FastQCFastQC Report
Thu 26 May 2016
SRR938497_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938497_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1704667
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60970.35766516275612775No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46490.2727218864446839No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33290.1952874080392241No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36500.045.4458541
GTATCAA61750.039.1362231
ATCAACG79650.030.3160483
TCAACGC80000.030.0645794
TATCAAC80500.030.0549872
CAACGCA81850.029.326985
AACGCAG84450.028.531626
ACGCAGA96050.024.7780577
CGCAGAG96300.024.7137328
TACATGG68700.024.1470072
GAGTACT51700.023.29103512-13
GTACATG73600.023.1205581
ACATGGG69350.022.7554933
GTGGTAT21600.021.8468761
GTACTTT54850.021.69682714-15
AGAGTAC85200.021.4085210-11
GCAGAGT108600.021.2135479
AGTACTT54750.021.16932912-13
TATAGCG1150.003709080220.6664925
TGGTATC21050.020.548682
Produced by FastQC (version 0.11.5)