##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938497_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1704667 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.143757695784572 31.0 31.0 34.0 28.0 34.0 2 31.293513630521385 31.0 31.0 34.0 28.0 34.0 3 31.344134660904448 31.0 31.0 34.0 28.0 34.0 4 35.02960988861754 37.0 35.0 37.0 32.0 37.0 5 35.02749627933198 37.0 35.0 37.0 32.0 37.0 6 35.0292256493497 37.0 35.0 37.0 32.0 37.0 7 34.95111889888172 37.0 35.0 37.0 32.0 37.0 8 34.908025438399406 37.0 35.0 37.0 32.0 37.0 9 36.399722643777345 39.0 37.0 39.0 32.0 39.0 10-11 36.25433911725868 39.0 35.0 39.0 32.0 39.0 12-13 36.079301998572156 38.0 35.0 39.0 30.5 39.0 14-15 37.0668532329188 39.0 36.0 41.0 31.0 41.0 16-17 36.89133508186643 39.0 36.0 41.0 31.0 41.0 18-19 36.75105313823756 39.0 36.0 40.0 30.0 41.0 20-21 36.72432709731578 39.0 36.0 40.5 30.0 41.0 22-23 36.8220740473066 39.0 36.0 41.0 30.0 41.0 24-25 36.72410388656553 39.0 36.0 41.0 30.0 41.0 26-27 36.52703137914912 39.0 36.0 41.0 30.0 41.0 28-29 36.33247666553057 39.0 35.0 40.0 29.5 41.0 30-31 36.05752941776898 38.5 35.0 40.0 29.0 41.0 32-33 35.75748899931776 38.0 35.0 40.0 27.5 41.0 34-35 35.48918351795395 38.0 34.0 40.0 27.0 41.0 36-37 35.192750548934185 38.0 34.0 40.0 26.0 41.0 38-39 34.860319933453276 38.0 33.5 40.0 25.0 41.0 40-41 34.748131159927425 38.0 33.0 40.0 25.0 41.0 42-43 34.500207958504504 38.0 33.0 40.0 24.0 41.0 44-45 34.17808492802406 38.0 33.0 40.0 23.5 41.0 46-47 33.766342048036364 38.0 33.0 40.0 22.5 41.0 48-49 33.34469547424805 37.5 32.0 40.0 19.5 41.0 50-51 32.55817617165112 36.5 30.5 39.5 18.0 40.5 52-53 32.58221723069667 37.0 31.0 39.0 18.0 40.0 54-55 32.63553556207752 37.0 31.0 39.5 16.0 41.0 56-57 32.28363809471293 36.0 31.0 39.0 12.0 41.0 58-59 31.8191171648187 36.0 30.5 39.0 8.0 41.0 60-61 31.30766067507613 35.0 29.5 39.0 7.0 40.0 62-63 30.690378824720604 35.0 28.5 38.5 2.0 40.0 64-65 30.165149850381336 34.5 28.0 38.0 2.0 40.0 66-67 29.576755166844904 34.0 28.0 38.0 2.0 40.0 68-69 28.932207287405692 34.0 26.5 37.0 2.0 39.5 70-71 28.26150092657393 34.0 26.0 36.5 2.0 39.0 72-73 27.593790752094105 33.0 25.0 36.0 2.0 39.0 74-75 26.875395605124048 32.5 23.5 35.0 2.0 38.0 76-77 26.12200506022584 32.0 21.0 35.0 2.0 37.0 78-79 25.308062806401487 31.0 19.0 35.0 2.0 36.5 80-81 24.54985108528528 31.0 14.5 34.5 2.0 36.0 82-83 23.810903243859357 30.5 7.0 34.0 2.0 36.0 84-85 23.046316963958354 30.0 2.0 34.0 2.0 35.0 86-87 22.30320085975736 29.5 2.0 34.0 2.0 35.0 88-89 21.505341219135467 29.0 2.0 34.0 2.0 35.0 90-91 20.650191210365428 28.0 2.0 34.0 2.0 35.0 92-93 19.757001220766284 26.5 2.0 33.5 2.0 35.0 94-95 18.825966889720984 25.0 2.0 33.0 2.0 35.0 96-97 17.721616010634335 22.0 2.0 33.0 2.0 35.0 98-99 16.458209433279343 10.0 2.0 32.0 2.0 34.0 100-101 13.76629335817494 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7262406780838866 1101 2 0.6409276488427409 1101 3 0.6767163712619357 1101 4 0.4323986353822349 1101 5 0.5192798616882115 1101 6 0.4820925065363397 1101 7 0.5068010407431913 1101 8 0.5518212831674703 1101 9 0.7340457767931454 1101 10-11 0.6497691388503384 1101 12-13 0.6819864686327577 1101 14-15 0.8330894951270693 1101 16-17 1.0713607653068848 1101 18-19 0.9736723652110157 1101 20-21 1.0486721058539317 1101 22-23 1.0287705320442413 1101 24-25 1.1124268191178643 1101 26-27 1.110474732817778 1101 28-29 1.2780134425856815 1101 30-31 1.1434278217447584 1101 32-33 1.1949855199050674 1101 34-35 1.3490109883745376 1101 36-37 1.316009005557575 1101 38-39 1.3285969111856701 1101 40-41 1.3598859021767211 1101 42-43 1.5604758689530982 1101 44-45 1.7617012815207147 1101 46-47 1.9145497230477204 1101 48-49 1.8962678022773432 1101 50-51 1.7652165480786195 1101 52-53 2.143427701627317 1101 54-55 2.1113023997887765 1101 56-57 2.1951038508735436 1101 58-59 2.2731924472788023 1101 60-61 2.3804540283212354 1101 62-63 2.4165307957821227 1101 64-65 2.2888872903598774 1101 66-67 2.4817162250157203 1101 68-69 2.820560035900872 1101 70-71 2.986898104558584 1101 72-73 3.2085483164195416 1101 74-75 3.5027373200257124 1101 76-77 3.7111959126311085 1101 78-79 3.8063857293514616 1101 80-81 4.048475662813043 1101 82-83 4.187809617775784 1101 84-85 4.352262364958495 1101 86-87 4.675434116803292 1101 88-89 5.2959180619359465 1101 90-91 5.36350825307365 1101 92-93 5.540707574647698 1101 94-95 5.743929709717317 1101 96-97 5.934622032900155 1101 98-99 6.02581385903401 1101 100-101 5.93994940360855 1102 1 0.675117789904224 1102 2 0.6849062158793124 1102 3 0.7136286971679091 1102 4 0.6201029854149311 1102 5 0.5271464229468634 1102 6 0.6094654069160015 1102 7 0.6521713840024432 1102 8 0.5779097673416302 1102 9 0.6734068870909198 1102 10-11 0.6950165060899138 1102 12-13 0.7675914423295467 1102 14-15 1.0705942531278367 1102 16-17 1.150919418036274 1102 18-19 1.114389627725778 1102 20-21 1.0054328589356558 1102 22-23 0.6993729193406253 1102 24-25 0.826617373388352 1102 26-27 0.8336908485323562 1102 28-29 0.777337562477534 1102 30-31 1.0157962813237162 1102 32-33 1.0015252089514135 1102 34-35 1.064514262855056 1102 36-37 1.2539915156347803 1102 38-39 1.2445340177356101 1102 40-41 1.5621668145416763 1102 42-43 1.630398960140802 1102 44-45 1.7021018319848054 1102 46-47 1.9563152097301852 1102 48-49 1.8106750285825015 1102 50-51 1.8980940893390326 1102 52-53 2.018887641700907 1102 54-55 2.188389332122682 1102 56-57 2.252380420037788 1102 58-59 2.2218645990663504 1102 60-61 2.1636063136256034 1102 62-63 2.4906311676381847 1102 64-65 2.3639343823674146 1102 66-67 2.50877094934447 1102 68-69 2.6094170421179896 1102 70-71 2.8398636810330764 1102 72-73 2.806636332113623 1102 74-75 3.0897428539465963 1102 76-77 3.2727473624793397 1102 78-79 3.7742753242137965 1102 80-81 4.1748091230263995 1102 82-83 4.126675407994309 1102 84-85 4.315296359043934 1102 86-87 4.562022898657496 1102 88-89 5.01307321668077 1102 90-91 5.226490031150735 1102 92-93 4.8602402657729264 1102 94-95 5.253074831180534 1102 96-97 5.305040932167177 1102 98-99 5.616240273502235 1102 100-101 5.28562669891692 1103 1 0.6054918004981715 1103 2 0.5191149931446226 1103 3 0.5087976430770205 1103 4 0.5616979851799542 1103 5 0.40378603409937597 1103 6 0.5181527109649338 1103 7 0.4240282784590832 1103 8 0.452019050135533 1103 9 0.6251802652706147 1103 10-11 0.6218853841924101 1103 12-13 0.6235053883393675 1103 14-15 0.7214351223953201 1103 16-17 0.8590981724084585 1103 18-19 0.9922124980271505 1103 20-21 0.9714496561804182 1103 22-23 0.8129339083774383 1103 24-25 0.8929544058751091 1103 26-27 1.099452867424219 1103 28-29 1.044503044206671 1103 30-31 0.9710700925259133 1103 32-33 1.1411366971389612 1103 34-35 1.2082408074697994 1103 36-37 1.2580938163605069 1103 38-39 1.3022921696358694 1103 40-41 1.4403253611973241 1103 42-43 1.604551880860754 1103 44-45 1.4773047483409698 1103 46-47 1.5379899661969958 1103 48-49 1.551046823712504 1103 50-51 1.6156311647923616 1103 52-53 1.8019195376680912 1103 54-55 1.9193494336312114 1103 56-57 2.095602778768402 1103 58-59 2.1622021552082202 1103 60-61 2.3771266858235442 1103 62-63 2.49558172251119 1103 64-65 2.539328969921879 1103 66-67 2.532228684334701 1103 68-69 2.810220986986508 1103 70-71 3.0593963524126373 1103 72-73 3.218520282084416 1103 74-75 3.537756992634641 1103 76-77 3.479728759295785 1103 78-79 3.5940939918472132 1103 80-81 4.017952933490104 1103 82-83 4.287051164105296 1103 84-85 4.4910944295762505 1103 86-87 4.625074666664855 1103 88-89 4.863591836648144 1103 90-91 5.230224766268524 1103 92-93 5.337972839134991 1103 94-95 5.272499750450368 1103 96-97 5.364629527227365 1103 98-99 5.502834031264751 1103 100-101 5.476617481244231 1104 1 0.7102768601242708 1104 2 0.6247840292327353 1104 3 0.6948141509143362 1104 4 0.6443009638812001 1104 5 0.637061433776509 1104 6 0.6114057183075374 1104 7 0.5836797652059502 1104 8 0.5927809102615527 1104 9 0.8322377562876468 1104 10-11 0.7813122966540007 1104 12-13 0.8617975822673998 1104 14-15 1.0142565565462291 1104 16-17 1.1048227987689216 1104 18-19 1.230747492559253 1104 20-21 1.1838738216753981 1104 22-23 1.0840764381904862 1104 24-25 1.0900749729740014 1104 26-27 0.9908197762405564 1104 28-29 1.0023234368536507 1104 30-31 1.1948277736174049 1104 32-33 1.2510680880674627 1104 34-35 1.3700416979556636 1104 36-37 1.5332874709978128 1104 38-39 1.5874933751766704 1104 40-41 1.7105741504490055 1104 42-43 1.826722570336365 1104 44-45 1.7200815931048083 1104 46-47 1.9290105749560453 1104 48-49 1.853294193915083 1104 50-51 1.8453289984641827 1104 52-53 2.068285144604509 1104 54-55 2.3010225446034553 1104 56-57 2.358506036071944 1104 58-59 2.4575300336647103 1104 60-61 2.7081638284575114 1104 62-63 2.776560280548999 1104 64-65 2.9256152923715 1104 66-67 3.093330465306426 1104 68-69 3.3502746244389883 1104 70-71 3.4351439569535955 1104 72-73 3.522373846631627 1104 74-75 3.679726020911474 1104 76-77 3.7616410907023337 1104 78-79 4.056120758498256 1104 80-81 4.469466694360801 1104 82-83 4.739802036364843 1104 84-85 4.941707940486005 1104 86-87 5.14572899150993 1104 88-89 5.364808117958358 1104 90-91 5.813405779651848 1104 92-93 5.805607453518164 1104 94-95 5.892902812471931 1104 96-97 6.004137676995629 1104 98-99 6.413086335592197 1104 100-101 5.978430674495284 1105 1 0.5213215011107728 1105 2 0.5353505342759846 1105 3 0.5675843463589523 1105 4 0.39035391051554313 1105 5 0.38243907886345596 1105 6 0.3485600237757964 1105 7 0.35083167625320755 1105 8 0.37923596997242726 1105 9 0.5557232850057332 1105 10-11 0.6471611188981683 1105 12-13 0.5837192547704717 1105 14-15 0.8809621325541315 1105 16-17 0.8659344087177416 1105 18-19 0.9075249760227777 1105 20-21 0.8950757836041063 1105 22-23 0.8871278284511774 1105 24-25 1.0973882455489061 1105 26-27 1.0135452633555673 1105 28-29 1.0655773053400779 1105 30-31 1.0759008109404462 1105 32-33 1.0584425306163254 1105 34-35 1.3304570551733477 1105 36-37 1.3125564617493595 1105 38-39 1.4083916197667818 1105 40-41 1.4057437802116581 1105 42-43 1.7142792507709501 1105 44-45 1.5883482193656207 1105 46-47 1.6374458582459752 1105 48-49 1.7081576745053084 1105 50-51 1.7597003881262694 1105 52-53 1.6822455543498052 1105 54-55 1.8674536975171705 1105 56-57 2.075267544257869 1105 58-59 2.1394901291422457 1105 60-61 2.407292670754952 1105 62-63 2.361299400898588 1105 64-65 2.5556800539769533 1105 66-67 2.7707364842822138 1105 68-69 2.923012644851429 1105 70-71 3.044216380706253 1105 72-73 3.2574236805186665 1105 74-75 3.424973656420736 1105 76-77 3.5472861626846175 1105 78-79 3.7110173494413594 1105 80-81 4.2344477260569455 1105 82-83 4.330427129896915 1105 84-85 4.488121359719191 1105 86-87 4.958803278421428 1105 88-89 5.016490011646063 1105 90-91 5.339912768368141 1105 92-93 5.512240373813874 1105 94-95 5.5943559267157426 1105 96-97 5.662696983810751 1105 98-99 5.77237294778579 1105 100-101 5.469789246522458 1106 1 0.19030460817349137 1106 2 0.21452585555431725 1106 3 0.14130588805024402 1106 4 0.2471326717742599 1106 5 0.22811152597690665 1106 6 0.1375650692368211 1106 7 0.2803910121472555 1106 8 0.21837569076431151 1106 9 0.2122610450532676 1106 10-11 0.022003699155220602 1106 12-13 -0.06847869233402548 1106 14-15 0.5386494659084633 1106 16-17 0.2718826360741602 1106 18-19 0.44961572638147373 1106 20-21 0.4564118888837143 1106 22-23 0.39465988834448495 1106 24-25 0.36950483158330627 1106 26-27 0.534056770206071 1106 28-29 0.30961141362015354 1106 30-31 0.5189531573670223 1106 32-33 0.6677256477464937 1106 34-35 0.6592529323356118 1106 36-37 0.4914695969330012 1106 38-39 0.5319666584152927 1106 40-41 0.6796873308351934 1106 42-43 0.32317665478890945 1106 44-45 0.7214949033754792 1106 46-47 1.033776889653339 1106 48-49 1.2533596072090205 1106 50-51 0.3916857637626663 1106 52-53 0.9878856744427722 1106 54-55 0.9596406754225058 1106 56-57 1.109324251805802 1106 58-59 0.34042720509715707 1106 60-61 1.5863410680227865 1106 62-63 2.124072094510197 1106 64-65 2.2191503046565053 1106 66-67 2.17652844996147 1106 68-69 1.6808231037604742 1106 70-71 1.978576594136232 1106 72-73 2.47083080889227 1106 74-75 2.693763814282086 1106 76-77 2.8433361083636086 1106 78-79 3.1887222668666375 1106 80-81 3.663609613772124 1106 82-83 3.944261445578853 1106 84-85 3.925431398327561 1106 86-87 4.025223446924631 1106 88-89 4.042925565439301 1106 90-91 4.573934085425552 1106 92-93 4.663286277071222 1106 94-95 4.573206094002206 1106 96-97 4.975862678628609 1106 98-99 5.064811889925117 1106 100-101 4.265680923210253 1107 1 0.2817071878318913 1107 2 0.1611569195903435 1107 3 0.3332145600571046 1107 4 0.3295413352569412 1107 5 0.39958897223199585 1107 6 0.25883726557532327 1107 7 0.21415057444313135 1107 8 0.22194610165382045 1107 9 0.18053528962884968 1107 10-11 0.3260991686788657 1107 12-13 0.3879123979564483 1107 14-15 0.566569786807058 1107 16-17 0.5994331296237974 1107 18-19 0.634360439567395 1107 20-21 0.6363687565666183 1107 22-23 0.6985306850137292 1107 24-25 0.5988565438461819 1107 26-27 0.6461575649025733 1107 28-29 0.5930847767962106 1107 30-31 0.737316905762647 1107 32-33 0.7198021059281174 1107 34-35 0.8563511515140263 1107 36-37 1.0387668860023282 1107 38-39 1.1422125321065337 1107 40-41 1.120066065945288 1107 42-43 1.2765030003296758 1107 44-45 1.3034333673681502 1107 46-47 1.2732302039737036 1107 48-49 1.3956062931321966 1107 50-51 1.3782595818860344 1107 52-53 1.437144314910185 1107 54-55 1.6897339907657738 1107 56-57 1.8298377662737622 1107 58-59 1.965876398588584 1107 60-61 1.8253780678060245 1107 62-63 2.111679925302923 1107 64-65 2.3205794432035525 1107 66-67 2.489803084213545 1107 68-69 2.48305177932313 1107 70-71 2.54169687996394 1107 72-73 2.6639313429926155 1107 74-75 2.7096999085545725 1107 76-77 2.953325952419952 1107 78-79 3.2445600165335833 1107 80-81 3.5546035506494036 1107 82-83 3.7765462041133766 1107 84-85 4.127753565805644 1107 86-87 4.005373843785787 1107 88-89 4.2974409418302635 1107 90-91 4.669770605176062 1107 92-93 5.149712748769748 1107 94-95 4.928604452977066 1107 96-97 4.930430509129071 1107 98-99 5.045197158205205 1107 100-101 4.930178137023313 1108 1 0.2436338949126018 1108 2 0.3422325983034433 1108 3 0.24743015295781845 1108 4 0.27682648285710343 1108 5 0.21373932486280012 1108 6 0.27762598710413755 1108 7 0.19490529007651247 1108 8 0.23435842015967268 1108 9 0.37021780285547834 1108 10-11 0.3385629769962861 1108 12-13 0.4566512963959184 1108 14-15 0.26136080365047576 1108 16-17 0.5806301219044983 1108 18-19 0.5257145561331882 1108 20-21 0.6059927440328678 1108 22-23 0.6431827091288156 1108 24-25 0.6053430695875193 1108 26-27 0.4306134753376867 1108 28-29 0.6534269667739849 1108 30-31 0.6631418142119756 1108 32-33 0.6061545676768461 1108 34-35 0.8889727746157448 1108 36-37 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2311 22-23 0.0860260940178037 2311 24-25 0.15718633952897676 2311 26-27 0.18337980964916056 2311 28-29 0.20636506723334946 2311 30-31 0.30573228476426806 2311 32-33 0.22715589761376265 2311 34-35 0.34233475152260695 2311 36-37 0.36356762485456073 2311 38-39 0.5403392268995049 2311 40-41 0.3212367997176102 2311 42-43 0.5636332760994733 2311 44-45 0.6816221717774198 2311 46-47 0.7625965332518589 2311 48-49 0.8542242781500491 2311 50-51 1.0071169128121724 2311 52-53 1.10673749304798 2311 54-55 0.9919219878752372 2311 56-57 1.1594967625985788 2311 58-59 1.1052925729653467 2311 60-61 1.1307374295025703 2311 62-63 1.3439576376275397 2311 64-65 1.4685669175357141 2311 66-67 1.644654693685542 2311 68-69 1.537605182741693 2311 70-71 1.6428931623304628 2311 72-73 1.8767794537679805 2311 74-75 1.8402566532641984 2311 76-77 2.210587477012183 2311 78-79 2.3065463615999917 2311 80-81 2.244144755135938 2311 82-83 2.376235506973625 2311 84-85 2.795207757206054 2311 86-87 3.250729335928625 2311 88-89 3.193527624603174 2311 90-91 3.448449274812763 2311 92-93 3.6018453558114985 2311 94-95 3.6570178286350092 2311 96-97 3.6765333796343675 2311 98-99 3.9695297943431385 2311 100-101 4.080727178399515 2312 1 -0.3100541051693817 2312 2 -0.32063807197294736 2312 3 -0.20228630775328682 2312 4 -0.27034315785247287 2312 5 -0.325809085996994 2312 6 -0.19487911129819224 2312 7 -0.3179653500585715 2312 8 -0.2619058660670177 2312 9 -0.4198482849774976 2312 10-11 -0.44995068274330663 2312 12-13 -0.38200032011259566 2312 14-15 -0.2737217202380151 2312 16-17 -0.2762859218920326 2312 18-19 -0.07061450376543377 2312 20-21 -0.2433103587737051 2312 22-23 -0.41197558398094003 2312 24-25 -0.389566515529431 2312 26-27 -0.3057272371907942 2312 28-29 -0.3048848908770623 2312 30-31 -0.09260191353242675 2312 32-33 -0.010330437323595731 2312 34-35 -0.09100797111639025 2312 36-37 -0.04341469758869465 2312 38-39 -0.32693644643598674 2312 40-41 -0.043890201963108666 2312 42-43 0.03599294145374898 2312 44-45 -0.09033936890197225 2312 46-47 -0.29383213715905043 2312 48-49 0.10747981610443702 2312 50-51 -0.028557014414367643 2312 52-53 0.05935410535630581 2312 54-55 0.2945118361337933 2312 56-57 0.22310991233626254 2312 58-59 0.4745736631150521 2312 60-61 0.5426797809771919 2312 62-63 0.8894086699832116 2312 64-65 0.7228439576038959 2312 66-67 0.9623104527565651 2312 68-69 0.9534262835607983 2312 70-71 1.1075780514958993 2312 72-73 1.2792837416012226 2312 74-75 1.3634512573419322 2312 76-77 1.430415275334731 2312 78-79 1.6378330678225055 2312 80-81 1.9358355819050637 2312 82-83 2.19098269463505 2312 84-85 2.295524910441035 2312 86-87 2.8014798916638135 2312 88-89 2.6940127890473526 2312 90-91 2.569988855264775 2312 92-93 2.601432196171263 2312 94-95 2.7606511578322177 2312 96-97 3.2668761617490425 2312 98-99 3.2966732807463117 2312 100-101 3.290085146131096 2313 1 -0.5155201772016227 2313 2 -0.6420572432272955 2313 3 -0.5025628876114254 2313 4 -0.49776965835035725 2313 5 -0.32975409151571 2313 6 -0.30213816686509887 2313 7 -0.38882513425511434 2313 8 -0.35655716255043757 2313 9 -0.40390751773221467 2313 10-11 -0.2487506464222662 2313 12-13 -0.31880630130540766 2313 14-15 -0.25679362793347593 2313 16-17 -0.23060530413975044 2313 18-19 -0.21311555750524036 2313 20-21 -0.12409511424164776 2313 22-23 -0.36050215816391784 2313 24-25 -0.2099360883092558 2313 26-27 -0.2683164062793679 2313 28-29 -0.20631236320834034 2313 30-31 -0.2129845545264928 2313 32-33 -0.1090304260959769 2313 34-35 -0.12334965399562492 2313 36-37 -0.10423833866313004 2313 38-39 0.0853864194418037 2313 40-41 0.19071446001495218 2313 42-43 0.22881490267980809 2313 44-45 0.23429409222990927 2313 46-47 0.1408175886845342 2313 48-49 0.2135230062457225 2313 50-51 0.41024248298088395 2313 52-53 0.4764080635834276 2313 54-55 0.4842327039859242 2313 56-57 0.6906416886501603 2313 58-59 0.7065148328368025 2313 60-61 0.855006113306171 2313 62-63 0.8949844413913084 2313 64-65 0.9257137190445626 2313 66-67 0.8555072508600183 2313 68-69 1.080786768103188 2313 70-71 1.1974747880621734 2313 72-73 1.337209214741133 2313 74-75 1.4114102231492254 2313 76-77 1.7656875205950477 2313 78-79 1.8449539868431373 2313 80-81 1.976911187966504 2313 82-83 2.0731241209355424 2313 84-85 2.2158549718125577 2313 86-87 2.316348973311932 2313 88-89 2.413723674747107 2313 90-91 2.5720010841683525 2313 92-93 2.9575063696373896 2313 94-95 3.2309056321617646 2313 96-97 3.462578498505266 2313 98-99 3.173716416283323 2313 100-101 3.2704438655442676 2314 1 -0.5761951565851753 2314 2 -0.6661887172567695 2314 3 -0.6372320013832109 2314 4 -0.5535537343924943 2314 5 -0.39213543068571965 2314 6 -0.3750335841233863 2314 7 -0.27455731265403216 2314 8 -0.26187961618668254 2314 9 -0.3984771765222561 2314 10-11 -0.40771709586701377 2314 12-13 -0.506171364332836 2314 14-15 -0.4854867621914991 2314 16-17 -0.49515958585033815 2314 18-19 -0.3165403679269261 2314 20-21 -0.342177753642936 2314 22-23 -0.3686916930692661 2314 24-25 -0.49825074799836244 2314 26-27 -0.3426887672191157 2314 28-29 -0.2535739409711013 2314 30-31 -0.3189515434091206 2314 32-33 -0.03968320792126434 2314 34-35 -0.2670506318042598 2314 36-37 -0.37529656695075886 2314 38-39 -0.25625725394406373 2314 40-41 -0.14952512480389402 2314 42-43 -0.08417159897260973 2314 44-45 -0.017530075826691416 2314 46-47 -0.026630436166804827 2314 48-49 -0.0752650566762938 2314 50-51 -0.0639826176211713 2314 52-53 -0.035171559306100164 2314 54-55 -0.13883508537288947 2314 56-57 -0.11433199050211584 2314 58-59 0.01748681306981581 2314 60-61 0.14842032098355062 2314 62-63 0.2919149159106169 2314 64-65 0.49210611979109586 2314 66-67 0.8430983197530537 2314 68-69 1.0689544774763817 2314 70-71 1.1040971629528933 2314 72-73 1.0052416306167267 2314 74-75 0.9971718536156828 2314 76-77 1.2021671614658374 2314 78-79 1.676333149660369 2314 80-81 1.9736525492100618 2314 82-83 1.968617024537373 2314 84-85 2.3484587205957226 2314 86-87 2.5253895179581676 2314 88-89 2.2428923823903837 2314 90-91 2.7568918635010604 2314 92-93 2.759155065375829 2314 94-95 2.7070655785900044 2314 96-97 2.8029461223124557 2314 98-99 2.8862744678585415 2314 100-101 2.8279560028963093 2315 1 -0.4708689349921116 2315 2 -0.4021742072843253 2315 3 -0.3226025870906355 2315 4 -0.43758012535283086 2315 5 -0.3067774843271138 2315 6 -0.2867919703313291 2315 7 -0.3562865019662027 2315 8 -0.2726546610563716 2315 9 -0.3042426154868423 2315 10-11 -0.360256025061382 2315 12-13 -0.22614216743706095 2315 14-15 -0.2585971795428037 2315 16-17 -0.12114893087927925 2315 18-19 -0.1879191944897869 2315 20-21 -0.18647772330849932 2315 22-23 -0.22043876706000987 2315 24-25 -0.28755817487972735 2315 26-27 -0.3177273172330146 2315 28-29 -0.3194505430085641 2315 30-31 -0.23401353941508773 2315 32-33 -0.060884135649466486 2315 34-35 -0.3509439936182659 2315 36-37 -0.055152920751403656 2315 38-39 0.017822601461780607 2315 40-41 0.012877868193903907 2315 42-43 -0.04924686629484398 2315 44-45 -0.12859329784949836 2315 46-47 0.08680708753197308 2315 48-49 0.03513681141946279 2315 50-51 0.3536653891535124 2315 52-53 0.4193880857809802 2315 54-55 0.3775723693150397 2315 56-57 0.6800978484725775 2315 58-59 0.9112072685919443 2315 60-61 0.9182691543716537 2315 62-63 0.9489149032712696 2315 64-65 0.9641112260880185 2315 66-67 1.1479654170002043 2315 68-69 1.342659260205977 2315 70-71 1.5063943646010607 2315 72-73 1.4594025042485193 2315 74-75 1.361940616729008 2315 76-77 1.4481984059361217 2315 78-79 1.8719965890799664 2315 80-81 2.1189115294674004 2315 82-83 2.4787471466334843 2315 84-85 2.7736539985350035 2315 86-87 2.977793522103873 2315 88-89 3.0143650561182334 2315 90-91 2.9577690343092193 2315 92-93 3.3152712462701928 2315 94-95 3.287989641382289 2315 96-97 3.570882306240261 2315 98-99 3.5644254575195546 2315 100-101 3.039872064980793 2316 1 -1.0376971094204954 2316 2 -0.6717967329453707 2316 3 -0.7175174284526413 2316 4 -0.6217777254275774 2316 5 -0.6723252759420575 2316 6 -0.4154449155121256 2316 7 -0.6016217974186233 2316 8 -0.7384357045833809 2316 9 -0.7511015564764421 2316 10-11 -0.6622877192911432 2316 12-13 -0.501821567856183 2316 14-15 -0.9857314845922502 2316 16-17 -0.7150488427841353 2316 18-19 -0.6108763215203155 2316 20-21 -0.4933609905704728 2316 22-23 -0.6557106940745214 2316 24-25 -0.9263031861585844 2316 26-27 -0.7310764009100552 2316 28-29 -0.6909736212664157 2316 30-31 -0.45100605800182336 2316 32-33 -0.46711436429639974 2316 34-35 -0.5846352134690349 2316 36-37 -0.3380526190922808 2316 38-39 -0.3540193211479661 2316 40-41 -0.30614334137910504 2316 42-43 -0.19443016425663728 2316 44-45 -0.35495777148101837 2316 46-47 0.2331568119009546 2316 48-49 -0.07079743089557411 2316 50-51 0.09594388326048175 2316 52-53 0.2639525487343022 2316 54-55 0.12274432169027705 2316 56-57 0.46344853387948604 2316 58-59 0.7025307312649645 2316 60-61 0.5330357689821028 2316 62-63 0.6639910188093836 2316 64-65 0.9094962419050994 2316 66-67 1.0388070259378566 2316 68-69 1.1907553706719511 2316 70-71 1.1521888226157166 2316 72-73 1.3747337286375227 2316 74-75 1.7706248290116093 2316 76-77 1.7629316511743127 2316 78-79 1.6735209020045936 2316 80-81 2.3037731800500296 2316 82-83 2.309444466405502 2316 84-85 2.1713415010377055 2316 86-87 2.6751019485377547 2316 88-89 2.7007778819701542 2316 90-91 2.8371500991926304 2316 92-93 3.108478576853887 2316 94-95 3.1343920893594373 2316 96-97 3.2727117820741576 2316 98-99 3.1626418645725067 2316 100-101 2.789927624985596 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4793.0 3 1749.0 4 1966.0 5 2229.0 6 2710.0 7 3453.0 8 4407.0 9 5724.0 10 7137.0 11 8964.0 12 11336.0 13 13310.0 14 15348.0 15 17996.0 16 21044.0 17 24151.0 18 23869.0 19 23081.0 20 24846.0 21 27349.0 22 30599.0 23 34154.0 24 37801.0 25 42521.0 26 47823.0 27 53245.0 28 59053.0 29 66519.0 30 74728.0 31 84925.0 32 96675.0 33 111626.0 34 131171.0 35 154037.0 36 175233.0 37 169066.0 38 84221.0 39 5808.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.81722960491172 18.01653398220467 14.489355042911637 23.676881369971973 2 15.718886512852896 19.610727841638635 39.69374174027553 24.97664390523294 3 19.5383761234534 23.39400597923115 29.231033782538624 27.836584114776823 4 13.236764819809984 15.913732753945729 35.59107865163264 35.25842377461165 5 14.48044915724319 36.80541900113581 33.408036345813514 15.306095495807496 6 33.52104805626042 34.87402628123516 16.819368308847658 14.78555735365677 7 29.71928296802603 32.089985775783155 19.810155996939212 18.380575259251607 8 27.41061793292362 34.366302313749586 19.120694177688506 19.10238557563829 9 26.83642659076571 16.57641690752505 19.236843263545534 37.35031323816371 10-11 25.68517457358227 26.164251769032447 26.615073737925876 21.5354999194594 12-13 26.575019629429235 23.316827790342437 27.014525190807444 23.09362738942088 14-15 23.199933340101033 24.595096859297737 25.21519235429736 26.98977744630387 16-17 22.959922664722527 27.92299330944233 25.9079047156623 23.209179310172846 18-19 22.99148250629255 26.59141526060491 28.154818819521317 22.262283413581223 20-21 23.450693314741653 25.58844311110172 28.430423218942856 22.53044035521377 22-23 23.149068042328476 25.459611842255832 28.493469577214025 22.897850538201666 24-25 23.098276746034937 25.603464644665262 28.64192538171518 22.656333227584625 26-27 22.633886388046037 25.970144435901798 28.362961963060563 23.033007212991603 28-29 22.708284123502615 26.38695432105105 28.32537906852209 22.579382486924246 30-31 22.75476687486319 25.908172481321877 28.171290006625593 23.165770637189343 32-33 22.47252265217913 25.85665449298172 28.30811599535352 23.36270685948563 34-35 23.212411547391408 25.95897929129938 28.10713398144054 22.721475179868676 36-37 23.28896986981436 25.593640063259016 28.149556512069385 22.967833554857243 38-39 22.721131902437136 25.96521831479071 28.113762907300377 23.19988687547178 40-41 23.09311044874202 25.76708012579797 28.26605332331956 22.873756102140444 42-43 22.910871313979666 25.797991094348387 28.026310925646786 23.264826666025158 44-45 22.73462484137754 26.083311479660832 28.05738745065323 23.124676228308395 46-47 23.30126423344388 26.252169027967444 27.546354492876496 22.900212245712183 48-49 22.966604446580053 26.451105308596667 27.41110169449263 23.17118855033065 50-51 23.139662175956673 26.424711185786798 27.37789195544177 23.05773468281476 52-53 23.388836872043253 26.194109268228583 27.285143050236538 23.131910809491625 54-55 23.063808817015683 26.566972417859315 27.23263392870238 23.13658483642262 56-57 22.977710612245378 26.656685701681006 27.15202824394073 23.213575442132893 58-59 23.08289359784832 26.146468070202406 27.357917453418935 23.412720878530337 60-61 22.774657017896377 26.387108507598846 27.58332917775229 23.254905296752487 62-63 22.840415839737087 26.413308936588603 27.736318043129206 23.0099571805451 64-65 22.812143228424155 26.5176102156499 27.345965613820177 23.324280942105762 66-67 22.619329416330157 26.6255607189757 27.258751879818337 23.496357984875804 68-69 23.067168506913795 26.21549151626566 27.469740130176913 23.247599846643627 70-71 23.089706698145893 26.17885014971505 27.072857411488883 23.65858574065018 72-73 22.849658883691895 26.410815745562672 27.09844614305059 23.641079227694842 74-75 22.679274053426603 26.69960201249611 27.121966874368013 23.499157059709272 76-77 23.040011311364374 26.419692099733616 26.997404781104862 23.542891807797155 78-79 22.97808956596831 26.605267233611173 27.01117462641736 23.405468574003155 80-81 23.040355939989098 26.426631670281488 26.940020192929893 23.59299219679952 82-83 23.07565492752199 26.370232607257627 27.010338915183933 23.54377355003645 84-85 22.901336796908595 26.54662656334606 26.91410345084498 23.637933188900366 86-87 23.21681140001725 26.39979139155793 26.732473752353155 23.650923456071673 88-89 23.101778860794475 26.445130033392143 26.607807460336925 23.845283645476453 90-91 22.882270702127315 26.405919692748697 26.770443199439015 23.94136640568497 92-93 23.13492886522603 26.07381283385908 26.693999755339913 24.09725854557498 94-95 23.12660206149393 25.818109378937766 26.954944097093765 24.10034446247454 96-97 23.152475384625316 25.844339182583564 26.856091076739652 24.147094356051472 98-99 22.95883598668985 25.91381140785379 26.585420732703653 24.54193187275271 100-101 23.423249892238722 25.27592172596571 26.856694529955288 24.44413385184028 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 421.0 1 308.5 2 258.5 3 342.0 4 302.0 5 288.0 6 552.0 7 1218.5 8 1997.0 9 2281.5 10 1922.0 11 1559.5 12 1556.0 13 1666.0 14 1752.0 15 1884.0 16 2006.0 17 2053.0 18 2169.0 19 2337.5 20 2614.0 21 3058.5 22 3665.0 23 4328.0 24 5201.5 25 6477.0 26 8150.5 27 10172.5 28 11930.5 29 13828.0 30 16236.0 31 19531.5 32 23792.5 33 26341.0 34 27512.0 35 29512.5 36 32330.5 37 35350.5 38 37984.0 39 39991.0 40 42208.0 41 45267.5 42 47549.5 43 50155.5 44 53657.5 45 56779.5 46 58489.5 47 60919.5 48 63934.5 49 65455.0 50 65475.0 51 64381.0 52 63706.0 53 63975.0 54 64724.5 55 62255.0 56 58001.5 57 54391.0 58 50686.5 59 45388.0 60 37706.0 61 31411.5 62 26570.0 63 20511.0 64 15028.5 65 11736.5 66 9221.5 67 7200.5 68 5764.0 69 4728.5 70 3807.0 71 2609.0 72 1827.0 73 1357.5 74 953.5 75 687.0 76 481.5 77 330.0 78 232.0 79 153.5 80 88.5 81 56.5 82 42.5 83 28.0 84 13.5 85 10.0 86 13.0 87 8.5 88 3.5 89 3.5 90 3.0 91 2.5 92 2.0 93 3.0 94 2.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2871528574202469 2 0.036077427438907426 3 0.045111449919544404 4 0.0468713244287594 5 0.060539683116995875 6 0.05971840834602887 7 0.031149778813105433 8 0.03226436600227493 9 0.040594438679225915 10-11 0.03270433462957868 12-13 0.03478685279881642 14-15 0.028979266918406937 16-17 0.0234356622143797 18-19 0.02748337358557419 20-21 0.03983182639189941 22-23 0.03320296574052293 24-25 0.055289390831171126 26-27 0.046753999461478395 28-29 0.03839459554270717 30-31 0.03918653907185392 32-33 0.03411223423695068 34-35 0.033818921818748175 36-37 0.03296831580596093 38-39 0.020561200515995207 40-41 0.020355881823253458 42-43 0.020883844176017956 44-45 0.008682047578793982 46-47 0.016102851759317215 48-49 0.01369768993005672 50-51 0.006511535684095486 52-53 0.005338286011285488 54-55 0.007156823004140985 56-57 0.005220961044004489 58-59 0.008124753984209232 60-61 0.007743447840545984 62-63 0.0039890488875539915 64-65 0.008506060127872483 66-67 0.002786467972923744 68-69 0.008242078951490232 70-71 0.015398901955631218 72-73 0.015428233197451466 74-75 0.012700427708168222 76-77 0.010940553198953226 78-79 0.005338286011285488 80-81 0.0064528732004549855 82-83 0.010823228231672226 84-85 0.004106373854834992 86-87 0.002229174378338995 88-89 0.021939768881546952 90-91 0.030768472669442184 92-93 0.015340239471990718 94-95 0.024491586919908697 96-97 0.020297219339612958 98-99 0.014870939602866718 100-101 0.10729368257847427 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1704667.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.48552489803156 #Duplication Level Percentage of deduplicated Percentage of total 1 78.19298001036564 43.385785392164266 2 12.15854601460625 13.49246615234595 3 3.9532766993768114 6.580488981962403 4 1.785252100195969 3.9622259941874627 5 0.9990347331797113 2.7715983280920593 6 0.6126661266648314 2.0396460975145234 7 0.4184417097533157 1.6252220533424746 8 0.3071004697180975 1.363170460699255 9 0.22084447555164988 1.102830448213241 >10 1.2106733178034668 12.195667107855098 >50 0.08453756447421103 3.222441674066687 >100 0.051633178291827155 5.282262589399105 >500 0.0036268595876020196 1.393397026926016 >1k 0.0012800680897418894 1.22193023621135 >5k 1.066723408118241E-4 0.36086745702013084 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6097 0.35766516275612775 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4649 0.2727218864446839 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3329 0.1952874080392241 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.866248364049988E-5 0.0 10-11 0.0 0.0 0.0 5.866248364049988E-5 0.0 12-13 0.0 0.0 0.0 5.866248364049988E-5 0.0 14-15 0.0 0.0 0.0 5.866248364049988E-5 0.0 16-17 0.0 0.0 0.0 1.1732496728099975E-4 0.0 18-19 0.0 0.0 0.0 1.466562091012497E-4 5.866248364049988E-5 20-21 0.0 0.0 0.0 1.7598745092149964E-4 5.866248364049988E-5 22-23 0.0 0.0 0.0 2.346499345619995E-4 5.866248364049988E-5 24-25 0.0 0.0 0.0 4.399686273037491E-4 5.866248364049988E-5 26-27 0.0 0.0 0.0 9.67930980068248E-4 5.866248364049988E-5 28-29 0.0 0.0 0.0 0.003197105358407243 5.866248364049988E-5 30-31 0.0 0.0 0.0 0.010500584571649478 5.866248364049988E-5 32-33 0.0 0.0 0.0 0.024990218030852947 5.866248364049988E-5 34-35 0.0 0.0 0.0 0.0464020245596354 5.866248364049988E-5 36-37 0.0 0.0 0.0 0.08731910689888406 5.866248364049988E-5 38-39 0.0 0.0 0.0 0.14829875864318368 5.866248364049988E-5 40-41 0.0 0.0 0.0 0.21608325848978127 5.866248364049988E-5 42-43 0.0 0.0 0.0 0.28844343206033785 5.866248364049988E-5 44-45 0.0 5.866248364049988E-5 0.0 0.37904763804308994 5.866248364049988E-5 46-47 0.0 5.866248364049988E-5 0.0 0.48625332689610345 5.866248364049988E-5 48-49 0.0 5.866248364049988E-5 0.0 0.6001465388841339 5.866248364049988E-5 50-51 0.0 5.866248364049988E-5 0.0 0.720081986687136 5.866248364049988E-5 52-53 0.0 5.866248364049988E-5 0.0 0.839430809653733 1.1732496728099975E-4 54-55 0.0 5.866248364049988E-5 0.0 0.9722720038576449 1.1732496728099975E-4 56-57 0.0 5.866248364049988E-5 0.0 1.137846863932956 1.1732496728099975E-4 58-59 0.0 5.866248364049988E-5 0.0 1.3102265721105648 1.1732496728099975E-4 60-61 0.0 5.866248364049988E-5 0.0 1.4968026013291746 1.1732496728099975E-4 62-63 0.0 5.866248364049988E-5 0.0 1.6809148062348833 1.1732496728099975E-4 64-65 0.0 5.866248364049988E-5 0.0 1.880983206690808 1.1732496728099975E-4 66-67 0.0 5.866248364049988E-5 0.0 2.113374635632648 1.1732496728099975E-4 68-69 0.0 5.866248364049988E-5 0.0 2.370902938814443 1.1732496728099975E-4 70-71 0.0 5.866248364049988E-5 0.0 2.640251732449798 1.466562091012497E-4 72-73 0.0 5.866248364049988E-5 0.0 2.9103338071306597 1.7598745092149964E-4 74-75 0.0 5.866248364049988E-5 0.0 3.190241847821305 2.0531869274174956E-4 76-77 0.0 5.866248364049988E-5 0.0 3.4993638053649185 2.346499345619995E-4 78-79 0.0 5.866248364049988E-5 0.0 3.850136126293288 2.346499345619995E-4 80-81 0.0 5.866248364049988E-5 0.0 4.214782124602635 2.346499345619995E-4 82-83 0.0 5.866248364049988E-5 0.0 4.594885687351254 2.346499345619995E-4 84-85 0.0 5.866248364049988E-5 0.0 4.977834380556438 2.346499345619995E-4 86-87 0.0 5.866248364049988E-5 0.0 5.377443219115522 2.346499345619995E-4 88-89 0.0 5.866248364049988E-5 0.0 5.805386037272969 2.346499345619995E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3650 0.0 45.445854 1 GTATCAA 6175 0.0 39.136223 1 ATCAACG 7965 0.0 30.316048 3 TCAACGC 8000 0.0 30.064579 4 TATCAAC 8050 0.0 30.054987 2 CAACGCA 8185 0.0 29.32698 5 AACGCAG 8445 0.0 28.53162 6 ACGCAGA 9605 0.0 24.778057 7 CGCAGAG 9630 0.0 24.713732 8 TACATGG 6870 0.0 24.147007 2 GAGTACT 5170 0.0 23.291035 12-13 GTACATG 7360 0.0 23.120558 1 ACATGGG 6935 0.0 22.755493 3 GTGGTAT 2160 0.0 21.846876 1 GTACTTT 5485 0.0 21.696827 14-15 AGAGTAC 8520 0.0 21.40852 10-11 GCAGAGT 10860 0.0 21.213547 9 AGTACTT 5475 0.0 21.169329 12-13 TATAGCG 115 0.0037090802 20.666492 5 TGGTATC 2105 0.0 20.54868 2 >>END_MODULE