803386327 1102 4 0.2757776255928519 1102 5 0.1530723635529725 1102 6 0.2447604859078325 1102 7 0.22650119901814492 1102 8 0.34323388078851735 1102 9 0.28780087160238566 1102 10-11 0.32313850544864664 1102 12-13 0.37810226795537005 1102 14-15 0.4477597459438982 1102 16-17 0.40021903670969294 1102 18-19 0.39327587937395236 1102 20-21 0.37251469992908426 1102 22-23 0.32512424139140705 1102 24-25 0.32480168249052355 1102 26-27 0.39339063593136103 1102 28-29 0.41634044268712245 1102 30-31 0.43116966980508664 1102 32-33 0.3730943808123399 1102 34-35 0.25071383311532713 1102 36-37 0.27409505443115023 1102 38-39 0.30054842189251474 1102 40-41 0.36437024561814724 1102 42-43 0.5286653591927077 1102 44-45 0.5844812416393523 1102 46-47 0.403202398365508 1102 48-49 0.5744922753857509 1102 50-51 0.38876456359945877 1102 52-53 0.3042735942133845 1102 54-55 0.3113176270937714 1102 56-57 0.31211555632534527 1102 58-59 0.44523049409696824 1102 60-61 0.465015622237658 1102 62-63 0.36298769339627057 1102 64-65 0.4008510980413682 1102 66-67 0.2579437984526898 1102 68-69 0.30652883169970124 1102 70-71 0.293353638647762 1102 72-73 0.20890804438146304 1102 74-75 0.22573996220000936 1102 76-77 0.19413746564988443 1102 78-79 0.2758481604794589 1102 80-81 0.1959306257116289 1102 82-83 0.18191023476294887 1102 84-85 0.18492250276623423 1102 86-87 -0.013942062984046544 1102 88-89 -0.04490763238229789 1102 90-91 -0.26425063887461064 1102 92-93 -0.34033673233735584 1102 94-95 -0.29019264169359715 1102 96-97 -0.2268577593068848 1102 98-99 -0.2979512048329056 1102 100-101 -0.028260006790500114 1103 1 0.2919022663169457 1103 2 0.22921684390836816 1103 3 0.20944271402094472 1103 4 0.2712300034326347 1103 5 0.19141562030508652 1103 6 0.1467553972221438 1103 7 0.33771731155398044 1103 8 0.2341853830127647 1103 9 0.43061341392548513 1103 10-11 0.3587080554686253 1103 12-13 0.39555845609223894 1103 14-15 0.6080489763264012 1103 16-17 0.5813793726530108 1103 18-19 0.45229918913013023 1103 20-21 0.47404224661597993 1103 22-23 0.5005298121949409 1103 24-25 0.5059803339384032 1103 26-27 0.42983933017031717 1103 28-29 0.4347981024495695 1103 30-31 0.5900190326775743 1103 32-33 0.5432891065519385 1103 34-35 0.338737139846927 1103 36-37 0.4656896829137409 1103 38-39 0.6823675446907416 1103 40-41 0.6109402893165949 1103 42-43 0.6671245245232384 1103 44-45 0.7492453362602731 1103 46-47 0.8387901294643285 1103 48-49 0.9026101707763274 1103 50-51 0.8372198608298476 1103 52-53 0.9142412278602805 1103 54-55 0.9246685881200563 1103 56-57 0.8536715878226389 1103 58-59 0.9618493780206947 1103 60-61 1.1017983307882417 1103 62-63 0.8375516583635871 1103 64-65 0.9420710652347672 1103 66-67 1.0175213790652684 1103 68-69 0.9431440121947965 1103 70-71 0.6893736627032432 1103 72-73 0.7871097037997821 1103 74-75 0.856996933433912 1103 76-77 0.7304182957690415 1103 78-79 1.0167301478024413 1103 80-81 0.9868273830607706 1103 82-83 0.9302866240947871 1103 84-85 0.8668270531105158 1103 86-87 1.1377272860358723 1103 88-89 0.8675377025578719 1103 90-91 1.0322765398218685 1103 92-93 1.0091346889222734 1103 94-95 0.9542635575333875 1103 96-97 0.68698543510531 1103 98-99 0.5391886728598507 1103 100-101 0.20700029685703925 1104 1 0.28198984593478116 1104 2 0.28429429034728315 1104 3 0.3694854181321219 1104 4 0.22597812155655106 1104 5 0.25663710264440454 1104 6 0.1589043549523126 1104 7 0.21735324091125108 1104 8 0.22347678428664608 1104 9 0.2755251105091574 1104 10-11 0.37205309021094735 1104 12-13 0.2523114960459196 1104 14-15 0.46825996358754196 1104 16-17 0.41306908023842226 1104 18-19 0.4220444120600675 1104 20-21 0.3845898001770678 1104 22-23 0.43574079945608446 1104 24-25 0.4921503542047674 1104 26-27 0.42556891905277183 1104 28-29 0.5814577723974637 1104 30-31 0.5285866354569606 1104 32-33 0.48080358252182975 1104 34-35 0.5513674235759396 1104 36-37 0.557291810881317 1104 38-39 0.5421478718476607 1104 40-41 0.5283729760566018 1104 42-43 0.6092622633767419 1104 44-45 0.3801859124038742 1104 46-47 0.3213303002807706 1104 48-49 0.5388254284485896 1104 50-51 0.5758164155490135 1104 52-53 0.6143335121683222 1104 54-55 0.5528677774657424 1104 56-57 0.49711397346248276 1104 58-59 0.6346381736199973 1104 60-61 0.34055231167996425 1104 62-63 0.3542750225789817 1104 64-65 0.5029696610656877 1104 66-67 0.4274392279361443 1104 68-69 0.3631443740940412 1104 70-71 0.19971493369339655 1104 72-73 0.07417904369555472 1104 74-75 0.10275547425614562 1104 76-77 0.18452223323285466 1104 78-79 -0.07818335827746736 1104 80-81 0.27810561120205435 1104 82-83 0.17693905605772997 1104 84-85 0.2295984775459523 1104 86-87 0.254242558184103 1104 88-89 0.2081952734901229 1104 90-91 0.15482467242175346 1104 92-93 0.10673348741677202 1104 94-95 0.21854410182985617 1104 96-97 -0.043808571843154454 1104 98-99 -0.10939992007587662 1104 100-101 -0.018828452419160868 1105 1 0.23053474615651126 1105 2 0.26313298214328995 1105 3 0.17077422079287885 1105 4 0.13052357610200715 1105 5 0.05810606051580436 1105 6 0.21598584053989356 1105 7 0.2601331743924007 1105 8 0.19502611909817347 1105 9 0.22594085330295144 1105 10-11 0.232120994867266 1105 12-13 0.3848780148929194 1105 14-15 0.47386556225717413 1105 16-17 0.4063825503049401 1105 18-19 0.47110206172747127 1105 20-21 0.4706139132591076 1105 22-23 0.46667899236074106 1105 24-25 0.4076090570872495 1105 26-27 0.3177529545294888 1105 28-29 0.33554590986974375 1105 30-31 0.4816769902241447 1105 32-33 0.5378712100162915 1105 34-35 0.4656966918686223 1105 36-37 0.4790005137638005 1105 38-39 0.5941365082112995 1105 40-41 0.5504766345931955 1105 42-43 0.7873456890973642 1105 44-45 0.6587405132907946 1105 46-47 0.5103339033849892 1105 48-49 0.547597601397598 1105 50-51 0.5475874798505629 1105 52-53 0.6328548536317413 1105 54-55 0.7381227663792629 1105 56-57 0.761415525568907 1105 58-59 0.8003116769459382 1105 60-61 0.7978347396178833 1105 62-63 0.8646270181443967 1105 64-65 0.8511196056333183 1105 66-67 0.8555087955636402 1105 68-69 0.7360864472647748 1105 70-71 0.7009968073075896 1105 72-73 0.6517982232964421 1105 74-75 0.6670015939900686 1105 76-77 0.5945277764701018 1105 78-79 0.6874000674431571 1105 80-81 0.7590091588738943 1105 82-83 0.8272300760133824 1105 84-85 0.7779216482776583 1105 86-87 0.9185787555233524 1105 88-89 0.7728224907850212 1105 90-91 0.7244962356146551 1105 92-93 0.7412886426274135 1105 94-95 0.8358112858653328 1105 96-97 0.6846975257178194 1105 98-99 0.4492752462212408 1105 100-101 -0.40889219964754275 1106 1 0.12621263956451045 1106 2 0.06285746986849006 1106 3 0.11154841899986678 1106 4 0.04732210603922482 1106 5 -0.09528661232675262 1106 6 -0.0680943306686359 1106 7 -0.057317845301810166 1106 8 0.06741245627888759 1106 9 -0.10678628247741528 1106 10-11 -0.015174303745460804 1106 12-13 -0.05865846465032121 1106 14-15 -0.10873505325692179 1106 16-17 -0.2091147350339 1106 18-19 -0.17183798291721786 1106 20-21 -0.12683843855500498 1106 22-23 0.10062598270349099 1106 24-25 -0.04550421294342044 1106 26-27 -0.18719331870946831 1106 28-29 0.07816384916740304 1106 30-31 0.03143711526911375 1106 32-33 0.09091090220944409 1106 34-35 0.0405710119583631 1106 36-37 -0.4856933289788188 1106 38-39 -0.38287461934727673 1106 40-41 -0.03541428595146101 1106 42-43 -0.007985292899100216 1106 44-45 -0.058870765407363024 1106 46-47 -0.283338776080825 1106 48-49 -0.13532564857656837 1106 50-51 -0.14661256969931458 1106 52-53 -0.18042348721915147 1106 54-55 -0.490351047504916 1106 56-57 -0.04912792332686422 1106 58-59 0.019577367637658938 1106 60-61 -0.38619482348414635 1106 62-63 -0.38095193112424397 1106 64-65 -0.6627593407718884 1106 66-67 -0.504851268975699 1106 68-69 -1.306301576852821 1106 70-71 -0.7607252875816748 1106 72-73 -0.512295294010535 1106 74-75 -0.20077803602919175 1106 76-77 -0.18821716240800157 1106 78-79 -0.49602366122269714 1106 80-81 -1.255646494356622 1106 82-83 -0.6941608750330772 1106 84-85 -0.6951096154559373 1106 86-87 -0.7557848459810721 1106 88-89 -0.7491469053164721 1106 90-91 -0.787420012637714 1106 92-93 -0.9868619454350487 1106 94-95 -0.8735447399852632 1106 96-97 -0.8265184258577527 1106 98-99 -0.916851683640779 1106 100-101 -1.034705321900045 1107 1 0.1546012934760057 1107 2 0.20953293279097807 1107 3 0.20451174480170664 1107 4 0.20319248023158565 1107 5 0.15547277072164434 1107 6 0.2132999359630645 1107 7 0.1243413604695931 1107 8 0.12738231227925212 1107 9 0.21838407738497523 1107 10-11 0.2249011433750141 1107 12-13 0.2680752734669909 1107 14-15 0.3594300411691407 1107 16-17 0.3284064932935351 1107 18-19 0.14504893562638443 1107 20-21 0.14115550740991267 1107 22-23 0.31310062362068436 1107 24-25 0.3650300844932062 1107 26-27 0.44786450735959704 1107 28-29 0.32484114464318736 1107 30-31 0.3176859958344096 1107 32-33 0.3802156405013051 1107 34-35 0.4615715920666261 1107 36-37 0.27102560751539073 1107 38-39 0.33353895969240455 1107 40-41 0.3550957616979247 1107 42-43 0.42183963216900366 1107 44-45 0.29327583044463523 1107 46-47 0.3991844335070951 1107 48-49 0.5093909350393773 1107 50-51 0.36955851146808527 1107 52-53 0.4104077679686995 1107 54-55 0.34844149214058007 1107 56-57 0.2548684848686733 1107 58-59 0.053486062622376096 1107 60-61 0.1530393915476651 1107 62-63 0.09553280505867434 1107 64-65 0.11985657798771499 1107 66-67 0.1441677592363071 1107 68-69 0.17202172099807456 1107 70-71 0.10077484054684405 1107 72-73 0.07098974703715655 1107 74-75 0.2738131618868671 1107 76-77 0.1288513591631002 1107 78-79 0.4598323830037252 1107 80-81 0.4859640916728942 1107 82-83 0.3475228028352966 1107 84-85 0.46605909090263253 1107 86-87 0.5817258076125782 1107 88-89 0.3476541175486858 1107 90-91 0.5308210426245843 1107 92-93 0.40196170255369523 1107 94-95 0.15251401768179562 1107 96-97 0.2076327069155397 1107 98-99 0.14133759147624403 1107 100-101 -0.5702606110844677 1108 1 0.1495910562335041 1108 2 0.14356817324404858 1108 3 0.11783554736319957 1108 4 0.0072570121574742075 1108 5 0.055914526969672806 1108 6 0.028111012837896965 1108 7 0.03971114885895588 1108 8 -0.025360446689049354 1108 9 0.10512586104347577 1108 10-11 0.04599703001909461 1108 12-13 0.16590498975832446 1108 14-15 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64-65 -0.3554845829313784 2316 66-67 -0.4639859450742314 2316 68-69 -0.5322429987695401 2316 70-71 -0.7107276189961276 2316 72-73 -0.4737481084487243 2316 74-75 -0.3229208390499245 2316 76-77 -0.42160259637813624 2316 78-79 -0.35383247487597913 2316 80-81 -0.5985235698821469 2316 82-83 -0.239311468216151 2316 84-85 -0.1252504005079409 2316 86-87 0.0715196762505812 2316 88-89 0.4075468122339636 2316 90-91 0.3240750263066232 2316 92-93 0.30014893514666596 2316 94-95 0.18831578055482012 2316 96-97 0.3552182447658403 2316 98-99 0.4809049451857348 2316 100-101 0.41691588542292735 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 12.0 4 25.0 5 71.0 6 144.0 7 361.0 8 842.0 9 1451.0 10 2406.0 11 3785.0 12 5240.0 13 7142.0 14 9248.0 15 12167.0 16 15174.0 17 19002.0 18 18067.0 19 16131.0 20 16247.0 21 17496.0 22 18641.0 23 20981.0 24 24211.0 25 28694.0 26 33912.0 27 40827.0 28 48747.0 29 58348.0 30 68372.0 31 81122.0 32 97505.0 33 119254.0 34 148599.0 35 190039.0 36 237999.0 37 240661.0 38 98181.0 39 3186.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.36111437087802 19.080608820146924 15.266094773159338 23.292182035815713 2 16.308300641202123 20.479372405003996 38.441015333553665 24.771311620240223 3 19.694861809435906 23.751127310877283 29.72380663471959 26.83020424496722 4 13.866285824260164 16.750826736263505 35.21638310805895 34.166504331417386 5 14.356707184578685 37.030020213931756 33.57662090245046 15.036651699039105 6 33.592952381399435 35.339894806758466 16.47270538147219 14.594447430369915 7 29.64028464605831 32.20170017813849 19.699793227921035 18.45822194788217 8 27.353469945291064 34.92500111483243 18.794960018588363 18.926568921288137 9 26.577898623005918 16.49817050129907 19.22375977825396 37.70017109744105 10-11 25.685709960499725 26.393423192739274 26.56581149239684 21.355055354364158 12-13 26.44652442187137 23.3378435150784 27.121086058022918 23.094546005027308 14-15 22.954399832892484 24.75608639834019 25.22249708383305 27.06701668493427 16-17 22.791516946626516 28.083773203183494 25.995310662726812 23.129399187463182 18-19 22.897015300195037 26.695542782574815 28.253814487188816 22.153627430041333 20-21 23.30454523051214 25.71903758276164 28.519848711371054 22.456568475355162 22-23 23.078087557765922 25.567889774756907 28.659320116505853 22.694702550971314 24-25 23.102961471300627 25.66048109239753 28.720409930715935 22.51614750558591 26-27 22.55272715963281 26.09715299891176 28.45636752905374 22.89375231240169 28-29 22.678596156057765 26.42205678369669 28.406751894628385 22.49259516561716 30-31 22.72324812884177 25.99352106329197 28.286467342450706 22.99676346541555 32-33 22.49365132266067 25.851057213200555 28.386508884627755 23.268782579511022 34-35 23.132434319783577 26.033044029364643 28.210540523094433 22.623981127757336 36-37 23.267009390733307 25.586572445419243 28.278138681495157 22.868279482352293 38-39 22.6368600021711 25.950135106985368 28.347293899501523 23.065710991342 40-41 23.07755049349247 25.775152859439732 28.364932108110647 22.782364538957154 42-43 22.88938006883821 25.849681332827938 28.08454684249005 23.176391755843795 44-45 22.80149176314857 26.066161197729027 28.140453631185263 22.991893407937138 46-47 23.26467530211959 26.2713197034311 27.628393491256194 22.835611503193114 48-49 22.974642842893118 26.40328065847871 27.554491835906052 23.067584662722115 50-51 23.156272516681415 26.392865776521862 27.46771093216421 22.983150774632517 52-53 23.466694674386783 26.1403562300357 27.346810288377814 23.046138807199707 54-55 23.183820612899666 26.506050606351494 27.245331199113764 23.064797581635073 56-57 23.02874155367742 26.60488930300676 27.20616537541689 23.160203767898928 58-59 23.07899702633117 26.227137133777546 27.340414670725437 23.353451169165847 60-61 22.819182393627383 26.44910613908884 27.521809642948508 23.209901824335265 62-63 22.856177227845933 26.535153600439358 27.664948260039946 22.94372091167476 64-65 22.77048181884325 26.56575281700554 27.283147488810606 23.38061787534061 66-67 22.592907375293485 26.60196333784449 27.270569617932143 23.534559668929873 68-69 23.015047129914507 26.34378712164708 27.343858412812228 23.29730733562619 70-71 23.064481633570313 26.275170650091255 26.994296456914796 23.666051259423636 72-73 22.791370258148252 26.459168968697856 27.029845824541805 23.71961494861209 74-75 22.661228240231136 26.717047313488536 26.958056483278686 23.663667963001643 76-77 22.878796591194465 26.46465511778498 27.07402839419536 23.58251989682519 78-79 22.757719921234155 26.639566459268714 27.19689466359051 23.405818955906618 80-81 22.840212278508606 26.45568881231002 27.050166038783164 23.653932870398215 82-83 22.86676653294443 26.46327273879453 27.145320689863574 23.524640038397468 84-85 22.681464951577173 26.58577466991236 27.02694773197966 23.705812646530806 86-87 22.915578092604072 26.588882946248376 26.743349221991657 23.752189739155895 88-89 22.7798765645431 26.62924740280639 26.62211834067118 23.968757691979338 90-91 22.514510935130897 26.537672100981556 26.897599622586597 24.05021734130095 92-93 22.683656486289923 26.29073175082315 26.800684513742056 24.22492724914487 94-95 22.601496691881437 26.031249339901848 27.094124715717726 24.273129252498986 96-97 22.60710019175118 25.97841215003063 27.04782983197715 24.366657826241042 98-99 22.384022920961584 26.129861000890987 26.770420435705983 24.71569564244145 100-101 22.514569099661326 25.803060743112095 27.089870749848032 24.592499407378547 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 448.0 1 320.5 2 256.0 3 333.5 4 314.5 5 306.5 6 561.0 7 1283.0 8 1968.0 9 2109.0 10 1776.0 11 1463.5 12 1473.5 13 1574.0 14 1680.0 15 1793.5 16 1895.5 17 2031.5 18 2179.5 19 2371.0 20 2609.5 21 2958.0 22 3501.0 23 4155.0 24 5121.5 25 6265.5 26 7838.5 27 9984.5 28 11829.5 29 13778.5 30 16254.0 31 19670.5 32 24193.0 33 26703.0 34 27626.5 35 29371.0 36 32351.5 37 35707.0 38 38513.5 39 40692.0 40 42728.0 41 45614.0 42 48209.5 43 51269.5 44 55003.5 45 58048.5 46 59338.0 47 61988.5 48 65421.0 49 66903.0 50 66584.5 51 65469.5 52 64613.0 53 64702.5 54 65986.5 55 63169.5 56 58479.5 57 54121.5 58 49663.0 59 44512.5 60 36344.0 61 29812.5 62 25205.0 63 18936.0 64 13365.0 65 10126.0 66 7819.5 67 6445.0 68 5192.0 69 4093.5 70 3277.0 71 2087.0 72 1422.0 73 1076.0 74 724.0 75 518.0 76 367.0 77 242.5 78 152.0 79 88.0 80 56.0 81 35.5 82 19.0 83 10.0 84 8.0 85 4.5 86 2.0 87 1.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 7.041046956742155E-4 2 0.04535607747968071 3 8.801308695927692E-4 4 0.0 5 0.08836513930711404 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.3470156522473852E-4 26-27 0.009710777261173555 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011852429043849293 36-37 3.2271465218401544E-4 38-39 0.005104759043638062 40-41 0.006571643826292677 42-43 3.5205234783710775E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.9337695653092314E-5 68-69 7.921177826334923E-4 70-71 2.9337695653092314E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0012615209130829694 82-83 7.627800869804001E-4 84-85 0.005016745956678785 86-87 0.002141651782675739 88-89 2.9337695653092314E-5 90-91 0.003520523478371077 92-93 9.681439565520463E-4 94-95 0.0 96-97 0.0 98-99 2.9337695653092314E-5 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1704292.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.657632264567766 #Duplication Level Percentage of deduplicated Percentage of total 1 76.92124544222898 41.27411901271682 2 12.759394658790566 13.692778130397484 3 4.281618902303184 6.892245976704197 4 1.9239280889528656 4.129337036020219 5 1.0022685295618348 2.6889678094789 6 0.6421351484859352 2.0673270996967164 7 0.43303682715594655 1.6265011579984279 8 0.33079277498192017 1.4199645660604625 9 0.22659747646116196 1.094281565762887 >10 1.323404127804682 12.95102472609227 >50 0.09487840776446822 3.4812095080770655 >100 0.05522163772057144 5.568821738977892 >500 0.004163263118684637 1.5833119822591575 >1k 0.0012051551133034476 1.1983155031511776 >5k 1.0955955575485888E-4 0.331794186606419 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5644 0.331163908532106 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4818 0.2826980353131975 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3522 0.20665472818038222 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 5.867539130618462E-5 14-15 0.0 0.0 0.0 0.0 5.867539130618462E-5 16-17 0.0 0.0 0.0 0.0 5.867539130618462E-5 18-19 0.0 0.0 0.0 5.867539130618462E-5 5.867539130618462E-5 20-21 0.0 0.0 0.0 5.867539130618462E-5 5.867539130618462E-5 22-23 0.0 0.0 0.0 5.867539130618462E-5 5.867539130618462E-5 24-25 0.0 0.0 0.0 2.0536386957164618E-4 5.867539130618462E-5 26-27 0.0 0.0 0.0 8.50793173939677E-4 5.867539130618462E-5 28-29 0.0 0.0 0.0 0.0028750941740030463 5.867539130618462E-5 30-31 0.0 0.0 0.0 0.009681439565520462 5.867539130618462E-5 32-33 0.0 0.0 0.0 0.024174261218148065 8.801308695927694E-5 34-35 0.0 0.0 0.0 0.04743905387105027 1.1735078261236924E-4 36-37 0.0 0.0 0.0 0.08795441156797074 1.1735078261236924E-4 38-39 0.0 0.0 0.0 0.14903549391770893 1.1735078261236924E-4 40-41 0.0 0.0 0.0 0.21489862065890117 1.1735078261236924E-4 42-43 0.0 0.0 0.0 0.28478101170456704 1.1735078261236924E-4 44-45 0.0 0.0 0.0 0.37402628188127385 1.1735078261236924E-4 46-47 0.0 0.0 0.0 0.4794366223628345 1.1735078261236924E-4 48-49 0.0 0.0 0.0 0.5915359574533002 1.1735078261236924E-4 50-51 0.0 0.0 0.0 0.7198297005442729 1.1735078261236924E-4 52-53 0.0 0.0 0.0 0.8477420535917554 1.1735078261236924E-4 54-55 0.0 0.0 0.0 0.9812872442046315 1.1735078261236924E-4 56-57 0.0 0.0 0.0 1.141940465600965 1.1735078261236924E-4 58-59 0.0 0.0 0.0 1.3200202782152353 1.1735078261236924E-4 60-61 0.0 0.0 0.0 1.5094244413515994 1.1735078261236924E-4 62-63 0.0 0.0 0.0 1.7000607877053933 1.1735078261236924E-4 64-65 0.0 0.0 0.0 1.8958605684941312 1.1735078261236924E-4 66-67 0.0 0.0 0.0 2.126806908675274 1.1735078261236924E-4 68-69 0.0 0.0 0.0 2.392166365857494 1.1735078261236924E-4 70-71 0.0 0.0 0.0 2.666796534866091 1.1735078261236924E-4 72-73 0.0 0.0 0.0 2.947998347700981 1.1735078261236924E-4 74-75 0.0 0.0 0.0 3.2345689588403865 1.1735078261236924E-4 76-77 0.0 0.0 0.0 3.544228336458776 1.1735078261236924E-4 78-79 0.0 0.0 0.0 3.8934055901218807 1.1735078261236924E-4 80-81 0.0 0.0 0.0 4.274472918959896 1.4668847826546157E-4 82-83 0.0 0.0 0.0 4.668243470015701 2.347015652247385E-4 84-85 0.0 0.0 0.0 5.067588183245594 2.347015652247385E-4 86-87 0.0 0.0 0.0 5.477934532345396 2.347015652247385E-4 88-89 0.0 0.0 0.0 5.9229287000115 2.347015652247385E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4060 0.0 43.116364 1 GTATCAA 6445 0.0 35.42735 1 ATCAACG 8285 0.0 27.430822 3 TATCAAC 8310 0.0 27.419205 2 TCAACGC 8435 0.0 26.885864 4 CAACGCA 8605 0.0 26.409958 5 AACGCAG 8875 0.0 25.580206 6 GTAAGGT 770 0.0 24.08045 4 AGGTAAG 725 0.0 23.62027 2 TAAGGTG 925 0.0 23.129246 5 ACGCAGA 9920 0.0 22.789762 7 GGTAAGG 815 0.0 22.751524 3 CGCAGAG 10105 0.0 22.325533 8 TACATGG 6400 0.0 21.926151 2 GTACATG 6760 0.0 21.18069 1 GAGTACT 5635 0.0 21.155552 12-13 AGGACCG 735 0.0 20.69919 5 AGAGTAC 8955 0.0 20.47228 10-11 ACATGGG 6515 0.0 20.433702 3 GGACCGA 635 0.0 20.194567 6 >>END_MODULE