FastQCFastQC Report
Thu 26 May 2016
SRR938497_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938497_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1704667
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56230.3298591455105308No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48120.2822838712780854No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35400.20766519208736955No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40400.040.151331
GTATCAA64500.037.022941
TATCAAC82500.028.5992532
ATCAACG82350.028.5793063
TCAACGC83650.028.0783184
CAACGCA85950.027.326955
TATACGA700.00835352327.1688965
TAAGGTG7850.026.6497465
AACGCAG88300.026.6253786
TACATGG63050.024.2184772
ACGCAGA99250.023.6400137
CGCAGAG100000.023.367728
GTACATG66050.023.2625121
GAGTACT54050.022.84698512-13
ACATGGG62800.022.4857233
AGGTAAG8000.022.0008092
GTACTTT56400.021.47396914-15
GTAAGGT8950.021.2494164
AGAGTAC85150.021.22370510-11
GGACCGA5750.020.6501586
Produced by FastQC (version 0.11.5)