FastQCFastQC Report
Thu 26 May 2016
SRR938496_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938496_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1695777
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56590.33371133114790447No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47300.27892818454313273No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33660.19849308016325262No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35950.048.501511
GTATCAA61700.038.1930731
ATCAACG79800.029.2247923
TATCAAC80800.028.9244442
TCAACGC81550.028.5968074
CAACGCA82650.028.2162115
AACGCAG85000.027.4361156
GTGTACG2551.8189894E-1124.2209571
ACGCAGA97100.023.7726217
ATAACGC1201.7118384E-423.7488653
TAAGGTG8550.023.3315325
CGCAGAG98900.023.0998338
TACATGG64950.022.9647522
GTACATG67250.022.5365031
AGGTAAG7300.022.124192
ACATGGG64950.021.8657763
GAGTACT57700.021.36100212-13
AGAGTAC89950.020.69878810-11
GTAAGGT8550.020.5539684
AGTACTT61300.020.22274212-13
Produced by FastQC (version 0.11.5)