FastQCFastQC Report
Thu 26 May 2016
SRR938496_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938496_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1695777
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56720.334477941380264No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44510.26247554955633906No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31550.18605040639187817No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34100.046.6018451
GTATCAA60000.040.0859831
TATCAAC78100.030.4252452
ATCAACG78300.030.347533
TCAACGC80100.029.6655624
CAACGCA82000.028.7463635
AACGCAG84450.028.0199836
TACATGG66400.026.8397372
GTACATG70000.026.723991
TATACCG1257.3643787E-626.6135675
ACATGGG67050.025.6581233
ACGCAGA95900.024.4712897
CGCAGAG95550.024.2128988
CATGGGG42350.022.3313394
GCAGAGT104500.021.9553789
GAGTACT57000.021.91685112-13
GGACCGA4950.021.1181436
GTACTTT58800.021.044914-15
AGAGTAC86650.020.99524910-11
TAAGGTA5250.020.8201394
Produced by FastQC (version 0.11.5)