##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938497_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1704667 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.711071429199954 31.0 31.0 34.0 30.0 34.0 2 31.95216309109052 31.0 31.0 34.0 30.0 34.0 3 32.014870939602865 33.0 31.0 34.0 30.0 34.0 4 35.70897131228563 37.0 35.0 37.0 33.0 37.0 5 35.572257220911766 37.0 35.0 37.0 33.0 37.0 6 35.61191540635209 37.0 35.0 37.0 33.0 37.0 7 35.54151279986062 37.0 35.0 37.0 33.0 37.0 8 35.51875762245647 37.0 35.0 37.0 33.0 37.0 9 37.069989035981806 39.0 37.0 39.0 33.0 39.0 10-11 36.99796118538107 39.0 37.0 39.0 33.0 39.0 12-13 36.877786101332404 39.0 37.0 39.0 32.5 39.0 14-15 37.884971668953526 40.0 37.0 41.0 32.5 41.0 16-17 37.797417618807664 40.0 37.0 41.0 32.0 41.0 18-19 37.78939875060642 39.5 37.0 41.0 32.5 41.0 20-21 37.7719824458384 39.5 37.0 41.0 32.0 41.0 22-23 37.801509620354004 40.0 37.0 41.0 32.0 41.0 24-25 37.71791528785388 40.0 37.0 41.0 32.0 41.0 26-27 37.52392285414102 39.0 36.5 41.0 32.0 41.0 28-29 37.38892698691299 39.0 36.0 41.0 31.5 41.0 30-31 37.124381184125696 39.0 36.0 40.5 31.0 41.0 32-33 36.89448965692419 39.0 35.5 40.0 30.5 41.0 34-35 36.715986172079354 39.0 35.0 40.0 30.0 41.0 36-37 36.732875101119454 39.0 35.0 40.0 30.0 41.0 38-39 36.57672642222792 39.0 35.0 40.0 30.0 41.0 40-41 36.38148917061221 39.0 35.0 40.0 30.0 41.0 42-43 36.13213636446297 38.0 35.0 40.0 30.0 41.0 44-45 35.82565421868318 38.0 35.0 40.0 29.0 41.0 46-47 35.44626369842321 38.0 34.0 40.0 27.5 41.0 48-49 35.01424090452856 38.0 34.0 40.0 26.0 41.0 50-51 34.60889604831911 38.0 33.0 40.0 25.0 41.0 52-53 34.059809041883256 37.0 33.0 40.0 24.0 41.0 54-55 33.69601188971218 37.0 32.5 40.0 23.0 41.0 56-57 33.29216468671007 36.5 32.0 39.0 22.0 41.0 58-59 32.78621367105716 36.0 31.0 39.0 20.5 40.0 60-61 32.33307942254997 35.5 31.0 39.0 19.0 40.0 62-63 31.80925189494488 35.0 30.0 38.5 17.5 40.0 64-65 31.265545411508526 35.0 29.5 38.0 13.5 40.0 66-67 30.730272246720325 34.0 29.0 37.5 10.0 40.0 68-69 30.12874567290855 34.0 28.0 37.0 7.5 39.0 70-71 29.529539200324756 34.0 28.0 36.0 6.5 39.0 72-73 28.910634745671736 33.0 26.5 36.0 2.0 39.0 74-75 28.2737405604731 33.0 26.0 35.0 2.0 38.0 76-77 27.048405348375958 31.5 25.5 34.0 2.0 36.0 78-79 27.666068211562727 32.5 26.0 35.0 2.0 36.5 80-81 27.35668256615515 32.0 26.0 35.0 2.0 36.0 82-83 26.83944459533739 32.0 25.0 35.0 2.0 36.0 84-85 26.202480601783222 32.0 23.5 34.5 2.0 35.5 86-87 25.52580357336653 31.5 21.0 34.0 2.0 35.0 88-89 24.722991645875705 31.0 18.5 34.0 2.0 35.0 90-91 24.133840216300307 31.0 14.5 34.0 2.0 35.0 92-93 23.30359096527357 30.0 4.5 34.0 2.0 35.0 94-95 22.453888354734385 30.0 2.0 34.0 2.0 35.0 96-97 21.418372913888753 29.0 2.0 34.0 2.0 35.0 98-99 20.203655024705704 29.0 2.0 34.0 2.0 35.0 100-101 17.203051094436624 22.5 2.0 31.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.35799461863295434 1101 2 0.3505744705583389 1101 3 0.18391382338697326 1101 4 0.3500356380616978 1101 5 0.26866617484325417 1101 6 0.2751842419464694 1101 7 0.25710689983837653 1101 8 0.2236403939175986 1101 9 0.3592687720642118 1101 10-11 0.3458486514056034 1101 12-13 0.41232161906667386 1101 14-15 0.6865520424700549 1101 16-17 0.6516839292636121 1101 18-19 0.6777949704173452 1101 20-21 0.7734718818375228 1101 22-23 0.6641469015741563 1101 24-25 0.7943394415691998 1101 26-27 0.7883986961294696 1101 28-29 0.6864493564382954 1101 30-31 0.6730171714068618 1101 32-33 0.8033458687490267 1101 34-35 0.7753649250278158 1101 36-37 1.0273112187387525 1101 38-39 1.1275590367117942 1101 40-41 1.1885477314266026 1101 42-43 1.0968376898089147 1101 44-45 1.0692980300288681 1101 46-47 1.2631958975889361 1101 48-49 1.152677010531555 1101 50-51 1.2937669657556583 1101 52-53 1.4319328728188125 1101 54-55 1.3707906571835053 1101 56-57 1.491515822782759 1101 58-59 1.7338999155300172 1101 60-61 1.7330828812804384 1101 62-63 1.7942874534748263 1101 64-65 1.7329024282007666 1101 66-67 1.7755210872355534 1101 68-69 1.8942176946497788 1101 70-71 2.0171085378050115 1101 72-73 2.2940617497635962 1101 74-75 2.280384924143142 1101 76-77 2.0602458148620286 1101 78-79 2.3864993081796584 1101 80-81 2.8269686135044623 1101 82-83 2.7862339422613367 1101 84-85 3.063421123912832 1101 86-87 3.200163161147838 1101 88-89 3.202890714275412 1101 90-91 3.5843722636167854 1101 92-93 3.8329169177800715 1101 94-95 4.169597004718842 1101 96-97 4.447045732393196 1101 98-99 4.513565064485043 1101 100-101 4.781641999937587 1102 1 0.342373736133613 1102 2 0.32902438711032644 1102 3 0.3256144060590671 1102 4 0.24564754135960243 1102 5 0.24453457098219644 1102 6 0.3009604060706508 1102 7 0.39005276110971465 1102 8 0.40081370226533153 1102 9 0.44264358540418414 1102 10-11 0.4192072631429795 1102 12-13 0.5340483585429538 1102 14-15 0.6255861927155237 1102 16-17 0.5575340741996371 1102 18-19 0.6856371316720882 1102 20-21 0.5824369312719284 1102 22-23 0.6285131358727511 1102 24-25 0.599305330363741 1102 26-27 0.7553147308360266 1102 28-29 0.6646186729453873 1102 30-31 0.7529515841963743 1102 32-33 0.6667985669014556 1102 34-35 0.6520595052989506 1102 36-37 0.8704460336403272 1102 38-39 1.0653853867640066 1102 40-41 0.9666662959255703 1102 42-43 0.9474145059012002 1102 44-45 0.8799735197369429 1102 46-47 0.9478868471394861 1102 48-49 1.158491531461884 1102 50-51 1.1446487818967412 1102 52-53 1.4215165111521983 1102 54-55 1.3429185327239637 1102 56-57 1.4644537784528282 1102 58-59 1.5313554831229084 1102 60-61 1.6160897425615417 1102 62-63 1.8842551478696556 1102 64-65 1.6319254520444488 1102 66-67 1.6618292690448655 1102 68-69 1.8903600540325591 1102 70-71 2.032836780321528 1102 72-73 2.239713181067831 1102 74-75 2.121499418721463 1102 76-77 1.9596470387662244 1102 78-79 2.476635362601428 1102 80-81 2.6075588983989064 1102 82-83 2.7381073745610855 1102 84-85 2.733733151162756 1102 86-87 2.8556643860280353 1102 88-89 2.9510631539830037 1102 90-91 3.1374276321056485 1102 92-93 3.579649757257325 1102 94-95 3.8401164320019525 1102 96-97 4.066854631562617 1102 98-99 4.337489756312994 1102 100-101 4.622356334451883 1103 1 0.30223959986244253 1103 2 0.2577690246805666 1103 3 0.3136468726760526 1103 4 0.162665661169207 1103 5 0.26218913315953074 1103 6 0.17477899314174294 1103 7 0.2892672784406898 1103 8 0.16598056698104813 1103 9 0.339167321079195 1103 10-11 0.3314463586966099 1103 12-13 0.45901093062945364 1103 14-15 0.5310305275056848 1103 16-17 0.5842183303072659 1103 18-19 0.506038869132901 1103 20-21 0.47626317176388966 1103 22-23 0.49565881269698053 1103 24-25 0.5490636433886564 1103 26-27 0.541390875660241 1103 28-29 0.42920414621547565 1103 30-31 0.5506535438749225 1103 32-33 0.4102347514697513 1103 34-35 0.5727509314535055 1103 36-37 0.6396171815863241 1103 38-39 0.830903033269955 1103 40-41 0.8188418143776985 1103 42-43 0.8071635542587359 1103 44-45 0.8781638885109899 1103 46-47 0.8544677820652709 1103 48-49 0.997070143507294 1103 50-51 1.0146379731181412 1103 52-53 1.1329654245678427 1103 54-55 1.1869784039585198 1103 56-57 1.1779892560867182 1103 58-59 1.4202952829255295 1103 60-61 1.6021830068798124 1103 62-63 1.5487379041753364 1103 64-65 1.546274944837478 1103 66-67 1.4671628306381166 1103 68-69 1.5902835198237817 1103 70-71 1.6583283783423788 1103 72-73 1.697760685985486 1103 74-75 1.742869918140741 1103 76-77 1.6480782888921048 1103 78-79 1.8797870278918225 1103 80-81 2.3647813108377314 1103 82-83 2.6458547848180167 1103 84-85 2.5990708751428073 1103 86-87 2.655298163125508 1103 88-89 2.951720072608339 1103 90-91 3.40636984877802 1103 92-93 3.6637585029690563 1103 94-95 3.6391325646075394 1103 96-97 4.009389860796002 1103 98-99 4.078914903842755 1103 100-101 4.252961626053931 1104 1 0.37395235307808505 1104 2 0.3164486580711845 1104 3 0.28099116783826616 1104 4 0.24338351775411127 1104 5 0.21548679498320666 1104 6 0.2563469773108835 1104 7 0.3299894312293148 1104 8 0.33549053938119044 1104 9 0.4048653890894016 1104 10-11 0.49721798292199537 1104 12-13 0.43051538305875425 1104 14-15 0.5382839514498627 1104 16-17 0.6554256286460642 1104 18-19 0.5885038485631995 1104 20-21 0.6125078340562453 1104 22-23 0.5631801635275835 1104 24-25 0.6599346661455172 1104 26-27 0.6502495175391161 1104 28-29 0.6098308712063627 1104 30-31 0.7636017160355308 1104 32-33 0.6201212275411976 1104 34-35 0.67922680761869 1104 36-37 0.7850693895746588 1104 38-39 0.7456822995304364 1104 40-41 0.8138113503877022 1104 42-43 0.8582926096146721 1104 44-45 1.0452286049615438 1104 46-47 1.003615376191192 1104 48-49 1.0363078277232987 1104 50-51 1.0973789510903273 1104 52-53 1.2760039471945177 1104 54-55 1.1861810025108284 1104 56-57 1.4272553212848607 1104 58-59 1.486434037286827 1104 60-61 1.3722716649020015 1104 62-63 1.4159259737478003 1104 64-65 1.4467149802486148 1104 66-67 1.7448290807032123 1104 68-69 1.9737532250781662 1104 70-71 2.1371741316100987 1104 72-73 2.2950199032952803 1104 74-75 2.2659555037263246 1104 76-77 2.2830737974063986 1104 78-79 2.468149197865266 1104 80-81 2.8129866693754586 1104 82-83 2.9334735813602215 1104 84-85 3.2864393852714286 1104 86-87 3.4966263410143803 1104 88-89 3.585238923829504 1104 90-91 3.6968013568757137 1104 92-93 3.990575927329836 1104 94-95 4.264460963815996 1104 96-97 4.5148164217500195 1104 98-99 4.73374097268616 1104 100-101 4.677124517268844 1105 1 0.3620101783452121 1105 2 0.3595594599029148 1105 3 0.3530838169657997 1105 4 0.23215592803815355 1105 5 0.11341337604932278 1105 6 0.1716218867718382 1105 7 0.21382447058761045 1105 8 0.1199477540709637 1105 9 0.341787360895097 1105 10-11 0.3663684872469162 1105 12-13 0.41953845929113953 1105 14-15 0.5528543773574981 1105 16-17 0.5211107697672546 1105 18-19 0.49964464051793556 1105 20-21 0.49736143765984764 1105 22-23 0.43081170703121785 1105 24-25 0.4252243536735776 1105 26-27 0.5183674278081867 1105 28-29 0.6301198478906898 1105 30-31 0.7093503093131872 1105 32-33 0.7270246425841478 1105 34-35 0.8329065463275 1105 36-37 0.8224083273496419 1105 38-39 0.9415637083478856 1105 40-41 0.9211337891054896 1105 42-43 1.0074042420111837 1105 44-45 0.9687764260384384 1105 46-47 1.1216369484278772 1105 48-49 1.204881613302895 1105 50-51 1.2353761675759145 1105 52-53 1.2513722764872668 1105 54-55 1.2815107591777917 1105 56-57 1.4088734635134657 1105 58-59 1.6223529711708764 1105 60-61 1.413783068332009 1105 62-63 1.533108563314105 1105 64-65 1.6602524379721189 1105 66-67 1.5672191618142222 1105 68-69 1.7879845036989828 1105 70-71 1.9034374879897342 1105 72-73 1.8356062745502584 1105 74-75 2.0908765626590124 1105 76-77 1.9058561520201494 1105 78-79 2.3456829816490483 1105 80-81 2.5359615129898394 1105 82-83 2.5228425391884812 1105 84-85 2.716628918658639 1105 86-87 2.82221896021165 1105 88-89 2.803047055881823 1105 90-91 2.968039684073343 1105 92-93 3.343816626500768 1105 94-95 3.5737144656567033 1105 96-97 3.837564730690186 1105 98-99 4.352156314938714 1105 100-101 4.60308453043816 1106 1 0.13945433023650722 1106 2 0.15325081159112486 1106 3 0.09694931086696101 1106 4 0.08776519725849141 1106 5 0.01848255426730816 1106 6 0.07355780219042174 1106 7 0.048552423948748924 1106 8 0.11278009887934104 1106 9 0.1717993615448492 1106 10-11 0.19973069502059104 1106 12-13 0.04496949137789841 1106 14-15 -0.030979085988015242 1106 16-17 0.022022862382875985 1106 18-19 0.10343868021582381 1106 20-21 0.30452819728058955 1106 22-23 0.06737283221411872 1106 24-25 0.019293728917155306 1106 26-27 -0.01771002733760696 1106 28-29 0.05983853363733971 1106 30-31 0.12472596423799587 1106 32-33 0.013896067635052134 1106 34-35 -0.04983481465612272 1106 36-37 0.13151631405072095 1106 38-39 0.09341330926297786 1106 40-41 0.14442138038423025 1106 42-43 0.5095293699388534 1106 44-45 -0.011812384019627586 1106 46-47 -0.14143020271174578 1106 48-49 0.3594543673192234 1106 50-51 0.122602794565708 1106 52-53 0.31260477348092053 1106 54-55 0.40122681379897074 1106 56-57 0.35124008058404144 1106 58-59 0.7260693432564906 1106 60-61 0.30901609896238824 1106 62-63 0.8054359173221606 1106 64-65 0.48876790880460774 1106 66-67 0.7705591470774493 1106 68-69 0.38329084761425847 1106 70-71 0.5983438205405243 1106 72-73 1.0960625277658202 1106 74-75 1.4012636716265163 1106 76-77 1.2629801712928348 1106 78-79 1.6066980402101194 1106 80-81 1.640961639605031 1106 82-83 1.885738049537263 1106 84-85 1.7798801559361266 1106 86-87 1.9971532901609592 1106 88-89 2.2928917453626916 1106 90-91 2.4191950940317213 1106 92-93 2.063446273973188 1106 94-95 2.5444981642903137 1106 96-97 3.1314183659976713 1106 98-99 3.6805078036554484 1106 100-101 3.9529996673771244 1107 1 0.20594318670647382 1107 2 0.1929780258669247 1107 3 0.12737330324878116 1107 4 0.07865437813414644 1107 5 -0.01736068131405233 1107 6 0.04261413966974459 1107 7 0.06353274528572683 1107 8 0.1776460136327387 1107 9 0.19963504957959088 1107 10-11 0.14669770281655303 1107 12-13 0.2610801180994784 1107 14-15 0.36760699720304046 1107 16-17 0.3610284749421453 1107 18-19 0.30766569600015004 1107 20-21 0.2255273765244823 1107 22-23 0.24347098928784305 1107 24-25 0.19878724689220206 1107 26-27 0.12228323247675377 1107 28-29 0.30480805362896035 1107 30-31 0.30267067036103157 1107 32-33 0.25910928765370755 1107 34-35 0.34655857716115435 1107 36-37 0.3873858162633539 1107 38-39 0.5015895441112619 1107 40-41 0.369436571678861 1107 42-43 0.35013311278159875 1107 44-45 0.4657150029315673 1107 46-47 0.2118983043158238 1107 48-49 0.43888931204605086 1107 50-51 0.4588506848328038 1107 52-53 0.6489815918697559 1107 54-55 0.6047630671081734 1107 56-57 0.7026361879593921 1107 58-59 0.795700727557886 1107 60-61 0.7108323871963478 1107 62-63 0.8253760898027203 1107 64-65 1.049959600414624 1107 66-67 1.2786399111843494 1107 68-69 1.3124012344412463 1107 70-71 1.311224546653964 1107 72-73 1.52993153435866 1107 74-75 1.4502934989267686 1107 76-77 1.4382542234656448 1107 78-79 1.8568729379809241 1107 80-81 1.9622549332725434 1107 82-83 2.1806791657655857 1107 84-85 2.207994127786872 1107 86-87 2.574503617854539 1107 88-89 2.8357528110791996 1107 90-91 2.898802074842589 1107 92-93 2.9477511702583534 1107 94-95 3.208141134161014 1107 96-97 3.652652538434392 1107 98-99 3.5759726694484257 1107 100-101 3.991569420687547 1108 1 0.17549871950994955 1108 2 0.12193339580782592 1108 3 0.24826780925612724 1108 4 0.19885302761293389 1108 5 0.02948195153561528 1108 6 -0.003354654528152423 1108 7 0.07488133785786033 1108 8 0.10928778264408123 1108 9 0.21516532916033526 1108 10-11 0.10073992089049 1108 12-13 0.20885397246605208 1108 14-15 0.2661869498681284 1108 16-17 0.36930761996126904 1108 18-19 0.3389250713126657 1108 20-21 0.31006590876125983 1108 22-23 0.225987668415236 1108 24-25 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2316 84-85 1.933554309930603 2316 86-87 2.063378712855247 2316 88-89 2.357317971904127 2316 90-91 2.6993899521081133 2316 92-93 2.3499138050305426 2316 94-95 3.134471627134179 2316 96-97 3.196477009328749 2316 98-99 3.5658843992986142 2316 100-101 3.8790618528345 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 14.0 4 30.0 5 62.0 6 128.0 7 375.0 8 839.0 9 1497.0 10 2432.0 11 3666.0 12 5312.0 13 7503.0 14 9764.0 15 13040.0 16 16305.0 17 19981.0 18 19587.0 19 17765.0 20 18264.0 21 19671.0 22 21591.0 23 24236.0 24 27748.0 25 31992.0 26 37801.0 27 44350.0 28 52918.0 29 61664.0 30 72233.0 31 85074.0 32 99957.0 33 119269.0 34 145156.0 35 179227.0 36 216437.0 37 221441.0 38 102630.0 39 4703.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.383057805424755 19.05140417453966 15.239105350194496 23.326432669841086 2 16.278088436907243 20.487245760324527 38.44066332375533 24.794002479012903 3 19.645138695791434 23.682117200671566 29.74348054105614 26.92926356248086 4 13.867283170261405 16.71493611362219 35.17836621463312 34.23941450148328 5 14.337699675445743 37.045751756803334 33.542362639911545 15.074185927839379 6 33.544968020147046 35.40656327599467 16.45113092469086 14.597337779167427 7 29.69365864418095 32.11336876938428 19.734528796533283 18.458443789901487 8 27.290725989298792 34.84727515696614 18.92199473562872 18.94000411810635 9 26.61868857671322 16.52023533041937 19.219648177620616 37.64142791524679 10-11 25.682435337810844 26.42038005076651 26.523977996875637 21.373206614547005 12-13 26.463731626176845 23.335026723694423 27.119695518244914 23.081546131883822 14-15 22.94873426892173 24.759938451322164 25.23721641822127 27.054110861534834 16-17 22.784684633421072 28.056623375709155 26.028719978740718 23.129972012129056 18-19 22.822023304258252 26.685945114207055 28.29681691497518 22.195214666559508 20-21 23.288126068023843 25.711238617278333 28.52533661999675 22.475298694701078 22-23 23.09307330991918 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27.5840545889928 22.876035540099938 48-49 22.977801529565596 26.453583016507036 27.535553864772417 23.03306158915495 50-51 23.174438174728554 26.35646727484019 27.509742371970596 22.959352178460662 52-53 23.433814346145024 26.20303554887846 27.319969237393575 23.043180867582937 54-55 23.191244976291557 26.512597475049375 27.23848704761693 23.05767050104214 56-57 23.03804790026439 26.58844806639655 27.183256319269393 23.19024771406967 58-59 23.100846089001546 26.253015984940163 27.35522539006152 23.290912535996767 60-61 22.798059679691107 26.424838399523193 27.56550106267089 23.21160085811481 62-63 22.84991731522931 26.5106029506056 27.718932788632618 22.92054694553247 64-65 22.814661162561368 26.566156322613153 27.278054892832444 23.341127621993035 66-67 22.58286677022569 26.614277065744336 27.27015411834825 23.53270204568173 68-69 23.028203798519826 26.320318842892203 27.350228549438484 23.301248809149484 70-71 23.05045501555436 26.308540025705902 26.955939195162458 23.685065763577285 72-73 22.863028380322962 26.469040580946306 26.99442178443062 23.673509254300107 74-75 22.663135967317956 26.758393281503075 26.915608737659614 23.662862013519355 76-77 22.92040028932337 26.453055054154273 27.069333776039546 23.557210880482813 78-79 22.79503856178362 26.637548565203645 27.158383426205823 23.409029446806915 80-81 22.85081065733698 26.45923013657808 27.097507664257986 23.59245154182696 82-83 22.915391966322684 26.40188684004742 27.184305977359564 23.49841521627033 84-85 22.756299977208588 26.602035270075287 27.004888860338518 23.636775892377607 86-87 22.96191136851275 26.546739272310766 26.779046046311763 23.712303312864723 88-89 22.790329601223934 26.634748390593817 26.61761893532223 23.95730307286002 90-91 22.62088991875165 26.5304901299387 26.918077024609154 23.930542926700497 92-93 22.74793133295457 26.325711189999417 26.750810498630077 24.175546978415937 94-95 22.73150709200096 25.976950336927974 27.05249764323472 24.239044927836346 96-97 22.63664985595427 25.992437232609067 27.0311738304314 24.339739081005263 98-99 22.39220328662431 26.17071252039255 26.762528986599726 24.674555206383417 100-101 22.58669784402249 25.720115964745965 27.081622333186285 24.61156385804526 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 421.0 1 305.0 2 246.5 3 342.5 4 321.0 5 289.0 6 552.0 7 1293.5 8 1961.0 9 2195.5 10 1923.5 11 1501.0 12 1509.0 13 1623.5 14 1718.5 15 1838.0 16 1941.0 17 2008.5 18 2134.0 19 2374.5 20 2662.0 21 3021.0 22 3577.5 23 4283.5 24 5073.5 25 6359.5 26 8114.0 27 10126.0 28 12007.5 29 13794.0 30 16065.5 31 19505.0 32 24254.5 33 26810.0 34 27462.5 35 29296.5 36 32250.0 37 35558.0 38 38505.0 39 40529.5 40 42575.5 41 45806.0 42 48133.0 43 50874.0 44 54677.0 45 57636.5 46 58968.0 47 61995.5 48 65349.0 49 66798.5 50 66562.0 51 65024.0 52 64651.5 53 64883.5 54 66075.0 55 63461.5 56 58438.5 57 54387.5 58 50046.0 59 44422.5 60 36283.5 61 29975.5 62 25283.5 63 18998.5 64 13538.0 65 10226.0 66 7881.0 67 6431.0 68 5251.0 69 4173.0 70 3235.0 71 2093.5 72 1477.5 73 1128.5 74 774.5 75 574.5 76 405.0 77 242.0 78 144.5 79 92.5 80 62.0 81 37.5 82 20.0 83 9.5 84 7.0 85 3.0 86 2.5 87 3.5 88 2.5 89 1.0 90 1.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0447594750177014 3 5.866248364049988E-5 4 0.0 5 0.10066482192709779 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0013492371237314971 26-27 0.011615171760818976 28-29 0.0 30-31 0.0 32-33 4.106373854834991E-4 34-35 0.014929602086507218 36-37 0.0029331241820249937 38-39 0.011615171760818976 40-41 0.006599529409556236 42-43 0.0048103236585209895 44-45 0.002434493071080745 46-47 5.866248364049988E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.7598745092149964E-4 68-69 1.7598745092149964E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 5.866248364049988E-5 82-83 0.0010559247055289978 84-85 0.00454634248213874 86-87 0.001437230849192247 88-89 5.866248364049988E-5 90-91 9.972622218884979E-4 92-93 3.813061436632492E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 6.452873200454986E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1704667.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.28276362058008 #Duplication Level Percentage of deduplicated Percentage of total 1 76.44630439705796 40.7327036685535 2 13.111601035863933 13.972446773625816 3 4.289799067956465 6.857170491531271 4 1.9168677169498733 4.085440378166444 5 1.0671493869300497 2.843033426582039 6 0.6502071664282281 2.0786900851921466 7 0.4577394060141538 1.7072734399333818 8 0.3203293560412474 1.3654426686942733 9 0.2448440950166016 1.1741373034797986 >10 1.33632919504179 12.95740952532611 >50 0.09874995118714337 3.5886968329325244 >100 0.054557557980672786 5.5023390961709 >500 0.004196467324145757 1.6087386717888679 >1k 0.001214766856989561 1.195609402782663 >5k 1.1043335063541463E-4 0.33086823524026354 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5623 0.3298591455105308 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4812 0.2822838712780854 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3540 0.20766519208736955 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.866248364049988E-5 0.0 10-11 0.0 0.0 0.0 5.866248364049988E-5 0.0 12-13 0.0 0.0 0.0 5.866248364049988E-5 5.866248364049988E-5 14-15 0.0 0.0 0.0 5.866248364049988E-5 5.866248364049988E-5 16-17 0.0 0.0 0.0 5.866248364049988E-5 1.1732496728099975E-4 18-19 0.0 0.0 0.0 5.866248364049988E-5 1.1732496728099975E-4 20-21 0.0 0.0 0.0 8.799372546074982E-5 1.1732496728099975E-4 22-23 0.0 0.0 0.0 1.7598745092149964E-4 1.1732496728099975E-4 24-25 0.0 0.0 0.0 3.813061436632492E-4 1.1732496728099975E-4 26-27 0.0 0.0 0.0 9.09268496427748E-4 1.1732496728099975E-4 28-29 0.0 0.0 0.0 0.003167774116586993 1.1732496728099975E-4 30-31 0.0 0.0 0.0 0.010529915813469728 1.1732496728099975E-4 32-33 0.0 0.0 0.0 0.025048880514493447 1.1732496728099975E-4 34-35 0.0 0.0 0.0 0.0467539994614784 1.1732496728099975E-4 36-37 0.0 0.0 0.0 0.08799372546074982 1.1732496728099975E-4 38-39 0.0 0.0 0.0 0.14956000204145442 1.1732496728099975E-4 40-41 0.0 0.0 0.0 0.21796045796627728 1.1732496728099975E-4 42-43 0.0 0.0 0.0 0.29122990003326166 1.7598745092149964E-4 44-45 0.0 0.0 0.0 0.3827727057542617 1.7598745092149964E-4 46-47 0.0 0.0 0.0 0.4908583318618827 1.7598745092149964E-4 48-49 0.0 0.0 0.0 0.6046928813662727 1.7598745092149964E-4 50-51 0.0 0.0 0.0 0.724657660411095 1.7598745092149964E-4 52-53 0.0 0.0 0.0 0.8436838397176691 1.7598745092149964E-4 54-55 0.0 0.0 0.0 0.9761143965360977 1.7598745092149964E-4 56-57 0.0 0.0 0.0 1.1415426004023073 1.7598745092149964E-4 58-59 0.0 0.0 0.0 1.3140103023053769 1.7598745092149964E-4 60-61 0.0 0.0 0.0 1.5001170316548629 1.7598745092149964E-4 62-63 0.0 0.0 0.0 1.6837306054496275 1.7598745092149964E-4 64-65 0.0 0.0 0.0 1.8835643559709903 1.7598745092149964E-4 66-67 0.0 0.0 0.0 2.115545147527347 1.7598745092149964E-4 68-69 0.0 0.0 0.0 2.374334694107412 1.7598745092149964E-4 70-71 0.0 0.0 0.0 2.644240781337352 1.7598745092149964E-4 72-73 0.0 0.0 0.0 2.916757349089294 1.7598745092149964E-4 74-75 0.0 0.0 0.0 3.2010944072947973 1.7598745092149964E-4 76-77 0.0 0.0 0.0 3.5155253196078764 1.7598745092149964E-4 78-79 0.0 0.0 0.0 3.871899907723913 1.7598745092149964E-4 80-81 0.0 0.0 0.0 4.244113366422885 2.0531869274174956E-4 82-83 0.0 0.0 0.0 4.6327816517830165 2.346499345619995E-4 84-85 0.0 0.0 0.0 5.025673635965265 2.346499345619995E-4 86-87 0.0 0.0 0.0 5.436281690206943 2.6398117638224946E-4 88-89 0.0 0.0 0.0 5.877951529536267 2.933124182024994E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4040 0.0 40.15133 1 GTATCAA 6450 0.0 37.02294 1 TATCAAC 8250 0.0 28.599253 2 ATCAACG 8235 0.0 28.579306 3 TCAACGC 8365 0.0 28.078318 4 CAACGCA 8595 0.0 27.32695 5 TATACGA 70 0.008353523 27.168896 5 TAAGGTG 785 0.0 26.649746 5 AACGCAG 8830 0.0 26.625378 6 TACATGG 6305 0.0 24.218477 2 ACGCAGA 9925 0.0 23.640013 7 CGCAGAG 10000 0.0 23.36772 8 GTACATG 6605 0.0 23.262512 1 GAGTACT 5405 0.0 22.846985 12-13 ACATGGG 6280 0.0 22.485723 3 AGGTAAG 800 0.0 22.000809 2 GTACTTT 5640 0.0 21.473969 14-15 GTAAGGT 895 0.0 21.249416 4 AGAGTAC 8515 0.0 21.223705 10-11 GGACCGA 575 0.0 20.650158 6 >>END_MODULE