##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938496_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1695777 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.129594280380026 31.0 31.0 34.0 28.0 34.0 2 31.310140425303562 31.0 31.0 34.0 28.0 34.0 3 31.366397232655 31.0 31.0 34.0 28.0 34.0 4 35.051149414103385 37.0 35.0 37.0 32.0 37.0 5 35.03749903436596 37.0 35.0 37.0 32.0 37.0 6 35.01281123638309 37.0 35.0 37.0 32.0 37.0 7 34.9276667863758 37.0 35.0 37.0 32.0 37.0 8 34.88363328432925 37.0 35.0 37.0 32.0 37.0 9 36.37221580431861 39.0 35.0 39.0 32.0 39.0 10-11 36.20050307322248 39.0 35.0 39.0 32.0 39.0 12-13 36.01478761653213 38.0 35.0 39.0 30.5 39.0 14-15 37.17126514866047 39.0 36.0 41.0 31.5 41.0 16-17 37.102224231134166 39.0 36.0 41.0 31.0 41.0 18-19 37.08382293190673 39.0 36.0 41.0 31.0 41.0 20-21 37.010577746956116 39.0 36.0 41.0 31.0 41.0 22-23 36.85713156859658 39.0 36.0 41.0 30.0 41.0 24-25 36.704088450309214 39.0 36.0 41.0 30.0 41.0 26-27 36.485503105655994 39.0 36.0 41.0 30.0 41.0 28-29 36.24108653437332 39.0 35.0 40.0 29.5 41.0 30-31 35.95695100240185 38.5 35.0 40.0 28.5 41.0 32-33 35.65509232640848 38.0 34.5 40.0 27.0 41.0 34-35 35.33897145674224 38.0 34.0 40.0 26.5 41.0 36-37 35.11187644365975 38.0 34.0 40.0 25.5 41.0 38-39 34.858839340314205 38.0 34.0 40.0 25.0 41.0 40-41 34.56620534421684 38.0 33.0 40.0 24.0 41.0 42-43 34.13050330320555 38.0 33.0 40.0 23.5 41.0 44-45 33.6416654430388 37.5 32.5 40.0 21.5 41.0 46-47 33.1367423310966 37.0 31.5 40.0 18.5 41.0 48-49 32.61453097901434 37.0 31.0 39.5 15.5 41.0 50-51 31.877078766842576 36.0 30.5 38.5 13.0 40.0 52-53 31.883714368103824 36.0 30.5 39.0 11.0 40.0 54-55 31.84469007422556 36.0 30.5 39.0 8.5 40.0 56-57 31.38661923118429 36.0 30.0 39.0 7.0 40.0 58-59 30.896323337325605 35.0 29.0 39.0 2.0 40.0 60-61 30.32439908077536 35.0 28.0 38.5 2.0 40.0 62-63 29.57109218959804 34.0 27.5 38.0 2.0 40.0 64-65 28.87924444074899 34.0 26.0 38.0 2.0 40.0 66-67 28.148457019997323 33.5 25.5 37.0 2.0 39.0 68-69 27.27802299476877 33.0 23.0 36.0 2.0 39.0 70-71 26.41042896560102 32.0 21.0 36.0 2.0 39.0 72-73 25.479782129371962 31.5 18.5 35.0 2.0 38.0 74-75 24.512726614407434 31.0 10.5 35.0 2.0 37.0 76-77 23.557798873318838 30.0 4.5 34.0 2.0 36.5 78-79 22.549364391662348 29.0 2.0 34.0 2.0 36.0 80-81 21.573638514969833 28.5 2.0 34.0 2.0 35.0 82-83 20.55739080079515 26.5 2.0 33.5 2.0 35.0 84-85 19.55121339657278 26.0 2.0 33.0 2.0 35.0 86-87 18.533899799325027 24.5 2.0 32.0 2.0 34.5 88-89 17.422735418631106 21.5 2.0 32.0 2.0 34.0 90-91 16.23665994998163 14.5 2.0 31.0 2.0 34.0 92-93 15.046656193591492 4.5 2.0 31.0 2.0 34.0 94-95 13.763043135978375 2.0 2.0 30.0 2.0 34.0 96-97 12.34768221293248 2.0 2.0 29.0 2.0 34.0 98-99 10.68794629246652 2.0 2.0 26.0 2.0 33.5 100-101 7.575296751872446 2.0 2.0 2.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5889604259663059 1101 2 0.4479630438265616 1101 3 0.4776701214564092 1101 4 0.36128512208480856 1101 5 0.42094719373912426 1101 6 0.43014763404435996 1101 7 0.43125855543141967 1101 8 0.4739533532026954 1101 9 0.40019937887041124 1101 10-11 0.5420172273105806 1101 12-13 0.48444065995843033 1101 14-15 0.6292239589463478 1101 16-17 0.6787290741428151 1101 18-19 0.6219505778052934 1101 20-21 0.7379107447973681 1101 22-23 0.7025098605955051 1101 24-25 0.8824764778686642 1101 26-27 0.8420666129282282 1101 28-29 0.8070270817256002 1101 30-31 0.9311389628139111 1101 32-33 0.9643444136276642 1101 34-35 1.064450030224073 1101 36-37 1.1556053507825652 1101 38-39 1.1850172279350843 1101 40-41 1.1213639392466845 1101 42-43 1.168135877985101 1101 44-45 1.4732754142509137 1101 46-47 1.5547570938432216 1101 48-49 1.0383578530045199 1101 50-51 1.3568967418144773 1101 52-53 1.2980993028557002 1101 54-55 1.3699149776917672 1101 56-57 1.7497957377116045 1101 58-59 1.5060254784425666 1101 60-61 1.5847782580187797 1101 62-63 1.6643323030816681 1101 64-65 1.4444362886558046 1101 66-67 1.9285496631633592 1101 68-69 1.8777001733296643 1101 70-71 2.097328203563233 1101 72-73 2.4149584446541112 1101 74-75 2.4368398241285902 1101 76-77 2.4814541812677753 1101 78-79 2.5291930901869044 1101 80-81 2.9786732803694775 1101 82-83 3.0039083772517117 1101 84-85 3.11707642571594 1101 86-87 3.4442072007903377 1101 88-89 3.539681143410096 1101 90-91 3.3147434044080484 1101 92-93 3.4283334653196533 1101 94-95 3.288056993476305 1101 96-97 3.5495148381197215 1101 98-99 3.2450073185441504 1101 100-101 2.7796427215879227 1102 1 0.5628495252250758 1102 2 0.508398311276224 1102 3 0.4561046009752481 1102 4 0.2991839338253257 1102 5 0.3236818978495464 1102 6 0.41706784335717373 1102 7 0.3559576134959386 1102 8 0.577004690059816 1102 9 0.5497417635329498 1102 10-11 0.5752920047966228 1102 12-13 0.5998078492812411 1102 14-15 0.6424437064538111 1102 16-17 0.7344574459460134 1102 18-19 0.7228255426422265 1102 20-21 0.7148882825100813 1102 22-23 0.5979304734359872 1102 24-25 0.48303015052687215 1102 26-27 0.6722541342042732 1102 28-29 0.4338547352460651 1102 30-31 0.4982196582654339 1102 32-33 0.5451419206228962 1102 34-35 0.5872469968642804 1102 36-37 0.7619866168058849 1102 38-39 0.9101729798822262 1102 40-41 1.1656469726203937 1102 42-43 1.143022234785633 1102 44-45 0.9564207881825055 1102 46-47 0.8758303835795687 1102 48-49 1.0519891257214269 1102 50-51 1.1381520553925135 1102 52-53 0.768499217734135 1102 54-55 0.9582717177166735 1102 56-57 1.2278423368999576 1102 58-59 1.4793502300757169 1102 60-61 1.24610396379234 1102 62-63 1.3025653073686918 1102 64-65 1.67773439807387 1102 66-67 1.7329571381441191 1102 68-69 1.6484227364901791 1102 70-71 1.736067834153726 1102 72-73 1.7961634433206086 1102 74-75 1.5544033793030465 1102 76-77 1.8304665033791743 1102 78-79 2.448516937055718 1102 80-81 2.6011042034203484 1102 82-83 2.766654966390991 1102 84-85 2.8761083348278085 1102 86-87 2.9223395126649017 1102 88-89 2.931276153923374 1102 90-91 3.0602160185962965 1102 92-93 3.038962116584461 1102 94-95 3.0051635765930964 1102 96-97 2.631993739411742 1102 98-99 2.75644361628464 1102 100-101 2.2503197683564515 1103 1 0.5411316978340253 1103 2 0.45299885535625606 1103 3 0.46822788785793534 1103 4 0.31451894531439706 1103 5 0.21038945517129548 1103 6 0.4697059271985893 1103 7 0.4387760889205765 1103 8 0.24491473603640657 1103 9 0.3896316201718619 1103 10-11 0.5999014001661891 1103 12-13 0.5343878803115487 1103 14-15 0.563754929848308 1103 16-17 0.6214375850609244 1103 18-19 0.5786517837697716 1103 20-21 0.6303298548566758 1103 22-23 0.7739878368352677 1103 24-25 0.9080412061735075 1103 26-27 0.9121513387002054 1103 28-29 0.7864110106884397 1103 30-31 0.9242746513882523 1103 32-33 1.1235486343293957 1103 34-35 1.0677015278509074 1103 36-37 1.0235461165014783 1103 38-39 1.0686461087351873 1103 40-41 1.1896373872223762 1103 42-43 1.415875085991189 1103 44-45 1.3007569448537168 1103 46-47 1.6942155684035711 1103 48-49 1.326514545582043 1103 50-51 1.571504283215365 1103 52-53 1.7036743574619244 1103 54-55 1.8012789958198603 1103 56-57 1.8329598653652859 1103 58-59 2.0744257436977023 1103 60-61 1.9678810819560368 1103 62-63 2.1828385799281413 1103 64-65 2.2801752071914443 1103 66-67 2.6295409602623216 1103 68-69 2.7074344937386527 1103 70-71 2.8708730753090386 1103 72-73 3.0465904316947388 1103 74-75 2.85156155326915 1103 76-77 2.994789305235578 1103 78-79 3.16530682423733 1103 80-81 3.1122353602981114 1103 82-83 3.3803225899721276 1103 84-85 3.341109848418636 1103 86-87 3.708081080745 1103 88-89 3.9180638943117323 1103 90-91 3.8161737084842215 1103 92-93 3.5718671409610607 1103 94-95 3.635380470433832 1103 96-97 3.652024776488961 1103 98-99 3.192926471397767 1103 100-101 2.4414236467382437 1104 1 0.3480557183599693 1104 2 0.2856708565445025 1104 3 0.18229885571334847 1104 4 0.28603486712194126 1104 5 0.29026070223894607 1104 6 0.3463395240007472 1104 7 0.2881425194794005 1104 8 0.2832591885660918 1104 9 0.4052019694737936 1104 10-11 0.40799037936076843 1104 12-13 0.42724067240136065 1104 14-15 0.3980327597060338 1104 16-17 0.5531100637633344 1104 18-19 0.5448486016883507 1104 20-21 0.48542759542642244 1104 22-23 0.5021132256705556 1104 24-25 0.6036280437203274 1104 26-27 0.7291925684327296 1104 28-29 0.6266167261356017 1104 30-31 0.6588528330950894 1104 32-33 0.8899735255206593 1104 34-35 0.8717348939088296 1104 36-37 0.9206732466674055 1104 38-39 0.8785754392446989 1104 40-41 1.028178480475745 1104 42-43 1.0925121680604306 1104 44-45 0.9257956248039179 1104 46-47 0.8886838632219849 1104 48-49 1.2139042629366514 1104 50-51 1.2282803916117295 1104 52-53 1.201403233648957 1104 54-55 1.3393672649319832 1104 56-57 1.4921119813132826 1104 58-59 1.8368758017746245 1104 60-61 1.8855051656076611 1104 62-63 1.943838506314087 1104 64-65 1.9706582456668365 1104 66-67 2.2329524178661657 1104 68-69 2.2041085066100408 1104 70-71 2.5416148443785005 1104 72-73 2.7626533762698706 1104 74-75 2.5397382895347533 1104 76-77 2.7636802671888354 1104 78-79 2.8884935570306958 1104 80-81 3.1148779744959825 1104 82-83 3.0603838655277684 1104 84-85 3.3040116231687513 1104 86-87 3.267626054696695 1104 88-89 3.3490204274937554 1104 90-91 3.0193647799465317 1104 92-93 3.088152621468648 1104 94-95 3.2634409095253663 1104 96-97 3.3087671722550454 1104 98-99 2.4281430758616445 1104 100-101 1.4338567084378937 1105 1 0.5520430847443087 1105 2 0.4872879666921186 1105 3 0.36257876440325987 1105 4 0.28058881561636895 1105 5 0.3559431429756543 1105 6 0.3686560968556023 1105 7 0.4631005955913352 1105 8 0.5334615032603622 1105 9 0.3958953405582619 1105 10-11 0.5946011699469338 1105 12-13 0.5867218656251225 1105 14-15 0.6794065292578964 1105 16-17 0.5873788839252327 1105 18-19 0.4860606173913311 1105 20-21 0.682002365583152 1105 22-23 0.6598109900052194 1105 24-25 0.8495750952494632 1105 26-27 0.60266763576643 1105 28-29 0.6779307696360917 1105 30-31 0.7459656394852132 1105 32-33 0.9014564333327684 1105 34-35 0.9028307771528361 1105 36-37 0.7373752157821798 1105 38-39 0.8179084141992874 1105 40-41 0.8650955502675117 1105 42-43 1.0275319008184027 1105 44-45 1.2245198107492499 1105 46-47 1.3261134737711728 1105 48-49 1.2472795329062905 1105 50-51 1.2396260772833791 1105 52-53 1.3043669565208127 1105 54-55 1.4806799102046462 1105 56-57 1.8357644094108672 1105 58-59 1.8143034480737725 1105 60-61 2.1477224489886666 1105 62-63 2.1866314373392335 1105 64-65 2.327398636980938 1105 66-67 2.345909186313822 1105 68-69 2.565567687383151 1105 70-71 2.7333371214521804 1105 72-73 2.9212633542317192 1105 74-75 3.106917476561648 1105 76-77 3.0900492836839497 1105 78-79 3.23039070437056 1105 80-81 3.4471588634751633 1105 82-83 3.4484988677780883 1105 84-85 3.39087813340932 1105 86-87 3.544296026729082 1105 88-89 3.5369912011665647 1105 90-91 3.0605526861630743 1105 92-93 3.281503665687829 1105 94-95 3.1930320303809 1105 96-97 3.163723981701068 1105 98-99 2.7206153170617586 1105 100-101 1.9018720173498682 1106 1 0.2075962245440941 1106 2 0.26971656560832 1106 3 0.22617838347957075 1106 4 0.3724852831681744 1106 5 0.2975217319662562 1106 6 -0.15626449067924142 1106 7 0.22774879158590267 1106 8 -0.2321085085436323 1106 9 0.20011697032739306 1106 10-11 0.1848058630658187 1106 12-13 0.37809646894396565 1106 14-15 0.5988639782950216 1106 16-17 0.11060967909885733 1106 18-19 0.4442969943838051 1106 20-21 0.44917831286858956 1106 22-23 0.2744364836554283 1106 24-25 0.40633995171545223 1106 26-27 0.4811713667877129 1106 28-29 0.4758600840801819 1106 30-31 0.2864386288792957 1106 32-33 0.3002995743126604 1106 34-35 0.18002701170448887 1106 36-37 0.3455993801636126 1106 38-39 0.3535607002750467 1106 40-41 0.7537956680025673 1106 42-43 0.17044628416388008 1106 44-45 0.49621762592686025 1106 46-47 0.5855652876259825 1106 48-49 0.2915509340205844 1106 50-51 0.26925332278003467 1106 52-53 0.47224385584101825 1106 54-55 0.35023977136204465 1106 56-57 0.783446559513461 1106 58-59 0.9257304878618804 1106 60-61 0.9923568436654939 1106 62-63 1.0176229275430693 1106 64-65 1.0304923764702387 1106 66-67 1.1978235778925175 1106 68-69 1.4402338226513152 1106 70-71 1.290133691065435 1106 72-73 1.3414596586132888 1106 74-75 1.699516054897849 1106 76-77 1.8744227523429728 1106 78-79 2.1549129564797163 1106 80-81 2.1319875072776675 1106 82-83 2.4756386132861827 1106 84-85 2.3214109417274322 1106 86-87 2.1109078154207204 1106 88-89 1.9149353780990914 1106 90-91 2.1670687621368003 1106 92-93 2.3834788153876083 1106 94-95 2.3541685137038986 1106 96-97 2.1631784545812955 1106 98-99 1.8713692513978568 1106 100-101 1.46453155506366 1107 1 0.16756109721201895 1107 2 0.11600445415712812 1107 3 0.18941782267964413 1107 4 0.10333801897645856 1107 5 0.07587655586257114 1107 6 0.054845188828906544 1107 7 0.21113508922336877 1107 8 0.28471317669733054 1107 9 0.1940892210565366 1107 10-11 0.28315743732352416 1107 12-13 0.1442493803588789 1107 14-15 0.2753731155760377 1107 16-17 0.3169564756069079 1107 18-19 0.43488340398664604 1107 20-21 0.44157124523496094 1107 22-23 0.44048085353637845 1107 24-25 0.4109670827140306 1107 26-27 0.53370333683457 1107 28-29 0.49176964801892353 1107 30-31 0.43207965313415286 1107 32-33 0.5478466527764141 1107 34-35 0.6135894715908208 1107 36-37 0.6394381914806502 1107 38-39 0.67730099228001 1107 40-41 0.544352704716573 1107 42-43 0.6887705518464031 1107 44-45 0.5223371219265331 1107 46-47 0.38146680855896875 1107 48-49 0.276768532165228 1107 50-51 0.5746735518104806 1107 52-53 0.6228003339295363 1107 54-55 0.7956236176547371 1107 56-57 0.9164884765479719 1107 58-59 0.7893216508871177 1107 60-61 0.8549858793967999 1107 62-63 1.2930291999462789 1107 64-65 1.3518987551924937 1107 66-67 1.4829954348207188 1107 68-69 1.4563153291140978 1107 70-71 1.6455100559812337 1107 72-73 1.6939921392318382 1107 74-75 1.7353680984309925 1107 76-77 1.7018681429468323 1107 78-79 2.2164686804238123 1107 80-81 2.2230647865072193 1107 82-83 2.371784277586496 1107 84-85 2.2781444962884727 1107 86-87 2.191454253267178 1107 88-89 2.3458778543897942 1107 90-91 2.6525838433732574 1107 92-93 2.573558347439647 1107 94-95 2.4468210567563577 1107 96-97 2.487923521632874 1107 98-99 2.2057698297254102 1107 100-101 1.4002002733274992 1108 1 0.30364313195137527 1108 2 0.29901816132169756 1108 3 0.14784781686196524 1108 4 0.2476888287066643 1108 5 0.13096076509246046 1108 6 0.16855699715761574 1108 7 0.02828924961302448 1108 8 0.21788314201912584 1108 9 0.2793858404289651 1108 10-11 0.22966623926546959 1108 12-13 0.1423589955643294 1108 14-15 0.21246143265765483 1108 16-17 0.2507305622213707 1108 18-19 0.26685158760490424 1108 20-21 0.3413821490690623 1108 22-23 0.36116054984032786 1108 24-25 0.3076091259217577 1108 26-27 0.228642562722861 1108 28-29 0.24860094891458573 1108 30-31 0.4090676650256384 1108 32-33 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0.05654814778182882 2316 92-93 0.11185984131464721 2316 94-95 0.027097067767423155 2316 96-97 0.09629441837210528 2316 98-99 0.22039037342511314 2316 100-101 0.36567489025607536 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4922.0 3 1862.0 4 2065.0 5 2381.0 6 3021.0 7 3836.0 8 5012.0 9 6710.0 10 8366.0 11 10792.0 12 13349.0 13 16010.0 14 18522.0 15 21288.0 16 24971.0 17 28906.0 18 29005.0 19 29296.0 20 31802.0 21 35162.0 22 39512.0 23 43931.0 24 48659.0 25 53953.0 26 59926.0 27 65667.0 28 72726.0 29 80211.0 30 88309.0 31 98853.0 32 109905.0 33 122281.0 34 134485.0 35 142587.0 36 133005.0 37 85263.0 38 19022.0 39 204.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.83064088881154 18.034317198792202 14.451474732694155 23.6835671797021 2 15.67164003024837 19.618438764158277 39.739444038747166 24.970477166846184 3 19.503871798547316 23.451885226995877 29.195018661437803 27.849224313019 4 13.233647462210731 15.902169719266244 35.63164210310208 35.232540715420946 5 14.43809530553166 36.81827891129969 33.39298927609195 15.350636507076693 6 33.57956483106199 34.84490365700975 16.822882064946114 14.752649446982149 7 29.70058961811977 32.10377750838414 19.757899673483113 18.43773320001298 8 27.323326979812084 34.37827225516968 19.13902412981241 19.15937663520582 9 26.963921235503012 16.557179768520157 19.183980462251796 37.29491853372503 10-11 25.704514283186104 26.206586340643295 26.57964516937779 21.509254206792807 12-13 26.57826553078285 23.284782509316184 27.00178876631162 23.135163193589346 14-15 23.172702335157663 24.576592858771285 25.26999993216638 26.98070487390467 16-17 22.96445718465041 27.903675785351123 25.93248280662019 23.199384223378278 18-19 22.958957212430185 26.530245694006606 28.228282165146172 22.282514928417037 20-21 23.44931225233489 25.607455134548424 28.458315574936993 22.48491703817969 22-23 23.148445279207937 25.432391235612524 28.523339688442352 22.895823796737186 24-25 23.13863363916105 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23.20352557911571 50-51 23.168599919320023 26.346641990860988 27.36230735296546 23.12245073685353 52-53 23.407513388119376 26.136091881083484 27.322880363098832 23.13351436769831 54-55 23.133595791779296 26.52471775298141 27.228496016385616 23.113190438853678 56-57 22.947159338203118 26.62140707915543 27.1427779474955 23.288655635145957 58-59 23.06927706491487 26.13251534538279 27.350179615505173 23.44802797419717 60-61 22.77278376780483 26.323945053276265 27.560290568324397 23.34298061059451 62-63 22.827063917725198 26.39503392927283 27.760726969908927 23.01717518309304 64-65 22.826475417183676 26.43755573064348 27.374066438003936 23.36190241416891 66-67 22.62321638764982 26.51915179192742 27.278506542369126 23.57912527805364 68-69 23.033865404706166 26.172598004857672 27.398456714859066 23.395079875577096 70-71 23.05168734284234 26.086070571180276 27.073743896156437 23.78849818982095 72-73 22.857430157647514 26.311633172356586 27.04816298077391 23.78277368922199 74-75 22.733580564077705 26.547580712699236 27.019542552350757 23.6992961708723 76-77 23.0928981084229 26.26190160260663 26.919014551727187 23.72618573724328 78-79 22.921475608418366 26.433718284574546 26.973607387478133 23.671198719528952 80-81 23.06495531620135 26.21467213893055 26.85672344759702 23.863649097271082 82-83 22.99634573283509 26.24926867474427 26.954850101913713 23.799535490506926 84-85 22.936461271623802 26.30792566595681 26.768724312841798 23.986888749577595 86-87 23.1882592227187 26.230604972132966 26.50390893366931 24.077226871479027 88-89 23.136685114448102 26.235434662149633 26.38011119391167 24.2477690294906 90-91 22.92577977538694 26.18045724801812 26.475881228765573 24.41788174782937 92-93 23.1616403568188 25.82041284045212 26.463461635432296 24.55448516729678 94-95 23.114347266397413 25.53500518305855 26.67834513714093 24.67230241340311 96-97 23.084944468085922 25.51365656590679 26.61090620192142 24.790492764085872 98-99 22.941094770254065 25.595467508306157 26.191841786244762 25.27159593519502 100-101 23.889558870199938 24.49915493789347 26.333434199552748 25.27785199235385 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 322.0 1 258.0 2 238.5 3 304.5 4 277.5 5 262.0 6 460.5 7 1036.5 8 1672.0 9 1895.5 10 1682.0 11 1406.5 12 1378.0 13 1466.5 14 1606.5 15 1776.5 16 1881.5 17 1991.5 18 2164.5 19 2346.5 20 2610.0 21 3050.5 22 3631.0 23 4260.5 24 5175.0 25 6400.0 26 7961.5 27 9949.0 28 11820.5 29 13762.5 30 16103.0 31 19475.0 32 23585.0 33 26035.0 34 27244.5 35 29127.0 36 32100.0 37 35150.0 38 37748.0 39 39676.5 40 41698.0 41 44646.5 42 47159.5 43 49798.5 44 53093.5 45 56121.0 46 57755.0 47 60240.0 48 63435.5 49 64972.5 50 64714.0 51 63879.0 52 63647.5 53 63652.0 54 64032.0 55 62005.5 56 58049.0 57 54110.5 58 50528.0 59 45669.5 60 38364.5 61 32129.0 62 26966.5 63 21054.5 64 15719.0 65 12084.5 66 9483.0 67 7582.5 68 6015.5 69 4792.0 70 3892.0 71 2784.5 72 1935.0 73 1397.0 74 1000.5 75 735.0 76 498.0 77 351.5 78 246.5 79 152.5 80 89.0 81 54.0 82 34.0 83 22.5 84 14.5 85 9.5 86 6.5 87 5.5 88 4.0 89 4.0 90 3.0 91 3.0 92 3.5 93 1.0 94 0.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.37858751474987573 2 0.02824663856155615 3 0.03361291018807308 4 0.042399442851271125 5 0.062449248928367354 6 0.052778165997062115 7 0.0360896509387732 8 0.038566391689473324 9 0.03520510067066601 10-11 0.03549995076003507 12-13 0.02798127348112399 14-15 0.026683933087900118 16-17 0.021052296380951033 18-19 0.02574041280191912 20-21 0.03358342517913617 22-23 0.03261041988421826 24-25 0.04157386260103776 26-27 0.03614862095664701 28-29 0.030605439276508644 30-31 0.03396673029531595 32-33 0.028099213516871616 34-35 0.02983882904414908 36-37 0.03210917473229086 38-39 0.014978384539948352 40-41 0.013120828976923262 42-43 0.013710529155661386 44-45 0.011145333378150546 46-47 0.019637015951979534 48-49 0.016659030049352007 50-51 0.011145333378150546 52-53 0.008874987690008768 54-55 0.008462197564892082 56-57 0.008904472698945676 58-59 0.004216356277977588 60-61 0.00793146740402777 62-63 0.011263273413898172 64-65 0.007135372162731302 66-67 0.0035971710903025572 68-69 0.006398246939308648 70-71 0.013356709048418514 72-73 0.011233788404961266 74-75 0.011499153485393421 76-77 0.0060149418231288665 78-79 0.008904472698945676 80-81 0.011558123503267233 82-83 0.013268254021607794 84-85 0.007901982395090865 86-87 0.002712620822195371 88-89 0.01595138983486626 90-91 0.024590497453379774 92-93 0.010762028261970766 94-95 0.02061002124689744 96-97 0.018310190549818754 98-99 0.011646578530077953 100-101 0.12469210279417635 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1695777.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.22661942967857 #Duplication Level Percentage of deduplicated Percentage of total 1 78.77842803525826 44.29444692406786 2 11.87721866907358 13.356317079781471 3 3.884686255553259 6.552683270840884 4 1.7299957007841822 3.8908723953188917 5 0.9133403289490548 2.567701954279797 6 0.6145964500579314 2.0734008420143257 7 0.3824347474147958 1.505210909970388 8 0.2735506398456344 1.2304662177076442 9 0.21552127996235204 1.090622968869634 >10 1.1907490629395545 12.109582293875459 >50 0.08488366321166052 3.248310879488378 >100 0.04942109752614396 5.112283395228091 >500 0.0038013571275988638 1.4392249390841163 >1k 0.0012671190425329545 1.192120686104945 >5k 1.0559325354441289E-4 0.33675524336805857 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5672 0.334477941380264 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4451 0.26247554955633906 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3155 0.18605040639187817 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 8.845502681071862E-5 2.9485008936906208E-5 22-23 0.0 0.0 0.0 1.4742504468453105E-4 5.8970017873812415E-5 24-25 0.0 0.0 0.0 2.3588007149524966E-4 5.8970017873812415E-5 26-27 0.0 0.0 0.0 9.140352770440924E-4 5.8970017873812415E-5 28-29 0.0 0.0 0.0 0.0036266560992394637 5.8970017873812415E-5 30-31 0.0 0.0 0.0 0.011145333378150548 5.8970017873812415E-5 32-33 0.0 0.0 0.0 0.026035262891288182 5.8970017873812415E-5 34-35 0.0 0.0 0.0 0.050301425246362 5.8970017873812415E-5 36-37 0.0 0.0 0.0 0.08801275167666503 5.8970017873812415E-5 38-39 0.0 0.0 0.0 0.14875187008669183 5.8970017873812415E-5 40-41 0.0 0.0 0.0 0.21574181039134271 5.8970017873812415E-5 42-43 0.0 0.0 0.0 0.28234844557981387 5.8970017873812415E-5 44-45 0.0 0.0 0.0 0.36950613199730864 5.8970017873812415E-5 46-47 0.0 0.0 0.0 0.4783942700013033 5.8970017873812415E-5 48-49 0.0 0.0 0.0 0.5896706937291873 5.8970017873812415E-5 50-51 0.0 0.0 0.0 0.7105887153794397 5.8970017873812415E-5 52-53 0.0 0.0 0.0 0.8289710262611181 8.845502681071862E-5 54-55 0.0 0.0 0.0 0.9607985012180258 1.1794003574762483E-4 56-57 0.0 0.0 0.0 1.119840639423698 1.1794003574762483E-4 58-59 0.0 0.0 0.0 1.2924458817403468 1.1794003574762483E-4 60-61 0.0 0.0 0.0 1.4782309230517927 1.1794003574762483E-4 62-63 0.0 0.0 0.0 1.6646646345598508 1.1794003574762483E-4 64-65 0.0 0.0 0.0 1.8583221732574506 1.1794003574762483E-4 66-67 0.0 0.0 0.0 2.08105193076684 1.1794003574762483E-4 68-69 0.0 0.0 0.0 2.3350357977493506 1.4742504468453105E-4 70-71 0.0 0.0 0.0 2.6047056894862948 1.7691005362143725E-4 72-73 0.0 0.0 0.0 2.880891768198295 2.0639506255834344E-4 74-75 0.0 0.0 0.0 3.164124764046216 2.3588007149524966E-4 76-77 0.0 5.8970017873812415E-5 0.0 3.4647539151669116 2.3588007149524966E-4 78-79 5.8970017873812415E-5 5.8970017873812415E-5 0.0 3.8015022022353175 2.3588007149524966E-4 80-81 5.8970017873812415E-5 5.8970017873812415E-5 0.0 4.1586246304791255 2.3588007149524966E-4 82-83 5.8970017873812415E-5 5.8970017873812415E-5 0.0 4.528189732494308 2.3588007149524966E-4 84-85 5.8970017873812415E-5 5.8970017873812415E-5 0.0 4.899052174902714 2.3588007149524966E-4 86-87 5.8970017873812415E-5 5.8970017873812415E-5 0.0 5.286662102387283 2.3588007149524966E-4 88-89 5.8970017873812415E-5 5.8970017873812415E-5 0.0 5.6904592997782135 2.3588007149524966E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3410 0.0 46.601845 1 GTATCAA 6000 0.0 40.085983 1 TATCAAC 7810 0.0 30.425245 2 ATCAACG 7830 0.0 30.34753 3 TCAACGC 8010 0.0 29.665562 4 CAACGCA 8200 0.0 28.746363 5 AACGCAG 8445 0.0 28.019983 6 TACATGG 6640 0.0 26.839737 2 GTACATG 7000 0.0 26.72399 1 TATACCG 125 7.3643787E-6 26.613567 5 ACATGGG 6705 0.0 25.658123 3 ACGCAGA 9590 0.0 24.471289 7 CGCAGAG 9555 0.0 24.212898 8 CATGGGG 4235 0.0 22.331339 4 GCAGAGT 10450 0.0 21.955378 9 GAGTACT 5700 0.0 21.916851 12-13 GGACCGA 495 0.0 21.118143 6 GTACTTT 5880 0.0 21.0449 14-15 AGAGTAC 8665 0.0 20.995249 10-11 TAAGGTA 525 0.0 20.820139 4 >>END_MODULE