##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938496_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1695777 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.708969988388805 31.0 31.0 34.0 30.0 34.0 2 31.9736987823281 31.0 31.0 34.0 30.0 34.0 3 32.026407363704074 33.0 31.0 34.0 30.0 34.0 4 35.708373211807924 37.0 35.0 37.0 33.0 37.0 5 35.58902909993472 37.0 35.0 37.0 33.0 37.0 6 35.624102697465524 37.0 35.0 37.0 33.0 37.0 7 35.5401382375159 37.0 35.0 37.0 33.0 37.0 8 35.50813638821614 37.0 35.0 37.0 33.0 37.0 9 37.033168276253306 39.0 37.0 39.0 33.0 39.0 10-11 36.95054184600923 39.0 37.0 39.0 33.0 39.0 12-13 36.81628745996673 39.0 37.0 39.0 32.0 39.0 14-15 37.77925694239278 39.5 37.0 41.0 32.0 41.0 16-17 37.871556519518776 40.0 37.0 41.0 32.5 41.0 18-19 38.00531614711133 40.0 37.0 41.0 33.0 41.0 20-21 37.95019274350342 40.0 37.0 41.0 33.0 41.0 22-23 37.81121515387932 40.0 37.0 41.0 32.0 41.0 24-25 37.675633942434644 39.5 37.0 41.0 32.0 41.0 26-27 37.43486643585801 39.0 36.0 41.0 32.0 41.0 28-29 37.280627110758076 39.0 36.0 41.0 31.5 41.0 30-31 36.99866639304578 39.0 36.0 40.0 31.0 41.0 32-33 36.88867463115728 39.0 35.5 40.0 30.0 41.0 34-35 36.746286805399535 39.0 35.0 40.0 30.0 41.0 36-37 36.59834990095986 39.0 35.0 40.0 30.0 41.0 38-39 36.366562348705045 38.0 35.0 40.0 30.0 41.0 40-41 36.11894400030192 38.0 35.0 40.0 29.5 41.0 42-43 35.81956884661132 38.0 35.0 40.0 29.0 41.0 44-45 35.495269130316075 38.0 34.0 40.0 27.5 41.0 46-47 35.04388401305124 38.0 34.0 40.0 26.5 41.0 48-49 34.579088229171646 38.0 33.0 40.0 24.5 41.0 50-51 34.13360748494643 37.5 33.0 40.0 24.0 41.0 52-53 33.47399156846684 37.0 32.0 39.5 22.5 41.0 54-55 32.98592945888522 36.5 31.0 39.0 20.5 40.0 56-57 32.5406430208689 36.0 31.0 39.0 19.0 40.0 58-59 31.941019662373062 35.5 30.5 39.0 16.5 40.0 60-61 31.369578075419113 35.0 29.5 38.0 13.0 40.0 62-63 30.786627604926828 34.5 28.5 38.0 8.0 40.0 64-65 30.053570133337107 34.0 28.0 37.5 7.0 40.0 66-67 29.331987047825272 34.0 26.5 37.0 2.0 39.0 68-69 28.54361540461983 33.0 26.0 36.0 2.0 39.0 70-71 27.701476078517402 32.5 25.5 35.5 2.0 38.5 72-73 27.06345675168374 32.0 24.0 35.0 2.0 38.0 74-75 26.309252926534562 31.0 22.0 35.0 2.0 37.0 76-77 25.239265245371293 30.5 21.5 34.0 2.0 36.0 78-79 25.703747603605898 31.0 21.5 34.0 2.0 36.0 80-81 25.306296464688458 31.0 20.0 34.0 2.0 36.0 82-83 24.575771755366418 31.0 17.5 34.0 2.0 35.5 84-85 23.736741623456385 30.0 9.5 34.0 2.0 35.0 86-87 22.768595752861373 29.5 4.5 34.0 2.0 35.0 88-89 21.708137036886335 29.0 2.0 34.0 2.0 35.0 90-91 20.78151460952708 27.5 2.0 33.0 2.0 35.0 92-93 19.638958719218387 26.0 2.0 33.0 2.0 34.0 94-95 18.399798145628818 24.5 2.0 32.0 2.0 34.0 96-97 16.970531207818006 19.5 2.0 32.0 2.0 34.0 98-99 15.218507504229624 2.0 2.0 31.0 2.0 34.0 100-101 11.386648716193225 2.0 2.0 25.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3293617702357885 1101 2 0.2594957061662164 1101 3 0.29689214660676555 1101 4 0.2299995214183994 1101 5 0.13440308826157832 1101 6 0.2798998974790621 1101 7 0.21682533407287963 1101 8 0.2239393174337252 1101 9 0.3423387699581326 1101 10-11 0.32139273477955754 1101 12-13 0.22776549610531305 1101 14-15 0.38126426330796903 1101 16-17 0.38486960559655614 1101 18-19 0.5008734961811285 1101 20-21 0.4564892935423046 1101 22-23 0.3577939456134942 1101 24-25 0.37462763232061036 1101 26-27 0.5290119118996088 1101 28-29 0.5302175527253894 1101 30-31 0.57341711514038 1101 32-33 0.4589503996339843 1101 34-35 0.34718439147206226 1101 36-37 0.46235983758965915 1101 38-39 0.67915590896596 1101 40-41 0.946409109302266 1101 42-43 0.8057213346409995 1101 44-45 0.8900085145366745 1101 46-47 0.8213155205472589 1101 48-49 0.7523015584195107 1101 50-51 1.0340084441888706 1101 52-53 1.296521057829267 1101 54-55 1.0378616943078072 1101 56-57 1.2258301926407214 1101 58-59 1.1026927935465274 1101 60-61 1.2764983992756989 1101 62-63 1.4530239194849948 1101 64-65 1.2672236422145744 1101 66-67 1.532681539170845 1101 68-69 1.603519270612761 1101 70-71 1.900096619355086 1101 72-73 2.044650801502648 1101 74-75 1.9129678622343143 1101 76-77 1.9210666067743531 1101 78-79 1.9793287045328078 1101 80-81 2.2124507071186628 1101 82-83 2.377300861640979 1101 84-85 2.7597874458998923 1101 86-87 2.512577155378999 1101 88-89 2.2735324380889708 1101 90-91 2.606594444710929 1101 92-93 3.0061968990842907 1101 94-95 2.8407461736730895 1101 96-97 2.7572030378973267 1101 98-99 2.869599161271333 1101 100-101 2.282681553324963 1102 1 0.21821449636862766 1102 2 0.23331873885144105 1102 3 0.2294837261785645 1102 4 0.21573227100139292 1102 5 0.15625617219707522 1102 6 0.22359205228208623 1102 7 0.32439701543587773 1102 8 0.29276886463081553 1102 9 0.28160307345823554 1102 10-11 0.328116573372661 1102 12-13 0.28233735515262026 1102 14-15 0.5266030752988442 1102 16-17 0.224147191955133 1102 18-19 0.3181131860576727 1102 20-21 0.2296535381736149 1102 22-23 0.2648228031451083 1102 24-25 0.33115634850452125 1102 26-27 0.4739862798076686 1102 28-29 0.3108937406284582 1102 30-31 0.3056614627103613 1102 32-33 0.21319856724279163 1102 34-35 0.5529737705401558 1102 36-37 0.5502673249144365 1102 38-39 0.5452650432956503 1102 40-41 0.6606567483784858 1102 42-43 0.4263086525710804 1102 44-45 0.5686641779893762 1102 46-47 0.4779473701296624 1102 48-49 0.6539703305235562 1102 50-51 0.7343901003526909 1102 52-53 0.7011077123118667 1102 54-55 0.7418691883435571 1102 56-57 0.8305622283570031 1102 58-59 1.1108109845602918 1102 60-61 1.137691485095825 1102 62-63 1.2834727514453625 1102 64-65 1.3713937643368972 1102 66-67 1.642874846487853 1102 68-69 1.764184310650844 1102 70-71 1.9841209568899068 1102 72-73 1.902911574613693 1102 74-75 1.9707717676033347 1102 76-77 1.9680966187324067 1102 78-79 2.0134886002449797 1102 80-81 2.2033870686655526 1102 82-83 2.5175120382275438 1102 84-85 2.503436101080016 1102 86-87 2.3871367260436323 1102 88-89 2.2472608912583745 1102 90-91 2.3275019421195537 1102 92-93 2.7166211960055513 1102 94-95 2.451354831106432 1102 96-97 2.608157717271034 1102 98-99 2.7438377874760516 1102 100-101 2.429046135799867 1103 1 0.33284411358252797 1103 2 0.3131865586914664 1103 3 0.31577939717335113 1103 4 0.26866122162582684 1103 5 0.23352549181260684 1103 6 0.323040387366035 1103 7 0.3004565459450603 1103 8 0.2635494508475844 1103 9 0.3830685138115797 1103 10-11 0.43945370727499977 1103 12-13 0.49863033080446684 1103 14-15 0.5686875031919811 1103 16-17 0.4867172759509586 1103 18-19 0.47895185081999614 1103 20-21 0.5731836315421859 1103 22-23 0.7104501848496909 1103 24-25 0.4691103326345001 1103 26-27 0.5717181961220064 1103 28-29 0.5961651474417096 1103 30-31 0.5331372334057534 1103 32-33 0.517681064782785 1103 34-35 0.4734034203961457 1103 36-37 0.6787019401609982 1103 38-39 0.6095333965156087 1103 40-41 0.7001055981510405 1103 42-43 0.7163167182832737 1103 44-45 0.6199195441259846 1103 46-47 0.7966376433954494 1103 48-49 0.8453076811368874 1103 50-51 0.8361611370642095 1103 52-53 0.9299585819662965 1103 54-55 0.8578305623183269 1103 56-57 1.2152269459792038 1103 58-59 1.2342282324526117 1103 60-61 1.5008947037958187 1103 62-63 1.5099198221561316 1103 64-65 1.758411319958821 1103 66-67 1.6655269014159657 1103 68-69 1.732318483197492 1103 70-71 1.7522401966948387 1103 72-73 1.8603586785083266 1103 74-75 1.727880565324618 1103 76-77 1.7985151892389872 1103 78-79 1.96933779919312 1103 80-81 2.30550484296241 1103 82-83 2.3743466626843244 1103 84-85 2.3935304448554326 1103 86-87 2.403780976667182 1103 88-89 2.3417710939441037 1103 90-91 2.6630971964197414 1103 92-93 2.974067419511787 1103 94-95 2.657863305522003 1103 96-97 2.9053037012438985 1103 98-99 2.892188989307799 1103 100-101 2.3875688372919885 1104 1 0.20283094774179133 1104 2 0.19555814288542095 1104 3 0.25891547307530516 1104 4 0.14109784025227157 1104 5 0.181487848322206 1104 6 0.11169932824873996 1104 7 0.1357128563957204 1104 8 0.18842012138040332 1104 9 0.26615897505899255 1104 10-11 0.16088135736460885 1104 12-13 0.2203840325164137 1104 14-15 0.34838751615587427 1104 16-17 0.3178867035788997 1104 18-19 0.23343709641406463 1104 20-21 0.27410813935407674 1104 22-23 0.5138584829123332 1104 24-25 0.38580870328842565 1104 26-27 0.5504085565246797 1104 28-29 0.3284948432853838 1104 30-31 0.47542674770284066 1104 32-33 0.6486416043933332 1104 34-35 0.40396794246179013 1104 36-37 0.5148753318731067 1104 38-39 0.512200635591249 1104 40-41 0.4440521452285324 1104 42-43 0.5707393919326336 1104 44-45 0.5144633422071934 1104 46-47 0.578018727710905 1104 48-49 0.5986851329571365 1104 50-51 0.6273394048929859 1104 52-53 0.7093483410138219 1104 54-55 0.7216836521216123 1104 56-57 1.0025986181191442 1104 58-59 1.004713296943077 1104 60-61 0.9933977965632117 1104 62-63 1.303085180816165 1104 64-65 1.4257088633992545 1104 66-67 1.4756651850880438 1104 68-69 1.3666988189948306 1104 70-71 1.4431237151599134 1104 72-73 1.8437059420524982 1104 74-75 1.8356412779607645 1104 76-77 1.9318240496999657 1104 78-79 1.8676425913376526 1104 80-81 2.0641554713202375 1104 82-83 2.0771940933258826 1104 84-85 2.2528028513769875 1104 86-87 2.196343253829955 1104 88-89 2.4898126995643075 1104 90-91 2.812627857975553 1104 92-93 3.175827902836904 1104 94-95 3.049762209324072 1104 96-97 3.217400900457207 1104 98-99 3.0807259784148773 1104 100-101 2.8831837524110764 1105 1 0.1483921557004919 1105 2 0.23527576817443574 1105 3 0.28407317145155275 1105 4 0.24935004628557778 1105 5 0.14567332859816418 1105 6 0.0875907596821861 1105 7 0.19988631252425648 1105 8 0.14672447794624333 1105 9 0.3166282686602173 1105 10-11 0.24064168826424748 1105 12-13 0.3306990070469453 1105 14-15 0.3608758017908684 1105 16-17 0.2897598362792806 1105 18-19 0.21680598716045552 1105 20-21 0.326254388477885 1105 22-23 0.351393281804647 1105 24-25 0.3554625700002916 1105 26-27 0.4338673377167126 1105 28-29 0.2517956216730113 1105 30-31 0.36560069397601325 1105 32-33 0.44386976182583027 1105 34-35 0.49950453610343715 1105 36-37 0.5264436019531971 1105 38-39 0.43841245089177505 1105 40-41 0.629194949571783 1105 42-43 0.5500242029867692 1105 44-45 0.7820381022673217 1105 46-47 0.7392443401851736 1105 48-49 0.702941069229766 1105 50-51 0.9024105936589351 1105 52-53 1.033673405425617 1105 54-55 1.0136677374129022 1105 56-57 1.0689775721284676 1105 58-59 1.172926071115814 1105 60-61 1.2652779968922339 1105 62-63 1.5038295459121152 1105 64-65 1.5744677602739863 1105 66-67 1.7198101442931382 1105 68-69 1.7199000417251824 1105 70-71 1.6707399080455936 1105 72-73 1.9746154051177136 1105 74-75 2.1315587586540516 1105 76-77 1.962628355017852 1105 78-79 1.9788349002890975 1105 80-81 2.0561935275938588 1105 82-83 2.24271306073004 1105 84-85 2.610855955274495 1105 86-87 2.548738430341686 1105 88-89 2.7849655913411695 1105 90-91 2.783056257237213 1105 92-93 2.729367331484905 1105 94-95 2.6121139918197898 1105 96-97 2.40277332624175 1105 98-99 1.9964839754672763 1105 100-101 1.8035020527045216 1106 1 0.17298722607471007 1106 2 0.0823116714212837 1106 3 0.0733536397815584 1106 4 0.16723068104861483 1106 5 0.07962307606698005 1106 6 0.006600565448309226 1106 7 0.057581492461295625 1106 8 0.1425862171623251 1106 9 -0.06948692263866718 1106 10-11 -0.003457608039305171 1106 12-13 0.05798789582741648 1106 14-15 0.12879075688780262 1106 16-17 0.19037144262032513 1106 18-19 0.28869816733870834 1106 20-21 0.17016227764165848 1106 22-23 0.3189219158797485 1106 24-25 0.22833096623288895 1106 26-27 0.08421830162867394 1106 28-29 0.28712101838465287 1106 30-31 0.2644179155566846 1106 32-33 0.10139216609710644 1106 34-35 0.06214150869320889 1106 36-37 0.1408141706888486 1106 38-39 0.10211708927499075 1106 40-41 0.16555849445519044 1106 42-43 0.32881539539196325 1106 44-45 0.26626752005358156 1106 46-47 -0.07293485007449618 1106 48-49 0.16702105100690545 1106 50-51 0.3136676021882536 1106 52-53 0.206535062614428 1106 54-55 0.25530956973756247 1106 56-57 0.43502687936391027 1106 58-59 0.6728402321643223 1106 60-61 0.6757552889065899 1106 62-63 0.7209133573567215 1106 64-65 0.9308941093863368 1106 66-67 0.7338948982890727 1106 68-69 0.7020908225303124 1106 70-71 0.7228074070400758 1106 72-73 0.9850837930561056 1106 74-75 1.0866358658173496 1106 76-77 1.1949378314246424 1106 78-79 1.4373478525178136 1106 80-81 1.60303186403193 1106 82-83 1.798270835528271 1106 84-85 2.115832022521001 1106 86-87 2.1796538089920894 1106 88-89 1.7178291635161713 1106 90-91 1.8247713957215126 1106 92-93 1.8632172435266625 1106 94-95 1.921887251964872 1106 96-97 1.911970748816696 1106 98-99 1.940053631718106 1106 100-101 1.9606088317861623 1107 1 0.10521063662750407 1107 2 0.06509999951224543 1107 3 0.10437717683599601 1107 4 0.10501211492012175 1107 5 0.04030605279602639 1107 6 0.03155700917835702 1107 7 0.0383993141762744 1107 8 0.17238948788455843 1107 9 0.1815317429612051 1107 10-11 0.0940007661022193 1107 12-13 0.18696641307317208 1107 14-15 0.31656295541849744 1107 16-17 0.27494644124629275 1107 18-19 0.18495540656947185 1107 20-21 0.31878188701827526 1107 22-23 0.3334161450681705 1107 24-25 0.37790789752286 1107 26-27 0.3486908315203934 1107 28-29 0.25285054573643606 1107 30-31 0.27450065520026357 1107 32-33 0.1566942878828712 1107 34-35 0.3105703744942545 1107 36-37 0.35921060918103365 1107 38-39 0.3285185409804612 1107 40-41 0.3245279926022704 1107 42-43 0.10548353070306149 1107 44-45 0.13714709391243218 1107 46-47 0.29008267064345006 1107 48-49 0.3602038077396159 1107 50-51 0.42621321951597224 1107 52-53 0.44619140582192074 1107 54-55 0.30267350313378927 1107 56-57 0.43242011056489105 1107 58-59 0.6308676354477818 1107 60-61 0.7113738209769487 1107 62-63 0.9062636440727552 1107 64-65 1.1050124196583475 1107 66-67 0.9159282710565613 1107 68-69 1.3658955847225371 1107 70-71 1.243527607448474 1107 72-73 1.4215496038244204 1107 74-75 1.374443173659941 1107 76-77 1.6019380996249488 1107 78-79 1.6892820932872041 1107 80-81 1.8232091465351488 1107 82-83 1.8480020195350058 1107 84-85 1.7992748734812238 1107 86-87 2.0699124315717903 1107 88-89 2.0313982668411654 1107 90-91 2.335705889073722 1107 92-93 2.3319252058320536 1107 94-95 1.9619336419076916 1107 96-97 2.007426180263284 1107 98-99 1.9822888725172287 1107 100-101 1.5658818059353177 1108 1 0.09920807361876527 1108 2 0.12538550703326123 1108 3 0.07421726135817153 1108 4 0.08133125248469497 1108 5 0.03531011824821917 1108 6 0.0017755167781103864 1108 7 0.07436998523402849 1108 8 0.011807443194292944 1108 9 0.06199475744136862 1108 10-11 0.08520012209074679 1108 12-13 0.12914123242086362 1108 14-15 0.28983019162343737 1108 16-17 0.21203158557344892 1108 18-19 0.05272365358513298 1108 20-21 0.13788296491834018 1108 22-23 0.19679892433762802 1108 24-25 0.21721322004921717 1108 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0.021607596451072908 2316 74-75 0.16316031274057252 2316 76-77 0.1895286189602139 2316 78-79 0.10757180034912395 2316 80-81 -0.2495179379043755 2316 82-83 0.19969482054121457 2316 84-85 0.16167983523997975 2316 86-87 0.35321159067870767 2316 88-89 0.407870206317952 2316 90-91 0.547582327121404 2316 92-93 0.6498250631099403 2316 94-95 0.8539449692986629 2316 96-97 1.0102500257769549 2316 98-99 0.9784543925115035 2316 100-101 0.6911242264119881 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 19.0 4 33.0 5 57.0 6 153.0 7 406.0 8 968.0 9 1665.0 10 2820.0 11 4287.0 12 6257.0 13 8359.0 14 11582.0 15 15462.0 16 19336.0 17 23934.0 18 24577.0 19 23627.0 20 25062.0 21 27362.0 22 30259.0 23 32816.0 24 36732.0 25 41516.0 26 46915.0 27 54600.0 28 63879.0 29 73605.0 30 84616.0 31 97336.0 32 111955.0 33 130714.0 34 153257.0 35 176865.0 36 186887.0 37 141180.0 38 36182.0 39 494.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.408261155769054 19.077880987827925 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28.091569695439972 23.18425162116705 44-45 22.73008588756628 26.0538689504757 28.195677050484928 23.02036811147309 46-47 23.29447500568324 26.272434057456305 27.629276398910115 22.803814537950338 48-49 22.974807418664128 26.43145295637339 27.541740452901532 23.05199917206095 50-51 23.149358671570614 26.384453852127965 27.50388170142654 22.96230577487488 52-53 23.444326700975424 26.168387706638313 27.322195076357325 23.065090516028935 54-55 23.161034735109627 26.503101528090074 27.257387026714007 23.07847671008629 56-57 23.03747664785915 26.687339601456795 27.12236167984451 23.152822070839544 58-59 23.10144553204814 26.266543301389273 27.29633672351966 23.335674443042922 60-61 22.81325905469882 26.42384582406767 27.55860587801344 23.20428924322007 62-63 22.8334562858206 26.492988170024713 27.67745994903811 22.996095595116575 64-65 22.798327724781643 26.553103937524675 27.261128918455206 23.387439419238476 66-67 22.607440605627264 26.63279793013467 27.248538636748954 23.51122282748911 68-69 23.10618167493634 26.333182761178804 27.27816062943092 23.282474934453937 70-71 23.108267964952876 26.276939026073048 26.918858291258154 23.69593471771592 72-73 22.8529020566745 26.467893817062027 26.974595339053643 23.704608787209832 74-75 22.73898631718675 26.68490609319504 26.86927585407751 23.7068317355407 76-77 22.927218614239962 26.409899414840517 27.079946242931708 23.58293572798782 78-79 22.774309357893166 26.63265865735884 27.14861682874576 23.444415156002236 80-81 22.89693502970618 26.40878064601731 27.020153027376864 23.67413129689965 82-83 22.888579357619086 26.42715275918559 27.103518552808957 23.580749330386368 84-85 22.7657019787955 26.54979306212618 26.961073977488653 23.723430981589665 86-87 22.966991653926165 26.518694998990096 26.677507765932507 23.83680558115123 88-89 22.8191560564862 26.627734660866377 26.4843490624062 24.068760220241224 90-91 22.583979876732897 26.42228490840475 26.872941358933826 24.120793855928518 92-93 22.759946301632564 26.234683566772233 26.697688982900676 24.30768114869453 94-95 22.72206862166093 25.970020223773595 26.865067418967065 24.442843735598412 96-97 22.58560530069697 25.998347660099174 26.826728986181557 24.589318053022303 98-99 22.410759197701115 26.105024422432905 26.558061584748465 24.92615479511752 100-101 22.79643655765856 25.610310559885267 26.795343252882457 24.797909629573716 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 358.0 1 275.0 2 240.5 3 327.0 4 307.0 5 274.5 6 502.5 7 1181.0 8 1857.5 9 2030.0 10 1720.5 11 1430.5 12 1470.5 13 1574.0 14 1691.0 15 1771.0 16 1870.0 17 1993.5 18 2134.0 19 2353.0 20 2604.0 21 2985.0 22 3556.5 23 4285.0 24 5170.5 25 6368.5 26 8085.0 27 10085.0 28 11877.5 29 13835.0 30 16292.0 31 19808.0 32 24191.0 33 26493.5 34 27612.0 35 29636.5 36 32399.0 37 35552.5 38 38271.0 39 40458.0 40 42497.0 41 45179.5 42 47597.5 43 50714.0 44 54493.5 45 57104.0 46 58439.0 47 61404.5 48 64641.5 49 65635.0 50 65313.0 51 64643.5 52 64316.0 53 64408.5 54 65139.0 55 62454.0 56 58026.0 57 54029.0 58 49768.5 59 44348.0 60 36409.0 61 30075.0 62 25343.0 63 19360.5 64 14034.0 65 10537.5 66 8170.0 67 6707.0 68 5343.5 69 4264.5 70 3336.5 71 2169.0 72 1544.5 73 1169.0 74 775.5 75 537.5 76 399.5 77 281.5 78 185.0 79 103.0 80 52.5 81 31.5 82 21.0 83 9.0 84 6.5 85 4.0 86 4.0 87 1.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024826377524875026 2 0.011027393342402922 3 0.0018870405719619973 4 0.0 5 2.3588007149524966E-4 6 0.0 7 0.0 8 5.8970017873812415E-5 9 2.948500893690621E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0018575555630250913 26-27 0.008462197564892082 28-29 2.653650804321559E-4 30-31 0.0 32-33 3.538201072428745E-4 34-35 0.010054388047485017 36-37 0.0011499153485393422 38-39 0.01220679369987917 40-41 0.012177308690942265 42-43 0.005012451519274055 44-45 7.960952412964676E-4 46-47 8.845502681071862E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 5.8970017873812415E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 2.0639506255834347E-4 66-67 8.5506525917028E-4 68-69 0.0013563104110976855 70-71 1.7691005362143725E-4 72-73 3.243350983059683E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.1794003574762483E-4 82-83 0.0013563104110976855 84-85 0.00583803176950743 86-87 0.004098416242229963 88-89 0.0 90-91 0.001680645509403654 92-93 5.012451519274055E-4 94-95 2.9485008936906208E-5 96-97 0.0 98-99 0.0 100-101 5.8970017873812415E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1695777.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.5155797696459 #Duplication Level Percentage of deduplicated Percentage of total 1 76.76741443933226 41.082526911375524 2 12.811607907293565 13.712412498803902 3 4.311740359426984 6.922358554527492 4 1.9017058401255564 4.070835623425627 5 1.0312816615812828 2.759481801266305 6 0.6644992636519853 2.1336638010503273 7 0.4432184378359362 1.660336416577082 8 0.32424258430417907 1.3881623908037146 9 0.24725732287715366 1.190890708745527 >10 1.3407467442633685 12.96767031379806 >50 0.09588504925488237 3.4998375634824037 >100 0.054980154865017394 5.515683471631923 >500 0.003982213607418309 1.515393033527686 >1k 0.0013274045358061032 1.2457490916659488 >5k 1.106170446505086E-4 0.3349978193184568 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5659 0.33371133114790447 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4730 0.27892818454313273 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3366 0.19849308016325262 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.8970017873812415E-5 7 0.0 0.0 0.0 0.0 5.8970017873812415E-5 8 0.0 0.0 0.0 0.0 5.8970017873812415E-5 9 0.0 0.0 0.0 5.8970017873812415E-5 5.8970017873812415E-5 10-11 0.0 0.0 0.0 5.8970017873812415E-5 5.8970017873812415E-5 12-13 0.0 0.0 0.0 5.8970017873812415E-5 5.8970017873812415E-5 14-15 0.0 0.0 0.0 5.8970017873812415E-5 5.8970017873812415E-5 16-17 0.0 0.0 0.0 5.8970017873812415E-5 5.8970017873812415E-5 18-19 0.0 0.0 0.0 5.8970017873812415E-5 5.8970017873812415E-5 20-21 0.0 0.0 0.0 1.4742504468453105E-4 5.8970017873812415E-5 22-23 0.0 0.0 0.0 2.0639506255834344E-4 5.8970017873812415E-5 24-25 0.0 0.0 0.0 2.948500893690621E-4 5.8970017873812415E-5 26-27 0.0 0.0 0.0 9.435202859809986E-4 5.8970017873812415E-5 28-29 0.0 0.0 0.0 0.0036266560992394637 5.8970017873812415E-5 30-31 0.0 0.0 0.0 0.01111584836921364 5.8970017873812415E-5 32-33 0.0 0.0 0.0 0.0261237179180989 1.1794003574762483E-4 34-35 0.0 0.0 0.0 0.05062576034466796 1.1794003574762483E-4 36-37 0.0 0.0 0.0 0.08880884691796151 1.1794003574762483E-4 38-39 0.0 0.0 0.0 0.14984281541735736 1.1794003574762483E-4 40-41 0.0 0.0 0.0 0.2170096657756297 1.1794003574762483E-4 42-43 0.0 0.0 0.0 0.2835868159551639 1.1794003574762483E-4 44-45 0.0 0.0 0.0 0.37053810731010034 1.7691005362143725E-4 46-47 0.0 0.0 0.0 0.4794557303230319 2.3588007149524966E-4 48-49 0.0 0.0 0.0 0.5906142140151682 2.3588007149524966E-4 50-51 0.0 0.0 0.0 0.7105297453615658 2.3588007149524966E-4 52-53 0.0 0.0 0.0 0.8285287511270645 2.3588007149524966E-4 54-55 0.0 0.0 0.0 0.96009086100354 2.3588007149524966E-4 56-57 0.0 0.0 0.0 1.1181305089053573 2.3588007149524966E-4 58-59 0.0 0.0 0.0 1.2896742909002774 2.3588007149524966E-4 60-61 0.0 0.0 0.0 1.473719716684446 2.3588007149524966E-4 62-63 0.0 0.0 0.0 1.659563728013766 2.3588007149524966E-4 64-65 0.0 0.0 0.0 1.851894441309205 2.3588007149524966E-4 66-67 0.0 0.0 0.0 2.074181923684541 2.3588007149524966E-4 68-69 0.0 0.0 0.0 2.327811970559808 2.3588007149524966E-4 70-71 0.0 0.0 0.0 2.597334437252068 2.3588007149524966E-4 72-73 0.0 0.0 0.0 2.873579485981942 2.3588007149524966E-4 74-75 0.0 0.0 0.0 3.161382658215084 2.653650804321559E-4 76-77 2.9485008936906208E-5 0.0 0.0 3.4679677811410343 2.948500893690621E-4 78-79 5.8970017873812415E-5 0.0 0.0 3.8118809253811086 2.948500893690621E-4 80-81 5.8970017873812415E-5 0.0 0.0 4.179411561779644 2.948500893690621E-4 82-83 5.8970017873812415E-5 2.9485008936906208E-5 0.0 4.5604168472623465 3.538201072428745E-4 84-85 5.8970017873812415E-5 5.8970017873812415E-5 0.0 4.947289649523492 3.833051161797807E-4 86-87 5.8970017873812415E-5 5.8970017873812415E-5 0.0 5.348904956253092 4.1279012511668693E-4 88-89 5.8970017873812415E-5 1.1794003574762483E-4 0.0 5.776467070847168 4.1279012511668693E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3595 0.0 48.50151 1 GTATCAA 6170 0.0 38.193073 1 ATCAACG 7980 0.0 29.224792 3 TATCAAC 8080 0.0 28.924444 2 TCAACGC 8155 0.0 28.596807 4 CAACGCA 8265 0.0 28.216211 5 AACGCAG 8500 0.0 27.436115 6 GTGTACG 255 1.8189894E-11 24.220957 1 ACGCAGA 9710 0.0 23.772621 7 ATAACGC 120 1.7118384E-4 23.748865 3 TAAGGTG 855 0.0 23.331532 5 CGCAGAG 9890 0.0 23.099833 8 TACATGG 6495 0.0 22.964752 2 GTACATG 6725 0.0 22.536503 1 AGGTAAG 730 0.0 22.12419 2 ACATGGG 6495 0.0 21.865776 3 GAGTACT 5770 0.0 21.361002 12-13 AGAGTAC 8995 0.0 20.698788 10-11 GTAAGGT 855 0.0 20.553968 4 AGTACTT 6130 0.0 20.222742 12-13 >>END_MODULE